8-ethyl-6-(aryl)pyrido[2,3-d]pyrimidin-7(8h)-ones for the treatment of nervous system disorders and cancer

ABSTRACT

Provided herein are PAK inhibitors and methods of utilizing PAK inhibitors for the treatment of CNS disorders such as neuropsychiatric disorders or neurofibromatosis. Also described herein are methods of utilizing PAK inhibitors for the treatment of cancer.

CROSS-REFERENCE

This application claims the benefit of U.S. provisional application Ser. No. 61/473,683, filed Apr. 8, 2011; which is incorporated by reference in its entirety.

BACKGROUND OF THE INVENTION

Nervous System disorders are characterized by a variety of debilitating affective and cognitive impairments and can be classified as central nervous system (CNS) disorders and peripheral nervous system (PNS) disorders.

Neurofibromatosis type 1 (NF1) affects nerves of the peripheral nervous system. A neurofibroma is a benign nerve sheath tumor in the PNS and can result in a range of symptoms from physical disfiguration and pain to cognitive disorder. Neurofibromatosis type 2 (NF2) affects the CNS and can cause tumors in the brain and spinal cord.

Cancer, also called malignancy, is characterized by an abnormal growth of cells. There are more than 100 types of cancer, including breast cancer, skin cancer, lung cancer, colon cancer, brain cancer, prostate cancer, kidney cancer, ovarian cancer, cancers of the central nervous system, leukemia, and lymphoma. Cancer symptoms vary widely based on the type of cancer. Cancer treatment includes chemotherapy, radiation, and surgery.

A number of cancers have been associated with alterations in the expression and/or activation of p21-activated kinases, which are central players in growth factor signaling networks and oncogenic processes that control cell proliferation, cell polarity, invasion and actin cytoskeleton organization.

The effects of cancer and nervous system disorders are devastating to the quality of life of those afflicted as well as that of their families. Moreover, cancer and nervous system disorders impose an enormous health care burden on society.

SUMMARY OF THE INVENTION

Described herein are compounds and compositions for the treatment of p21-activated kinase (PAK) mediated conditions or disorders. Also described herein are methods for treating nervous system conditions or disorders. In one embodiment, the compounds and compositions described herein are used to treat peripheral nervous system (PNS) disorders or conditions. In another embodiment, the compounds and compositions described herein are used to treat central nervous system (CNS) disorders or conditions.

Also described herein are compounds, compositions and methods for treating an individual with cancer or a non-malignant tumor. In one embodiment, the individual suffers from a cancer (e.g., including breast cancer, skin cancer, lung cancer, colon cancer, brain cancer, prostate cancer, kidney cancer, liver cancer, cancer of the central nervous system, and lymphoma or the like) by administering to an individual a pharmaceutical composition comprising a therapeutically effective amount of an inhibitor of a p21-activated kinase (PAK), e.g., an inhibitor of PAK1, PAK2, PAK3, PAK4, PAK5, or PAK6, as described herein. In another embodiment the individual suffers from a non-malignant tumor.

Also described herein are compounds, compositions and methods for treating an individual suffering from a CNS disorder, such as by way of example only schizophrenia, Fragile X Syndrome (FXS), clinical depression, age-related cognitive decline, Mild Cognitive Impairment, Huntington's disease, Parkinson's disease, neurofibromatosis, Alzheimer's disease, epilepsy, autism spectrum disorders, mental retardation, Down's syndrome or the like, by administering to an individual a pharmaceutical composition comprising a therapeutically effective amount of an inhibitor of a p21-activated kinase (PAK), e.g., an inhibitor of PAK1, PAK2, PAK3 or PAK4, as described herein. PAK activation is shown to play a key role in spine morphogenesis. In some instances, attenuation of PAK activity reduces, prevents or reverses defects in spine morphogenesis. In some embodiments, inhibitors of one or more of Group I PAKs (PAK1, PAK2 and/or PAK3) and/or Group II PAKs (PAK4, PAK5 and/or PAK6) are administered to rescue defects in spine morphogenesis in individuals suffering from a condition in which dendritic spine morphology, density, and/or function are aberrant, including but not limited to abnormal spine density, spine size, spine shape, spine plasticity, spine motility or spine plasticity leading to improvements in synaptic function, cognition and/or behavior.

In one aspect is a compound having the structure of Formula I or pharmaceutically acceptable salt or N-oxide thereof

wherein:

-   -   wherein ring T is an aryl, or a heteroaryl ring;     -   R³ is a substituted or unsubstituted cycloalkyl, a substituted         or unsubstituted heteroaryl attached to ring T via a carbon atom         of R³, or a substituted or unsubstituted heterocycloalkyl         attached to ring T via a carbon atom of R³;     -   Q is a substituted or unsubstituted alkyl, a substituted or         unsubstituted heteroalkyl, a substituted or unsubstituted         heterocycloalkyl, a substituted or unsubstituted cycloalkyl, a         substituted or unsubstituted cycloalkylalkyl, a substituted or         unsubstituted heterocycloalkylalkyl, a substituted or         unsubstituted aryl, a substituted or unsubstituted arylalkyl, a         substituted or unsubstituted heteroaryl, or a substituted or         unsubstituted heteroarylalkyl;     -   each R⁴ is independently halogen, —CN, —NO₂, —OH, —OCF₃, —OCH₂F,         —OCF₂H, —CF₃, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)OR¹⁰,         —NR¹⁰C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)N(R¹⁰)₂, a substituted or         unsubstituted alkyl, a substituted or unsubstituted alkoxy, a         substituted or unsubstituted heteroalkyl, a substituted or         unsubstituted cycloalkyl, or a substituted or unsubstituted         heterocycloalkyl;     -   R⁸ is H or R⁹;     -   R⁹ is a substituted or unsubstituted alkyl, a substituted or         unsubstituted cycloalkyl, a substituted or unsubstituted         heterocycloalkyl, a substituted or unsubstituted aryl, or a         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, a substituted or unsubstituted         alkyl, a substituted or unsubstituted cycloalkyl, a substituted         or unsubstituted heterocycloalkyl, a substituted or         unsubstituted aryl, or a substituted or unsubstituted         heteroaryl; or two R¹⁰, together with the atoms to which they         are attached form a heterocycle;     -   ring B is aryl or heteroaryl;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰, S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, —OR¹⁰, a substituted or         unsubstituted alkyl, a substituted or unsubstituted alkoxy, a         substituted or unsubstituted heteroalkyl, a substituted or         unsubstituted cycloalkyl, or a substituted or unsubstituted         heterocycloalkyl;     -   r is 0 to 8; and     -   s is 0 to 4.

In one embodiment is a compound of Formula I wherein ring T is an aryl ring. In another embodiment is a compound of Formula I wherein ring T is a heteroaryl ring. In yet another embodiment is a compound of Formula I, wherein ring T is selected from pyrrole, furan, thiophene, pyrazole, imidazole, isoxazole, oxazole, isothiazole, thiazole, 1,2,3-triazole, 1,3,4-triazole, 1-oxa-2,3-diazole, 1-oxa-2,4-diazole, 1-oxa-2,5-diazole, 1-oxa-3,4-diazole, 1-thia-2,3-diazole, 1-thia-2,4-diazole, 1-thia-2,5-diazole, 1-thia-3,4-diazole, tetrazole, pyridine, pyridazine, pyrimidine, and pyrazine. In yet a further embodiment is a compound of Formula I, wherein R³ is a C-linked heterocycloalkyl. In another embodiment is a compound of Formula I, wherein R³ is a substituted or unsubstituted C-linked heteroaryl. In another embodiment is a compound of Formula I, wherein R³ is a substituted or unsubstituted cycloalkyl. In a further embodiment, cycloalkyl is selected from cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cyclohexyl.

In yet another embodiment is a compound having the structure of Formula II:

In a further embodiment is a compound having the structure of Formula III:

wherein s1 is 0 to 3.

In yet a further embodiment is a compound having the structure of Formula IV:

In another embodiment is a compound having the structure of Formula V:

In another embodiment is a compound having the structure of Formula Va:

In another embodiment is a compound having the structure of Formula Vb:

In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb wherein R³ is selected from pyrrole, furan, thiophene, pyrazole, imidazole, isoxazole, oxazole, isothiazole, thiazole, 1,2,3-triazole, 1,3,4-triazole, 1-oxa-2,3-diazole, 1-oxa-2,4-diazole, 1-oxa-2,5-diazole, 1-oxa-3,4-diazole, 1-thia-2,3-diazole, 1-thia-2,4-diazole, 1-thia-2,5-diazole, 1-thia-3,4-diazole, tetrazole, pyridine, pyridazine, pyrimidine, and pyrazine.

In a further embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein r is 0 to 7, and

In another embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, where R⁵ is halogen, —CN, —OH, a substituted or unsubstituted alkyl, —OR¹⁰, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, or a substituted or unsubstituted heterocycloalkyl.

In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein at least one R⁵ is —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, or a substituted or unsubstituted heterocycloalkyl.

In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein at least one R⁵ is —N(R¹⁰)₂, or a substituted or unsubstituted heterocycloalkyl. In a further embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb wherein at least one of R⁵ is a substituted or unsubstituted piperazine, a substituted or unsubstituted piperidine, a substituted or unsubstituted pyrrolidine, or a substituted or unsubstituted morpholine. In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein at least one R⁵ is —OR¹⁰. In another embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein R⁴ is independently halogen, —CN, —OH, —OCF₃, —OCF₃, —OCF₂H, —CF₃, —SR⁸, a substituted or unsubstituted alkyl, or a substituted or unsubstituted alkoxy.

In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein s is zero.

In a further embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein Q is a substituted or unsubstituted alkyl, or a substituted or unsubstituted heteroalkyl. In another embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein Q is a substituted or unsubstituted cycloalkyl, or a substituted or unsubstituted heterocycloalkyl. In a further embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein Q is a substituted or unsubstituted cycloalkylalkyl, or a substituted or unsubstituted heterocycloalkylalkyl. In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein Q is a substituted or unsubstituted aryl, or a substituted or unsubstituted heteroaryl.

In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein Q is a substituted or unsubstituted arylalkyl, or a substituted or unsubstituted heteroarylalkyl.

Provided herein are pharmaceutical compositions comprising a therapeutically effective amount of a compound of Formula I, II, III, IV, V, Va, or Vb, or a pharmaceutically acceptable salt or N-oxide thereof, and a pharmaceutically acceptable carrier, wherein the compound of Formula I, II, III, IV, V, Va, or Vb is as described herein.

Provided herein, in some embodiments, are methods for treating nervous system disorders comprising administering to an individual in need thereof a therapeutically effective amount of a compound of Formula I-XV, or a pharmaceutically acceptable salt, solvate, or N-oxide thereof, wherein compounds of Formula I-XV are as described herein.

In one embodiment, the nervous system disorder is a peripheral nervous system (PNS) disorder. In another embodiment, the nervous system disorder is a central nervous system (CNS) disorder.

Provided herein, in some embodiments, are methods for treating CNS disorders comprising administering to an individual in need thereof a therapeutically effective amount of a compound of Formula I-XV, or a pharmaceutically acceptable salt, solvate, or N-oxide thereof, wherein compounds of Formula I-XV are as described herein.

Also provided herein, in some embodiments, are methods for treating neuropsychiatric conditions comprising administering to an individual in need thereof a therapeutically effective amount of a compound of Formula I-XV, or a pharmaceutically acceptable salt, solvate, or N-oxide thereof, wherein compounds of Formula I-XV are as described herein.

Also provided herein, in some embodiments, are methods for treating neurodegenerative disorder comprising administering to an individual in need thereof a therapeutically effective amount of a compound of Formula I-XV, or a pharmaceutically acceptable salt, solvate, or N-oxide thereof, wherein compounds of Formula I-XV are as described herein.

Also provided herein, in some embodiments, are methods for treating neurodevelopmental disorder comprising administering to an individual in need thereof a therapeutically effective amount of a compound of Formula I-XV, or a pharmaceutically acceptable salt, solvate, or N-oxide thereof, wherein compounds of Formula I-XV are as described herein.

Also provided herein, in some embodiments, are methods for treating cancer comprising administering to an individual in need thereof a therapeutically effective amount of a compound of Formula I-XV, or a pharmaceutically acceptable salt, solvate, or N-oxide thereof, wherein compounds of Formula I-XV are as described herein.

In some embodiments, the cancer is selected from ovarian, breast, colon, brain, chronic myelogenous leukemia, renal cell carcinoma, gastric, leukemia, lung, melanoma, prostate, T-cell lymphoma, heptocellular, bladder, kidney, glioblastoma, mesothelioma, neuroma, meningioma, neuroblastoma, medulloblastoma, peripheral malignant nerve sheath tumor, ependymoma, chraniopharyngioma, astrocytoma, germinoma, glioma, mixed glioma, choroid plexus tumor, oligodendroglioma, peripheral neuroectodermal tumor, primitive neuroectodermal tumor (PNET), CNS lymphoma, pituitary adenoma, schwannoma, head and neck cancer, and esophageal cancer. In some embodiments, the cancer is selected from NSCLC, SCLC, or mesothelioma. In some embodiments, the cancer is an ovarian cancer. In some embodiments, the kidney cancer is a renal cell carcinoma. In some embodiments, the cancer is a meningioma. In some embodiments, the cancer is a head and neck cancer. In some embodiments, the cancer is an esophageal cancer. In some embodiments, the esophageal cancer is an esophageal squamous cancer. In some embodiments, the cancer is a breast cancer. In some embodiments, the cancer is a colorectal cancer. In some embodiments, the cancer is a schwannoma. In some embodiments, the schwannoma is a bilateral vestibular schwannoma.

Also provided herein, in some embodiments, are methods for treating a subject suffering from a cancer of the central nervous system comprising administering to the subject a therapeutically effective amount of a compound of Formula I-XV, or a pharmaceutically acceptable salt, solvate, or N-oxide thereof, wherein compounds of Formula I-XV are as described herein.

In some embodiments, the cancer of the central nervous system is a tumor associated with neurofibromatosis type 1 or neurofibromatosis type 2. In some embodiments, the tumor associated with neurofibromatosis type 1 or neurofibromatosis type 2 is a neurofibroma, optic glioma, malignant peripheral nerve sheath tumor, schwannoma, ependymoma, or meningioma. In some embodiments, the schwannoma is a bilateral vestibular schwannoma.

In some embodiments, the cancer is a recurrent cancer. In some embodiments, the cancer is a refractory cancer. In some embodiments, the cancer is a malignant cancer. In some embodiments, is a method for treating a non-malignant tumor.

Also provided herein, in some embodiments, are methods for treating a subject suffering from a cancer of the nervous system comprising administering to the subject a therapeutically effective amount of a compound of Formula I-XV, or a pharmaceutically acceptable salt, solvate, or N-oxide thereof, wherein compounds of Formula I-XV are as described herein.

In some embodiments, the cancer of the nervous system is a tumor of the peripheral nervous system.

In some embodiments, the methods disclosed herein further comprise administering a second therapeutic agent. In some embodiments, the second therapeutic agent is an anti-cancer agent. In some embodiments, the anti-cancer agent is a pro-apoptotic agent or a kinase inhibitor. In some embodiments, the anti-cancer agent is a pro-apoptotic agent, a kinase inhibitor, or a receptor tyrosine kinase inhibitor. In some embodiments, the pro-apoptotic agent is an antagonist of inhibitor of apoptosis (IAP) proteins. In some embodiments, the antagonist of IAP proteins is BV6 or G-416. In some embodiments, the kinase inhibitor is a receptor tyrosine kinase (RTK) inhibitor, non-receptor tyrosine kinase (non-RTK) inhibitor, or a serine/threonine kinase inhibitor. In some embodiments, the kinase inhibitor is a RTK inhibitor selected from a group comprising an EGFR inhibitor, PDGFR inhibitor, FGFR inhibitor, VEGFR inhibitor, and HGFR inhibitor. In some embodiments, the RTK inhibitor is an EGFR inhibitor selected from a group comprising afatinib, lapatinib, neratinib, erlotinib, neratinib, vandetanib, and gefitinib. In some embodiments, the RTK inhibitor is an PDGFR inhibitor selected from a group comprising axitinib, pazopanib, sorafenib and MP470. In some embodiments, the RTK inhibitor is an FGFR inhibitor selected from a group comprising ponatinib, AZD4547, PD173074, TKI-258, and SU5402. In some embodiments, the RTK inhibitor is an VEGFR inhibitor selected from a group comprising axitinib, AZD2171, pazopanib, regorafenib, semaxanib, sorafenib, tivozanib, foretinib, and vandetanib. In some embodiments, the RTK inhibitor is an HGFR inhibitor selected from a group comprising PHA-665752, crizotinib, PF-02341066, K252a, SU11274, ARQ197, foretinib, SGX523, and MP470. In some embodiments, the kinase inhibitor is a MAPK inhibitor. In some embodiments, the MAPK inhibitor is a RAF inhibitor, MEK inhibitor, ERK inhibitor, or any combination thereof. In some embodiments, the MAPK inhibitor is selected from a group comprising VX-702, JIP-1(153-163), VX-745, LY2228820, vinorelbine, and BIRB796. In some embodiments, the MAPK inhibitor is an ERK inhibitor selected from a group comprising sorafenib, GDC-0879, and BIX 02189. In some embodiments; the MAPK inhibitor is a MEK inhibitor selected from a group comprising AZD6244, CI-1040, PD0325901, RDEA119, U0126-EtOH, PD98059, AS703026, PD318088, AZD8330, TAK-733, and GSK1120212. In some embodiments, the MAPK inhibitor is a RAF inhibitor selected from a group comprising RAF265, GDC-0879, PLX-4720, regorafenib, PLX4032, SB590885, and ZM336372. In some embodiments, the kinase inhibitor is a PI3K/AKT/mTOR inhibitor selected from a group comprising rapamycin, CCI-779, everolimus, NVP-BEZ235, PI-103, temsirolimus, AZD8055, KU-0063794, PF-04691502, CH132799, RG7422, palomid 529, PP242, XL765, GSK1059615, PKI-587, WAY-600, WYE-687, WYE-125132, and WYE-354.

Also provided herein, in some embodiments, are methods for treating a neurofibromatosis in an individual comprising administering to an individual in need thereof a therapeutically effective amount of a compound of Formula I-XV, or a pharmaceutically acceptable salt, solvate, or N-oxide thereof, wherein compounds of Formula I-XV are as described herein.

In some embodiments, the neurofibromatosis is neurofibromatosis type 1 or neurofibromatosis type 2. In some embodiments, treating the neurofibromatosis comprises alleviating a symptom associated with the neurofibromatosis. In some embodiments, the symptom associated with the neurofibromatosis is a symptom associated with a neurofibromatosis type 1 or neurofibromatosis type 2. In some embodiments, the symptom associated with the neurofibromatosis type 1 comprises impaired cognition. In some embodiments, the symptom associated with the neurofibromatosis type 2 comprises impaired hearing, word recognition, tone recognition, tinitis, balance, eye sight, or morbidity resulting from nerve compression.

Provided herein, in some embodiments, are methods of modulating a p21-activated kinase comprising contacting a p21-activated kinase with a compound of Formula I-XV.

In some embodiments of any of the above methods, compounds of any of Formula I-XV are inhibitors of p21-activated kinase. In some embodiments, compounds of any of Formula I-XV inhibit one or more of PAK1, PAK2, PAK3, PAK4, PAK5 or PAK6. In some embodiments of any of the above methods compounds of any of Formula I-XV inhibit one or more of PAK1, PAK2 or PAK3. In some embodiments of any of the above methods, compounds of any of Formula I-XV inhibit PAK1 and PAK3. In some embodiments of any of the above methods, compounds of any of Formula I-XV inhibit PAK1 and PAK2. In some embodiments of any of the above methods, compounds of any of Formula I-XV inhibit PAK1, PAK2 and PAK3. In some embodiments of any of the above methods, compounds of any of Formula I-XV inhibit PAK1 and PAK4. In some embodiments of any of the above methods, compounds of any of Formula I-XV inhibit PAK1, PAK2, PAK3 and PAK4.

In some embodiments of any of the above methods, compounds of any of Formula I-XV inhibit PAK1. In some embodiments of any of the above methods, compounds of any of Formula I-XV inhibit PAK2. In some embodiments of any of the above methods, compounds of any of Formula I-XV inhibit PAK3. In some embodiments of any of the above methods, compounds of any of Formula I-XV inhibit PAK4.

In some embodiments of any of the above methods, a therapeutically effective amount of compounds of any of Formula I-XV causes substantially complete inhibition of one or more Group I p21-activated kinases.

In some embodiments of any of the above methods, a therapeutically effective amount of compounds of any of Formula I-XV causes partial inhibition of one or more Group I p21-activated kinases.

In one embodiment the CNS disorder is a neurodegenerative disorder, a neurodevelopmental disorder or a neuropsychiatric disorder.

In some embodiments of any of the above methods, the neuropsychiatric disorder is a psychotic disorder, a mood disorder or cognitive impairment.

In some embodiments of any of the above methods, the CNS disorder is Schizophrenia, Psychotic disorder, schizoaffective disorder, schizophreniform, Alzheimer's disease, Age-related cognitive decline, Mild cognitive impairment, cognitive decline associated with menopause, Parkinson's Disease, Huntington's Disease, Substance abuse and substance dependence, Fragile X, Rett's syndrome, Angelman Syndrome, Asperger's Syndrome, Autism, Autism Spectrum Disorders, Neurofibromatosis I, Neurofibromatosis II, Tuberous sclerosis, Clinical Depression, Bipolar Disorder, Mania, Epilepsy, Mental retardation, Down's syndrome, Niemann-Pick disease, Spongiform encephalitis, Lafora disease, Maple syrup urine disease, maternal phenylketonuria, atypical phenylketonuria, Generalized Anxiety Disorder, Lowe Syndrome, Turner Syndrome, Obsessive-compulsive disorder, Panic disorder, Phobias, Posttraumatic Stress Disorder, Anorexia Nervosa, and Bulimia Nervosa.

In some embodiments of any of the above methods, compounds of any of Formula I-XV modulate dendritic spine morphology or synaptic function. In some embodiments of any of the above methods, compounds of any of Formula I-XV modulate dendritic spine density. In some embodiments of any of the above methods, compounds of any of Formula I-XV modulate dendritic spine length. In some embodiments of any of the above methods, compounds of any of Formula I-XV modulate dendritic spine neck diameter. In some embodiments of any of the above methods, compounds of any of Formula I-XV modulate dendritic spine head volume. In some embodiments of any of the above methods, compounds of any of Formula I-XV modulate dendritic spine head diameter. In some embodiments of any of the above methods, compounds of any of Formula I-XV modulate the ratio of the number of mature spines to the number of immature spines. In some embodiments of any of the above methods, compounds of any of Formula I-XV modulate the ratio of the spine head diameter to spine length. In some embodiments of any of the above methods, compounds of any of Formula I-XV modulate synaptic function.

In some embodiments of any of the above methods, compounds of any of Formula I-XV normalize or partially normalize aberrant baseline synaptic transmission associated with a CNS disorder. In some embodiments of any of the above methods, compounds of any of Formula I-XV normalize or partially normalize aberrant synaptic plasticity associated with a CNS disorder. In some embodiments of any of the above methods, compounds of any of Formula I-XV normalize or partially normalize aberrant long term depression (LTD) associated with a CNS disorder. In some embodiments of any of the above methods, compounds of any of Formula I-XV normalize or partially normalize aberrant long term potentiation (LTP) associated with a CNS disorder.

In some embodiments of any of the above methods, compounds of any of Formula I-XV normalize or partially normalize aberrant sensorimotor gating associated with a CNS disorder such as a neuropsychiatric disorder. In some embodiments of any of the above methods, compounds of any of Formula I-XV reduce or reverse negative symptoms associated with a CNS disorder. In some of such embodiments, the negative symptoms associated with a CNS disorder are asociality, blunted affect, avolition, alogia, anhedonia or dysphoric mood. In some embodiments of any of the above methods, compounds of any of Formula I-XV reduce or reverse positive symptoms associated with a CNS disorder. In some of such embodiments, the positive symptoms associated with a CNS disorder are auditory, visual or tactile hallucinations.

In some embodiments of any of the above methods, compounds of any of Formula I-XV reduce or reverse cognitive symptoms associated with a CNS disorder. In some of such embodiments, the cognitive symptoms associated with a CNS disorder are impairment in executive function, comprehension, inference, decision-making, planning, learning or memory.

In some embodiments of any of the above methods compounds of any of Formula I-XV halt or delay progression of cognitive impairment associated with a CNS disorder. In some of such embodiments, the cognitive impairment is mild cognitive impairment. In some embodiments, the cognitive impairment is associated with Alzheimer's disease.

In some embodiments of any of the above methods, compounds of any of Formula I-XV reduce or reverse behavioral symptoms associated with a CNS disorder. In some of such embodiments, behavioral symptoms include, for example, repetitive behavior (stereotypy), hypersensitivity, hyperactivity, impaired social interaction, autism or the like.

In some embodiments of any of the above methods, the method further comprises administration of a second therapeutic agent that alleviates one or more symptoms associated with a CNS disorder.

In some embodiments, the second therapeutic agent is an antipsychotic agent, a cognition enhancer, a Group I mGluR antagonist, a mGluR5 antagonist, a mGluR5 potentiator, a nootropic agent, an alpha7 nicotinic receptor agonist, an allosteric alpha7 nicotinic receptor potentiator, a nootropic agent, a trophic agent, an antioxidant, a neuroprotectant, a beta secretase inhibitor, a gamma secretase inhibitor or an Abeta antibody.

In some embodiments, administration of a therapeutically effect amount of compounds of any of Formula I-XV to an individual in need thereof improves one or more of MATRICS cognition scores, Wisconsin Card Sort test scores, Mini-Mental State Exam (MMSE) scores, Alzheimer Disease Assessment Scale-Cognitive (ADAS-cog) scale scores, ADAS-Behav scores, or Hopkins Verbal Learning Test Revised scores for the individual.

Provided herein are methods for reversing cortical hypofrontality associated with a CNS disorder comprising administering to an individual in need thereof a therapeutically effective amount of a compound of any of Formula I-XV. Provided herein are methods for reducing, stabilizing, or reversing neuronal withering and/or loss of synaptic function associated a CNS disorder comprising administering to an individual in need thereof a therapeutically effective amount of a compound of any of Formula I-XV. Provided herein are methods for reducing, stabilizing or reversing atrophy or degeneration of nervous tissue in the brain associated with a CNS disorder comprising administering to an individual in need thereof a therapeutically effective amount of a compound of any of Formula I-XV.

Provided herein are methods of inhibiting the activity of one or more p21-activated kinases comprising contacting the one or more p21-activated kinases with a compound of any of Formula I-XV. In some embodiments, the one or more p21-activated kinase is contacted with a compound of any of Formula I-XV in vitro. In some embodiments, the one or more p21-activated kinase is contacted with a compound of any of Formula I-XV in vivo.

Provided herein is the use of compounds of any of Formula I-XV in the manufacture of a medicament for the treatment of a CNS disorder.

As used herein, compounds of any of Formula I-XV includes compounds of Formula I, compounds of Formula II, compounds of Formula III, compounds of Formula IV, compounds of Formula V, compounds of Formula VI, compounds of Formula VII, compounds of Formula VIII, compounds of Formula IX, compounds of Formula X, compounds of Formula XI, compounds of Formula XII, compounds of Formula XIII, compounds of Formula XIV, or compounds of Formula XV.

BRIEF DESCRIPTION OF THE DRAWINGS

The features of the present disclosure are set forth with particularity in the appended claims. A better understanding of the features and advantages of the present invention will be obtained by reference to the following detailed description that sets forth illustrative embodiments, in which the principles of the invention are utilized, and the accompanying drawings of which:

FIG. 1 describes illustrative shapes of dendritic spines.

FIG. 2 describes modulation of dendritic spine head diameter by a small molecule PAK inhibitor.

FIG. 3 describes modulation of dendritic spine length by a small molecule PAK inhibitor.

FIG. 4 describes tumor growth inhibition in a NF2 deficient model by a small molecule PAK inhibitor.

FIG. 5 describes tumor growth inhibition in an orthotopic NF2 mouse model by a small molecule PAK inhibitor.

FIG. 6 describes modulation of NF2−/− Schwannoma cell proliferation by a small molecule PAK inhibitor.

FIG. 7 describes tumor growth inhibition in NF2^(−/−) mesothelioma cells (NCI-H226) by a small molecule PAK inhibitor.

FIG. 8 describes tumor growth inhibition in a PAK1 amplified NSCLC cell line (EBC-1) by a small molecule PAK inhibitor.

FIG. 9 describes tumor growth inhibition in a PAK1 amplified NSCLC cell line (NCI-H520)

FIG. 10 describes tumor growth inhibition in a PAK1 amplified NSCLC cell line (SK-MES-1) by a small molecule PAK inhibitor.

DETAILED DESCRIPTION OF THE INVENTION

Provided herein are methods for treatment of CNS conditions by administration of inhibitors of certain p21 activated kinases to individuals in need thereof. Such kinase inhibitors are inhibitors of one or more of PAK1, PAK2, PAK3, PAK4, PAK5 or PAK6 kinases. In certain embodiments, the individual has been diagnosed with or is suspected of suffering from a CNS disorder such as a neuropsychiatric and/or neurodegenerative and/or neurodevelopmental disease or condition that is mediated by p21 activated kinases. In some instances, provided herein are methods for treating conditions characterized by abnormal dendritic spine morphology and/or spine density and/or spine length and/or spine thickness comprising inhibiting PAK activity by administration of a therapeutically effective amount of a PAK inhibitor to an individual diagnosed with or suspected of suffering from a CNS disorder (e.g., Schizophrenia, Psychotic disorder, schizoaffective disorder, schizophreniform, Alzheimer's disease, Age-related cognitive decline, Mild cognitive impairment, cognitive decline associated with menopause, Parkinson's Disease, Huntington's Disease, Substance abuse and substance dependence, Fragile X, Rett's syndrome, Angelman Syndrome, Asperger's Syndrome, Autism, Autism Spectrum Disorders, Neurofibromatosis I, Neurofibromatosis II, Tuberous sclerosis, Clinical Depression, Bipolar Disorder, Mania, Epilepsy, Mental retardation, Down's syndrome, Niemann-Pick disease, Spongiform encephalitis, Lafora disease, Maple syrup urine disease, maternal phenylketonuria, atypical phenylketonuria, Generalized Anxiety Disorder, Turner Syndrome, Lowe Syndrome, Obsessive-compulsive disorder, Panic disorder, Phobias, Posttraumatic Stress Disorder, Anorexia Nervosa, and Bulimia Nervosa).

A number of CNS disorders are characterized by abnormal dendritic spine morphology, spine size, spine plasticity and/or spine density as described in a number of studies referred to herein. PAK kinase activity has been implicated in spine morphogenesis, maturation, and maintenance. See, e.g., Kreis et al (2007), J Biol Chem, 282(29):21497-21506; Hayashi et al (2007), Proc Natl Acad Sci USA., 104(27):11489-11494, Hayashi et al (2004), Neuron, 42(5):773-787; Penzes et al (2003), Neuron, 37:263-274. In some embodiments, inhibition or partial inhibition of one or more PAKs normalizes aberrant dendritic spine morphology and/or synaptic function. CNS disorders that are treated by the methods described herein include, but are not limited to, Schizophrenia, Psychotic disorder, schizoaffective disorder, schizophreniform, Alzheimer's disease, Age-related cognitive decline, Mild cognitive impairment, cognitive decline associated with menopause, Parkinson's Disease, Huntington's Disease, Substance abuse and substance dependence, Fragile X, Rett's syndrome, Angelman Syndrome, Asperger's Syndrome, Autism, Autism Spectrum Disorders, Neurofibromatosis I, Neurofibromatosis II, Tuberous sclerosis, Clinical Depression, Bipolar Disorder, Mania, Epilepsy, Mental retardation, Down's syndrome, Niemann-Pick disease, Spongiform encephalitis, Lafora disease, Maple syrup urine disease, maternal phenylketonuria, atypical phenylketonuria, Generalized Anxiety Disorder, Obsessive-compulsive disorder, Panic disorder, Phobias, Posttraumatic Stress Disorder, Anorexia Nervosa, and Bulimia Nervosa.

In some instances, CNS disorders are associated with abnormal dendritic spine morphology, spine size, spine plasticity, spine motility, spine density and/or abnormal synaptic function. In some instances, activation of one or more of PAK1, PAK2, PAK3, PAK4, PAK5 and/or PAK6 kinases is implicated in defective spine morphogenesis, maturation, and maintenance. Described herein are methods for suppressing or reducing PAK activity (e.g., by administering a PAK inhibitor for rescue of defects in spine morphology, size, plasticity spine motility and/or density) associated with CNS disorders as described herein. Accordingly, in some embodiments, the methods described herein are used to treat an individual suffering from a CNS disorder wherein the disease is associated with abnormal dendritic spine density, spine size, spine plasticity, spine morphology, spine plasticity, or spine motility.

In some embodiments, any inhibitor of one or more p21-activated kinases described herein reverses or partially reverses defects in dendritic spine morphology and/or dendritic spine density and/or synaptic function that are associated with a CNS disorder. In some embodiments, modulation of dendritic spine morphology and/or dendritic spine density and/or synaptic function alleviates or reverses cognitive impairment and/or negative behavioral symptoms (e.g., social withdrawal, anhedonia or the like) associated with CNS disorders such as psychiatric conditions. In some embodiments, modulation of dendritic spine morphology and/or dendritic spine density and/or synaptic function halts or delays progression of cognitive impairment and/or loss of bodily functions associated with CNS disorders.

In some instances, cellular changes in brain cells contribute to pathogenesis of a CNS disorder. In some instances, abnormal dendritic spine density in the brain contributes to the pathogenesis of a CNS disorder. In some instances, abnormal dendritic spine morphology contributes to the pathogenesis of a CNS disorder. In some instances, an abnormal pruning of dendritic spines or synapses during puberty contributes to the pathogenesis of a CNS disorder. In some instances, abnormal synaptic function contributes to the pathogenesis of a CNS disorder. In some instances, activation of one or more PAKs is associated with abnormal dendritic spine density and/or dendritic morphology and/or synaptic function and contributes to the pathogenesis of a CNS disorder. In some instances, modulation of PAK activity (e.g., attenuation, inhibition or partial inhibition of PAK activity) reverses or reduces abnormal dendritic spine morphology and/or dendritic spine density and/or synaptic function. In certain embodiments, modulation of activity of one or more Group I PAKs (one or more of PAK1, PAK2 and/or PAK3) reverses or reduces abnormal dendritic spine morphology and/or dendritic spine density and/or synaptic function associated with CNS disorders.

Abnormal dendritic spine morphology and/or density have been found in a number of CNS disorders as described below. Accordingly, in some embodiments, the methods described herein are used to treat an individual suffering from a CNS disorder that is associated with abnormal dendritic spine density, spine size, spine plasticity, spine morphology, or spine motility. In some embodiments, the methods described herein are used to treat an individual suffering from a CNS disorder, such as a psychotic disorder, as described in, by way of example, Example 10 and Example 19 herein. Examples of psychotic disorders include, but are not limited to, schizophrenia, schizoaffective disorder, schizophreniform disorder, brief psychotic disorder, delusional disorder, shared psychotic disorder (Folie a Deux), substance induced psychosis, and psychosis due to a general medical condition. See, e.g., Black et al. (2004), Am J Psychiatry, 161:742-744; Broadbelt et al. (2002), Schizophr Res, 58:75-81; Glantz et al. (2000), Arch Gen Psychiatry 57:65-73; and Kalus et al. (2000), Neuroreport, 11:3621-3625. In some instances, aberrant spine morphogenesis is associated with negative symptoms (e.g., asociality, blunted affect, avolition, alogia, anhedonia or dysphoric mood), and/or cognitive impairment symptomatic of schizophrenia. In some instances, aberrant spine morphogenesis is associated with positive symptoms and behavioral changes (e.g., social withdrawal, depersonalization, loss of appetite, loss of hygiene, delusions, hallucinations, the sense of being controlled by outside forces or the like) symptomatic of schizophrenia.

In some embodiments, the methods described herein are used to treat an individual suffering from a mood disorder. Examples of mood disorders include, but are not limited to, clinical depression as described in, for example, Example 12 herein, bipolar disorder, cyclothymia, and dysthymia. See, e.g., Hajszan et al (2005), Eur J Neurosci, 21:1299-1303; Law et al (2004) Am J Psychiatry, 161(10):1848-1855; Norrholm et al. (2001), Synapse, 42:151-163; and Rosoklija et al. (2000), Arch Gen Psychiatry, 57:349-356.

In some embodiments, the methods described herein are used to treat an individual suffering from neurodegenerative disorders (e.g., Parkinson's disease, Alzheimer's disease (as described in, for example, Example 12 herein) or the like). See, e.g., Dickstein et al (2007), Aging Cell, 6:275-284; and Page et al. (2002), Neuroscience Letters, 317:37-41. In some embodiments, the methods described herein are used to treat an individual suffering from or suspected of having mild cognitive impairment (MCI). In some embodiments, the methods described herein are used to halt or delay progression of mild cognitive impairment (MCI) to early dementia, mid-stage dementia or late stage dementia in an individual suffering from or suspected of having mild cognitive impairment (MCI). In some instances, Alzheimer's disease is associated with abnormal dendritic spine morphology, spine size, spine plasticity, spine motility, spine density and/or abnormal synaptic function. In some instances, soluble Abeta dimers and/or oligomers increase PAK kinase activity at the synapse. In some instances, Abeta plaques and/or insoluble Abeta aggregates increase PAK kinase activity at the synapse. In some instances, increased PAK kinase activity is associated with defective spine morphogenesis, maturation, and maintenance. In some instances, PAK inhibitors reverse defects in synaptic function and plasticity in a patient diagnosed with Alzheimer's disease before Abeta plaques can be detected. In some embodiments, PAK inhibitors reverse defects in synaptic morphology, synaptic transmission and/or synaptic plasticity induced by soluble Abeta dimers and/or oligomers. In some embodiments, PAK inhibitors reverse defects in synaptic morphology, synaptic transmission and/or synaptic plasticity induced by Abeta oligomers and/or Abeta-containing plaques.

In some embodiments, the methods described herein are used to treat an individual suffering from epilepsy as described in, for example, Example 20 herein. See, e.g., Wong (2005), Epilepsy and Behavior, 7:569-577; Swann et al (2000), Hippocampus, 10:617-625; and Jiang et al (1998), J Neurosci, 18(20):8356-8368.

In some embodiments, the methods described herein are used to treat an individual suffering from Parkinson's Disease or Huntington's Disease. See, e.g., Neely et al (2007), Neuroscience, 149(2):457-464; Spires et al (2004), Eur J Neurosci, 19:2799-2807; Klapstein et al (2001), J Neurophysiol, 86:2667-2677; Ferrante et al (1991), J Neurosci, 11:3877-3887; and Graveland et al (1985), Science, 227:770-773.

In some embodiments, the methods described herein are used to treat an individual suffering from mental retardation, Fragile X syndrome, autism spectrum disorders or the like. Examples for Autism spectrum Disorders include, but are not limited to, Rett's syndrome, Angelman Syndrome, Asperger's Syndrome, Fragile X syndrome or Tuberous sclerosis.

In some embodiments, the methods described herein are used to treat an individual suffering from mental retardation. Mental retardation is a disorder characterized by significantly impaired cognitive function and deficits in adaptive behaviors. Mental retardation is often defined as an Intelligence Quotient (IQ) score of less than 70. In some instances, mental retardation is Down's syndrome, Fetal alcohol syndrome, Klinefelter's syndrome, congenital hypothyroidism, Williams syndrome, Smith-Lemli-Opitz syndrome, Prader-Willi syndrome Phelan-McDermid syndrome, Mowat-Wilson syndrome, ciliopathy or Lowe syndrome.

In some embodiments, the methods described herein are used to treat an individual suffering from neurofibromatosis. Neurofibromatosis (NF), also called von Recklinghaus disease, is an autosomal dominant genetically-inherited disorder in which the nerve tissue grows tumors (i.e., neurofibromas, ocular gliomas or the like). Patients with NF1 exhibit a number of different disease symptoms including increased risk of forming nervous system tumors and cognitive deficits such as defects in visual-spatial function, attention and motor coordination.

As used herein, NF includes Type 1 NF and Type 2 NF. In some instances, Type 1 NF is inherited or results from spontaneous mutation of neurofibromin. In some instances, NF Type 1 is associated with learning disabilities in individuals affected by the disease. In some instances the disease is associated with a partial absence seizure disorder. In some instances NF Type 1 is associated with poor language, visual-spatial skills, learning disability (e.g., attention deficit hyperactivity disorder), headache, epilepsy or the like.

Type 2 NF is inherited or results from spontaneous mutation of merlin. In some instances, NF Type 2 causes symptoms of hearing loss, tinnitus, headaches, epilepsy, cataracts and/or retinal abnormalities, paralysis and/or learning disabilities. Patients with NF1 and NF2 are at increased risk of forming nervous system tumors. In type 1 patients this includes dermal and plexiform neurofibromas, malignant peripheral nerve sheath tumors (MPNST) and other malignant tumors, while type 2 patients may develop multiple cranial and spinal tumors.

In some instances, developmental disability and/or behavioral problems associated with NF are associated with an abnormality in dendritic spine morphology and/or an abnormality in dendritic spine density and/or an abnormality in synaptic function. In some instances, an abnormality in dendritic spine morphology and/or dendritic spine density and/or synaptic function is associated with activation of p21-activated kinase (PAK). In some instances, modulation of PAK activity (e.g., inhibition or partial inhibition of PAK) alleviates, reverses or reduces abnormalities in dendritic spine morphology and/or dendritic spine density and/or synaptic function thereby reversing or partially reversing developmental disability and/or behavioral problems associated with NF. In some instances, modulation of PAK activity (e.g., inhibition or partial inhibition of PAK) alleviates, reverses or reduces abnormalities in dendritic spine morphology and/or dendritic spine density and/or synaptic function thereby reducing occurrence of seizures in individuals diagnosed with NF. In some instances, modulation of PAK activity (e.g., inhibition or partial inhibition of PAK) alleviates, reverses or reduces abnormalities in dendritic spine morphology and/or dendritic spine density and/or synaptic function thereby reducing or reversing learning disabilities associated with NF. In some instances, modulation of PAK activity (e.g., inhibition or partial inhibition of PAK) alleviates, reverses or reduces cognitive deficits associated with NF. In some instances, modulation of PAK activity (e.g., inhibition or partial inhibition of PAK) alleviates, reverses or reduces learning disability and/or epilepsy and/or any other symptoms associated with NF. In some instances, modulation of PAK activity (e.g., inhibition or partial inhibition of PAK) alleviates, reverses or reduces the incidence of tumor development associated with NF.

In some embodiments, the methods described herein are used to treat an individual suffering from Epilepsy, Niemann-Pick disease, spongiform encephalitis, Lafora disease, Maple syrup urine disease, maternal phenylketonuria, atypical phenylketonuria, age-related cognitive decline and cognitive decline associated with menopause.

In some instances, development of a CNS disorder is associated with a genetic component. Certain risk alleles and genes that have been identified for CNS disorders. For example, for Alzheimer's disease, risk alleles and genes include mutations in Amyloid Precursor Protein (APP), mutations in presenilin 1 and 2, the epsilon4 allele, the 91 bp allele in the telomeric region of 12q, Apolipoprotein E-4 (APOE4) gene, SORL1 gene, reelin gene or the like. For example, in some instances, development of schizophrenia is associated with mutations in the DISC1 gene. In some instances, several risk alleles or genes are involved in etiology of a CNS disorder. In some instances, CNS disorders run in families and there is a predisposition or vulnerability to the illness. In some instances, a combination of genetic, familial and environmental factors play a role in manifestation of disease symptoms. In some instances, mutations in genes resulting in a predisposition to a CNS disorders leads to early-onset of the disease.

Dendritic Spines

A dendritic spine is a small membranous protrusion from a neuron's dendrite that serves as a specialized structure for the formation, maintenance, and/or function of synapses. Dendritic spines vary in size and shape. In some instances, spines have a bulbous head (the spine head) of varying shape, and a thin neck that connects the head of the spine to the shaft of the dendrite. In some instances, spine numbers and shape are regulated by physiological and pathological events. In some instances, a dendritic spine head is a site of synaptic contact. In some instances, a dendritic spine shaft is a site of synaptic contact. FIG. 1 shows examples of different shapes of dendritic spines. Dendritic spines are “plastic.” In other words, spines are dynamic and continually change in shape, volume, and number in a highly regulated process. In some instances, spines change in shape, volume, length, thickness or number in a few hours. In some instances, spines change in shape, volume, length, thickness or number occurs within a few minutes. In some instances, spines change in shape, volume, length, thickness or number occurs in response to synaptic transmission and/or induction of synaptic plasticity. By way of example, dendritic spines are headless (filopodia as shown, for example, in FIG. 1 a), thin (for example, as shown in FIG. 1 b), stubby (for example as shown in FIG. 1 c), mushroom-shaped (have door-knob heads with thick necks, for example as shown in FIG. 1 d), ellipsoid (have prolate spheroid heads with thin necks, for example as shown in FIG. 1 e), flattened (flattened heads with thin neck, for example as shown in FIG. 1 f) or branched (for example as shown in FIG. 1 g).

In some instances, mature spines have variably-shaped bulbous tips or heads, ˜0.5-2 μm in diameter, connected to a parent dendrite by thin stalks 0.1-1 μm long. In some instances, an immature dendritic spine is filopodia-like, with a length of 1.5-4 μm and no detectable spine head. In some instances, spine density ranges from 1 to 10 spines per micrometer length of dendrite, and varies with maturational stage of the spine and/or the neuronal cell. In some instances, dendritic spine density ranges from 1 to 40 spines per 10 micrometer in medium spiny neurons.

In some instances, the shape of the dendritic spine head determines synpatic function. Defects in dendritic spine morphology and/or function have been described in neurological diseases. As an example only, the density of dendritic spines has been shown to be reduced in pyramidal neurons from patients with schizophrenia (Glanz and Lewis, Arch Gen Psychiatry, 2000:57:65-73). In another example, neurons from patients with Fragile X mental retardation show a significant increase in the overall density of dendritic spines, together with an increase in the proportion of“immature”, filopodia-like spines and a corresponding reduction of “mature”, mushrooms-shaped spines (Irvin et al, Cerebral Cortex, 2000; 10:1038-1044). In many cases, the dendritic spine defects found in samples from human brains have been recapitulated in rodent models of the disease and correlated to defective synapse function and/or plasticity. In some instances, dendritic spines with larger spine head diameter form more stable synapses compared with dendritic spines with smaller head diameter. In some instances, a mushroom-shaped spine head is associated with normal or partially normal synaptic function. In some instances, a mushroom-shaped spine is a healthier spine (e.g., having normal or partially normal synapses) compared to a spine with a reduced spine head size, spine head volume and/or spine head diameter. In some instances, inhibition or partial inhibition of PAK activity results in an increase in spine head diameter and/or spine head volume and/or reduction of spine length, thereby normalizing or partially normalizing synaptic function in individuals suffering or suspected of suffering from a CNS disorder.

Cell-Proliferative Disorders

In some embodiments, the compounds and formulations described herein are utilized to treat one or more diseases, or disorders characterized by aberrant cell proliferation. In some embodiments, the disease or disorder characterized by aberrant cell proliferation is a cancer. In some embodiments, the cancer is a malignant cancer. In some embodiments, the cancer is a solid tumor. In some embodiments, the solid tumor is a sarcoma or carcinoma. In some embodiments, the cancer is a leukemia or lymphoma. In some embodiments, the cancer is a recurrent cancer. In some embodiments, the cancer is a refractory cancer.

A cancer is an abnormal growth of cells (usually derived from a single cell). The cells have lost normal control mechanisms and thus are able to expand continuously, invade adjacent tissues, migrate to distant parts of the body, and promote the growth of new blood vessels from which the cells derive nutrients. In some embodiments, the cancer is a malignant cancer. Cancer can develop from any tissue within the body. As cells grow and multiply, they form a mass of tissue, called a tumor. The term tumor refers to an abnormal growth or mass. Tumors can be cancerous (malignant) or noncancerous (benign). Cancerous tumors can invade neighboring tissues and spread throughout the body (metastasize). Benign tumors, however, do not invade neighboring tissues and do not spread throughout the body. In some embodiments, the cancer is a malignant cancer. In some embodiments, the tumor is a non-malignant tumor. Cancer can be divided into those of the blood and blood-forming tissues (leukemias and lymphomas) and “solid” tumors. “Solid” tumors can be carcinomas or sarcomas.

In some embodiments, the cancer is a leukemia or a lymphoma. In some embodiments, the cancer is a leukemia. Leukemias are cancers of white blood cells or of cells that develop into white blood cells. White blood cells develop from stem cells in the bone marrow. Sometimes the development goes awry, and pieces of chromosomes get rearranged. The resulting abnormal chromosomes interfere with normal control of cell division, so that affected cells multiply uncontrollably and become cancerous (malignant), resulting in leukemia. Leukemia cells ultimately occupy the bone marrow, replacing or suppressing the function of cells that develop into normal blood cells. This interference with normal bone marrow cell function can lead to inadequate numbers of red blood cells (causing anemia), white blood cells (increasing the risk of infection), and platelets (increasing the risk of bleeding). Leukemia cells may also invade other organs, including the liver, spleen, lymph nodes, testes, and brain. Leukemias are grouped into four main types: acute lymphocytic leukemia, acute myelocytic leukemia, chronic lymphocytic leukemia, chronic myelocytic leukemia. The types are defined according to how quickly they progress and the type and characteristics of the white blood cells that become cancerous. Acute leukemias progress rapidly and consist of immature cells. Chronic leukemias progress slowly and consist of more mature cells. Lymphocytic leukemias develop from cancerous changes in lymphocytes or in cells that normally produce lymphocytes. Myelocytic (myeloid) leukemias develop from cancerous changes in cells that normally produce neutrophils, basophils, eosinophils, and monocytes. Additional types of leukemias include hairy cell leukemia, chronic myelomonocytic leukemia, and juvenile myelomonocytic-leukemia.

In some embodiments, the cancer is a lymphoma. Lymphomas are cancers of the lymphocytes, which reside in the lymphatic system and in blood-forming organs. Lymphomas are cancers of a specific type of white blood cell known as lymphocytes. These cells help fight infections. Lymphomas can develop from either B or T lymphocytes. T lymphocytes are important in regulating the immune system and in fighting viral infections. B lymphocytes produce antibodies. Lymphocytes move about to all parts of the body through the bloodstream and through a network of tubular channels called lymphatic vessels. Scattered throughout the network of lymphatic vessels are lymph nodes, which house collections of lymphocytes. Lymphocytes that become cancerous (lymphoma cells) may remain confined to a single lymph node or may spread to the bone marrow, the spleen, or virtually any other organ. The two major types of lymphoma are Hodgkin lymphoma, previously known as Hodgkin's disease, and non-Hodgkin lymphoma. Non-Hodgkin lymphomas are more common than Hodgkin lymphoma. Burkitt's lymphoma and mycosis fungoides are subtypes of non-Hodgkin lymphomas. Hodgkin lymphoma is marked by the presence of the Reed-Sternberg cell. Non-Hodgkin lymphomas are all lymphomas which are not Hodgkin's lymphoma. Non-Hodgkin lymphomas can be further divided into indolent lymphomas and aggressive lymphomas. Non-Hodgkin's lymphomas include, but are not limited to, diffuse large B cell lymphoma, follicular lymphoma, mucosa-associated lymphatic tissue lymphoma (MALT), small cell lymphocytic lymphoma, mantle cell lymphoma, Burkitt's lymphoma, mediastinal large B cell lymphoma, Waldenström macroglobulinemia, nodal marginal zone B cell lymphoma (NMZL), splenic marginal zone lymphoma (SMZL), extranodal marginal zone B cell lymphoma, intravascular large B cell lymphoma, primary effusion lymphoma, and lymphomatoid granulomatosis.

In some embodiments, the cancer is a solid tumor. In some embodiments, the solid tumor is a sarcoma or carcinoma. In some embodiments, the solid tumor is a sarcoma. Sarcomas are cancers of the bone, cartilage, fat, muscle, blood vessels, or other connective or supportive tissue. Sarcomas include, but are not limited to, bone cancer, fibrosarcoma, chondrosarcoma, Ewing's sarcoma, malignant hemangioendothelioma, malignant schwannoma, osteosarcoma, soft tissue sarcomas (e.g. alveolar soft part sarcoma, angiosarcoma, cystosarcoma phylloides, dermatofibrosarcoma, desmoid tumor, epithelioid sarcoma, extraskeletal osteosarcoma, fibrosarcoma, hemangiopericytoma, hemangiosarcoma, Kaposi's sarcoma, leiomyosarcoma, liposarcoma, lymphangiosarcoma, lymphosarcoma, malignant fibrous histiocytoma, neurofibrosarcoma, rhabdomyosarcoma, and synovial sarcoma). In some embodiments, the cancer is a schwannoma. In some embodiments, the schwannoma is a spontaneous schwannoma. In some embodiments, the schwannoma is a malignant scwhannoma. In some embodiments, the schwannoma is a bilateral vestibular scwhannoma.

In some embodiments, the solid tumor is a carcinoma. Carcinomas are cancers that begin in the epithelial cells, which are cells that cover the surface of the body, produce hormones, and make up glands. By way of non-limiting example, carcinomas include breast cancer, pancreatic cancer, lung cancer, colon cancer, colorectal cancer, rectal cancer, kidney cancer, bladder cancer, stomach cancer, prostate cancer, liver cancer, ovarian cancer, brain cancer, vaginal cancer, vulvar cancer, uterine cancer, oral cancer, penic cancer, testicular cancer, esophageal cancer, skin cancer, cancer of the fallopian tubes, head and neck cancer, gastrointestinal stromal cancer, adenocarcinoma, cutaneous or intraocular melanoma, cancer of the anal region, cancer of the small intestine, cancer of the endocrine system, cancer of the thyroid gland, cancer of the parathyroid gland, cancer of the adrenal gland, cancer of the urethra, cancer of the renal pelvis, cancer of the ureter, cancer of the endometrium, cancer of the cervix, cancer of the pituitary gland, neoplasms of the central nervous system (CNS), primary CNS lymphoma, brain stem glioma, and spinal axis tumors. In some embodiments, the cancer is a breast cancer. In some embodiments, the cancer is an ovarian cancer. In some embodiments, the cancer is a head and neck cancer. In some embodiments, the cancer is an esophageal cancer. In some embodiments, the cancer is an esophageal squamous cancer.

In some embodiments, the cancer is a skin cancer. In some embodiments, the skin cancer is a basal cell carcinoma. Basal cell carcinomas account for about more than 90% of all skin cancers. Basal cell carcinomas are generally slow-growing and seldom spread. In some instances, basal cell carcinomas can spread and invade bone and other tissues under the skin. In some embodiments, the skin cancer is a squamous cell carcinoma. Squamous cell carcinomas can be more aggressive than basal cell carcinomas. In some instances, squamous cell carcinomas are more likely to grow deep below the skin and spread to distant parts of the body. These types of skin cancer sometimes are called nonmelanoma skin cancer. In some embodiments, the skin cancer is an actinic (solar) keratosis. An actinic keratosis is a precancerous condition that can develop into squamous cell carcinoma. In some instances, actinic keratosis appears as rough, red or brown, scaly patches on the skin. In some instances, they are often more easily felt than seen. In some instances, actinic keratosis is found on sun-exposed areas of the body, but it can be found on other parts of the body as well. In some instances, the skin cancer is a melanoma. A melanoma is a cancer that begins in the cells that produce skin pigment.

In some embodiments, the cancer is a lung cancer. Lung cancer can start in the airways that branch off the trachea to supply the lungs (bronchi) or the small air sacs of the lung (the alveoli). Lung cancers include non-small cell lung carcinoma (NSCLC), small cell lung carcinoma, and mesotheliomia. In some embodiments, the cancer is a NSCLC. NSCLC account for about 85 to 87% of lung cancers. In some instances, NSCLC grows more slowly than small cell lung carcinoma. Nevertheless, in some instances, by the time about 40% of people are diagnosed, the cancer has spread to other parts of the body outside of the chest. Examples of NSCLC include squamous cell carcinoma, adenocarcinoma, and large cell carcinoma. In some instances, the cancer is a small cell lung carcinoma (SCLC). Small cell lung carcinoma, also called oat cell carcinoma, accounts for about 13 to 15% of all lung cancers. In some instances, SCLC is very aggressive and spreads quickly. In some instances, by the time that most people are diagnosed, the cancer has metastasized to other parts of the body. In some embodiments, the cancer is a mesothelioma. In some embodiments, the mesothelioma is a malignant mesothelioma. In some instances, the malignant mesothelioma is an uncommon cancerous tumor of the lining of the lung and chest cavity (pleura) or lining of the abdomen (peritoneum) that is typically due to long-term asbestos exposure.

In some embodiments, the cancer is a CNS tumor. CNS tumors may be classified as gliomas or nongliomas. In some embodiments, the cancer is a glioma. In some instances, the glioma is a malignant glioma. In some embodiments, the glioma is a high grade glioma. In some embodiments, the glioma is a diffuse intrinsic pontine glioma. In some embodiments, the cancer is a nonglioma. Nongliomas include meningiomas, pituitary adenomas, primary CNS lymphomas, and medulloblastomas. In some embodiments, the cancer is a meningioma.

In some embodiments, the cancer is a brain cancer. In some embodiments, the brain cancer is a glioblastoma.

In some instances, the cancer is a glioma. Examples of gliomas include astrocytomas, oligodendrogliomas (or mixtures of oligodendroglioma and astocytoma elements), and ependymomas. In some embodiments, the cancer is an astrocytoma. Astrocytomas include, but are not limited to, low-grade astrocytomas, anaplastic astrocytomas, glioblastoma multiforme, pilocytic astrocytoma, pleomorphic xanthoastrocytoma, and subependymal giant cell astrocytoma. Glioblastoma multiforme is the most common and most malignant of the primary brain tumors. Although this tumor can occur in all age groups, including children, the average age at which it is diagnosed is 55 years. The onset of symptoms is often abrupt and is most commonly related to mass effect and focal neurologic symptoms. Seizures are also relatively common. Intracranial bleeding may be the presenting symptom in less than 3% of patients. The duration of symptoms before diagnosis is usually short, ranging from a few days to a few weeks.

In some embodiments, the cancer is an oligodendroglioma. Oligodendrogliomas include low-grade oligodendrogliomas (or oligoastrocytomas) and anaplastic oligodendriogliomas.

In some embodiments, the cancer of the CNS is a tumor associated with neurofibromatosis (NF). In some embodiments, the neurofibromatosis is a type 1 NF or a type 2 NF. In some embodiments, the neurofibromatosis is a type 1 NF. Neurofibromatosis type 1 is a condition characterized by changes in skin coloring (pigmentation) and the growth of tumors along nerves in the skin, brain, and other parts of the body. The signs and symptoms of this condition vary widely among affected people.

Beginning in early childhood, almost all people with neurofibromatosis type 1 have multiple café-au-lait spots, which are flat patches on the skin that are darker than the surrounding area. These spots increase in size and number as the individual grows older. Freckles in the underarms and groin typically develop later in childhood.

Most adults with neurofibromatosis type 1 develop neurofibromas, which are noncancerous (benign) tumors that are usually located on or just under the skin. These tumors may also occur in nerves near the spinal cord or along nerves elsewhere in the body. Some people with neurofibromatosis type 1 develop cancerous tumors that grow along nerves. These tumors, which usually develop in adolescence or adulthood, are called malignant peripheral nerve sheath tumors. People with neurofibromatosis type 1 also have an increased risk of developing other cancers, including brain tumors and cancer of blood-forming tissue (leukemia). In some embodiments, the cancer is a neurofibroma.

During childhood, benign growths called Lisch nodules often appear in the colored part of the eye (the iris). Lisch nodules do not interfere with vision. Some affected individuals also develop tumors that grow along the nerve leading from the eye to the brain (the optic nerve). These tumors, which are called optic gliomas, may lead to reduced vision or total vision loss. In some cases, optic gliomas have no effect on vision. In some embodiments, the cancer is an optic glioma.

In some embodiments, the cancer of the CNS is a tumor associated with neurofibromatosis. In some embodiments, the neurofibromatosis is a type 2 NF. Neurofibromatosis type 2 is a disorder characterized by the growth of noncancerous tumors in the nervous system. The tumors associated with neurofibromatosis type 2 are called bilateral vestibular schwannomas, acoustic neuromas, ependyomomas, or meningiomas. These growths develop in the brain or along the nerve that carries information from the inner ear to the brain (the auditory nerve). In some embodiments, the cancer is bilateral vestibular schwannoma, acoustic neuroma, ependyomoma, or meningioma.

The signs and symptoms of this condition usually appear during adolescence or in a person's early twenties, although onset can occur at any age. The most frequent early symptoms of vestibular schwannomas are hearing loss, ringing in the ears (tinnitus), and problems with balance. In most cases, these tumors occur in both ears by age 30. If tumors develop in other parts of the brain or spinal cord, signs and symptoms vary according to their location. Complications of tumor growth can include changes in vision or sensation, numbness or weakness in the arms or legs, fluid buildup in the brain, and nerve compression leading to significant morbidities and death. Some people with neurofibromatosis type 2 also develop clouding of the lens (cataracts) in one or both eyes, often beginning in childhood.

In some embodiments, the cancer is characterized by aberrant NF1 gene expression or activity. In some embodiments, the cancer is characterized by a reduction in NF1 gene expression or activity. In some embodiments, NF1 gene expression or activity is reduced at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%. In other embodiments, NF1 gene expression or activity is reduced at least about 70%, at least about 75%, at least about 80%, or at least about 85%. Preferably, NF1 gene expression or activity is reduced at least about 90%, at least about 95%, at least about 97%, at least about 98%, or at least about 99%. In some embodiments, the cancer is characterized by a mutation in the NF1 gene.

In some embodiments, any of the cancers disclosed herein are characterized by aberrant NF2 gene expression or activity. In some embodiments, the cancer is characterized by a reduction in NF2 gene expression or activity. In some embodiments, NF2 gene expression or activity is reduced at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%. In other embodiments, NF2 gene expression or activity is reduced at least about 70%, at least about 75%, at least about 80%, or at least about 85%. Preferably, NF2 gene expression or activity is reduced at least about 90%, at least about 95%, at least about 97%, at least about 98%, or at least about 99%. In some embodiments, the cancer is characterized by a mutation in the NF2 gene.

p21-Activated Kinases (PAKs)

The PAKs constitute a family of serine-threonine kinases that is composed of “conventional”, or Group I PAKs, that includes PAK1, PAK2, and PAK3, and “non-conventional”, or Group II PAKs, that includes PAK4, PAK5, and PAK6. See, e.g., Zhao et al. (2005), Biochem J, 386:201-214. These kinases function downstream of the small GTPases Rac and/or Cdc42 to regulate multiple cellular functions, including dendritic morphogenesis and maintenance (see, e.g., Ethell et al (2005), Prog in Neurobiol, 75:161-205; Penzes et al (2003), Neuron, 37:263-274), motility, morphogenesis, angiogenesis, and apoptosis, (see, e.g., Bokoch et al., 2003, Annu. Rev. Biochem., 72:743; and Hofmann et al., 2004, J. Cell Sci., 117:4343;). GTP-bound Rac and/or Cdc42 bind to inactive PAK, releasing steric constraints imposed by a PAK autoinhibitory domain and/or permitting PAK phosphorylation and/or activation. Numerous phosphorylation sites have been identified that serve as markers for activated PAK.

In some instances, upstream effectors of PAK include, but are not limited to, TrkB receptors; NMDA receptors; adenosine receptors; estrogen receptors; integrins, EphB receptors; CDK5, FMRP; Rho-family GTPases, including Cdc42, Rac (including but not limited to Rac1 and Rac2), Chp, TC10, and Wrnch-1; guanine nucleotide exchange factors (“GEFs”), such as but not limited to GEFT, α-p-21-activated kinase interacting exchange factor (αPIX), Kalirin-7, and Tiam1; G protein-coupled receptor kinase-interacting protein 1 (GIT1), and sphingosine.

In some instances, downstream effectors of PAK include, but are not limited to, substrates of PAK kinase, such as Myosin light chain kinase (MLCK), regulatory Myosin light chain (R-MLC), Myosins I heavy chain, myosin II heavy chain, Myosin VI, Caldesmon, Desmin, Op18/stathmin, Merlin, Filamin A, LIM kinase (LIMK), Ras, Raf, Mek, p47phox, BAD, caspase 3, estrogen and/or progesterone receptors, RhoGEF, GEF-H1, NET1, Gαz, phosphoglycerate mutase-B, RhoGDI, prolactin, p41 Arc, cortactin and/or Aurora-A (See, e.g., Bokoch et al., 2003, Annu. Rev. Biochem., 72:743; and Hofmann et al., 2004, J. Cell Sci., 117:4343). Other substances that bind to PAK in cells include CIB; sphingolipids; lysophosphatidic acid, G-protein β and/or γ subunits; PIX/COOL; GIT/PKL; Nef; Paxillin; NESH; SH3-containing proteins (e.g. Nck and/or Grb2); kinases (e.g. Akt, PDK1, PI 3-kinase/p85, CdkS, Cdc2, Src kinases, Abl, and/or protein kinase A (PKA)); and/or phosphatases (e.g. phosphatase PP2A, POPX1, and/or POPX2).

PAK Inhibitors

Described herein are PAK inhibitors that treat one or more symptoms associated with CNS disorders. Also described herein are pharmaceutical compositions comprising a PAK inhibitor (e.g., a PAK inhibitor compound described herein) for reversing or reducing one or more of cognitive impairment and/or dementia and/or negative symptoms and/or positive symptoms associated with CNS disorders. Also described herein are pharmaceutical compositions comprising a PAK inhibitor (e.g., a PAK inhibitor compound described herein) for halting or delaying the progression of cognitive impairment and/or dementia and/or negative symptoms and/or positive symptoms associated with CNS disorders. Described herein is the use of a PAK inhibitor for manufacture of a medicament for treatment of one or more symptoms of a CNS disorder.

In some embodiments, the PAK inhibitor is a Group I PAK inhibitor that inhibits, for example, one or more Group I PAK polypeptides, for example, PAK1, PAK2, and/or PAK3. In some embodiments, the PAK inhibitor is a PAK1 inhibitor. In some embodiments, the PAK inhibitor is a PAK2 inhibitor. In some embodiments, the PAK inhibitor is a PAK3 inhibitor. In some embodiments, the PAK inhibitor is a mixed PAK1/PAK3 inhibitor. In some embodiments, the PAK inhibitor is a mixed PAK1/PAK2 inhibitor. In some embodiments, the PAK inhibitor is a mixed PAK1/PAK4 inhibitor. In some embodiments, the PAK inhibitor is a mixed PAK1/PAK2/PAK4 inhibitor. In some embodiments, the PAK inhibitor is a mixed PAK1/PAK2/PAK3/PAK4 inhibitor. In some embodiments, the PAK inhibitor inhibits all three Group I PAK isoforms (PAK1, 2 and PAK3) with equal or similar potency. In some embodiments, the PAK inhibitor is a Group II PAK inhibitor that inhibits one or more Group II PAK polypeptides, for example PAK4, PAK5, and/or PAK6. In some embodiments, the PAK inhibitor is a PAK4 inhibitor. In some embodiments, the PAK inhibitor is a PAK5 inhibitor. In some embodiments, the PAK inhibitor is a PAK6 inhibitor.

In certain embodiments, a PAK inhibitor described herein reduces or inhibits the activity of one or more of PAK1, PAK2, PAK3, and/or PAK4 while not affecting the activity of PAK5 and PAK6. In some embodiments, a PAK inhibitor described herein reduces or inhibits the activity of one or more of PAK1, PAK2 and/or PAK3 while not affecting the activity of PAK4, PAK5 and/or PAK6. In some embodiments, a PAK inhibitor described herein reduces or inhibits the activity of one or more of PAK1, PAK2, PAK3, and/or one or more of PAK4, PAK5 and/or PAK6. In some embodiments, a PAK inhibitor described herein is a substantially complete inhibitor of one or more PAKs. As used herein, “substantially complete inhibition” means, for example, >95% inhibition of one or more targeted PAKs. In other embodiments, “substantially complete inhibition” means, for example, >90% inhibition of one or more targeted PAKs. In some other embodiments, “substantially complete inhibition” means, for example, >80% inhibition of one or more targeted PAKs. In some embodiments, a PAK inhibitor described herein is a partial inhibitor of one or more PAKs. As used herein, “partial inhibition” means, for example, between about 40% to about 60% inhibition of one or more targeted PAKs. In other embodiments, “partial inhibition” means, for example, between about 50% to about 70% inhibition of one or more targeted PAKs. As used herein, where a PAK inhibitor substantially inhibits or partially inhibits the activity of a certain PAK isoform while not affecting the activity of another isoform, it means, for example, less than about 10% inhibition of the non-affected isoform when the isoform is contacted with the same concentration of the PAK inhibitor as the other substantially inhibited or partially inhibited isoforms. In other instances, where a PAK inhibitor substantially inhibits or partially inhibits the activity of a certain PAK isoform while not affecting the activity of another isoform, it means, for example, less than about 5% inhibition of the non-affected isoform when the isoform is contacted with the same concentration of the PAK inhibitor as the other substantially inhibited or partially inhibited isoforms. In yet other instances, where a PAK inhibitor substantially inhibits or partially inhibits the activity of a certain PAK isoform while not affecting the activity of another isoform, it means, for example, less than about 1% inhibition of the non-affected isoform when the isoform is contacted with the same concentration of the PAK inhibitor as the other substantially-inhibited or partially inhibited isoforms.

Provided herein, in certain embodiments, are compounds having the structure of Formula I or pharmaceutically acceptable salt or N-oxide thereof:

wherein:

wherein ring T is an aryl, or a heteroaryl ring;

-   -   R³ is a substituted or unsubstituted cycloalkyl, a substituted         or unsubstituted heteroaryl attached to ring T via a carbon atom         of R³, or a substituted or unsubstituted heterocycloalkyl         attached to ring T via a carbon atom of R³;     -   Q is a substituted or unsubstituted alkyl, a substituted or         unsubstituted heteroalkyl, a substituted or unsubstituted         heterocycloalkyl, a substituted or unsubstituted cycloalkyl, a         substituted or unsubstituted cycloalkylalkyl, a substituted or         unsubstituted heterocycloalkylalkyl, a substituted or         unsubstituted aryl, a substituted or unsubstituted arylalkyl, a         substituted or unsubstituted heteroaryl, or a substituted or         unsubstituted heteroarylalkyl;     -   each R⁴ is independently halogen, —CN, —NO₂, —OH, —OCF₃, —OCH₂F,         —OCF₂H, —CF₃, —SR⁸, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, a substituted or         unsubstituted alkyl, a substituted or unsubstituted alkoxy, a         substituted or unsubstituted heteroalkyl, a substituted or         unsubstituted cycloalkyl, or a substituted or unsubstituted         heterocycloalkyl;     -   R⁸ is H or R⁹;     -   R⁹ is a substituted or unsubstituted alkyl, a substituted or         unsubstituted cycloalkyl, a substituted or unsubstituted         heterocycloalkyl, a substituted or unsubstituted aryl, or a         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, a substituted or unsubstituted         alkyl, a substituted or unsubstituted cycloalkyl, a substituted         or unsubstituted heterocycloalkyl, a substituted or         unsubstituted aryl, or a substituted or unsubstituted         heteroaryl; or two R¹⁰, together with the atoms to which they         are attached form a heterocycle;     -   ring B is aryl or heteroaryl;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═)OR¹⁰, —NR¹⁰C(═O)OR¹⁰, (═O)N(R¹⁰)₂, —OR¹⁰, a substituted         or unsubstituted alkyl, a substituted or unsubstituted alkoxy, a         substituted or unsubstituted heteroalkyl, a substituted or         unsubstituted cycloalkyl, or a substituted or unsubstituted         heterocycloalkyl; or two R⁵ together with the atoms to which         they are attached form a cycloalkyl group or a heterocycloalkyl         group;     -   r is 0 to 8; and     -   s is 0 to 4.

In one embodiment is a compound of Formula I wherein ring T is an aryl ring. In one embodiment, the aryl ring is a phenyl group. In another embodiment is a compound of Formula I wherein ring T is a heteroaryl ring. In yet another embodiment is a compound of Formula I, wherein ring T is selected from pyrrole, furan, thiophene, pyrazole, imidazole, isoxazole, oxazole, isothiazole, thiazole, 1,2,3-triazole, 1,3,4-triazole, 1-oxa-2,3-diazole, 1-oxa-2,4-diazole, 1-oxa-2,5-diazole, 1-oxa-3,4-diazole, 1-thia-2,3-diazole, 1-thia-2,4-diazole, 1-thia-2,5-diazole, 1-thia-3,4-diazole, tetrazole, pyridine, pyridazine, pyrimidine, and pyrazine. In another embodiment, ring T is thiazole.

In a further embodiment is a compound of Formula I, wherein R³ is a C-linked heterocycloalkyl. In one embodiment, the C-linked heterocycloalkyl is oxetane, azetidine, tetrahydropyran, pyrrolidine, tetrahydrothiophene, piperidine, tetrahydropyran, and morpholine. In a further embodiment, the C-linked heterocycloalkyl is substituted with at least one C₁-C₆alkyl or halogen. In another embodiment, the C₁-C₆alkyl is methyl, ethyl, or n-propyl. In one embodiment is a compound of Formula I, wherein R³ is a substituted or unsubstituted C-linked heteroaryl. In one embodiment, R³ is selected from a C-linked pyrrole, furan, thiophene, pyrazole, imidazole, isoxazole, oxazole, isothiazole, thiazole, 1,2,3-triazole, 1,3,4-triazole, 1-oxa-2,3-diazole, 1-oxa-2,4-diazole, 1-oxa-2,5-diazole, 1-oxa-3,4-diazole, 1-thia-2,3-diazole, 1-thia-2,4-diazole, 1-thia-2,5-diazole, 1-thia-3,4-diazole, tetrazole, pyridine, pyridazine, pyrimidine, and pyrazine. In yet another embodiment, R³ is a C-linked thiazole. In another embodiment, R³ is a C-linked pyrazole. In a further embodiment, R³ is a C-linked oxadiazole. In another embodiment, R³ is a substituted or unsubstituted cycloalkyl. In a further embodiment, cycloalkyl is selected from cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and cycloheptyl. In a further embodiment, R³ is cyclopentyl. In another embodiment, R³ is cyclohexyl.

In yet another embodiment, R³ is a C-linked heteroaryl substituted with at least one group selected from halogen, —CN, —NO₂, —OH, —SR⁸, —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸, —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, —OR¹⁰, a substituted or unsubstituted alkyl, a substituted or unsubstituted alkoxy, a substituted or unsubstituted heteroalkyl, a substituted or unsubstituted cycloalkyl, or a substituted or unsubstituted heterocycloalkyl. In one embodiment, the C-linked heteroaryl is substituted with C₁-C₆alkyl. In another embodiment, C₁-C₆alkyl is methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, or tert-butyl. In a further embodiment, the C-linked heteroaryl is substituted with methyl. In another embodiment, ethyl. In a further embodiment, n-propyl or iso-propyl.

Also disclosed herein is a compound of Formula I wherein R⁴ is independently halogen, —CN, —NO₂, —OH, —OCF₃, —OCH₂F, —OCF₂H, —CF₃, —SR⁸, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁹, —OC(═O)R⁸, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, NR¹⁰C(═O)R¹⁰, —N R¹⁰C(═O)OR¹⁰, and —NR¹⁰C(═O)N(R¹⁰)₂. In a further embodiment, R⁴ is a halogen. In yet another embodiment, R⁴ is selected from F, Cl, Br, or I. In another embodiment, R⁴ is F. In yet another embodiment, R⁴ is a substituted or unsubstituted alkyl, a substituted or unsubstituted alkoxy, a substituted or unsubstituted heteroalkyl, a substituted or unsubstituted cycloalkyl, or a substituted or unsubstituted heterocycloalkyl. In one embodiment, R⁴ is substituted or unsubstituted alkyl selected from methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl or tert-butyl. In another embodiment, R⁴ is OH. In a further embodiment, R⁴ is OCH₃. In yet another embodiment, R⁴ is OCF₃.

In another embodiment, s is 1. In yet another embodiment, s is 0.

In one embodiment, is a compound of Formula I wherein Q is a substituted or unsubstituted alkyl, a substituted or unsubstituted heteroalkyl, a substituted or unsubstituted heterocycloalkyl, a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted cycloalkylalkyl, a substituted or unsubstituted heterocycloalkylalkyl, a substituted or unsubstituted aryl, a substituted or unsubstituted arylalkyl, a substituted or unsubstituted heteroaryl, or a substituted or unsubstituted heteroarylalkyl. In another embodiment, Q is a substituted or unsubstituted alkyl. In a further embodiment, Q is an unsubstituted methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl or tert-butyl. In a further embodiment, Q is ethyl.

In yet another embodiment, is a compound of Formula I, wherein ring B is an aryl ring. In another embodiment, ring B is a substituted or unsubstituted phenyl. In a further embodiment, ring B is a substituted or unsubstituted naphthalene. In a further embodiment, is a compound of Formula I, wherein ring B is a heteroaryl ring selected from pyrrole, furan, thiophene, pyrazole, imidazole, isoxazole, oxazole, isothiazole, thiazole, 1,2,3-triazole, 1,3,4-triazole, 1-oxa-2,3-diazole, 1-oxa-2,4-diazole, 1-oxa-2,5-diazole, 1-oxa-3,4-diazole, 1-thia-2,3-diazole, 1-thia-2,4-diazole, 1-thia-2,5-diazole, 1-thia-3,4-diazole, tetrazole, pyridine, pyridazine, pyrimidine, and pyrazine.

In yet a further embodiment, is a compound of Formula I, wherein R⁵ is a C₃-C₆ cycloalkyl ring; or a 3-6-membered heterocycloalkyl ring comprising 1-3 N atoms, an O atom, a S atom; or any combination thereof, and wherein R⁵ is further substituted by halogen, —CN, —NO₂, —OH, —SR⁸, —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸, —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, —OR¹⁰, substituted or unsubstituted alkyl, substituted or unsubstituted alkoxy, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl or substituted or unsubstituted heterocycloalkyl.

In one embodiment, R⁵ is a C₃-C₆cycloalkyl ring. In another embodiment, the C₃-C₆cycloalkyl ring is cyclopropyl. In another embodiment, the C₃-C₆cycloalkyl ring is cyclopentyl. In another embodiment, the C₃-C₆cycloalkyl is cyclohexyl.

In another embodiment, R⁵ is OH or CN. In a further embodiment, R⁵ is OCF₃, or CF₃.

In one embodiment, two R⁵ together with the atoms to which they are attached form a cycloalkyl group. In another embodiment, two R⁵ together with the atoms to which they are attached form a heterocycloalkyl group.

In yet another embodiment is a compound of Formula I wherein r is 0. In another embodiment, r is 1. In a further embodiment, r is 2.

In one embodiment is a compound of Formula I wherein

In another embodiment is a compound of Formula I wherein

In a further embodiment is a compound of Formula I wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula I wherein

R₆ is methyl and m is 0.

In one embodiment is a compound of Formula I wherein

is selected from:

In yet another embodiment is a compound of Formula I wherein

selected from:

In yet another embodiment is a compound of Formula I wherein

is selected from:

In one embodiment, is a compound of Formula I, wherein R⁵ is halogen, —CN, —OH, substituted or unsubstituted alkyl, —OR¹⁰, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, or substituted or unsubstituted heterocycloalkyl. In one embodiment, R⁵ is selected from F, Cl, Br, or I. In another embodiment R⁵ is F.

In another embodiment, is a compound of Formula I, wherein at least one R⁵ is —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, or substituted or unsubstituted heterocycloalkyl. In one embodiment, is a compound of Formula I, wherein at least one R⁵ is —N(R¹⁰)₂, or substituted or unsubstituted heterocycloalkyl. In yet another embodiment, is a compound of Formula I wherein at least one of R⁵ is a substituted or unsubstituted piperazine, substituted or unsubstituted piperidine, substituted or unsubstituted pyrrolidine or substituted or unsubstituted morpholine. In a further embodiment, is a compound of Formula I, wherein at least one R⁵ is —OR¹⁰. In one embodiment is a compound of Formula I, wherein at least one R⁵ is —OR¹⁰ and R¹⁰ is H. In another embodiment, R¹⁰ is alkyl selected from methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, and tert-butyl.

In one embodiment is a compound of Formula I wherein ring B is substituted with —N(R¹⁰)₂, wherein R¹⁰ is each independently selected from H and a substituted or unsubstituted heterocycloalkyl. In another embodiment is a compound of Formula I wherein ring B is substituted with —NHR¹⁰ wherein R¹⁰ is a substituted or unsubstituted piperazine, substituted or unsubstituted piperidine, substituted or unsubstituted pyrrolidine or substituted or unsubstituted morpholine. In a further embodiment is a compound of Formula I wherein ring B is substituted with —N(CH₃)R¹⁰ wherein R¹⁰ is a substituted or unsubstituted piperazine, substituted or unsubstituted piperidine, substituted or unsubstituted pyrrolidine or substituted or unsubstituted morpholine.

Also presented herein is a compound of Formula I wherein ring B is substituted with —OR¹⁰ wherein R¹⁰ is a substituted or unsubstituted heterocycloalkyl. In another embodiment is a compound of Formula I wherein ring B is substituted with —OR¹⁰ wherein R¹⁰ is a substituted or unsubstituted piperazine, substituted or unsubstituted piperidine, substituted or unsubstituted pyrrolidine or substituted or unsubstituted morpholine. In yet another embodiment is a compound of Formula I wherein ring B is substituted with at least one CF₃.

In yet another embodiment, ring B is substituted with at least two R⁵. In another embodiment, ring B is substituted with halogen and a substituted or unsubstituted heterocycloalkyl. In another embodiment, ring B is substituted with at least one F, Cl, Br, or I and a substituted or unsubstituted piperazine, substituted or unsubstituted piperidine, substituted or unsubstituted pyrrolidine, or substituted or unsubstituted morpholine.

In another aspect is a compound having the structure of Formula II or pharmaceutically acceptable salt or N-oxide thereof:

wherein:

-   -   ring T is an aryl, or a heteroaryl ring;     -   R³ is a substituted or unsubstituted cycloalkyl, a substituted         or unsubstituted heteroaryl attached to ring T via a carbon atom         of R³, or a substituted or unsubstituted heterocycloalkyl         attached to ring T via a carbon atom of R³;     -   each R⁴ is independently halogen, —CN, —NO₂, —OH, —OCF₃, —OCF₂H,         —CF₃, —SR⁸, —S(═O)R⁹, —S(═O)₂R⁹, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂,         —OR¹⁰, —C(═O)R⁸, —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted         or unsubstituted alkyl, substituted or unsubstituted         heteroalkyl, substituted or unsubstituted cycloalkyl or         substituted or unsubstituted heterocycloalkyl;     -   R⁸ is H or R⁹;     -   R⁹ is a substituted or unsubstituted alkyl, a substituted or         unsubstituted cycloalkyl, a substituted or unsubstituted         heterocycloalkyl, a substituted or unsubstituted aryl, or a         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted heterocycloalkyl, substituted or unsubstituted         aryl or substituted or unsubstituted heteroaryl, or two R¹⁰         together with the atoms to which they are attached form a         heterocycle;     -   s is 0-4;     -   ring B is aryl or heteroaryl;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, —OR¹⁰, substituted or         unsubstituted alkyl, substituted or unsubstituted heteroalkyl,         substituted or unsubstituted cycloalkyl or substituted or         unsubstituted heterocycloalkyl; and     -   r is 0-8.

In one embodiment is a compound of Formula II wherein

In another embodiment is a compound of Formula II wherein

In a further embodiment is a compound of Formula II wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula II wherein

R₆ is methyl and m is 0.

In a further embodiment is a compound having the structure of Formula III:

wherein s1 is 0 to 3 and ring T, ring B, R³, R⁴, R⁵, Q and r are described previously.

In one embodiment is a compound of Formula III wherein

In another embodiment is a compound of Formula III wherein

In a further embodiment is a compound of Formula III wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula III wherein

R₆ is methyl and m is 0.

In yet a further embodiment is a compound having the structure of Formula IV:

wherein ring B, R³, R⁴, R⁵, Q, s and r are described previously.

In one embodiment is a compound of Formula IV wherein

In another embodiment is a compound of Formula IV wherein

In a further embodiment is a compound of Formula IV wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula IV wherein

R₆ is methyl and m is 0.

In another embodiment is a compound having the structure of Formula V:

wherein ring B, R³, R⁴, R⁵, Q, s and r are described previously.

In one embodiment is a compound of Formula V wherein

In another embodiment is a compound of Formula V wherein

In a further embodiment is a compound of Formula V wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula V wherein

R₆ is methyl and m is 0.

In another embodiment is a compound having the structure of Formula Va:

wherein ring B, R³, R⁴, R⁵, Q, s and r are described previously.

In one embodiment is a compound of Formula Va wherein

In another embodiment is a compound of Formula Va wherein

In a further embodiment is a compound of Formula Va wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula Va wherein

R₆ is methyl and m is 0.

In another embodiment is a compound having the structure of Formula Vb:

wherein ring B, R³, R⁴, R⁵, Q and r are described previously.

In one embodiment is a compound of Formula Vb wherein

In another embodiment is a compound of Formula Vb wherein

In a further embodiment is a compound of Formula Vb wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula Vb wherein

R⁶ is methyl and m is 0.

In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb wherein R³ is selected from pyrrole, furan, thiophene, pyrazole, imidazole, isoxazole, oxazole, isothiazole, thiazole, 1,2,3-triazole, 1,3,4-triazole, 1-oxa-2,3-diazole, 1-oxa-2,4-diazole, 1-oxa-2,5-diazole, 1-oxa-3,4-diazole, 1-thia-2,3-diazole, 1-thia-2,4-diazole, 1-thia-2,5-diazole, 1-thia-3,4-diazole, tetrazole, pyridine, pyridazine, pyrimidine, and pyrazine.

In another embodiment, is a compound of Formula I, II, III, IV, V, Va, or Vb wherein R³ is selected from

In yet another embodiment, R³ is selected from

In yet another embodiment, R³ is selected from

In a further embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein

In another embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, where R⁵ is halogen, —CN, —OH, a substituted or unsubstituted alkyl, —OR¹⁰, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, or a substituted or unsubstituted heterocycloalkyl.

In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein at least one R⁵ is —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, or a substituted or unsubstituted heterocycloalkyl.

In another embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein at least one R⁵ is selected from:

In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein at least one R⁵ is —N(R¹⁰)₂, or a substituted or unsubstituted heterocycloalkyl. In a further embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb wherein at least one of R⁵ is a substituted or unsubstituted piperazine, a substituted or unsubstituted piperidine, a substituted or unsubstituted pyrrolidine, or a substituted or unsubstituted morpholine. In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein at least one R⁵ is —OR¹⁰. In another embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein R⁴ is independently halogen, —CN, —OH, —OCF₃, —OCF₃, —OCF₂H, —CF₃, —SR⁸, a substituted or unsubstituted alkyl, or a substituted or unsubstituted alkoxy.

In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein s is zero.

In a further embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein Q is a substituted or unsubstituted alkyl, or a substituted or unsubstituted heteroalkyl. In another embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein Q is a substituted or unsubstituted cycloalkyl, or a substituted or unsubstituted heterocycloalkyl. In a further embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein Q is a substituted or unsubstituted cycloalkylalkyl, or a substituted or unsubstituted heterocycloalkylalkyl. In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein Q is a substituted or unsubstituted aryl, or a substituted or unsubstituted heteroaryl.

In one embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein Q is a substituted or unsubstituted arylalkyl, or a substituted or unsubstituted heteroarylalkyl.

In another embodiment is a compound of Formula I, II, III, IV, V, Va, or Vb, wherein Q is selected from:

Also provided herein, in some embodiments, are compounds having the structure of Formula VI or pharmaceutically acceptable salt or N-oxide thereof:

wherein:

-   -   W is a bond;     -   R⁶ is —CN, —OH, substituted or unsubstituted alkoxy, —N(R¹⁰)₂,         substituted or unsubstituted heteroalkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   R⁷ is halogen, —CN, —OH, substituted or unsubstituted alkoxy,         —C(═O)N(R¹⁰)₂, —CO₂R¹⁶, —N(R¹⁰)₂, acyl, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl, substituted or unsubstituted heterocycloalkyl,         substituted or unsubstituted aryl or substituted or         unsubstituted heteroaryl;     -   Q is substituted or unsubstituted alkyl, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         heterocycloalkyl, substituted or unsubstituted cycloalkyl,         substituted or unsubstituted cycloalkylalkyl, substituted or         unsubstituted heterocycloalkylalkyl, substituted or         unsubstituted aryl, substituted or unsubstituted arylalkyl,         substituted or unsubstituted heteroaryl, substituted or         unsubstituted heteroarylalkyl, or substituted or unsubstituted         cycloalkyl or heterocycloalkyl fused to ring A;     -   ring A is substituted or unsubstituted aryl or heteroaryl         substituted with 0-4 R⁴; each R⁴ is independently halogen, —CN,         —NO₂, —OH, —SR⁸, —S(═O)R⁹, —S(═O)₂R⁹, —NR¹⁰S(═O)₂R⁹,         —S(═O)₂N(R¹⁶)₂, —C(═O)R⁸, —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂,         —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁶, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁶)₂,         substituted or unsubstituted alkyl, substituted or unsubstituted         alkoxy, substituted or unsubstituted heteroalkyl, substituted or         unsubstituted cycloalkyl or substituted or unsubstituted         heterocycloalkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8.

In one embodiment, is a compound having the structure of Formula VI or pharmaceutically acceptable salt or N-oxide thereof wherein:

-   -   W is a bond;     -   R⁶ is —CN, —OH, substituted or unsubstituted alkoxy, —N(R¹⁰)₂,         substituted or unsubstituted heteroalkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   R⁷ is halogen, —CN, —OH, substituted or unsubstituted alkoxy,         —C(═O)N(R¹⁰)₂, —CO₂R¹⁰, —N(R¹⁰)₂, acyl, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl, substituted or unsubstituted heterocycloalkyl,         substituted or unsubstituted aryl or substituted or         unsubstituted heteroaryl;     -   Q is substituted or unsubstituted heteroalkyl, substituted or         unsubstituted heterocycloalkyl, substituted or unsubstituted         cycloalkyl, substituted or unsubstituted cycloalkylalkyl,         substituted or unsubstituted heterocycloalkylalkyl, substituted         or unsubstituted aryl, substituted or unsubstituted arylalkyl,         substituted or unsubstituted heteroaryl, or substituted or         unsubstituted heteroarylalkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8.

In one embodiment is a compound of Formula VI wherein

In another embodiment is a compound of Formula VI wherein

In a further embodiment is a compound of Formula VI wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula VI wherein

R⁶ is methyl and m is 0.

In another embodiment is a compound of structure of Formula VI or pharmaceutically acceptable salt or N-oxide thereof wherein:

-   -   W is a bond;     -   R⁶ is —CN, —OH, substituted or unsubstituted alkoxy, —N(R¹⁰)₂,         substituted or unsubstituted heteroalkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   R⁷ is halogen, —CN, —OH, substituted or unsubstituted alkoxy,         —C(═O)N(R¹⁰)₂, —CO₂R¹⁰, —N(R¹⁰)₂, acyl, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl, substituted or unsubstituted heterocycloalkyl,         substituted or unsubstituted aryl or substituted or         unsubstituted heteroaryl;     -   Q is an unsubstituted alkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8.

In yet another embodiment is a compound of structure of Formula VI or pharmaceutically acceptable salt or N-oxide thereof wherein:

-   -   W is a bond;     -   R⁶ is —CN, —OH, substituted or unsubstituted alkoxy, —N(R¹⁰)₂,         substituted or unsubstituted heteroalkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   R⁷ is halogen, —CN, —OH, substituted or unsubstituted alkoxy,         —C(═O)N(R¹⁰)₂, —CO₂R¹⁰, —N(R¹⁰)₂, acyl, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl, substituted or unsubstituted heterocycloalkyl,         substituted or unsubstituted aryl or substituted or         unsubstituted heteroaryl;     -   Q is a substituted alkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8.

Provided herein, in some embodiments, are compounds having the structure of Formula VII or pharmaceutically acceptable salt or N-oxide thereof:

-   -   wherein:     -   W is a bond;     -   R⁶ is substituted or unsubstituted heteroalkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   R⁷ is H, halogen, —CN, —OH, substituted or unsubstituted alkyl,         substituted or unsubstituted alkoxy, —C(═O)N(R¹⁰)₂, —CO₂R¹⁰,         —N(R¹⁰)₂, acyl, substituted or unsubstituted heteroalkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted heterocycloalkyl, substituted or unsubstituted         aryl or substituted or unsubstituted heteroaryl;     -   Q is substituted or unsubstituted alkyl, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         heterocycloalkyl, substituted or unsubstituted cycloalkyl,         substituted or unsubstituted cycloalkylalkyl, substituted or         unsubstituted heterocycloalkylalkyl, substituted or         unsubstituted aryl, substituted or unsubstituted arylalkyl,         substituted or unsubstituted heteroaryl, substituted or         unsubstituted heteroarylalkyl, or substituted or unsubstituted         cycloalkyl or heterocycloalkyl fused to ring A;     -   ring A is substituted or unsubstituted aryl or heteroaryl         substituted with 0-4 R⁴;     -   each R⁴ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂—C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8.

In one embodiment is a compound of Formula VII wherein Q is substituted or unsubstituted alkyl. In a further embodiment is a compound of Formula VII wherein Q is a substituted alkyl. In yet another embodiment is a compound of Formula VII wherein Q is an unsubstituted alkyl. In a further embodiment is a compound of Formula VII wherein Q is substituted or unsubstituted heteroalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloalkylalkyl, substituted or unsubstituted heterocycloalkylalkyl, substituted or unsubstituted aryl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl.

In one embodiment is a compound of Formula VII wherein

In another embodiment is a compound of Formula VII wherein

In a further embodiment is a compound of Formula VII wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula VII wherein

R₆ is methyl and m is 0.

Provided herein, in some embodiments, are compounds having the structure of Formula VIII or pharmaceutically acceptable salt or N-oxide thereof:

-   -   wherein:     -   W is a bond;     -   R⁶ is H, or halogen;     -   R⁷ is acyl, substituted or unsubstituted heteroalkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted heterocycloalkyl or substituted or unsubstituted         heteroaryl;     -   Q is substituted or unsubstituted alkyl, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         heterocycloalkyl, substituted or unsubstituted cycloalkyl,         substituted or unsubstituted cycloalkylalkyl, substituted or         unsubstituted heterocycloalkylalkyl, substituted or         unsubstituted aryl, substituted or unsubstituted arylalkyl,         substituted or unsubstituted heteroaryl, substituted or         unsubstituted heteroarylalkyl, or substituted or unsubstituted         cycloalkyl or heterocycloalkyl fused to ring A;     -   ring A is substituted or unsubstituted aryl or heteroaryl         substituted with 0-4 R⁴;     -   each R⁴ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂—C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰—N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)R¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁹,         —OC(═O)R⁸, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8.

In one embodiment is a compound of Formula VIII wherein Q is substituted or unsubstituted alkyl. In a further embodiment is a compound of Formula VIII wherein Q is a substituted alkyl. In yet another embodiment is a compound of Formula VIII wherein Q is an unsubstituted alkyl. In a further embodiment is a compound of Formula VIII wherein Q is substituted or unsubstituted heteroalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloalkylalkyl, substituted or unsubstituted heterocycloalkylalkyl, substituted or unsubstituted aryl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl.

In one embodiment is a compound of Formula VIII wherein

In another embodiment is a compound of Formula VIII wherein

In a further embodiment is a compound of Formula VIII wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula VIII wherein

R₆ is methyl and m is 0.

Also provided herein, in some embodiments, are compounds having the structure of Formula IX or pharmaceutically acceptable salt or N-oxide thereof:

-   -   wherein:     -   W is a bond;     -   R⁶ is substituted or unsubstituted alkyl;     -   R⁷ is substituted or unsubstituted heteroalkyl, substituted or         unsubstituted cycloalkyl, or substituted or unsubstituted         heterocycloalkyl;     -   Q is substituted or unsubstituted alkyl, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         heterocycloalkyl, substituted or unsubstituted cycloalkyl,         substituted or unsubstituted cycloalkylalkyl, substituted or         unsubstituted heterocycloalkylalkyl, substituted or         unsubstituted aryl, substituted or unsubstituted arylalkyl,         substituted or unsubstituted heteroaryl, substituted or         unsubstituted heteroarylalkyl, or substituted or unsubstituted         cycloalkyl or heterocycloalkyl fused to ring A;     -   ring A is substituted or unsubstituted aryl or heteroaryl         substituted with 0-4 R⁴;

each R⁴ is independently halogen, —CN, —NO₂, —OH, —SR⁸, —S(═O)R⁹, —S(═O)₂R⁹, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸, —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted alkyl, substituted or unsubstituted alkoxy, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl or substituted or unsubstituted heterocycloalkyl;

-   -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8.

In one embodiment is a compound of Formula IX wherein Q is substituted or unsubstituted alkyl. In a further embodiment is a compound of Formula IX wherein Q is a substituted alkyl. In yet another embodiment is a compound of Formula IX wherein Q is an unsubstituted alkyl. In a further embodiment is a compound of Formula IX wherein Q is substituted or unsubstituted heteroalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloalkylalkyl, substituted or unsubstituted heterocycloalkylalkyl, substituted or unsubstituted aryl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl.

In one embodiment is a compound of Formula IX wherein

In another embodiment is a compound of Formula IX wherein

In a further embodiment is a compound of Formula IX wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula IX wherein

R⁶ is methyl and m is 0.

Provided herein, in some embodiments, are compounds having the structure of Formula X or a pharmaceutically acceptable salt or N-oxide thereof:

-   -   wherein:     -   W is a bond;     -   R⁶ is H;     -   R⁷ is acyl, substituted or unsubstituted heteroalkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted heterocycloalkyl or substituted or unsubstituted         heteroaryl;     -   Q is substituted or unsubstituted alkyl, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         heterocycloalkyl, substituted or unsubstituted cycloalkyl,         substituted or unsubstituted cycloalkylalkyl, substituted or         unsubstituted heterocycloalkylalkyl, substituted or         unsubstituted aryl, substituted or unsubstituted arylalkyl,         substituted or unsubstituted heteroaryl, substituted or         unsubstituted heteroarylalkyl, or substituted or unsubstituted         cycloalkyl or heterocycloalkyl fused to ring A; ring A is         substituted or unsubstituted aryl or heteroaryl substituted with         0-4 R⁴;     -   each R⁴ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         R¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁹,         —OC(═O)R⁸, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂), substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8.

In one embodiment is a compound of Formula X wherein Q is substituted or unsubstituted alkyl. In a further embodiment is a compound of Formula X wherein Q is a substituted alkyl. In yet another embodiment is a compound of Formula X wherein Q is an unsubstituted alkyl. In a further embodiment is a compound of Formula X wherein Q is substituted or unsubstituted heteroalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloalkylalkyl, substituted or unsubstituted heterocycloalkylalkyl, substituted or unsubstituted aryl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl.

In one embodiment is a compound of Formula X wherein

In another embodiment is a compound of Formula X wherein

In a further embodiment is a compound of Formula X wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula X wherein

is methyl and m is 0.

Provided herein, in some embodiments, are compounds having the structure of Formula XI or pharmaceutically acceptable salt or N-oxide thereof:

-   -   wherein:     -   W is N—R^(1a);     -   R^(1a) is H or substituted or unsubstituted alkyl;     -   Q is substituted or unsubstituted alkyl, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         heterocycloalkyl, substituted or unsubstituted cycloalkyl,         substituted or unsubstituted cycloalkylalkyl, substituted or         unsubstituted heterocycloalkylalkyl, substituted or         unsubstituted aryl, substituted or unsubstituted arylalkyl,         substituted or unsubstituted heteroaryl, or substituted or         unsubstituted heteroarylalkyl;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   r is 0-8;     -   R⁶ is H, halogen, —CN, —OH, substituted or unsubstituted alkyl,         substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, —N(R¹⁰)₂, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   R⁷ is H, halogen, —CN, —OH, acyl, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, —C(═O)N(R¹⁰)₂,         —CO₂R¹⁰, —N(R¹⁰)₂, substituted or unsubstituted heteroalkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted heterocycloalkyl, substituted or unsubstituted         aryl or substituted or unsubstituted heteroaryl.

In one embodiment is a compound of Formula XI wherein Q is substituted or unsubstituted alkyl. In a further embodiment is a compound of Formula XI wherein Q is a substituted alkyl. In yet another embodiment is a compound of Formula XI wherein Q is an unsubstituted alkyl. In a further embodiment is a compound of Formula XI wherein Q is substituted or unsubstituted heteroalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted cycloalkylalkyl, substituted or unsubstituted heterocycloalkylalkyl, substituted or unsubstituted aryl, substituted or unsubstituted arylalkyl, substituted or unsubstituted heteroaryl, substituted or unsubstituted heteroarylalkyl.

In one embodiment is a compound of Formula XI wherein

In another embodiment is a compound of Formula XI wherein

In a further embodiment is a compound of Formula XI wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula XI wherein

R₆ is methyl and m is 0.

In a further aspect is a compound having the structure of Formula XII or a pharmaceutically acceptable salt or N-oxide thereof:

-   -   wherein:     -   each of Y³, Y⁴ and Y⁵ are independently N—R^(1a), CR¹R², SO₂, or         C═O;     -   R^(1a) is H or substituted or unsubstituted alkyl;     -   R¹ and R² are each independently H or substituted or         unsubstituted alkyl; ring A is substituted or unsubstituted aryl         or heteroaryl;     -   each R⁴ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8;     -   s is 0-4;     -   R⁶ is H, halogen, —CN, —OH, substituted or unsubstituted alkyl,         substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, —N(R¹⁰)₂, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   R⁷ is H, halogen, —CN, —OH, acyl, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, —C(═O)N(R¹⁰)₂,         —CO₂R¹⁰, —N(R¹⁰)₂, substituted or unsubstituted heteroalkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted heterocycloalkyl, substituted or unsubstituted         aryl or substituted or unsubstituted heteroaryl.

In one embodiment is a compound of Formula XII wherein

In another embodiment is a compound of Formula XII wherein

In a further embodiment is a compound of Formula XII wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula XII wherein

R₆ is methyl and m is 0.

In some embodiments is a compound of Formula XIII or a pharmaceutically acceptable salt or N-oxide thereof:

wherein:

-   -   R^(1a) is H or substituted or unsubstituted alkyl;     -   ring A is substituted or unsubstituted aryl or heteroaryl;     -   each R⁴ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂,         —C(═O)N(R¹⁰)₂—NR¹⁰C(═O)R¹⁰—NR¹⁰C(═O)OR¹⁹, —NR¹⁰C(═O)N(R¹⁰)₂,         substituted or unsubstituted alkyl, substituted or unsubstituted         alkoxy, substituted or unsubstituted heteroalkyl, substituted or         unsubstituted cycloalkyl or substituted or unsubstituted         heterocycloalkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         allyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8;     -   s is 0-4;     -   R⁶ is H, halogen, —CN, —OH, substituted or unsubstituted alkyl,         substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, —N(R¹⁰)₂, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   R⁷ is H, halogen, —CN, —OH, acyl, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, —C(═O)N(R¹⁰)₂,         —CO₂R¹⁰, —N(R¹⁰)₂, substituted or unsubstituted heteroalkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted heterocycloalkyl, substituted or unsubstituted         aryl or substituted or unsubstituted heteroaryl.

In one embodiment is a compound of Formula XIII wherein

In another embodiment is a compound of Formula XIII wherein

In a further embodiment is a compound of Formula XIII wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula XIII wherein

R₆ is methyl and m is 0.

In some embodiments is a compound of Formula XIV or a pharmaceutically acceptable salt or N-oxide thereof:

wherein:

-   -   p is 1, 2 or 3; R¹ and R² are each independently H or         substituted or unsubstituted alkyl; or     -   R¹ and R² together with the carbon to which they are attached         form a C₃-C₆ cycloalkyl ring;     -   R^(1a) is H or substituted or unsubstituted alkyl;     -   ring A is substituted or unsubstituted aryl or heteroaryl;     -   each R⁴ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —NR¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8;     -   s is 0-4;     -   R⁶ is H, halogen, —CN, —OH, substituted or unsubstituted alkyl,         substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, —N(R¹⁰)₂, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   R⁷ is H, halogen, —CN, —OH, acyl, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, —C(═O)N(R¹⁰)₂,         —CO₂R¹⁰, —N(R¹⁰)₂, substituted or unsubstituted heteroalkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted heterocycloalkyl, substituted or unsubstituted         aryl or substituted or unsubstituted heteroaryl.

In some embodiments of Formula XIV, ring A is a heteroaryl ring. In some embodiments of Formula XIV, ring A is an aryl ring. In some embodiments of Formula XIV, ring A is a heterocycloalkyl ring. In some embodiments of Formula XIV, ring A is a cycloalkyl ring.

In one embodiment is a compound of Formula XIV wherein

In another embodiment is a compound of Formula XIV wherein

In a further embodiment is a compound of Formula XIV wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula XIV wherein

R₆ is methyl and m is 0.

In some embodiments is a compound of Formula XV or a pharmaceutically acceptable salt or N-oxide thereof:

wherein:

-   -   each of Y³, Y⁴ and Y⁵ are independently N—R^(1a), CR¹R², SO₂, or         C═O;     -   R^(1a) is H or substituted or unsubstituted alkyl;     -   R¹ and R² are each independently H or substituted or         unsubstituted alkyl;     -   each R⁴ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C(═)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   R⁸ is H or substituted or unsubstituted alkyl;     -   R⁹ is substituted or unsubstituted alkyl, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   each R¹⁰ is independently H, substituted or unsubstituted alkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted aryl or substituted or unsubstituted heteroaryl,         or two R¹⁰ together with the atoms to which they are attached         form a heterocycle;     -   ring B is aryl or heteroaryl substituted with R⁵;     -   each R⁵ is independently halogen, —CN, —NO₂, —OH, —SR⁸,         —S(═O)R⁹, —S(═O)₂R⁹, NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —C(═O)R⁸,         —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰,         —NR¹⁰C)═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, substituted or unsubstituted         cycloalkyl or substituted or unsubstituted heterocycloalkyl;     -   r is 0-8;     -   s is 0-4;     -   R⁶ is H, halogen, —CN, —OH, substituted or unsubstituted alkyl,         substituted or unsubstituted alkoxy, substituted or         unsubstituted heteroalkyl, —N(R¹⁰)₂, substituted or         unsubstituted cycloalkyl, substituted or unsubstituted aryl or         substituted or unsubstituted heteroaryl;     -   R⁷ is H, halogen, —CN, —OH, acyl, substituted or unsubstituted         alkyl, substituted or unsubstituted alkoxy, —C(═O)N(R¹⁰)₂,         —CO₂R¹⁰, —N(R¹⁰)₂, substituted or unsubstituted heteroalkyl,         substituted or unsubstituted cycloalkyl, substituted or         unsubstituted heterocycloalkyl, substituted or unsubstituted         aryl or substituted or unsubstituted heteroaryl.

In one embodiment is a compound of Formula XV wherein

In another embodiment is a compound of Formula XV wherein

In a further embodiment is a compound of Formula XV wherein

and R₆ is C₁-C₆alkyl, and m is 0, 1, or 2. In a further embodiment is a compound of Formula XV wherein

R₆ is methyl and m is 0.

In some embodiments, the compound has the structure of Formula XVA, Formula XVB, Formula XVC or Formula XVD or a pharmaceutically acceptable salt or N-oxide thereof:

-   -   wherein:     -   each R¹¹ is independently H, halogen, substituted or         unsubstituted alkyl, substituted or unsubstituted alkoxy, or two         R¹¹ together with the carbon atom to which they are attached         form C═O; and     -   k is 1-4.

In a further aspect is a compound having the structure:

or a pharmaceutically acceptable salt, solvate, or N-oxide thereof.

In one aspect is a compound having the structure:

wherein: R₁ is a 5- or 6-membered heteroaryl group attached to the phenyl group via a carbon atom of R₁ and optionally substituted with at least one R₄; R₄ and R₅ are each independently selected from halogen, —CN, —NO₂, —OH, —OCF₃, —OCF₂H, —CF₃, —SR⁸, —S(═O)R⁹, —S(═O)₂R⁹, —NR¹⁰S(═O)₂R⁹, —S(═O)₂N(R¹⁰)₂, —OR¹⁰, —C(═O)R⁸, —OC(═O)R⁹, —CO₂R¹⁰, —N(R¹⁰)₂, —C(═O)N(R¹⁰)₂, —NR¹⁰C(═O)R¹⁰, —NR¹⁰C(═O)OR¹⁰, —NR¹⁰C(═O)N(R¹⁰)₂, substituted or unsubstituted alkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted cycloalkyl, and substituted or unsubstituted heterocycloalkyl;

R₇ is

R₆ is H or substituted or unsubstituted alkyl; n and m are each independently an integer from 0 to 4; R₇ is substituted or unsubstituted alkyl-N(R₈)₂;

R₈ is H or R₉;

R₉ is a substituted or unsubstituted alkyl, a substituted or unsubstituted cycloalkyl, a substituted or unsubstituted heterocycloalkyl, a substituted or unsubstituted aryl, or a substituted or unsubstituted heteroaryl; each R₁₀ is independently H, substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl or substituted or unsubstituted heteroaryl, or two R₁₀ together with the atoms to which they are attached form a heterocycle; R₃ is a substituted or unsubstituted alkyl; or a pharmaceutically acceptable salt, solvate or N-oxide thereof.

In another embodiment R₁ is a 5-membered heteroaryl group attached to the phenyl group via a carbon atom of R₁. In another embodiment R₁ is 6-membered heteroaryl group attached to the phenyl group via a carbon atom of R₁. In a further embodiment, the 5-membered or 6-membered heteroaryl group is substituted with at least one R₄ selected from halogen, —CN, —NO₂, —OH, —OCF₃, —OCF₂H, —CF₃, —SR₈, —S(═O)R₉, —S(═O)₂R₉, —NR₁₀S(═O)₂R₉, —S(═O)₂N(R₁₀)₂, —OR¹⁰, —C(═O)R₈, —OC(═O)R₉, —CO₂R₁₀, —N(R₁₀)₂, —C(═O)N(R₁₀)₂, —NR₁₀C(═O)R¹⁰, —N R₁₀C(═O)OR₁₀, —NR₁₀C(═O)N(R¹⁰)₂, substituted or unsubstituted alkyl. In another embodiment the 5- or 6-membered heteroaryl group is substituted with at least one C₁-C₆alkyl group. In another embodiment, the C₁-C₆alkyl group is methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, or tert-butyl.

In another embodiment R₂ is

wherein R₆ is H, or C₁-C₆alkyl selected from methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-propyl, and tert-butyl. In another embodiment R₂ is

wherein R₆ is C₁-C₆alkyl selected from methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-propyl, and tert-butyl. In a further embodiment, R₆ is methyl. In a further embodiment, R₆ is ethyl. In yet a further embodiment, R₆ is iso-propyl. In yet another embodiment, R₆ is hydrogen. In a further embodiment, R₅ is a halogen. In another embodiment, R₅ is Cl. In a further embodiment, R₅ is F. In yet a further embodiment, R₅ is Br. In another embodiment, m is 1 and n is 0. In another embodiment, m is 0 and n is 0.

In one embodiment R₃ is methyl. In another embodiment, R₃ is ethyl.

In one aspect is a compound having the structure:

wherein: R₁ is selected from:

R₂ is selected from:

and R₃ is methyl or ethyl; or a pharmaceutically acceptable salt, solvate or N-oxide thereof.

In some embodiments, a PAK inhibitor is a small molecule. As referred to herein, a “small molecule” is an organic molecule that is less than about 5 kilodaltons (kDa) in size. In some embodiments, the small molecule is less than about 4 kDa, 3 kDa, about 2 kDa, or about 1 kDa. In some embodiments, the small molecule is less than about 800 daltons (Da), about 600 Da, about 500 Da, about 400 Da, about 300 Da, about 200 Da, or about 100 Da. In some embodiments, a small molecule is less than about 4000 g/mol, less than about 3000 g/mol, 2000 g/mol, less than about 1500 g/mol, less than about 1000 g/mol, less than about 800 g/mol, or less than about 500 g/mol. In some embodiments, small molecules are non-polymeric. Typically, small molecules are not proteins, polypeptides, polynucleotides, oligonucleotides, polysaccharides, glycoproteins, or proteoglycans, but includes peptides of up to about 40 amino acids. A derivative of a small molecule refers to a molecule that shares the same structural core as the original small molecule, but which is prepared by a series of chemical reactions from the original small molecule. As one example, a pro-drug of a small molecule is a derivative of that small molecule. An analog of a small molecule refers to a molecule that shares the same or similar structural core as the original small molecule, and which is synthesized by a similar or related route, or art-recognized variation, as the original small molecule.

In certain embodiments, compounds described herein have one or more chiral centers. As such, all stereoisomers are envisioned herein. In various embodiments, compounds described herein are present in optically active or racemic forms. It is to be understood that the compounds described herein encompass racemic, optically-active, regioisomeric and stereoisomeric forms, or combinations thereof that possess the therapeutically useful properties described herein. Preparation of optically active forms is achieve in any suitable manner, including by way of non-limiting example, by resolution of the racemic form by recrystallization techniques, by synthesis from optically-active starting materials, by chiral synthesis, or by chromatographic separation using a chiral stationary phase. In some embodiments, mixtures of one or more isomer is utilized as the therapeutic compound described herein. In certain embodiments, compounds described herein contains one or more chiral centers. These compounds are prepared by any means, including enantioselective synthesis and/or separation of a mixture of enantiomers and/or diastereomers. Resolution of compounds and isomers thereof is achieved by any means including, by way of non-limiting example, chemical processes, enzymatic processes, fractional crystallization, distillation, chromatography, and the like.

In various embodiments, pharmaceutically acceptable salts described herein include, by way of non-limiting example, a nitrate, chloride, bromide, phosphate, sulfate, acetate, hexafluorophosphate, citrate, gluconate, benzoate, propionate, butyrate, sulfosalicylate, maleate, laurate, malate, fumarate, succinate, tartrate, amsonate, pamoate, p-toluenenesulfonate, mesylate and the like. Furthermore, pharmaceutically acceptable salts include, by way of non-limiting example, alkaline earth metal salts (e.g., calcium or magnesium), alkali metal salts (e.g., sodium-dependent or potassium), ammonium salts and the like.

Compounds described herein also include isotopically-labeled compounds wherein one or more atoms is replaced by an atom having the same atomic number, but an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes suitable for inclusion in the compounds described herein include and are not limited to ²H, ³H, ¹¹C, ¹³C, ¹⁴C, ³⁶Cl, ¹⁸F, ¹²³I, ¹²⁵I ¹³N, ¹⁵N, ¹⁵O, ¹⁷O, ¹⁸O, ³²P, ³⁵S or the like. In some embodiments, isotopically-labeled compounds are useful in drug and/or substrate tissue distribution studies. In some embodiments, substitution with heavier isotopes such as deuterium affords certain therapeutic advantages resulting from greater metabolic stability (for example, increased in vivo half-life or reduced dosage requirements). In some embodiments, substitution with positron emitting isotopes, such as ¹¹C, ¹⁸F, ¹⁵O and ¹³N, is useful in Positron Emission Topography (PET) studies for examining substrate receptor occupancy. Isotopically-labeled compounds are prepared by any suitable method or by processes using an appropriate isotopically-labeled reagent in place of the non-labeled reagent otherwise employed.

The compounds described herein, and other related compounds having different substituents are synthesized using techniques and materials described herein and as described, for example, in Fieser and Fieser's Reagents for Organic Synthesis, Volumes 1-17 (John Wiley and Sons, 1991); Rodd's Chemistry of Carbon Compounds, Volumes 1-5 and Supplementals (Elsevier Science Publishers, 1989); Organic Reactions, Volumes 1-40 (John Wiley and Sons, 1991), Larock's Comprehensive Organic Transformations (VCH Publishers Inc., 1989), March, ADVANCED ORGANIC CHEMISTRY 4^(th) Ed., (Wiley 1992); Carey and Sundberg, ADVANCED ORGANIC CHEMISTRY 4^(th) Ed., Vols. A and B (Plenum 2000, 2001), and Green and Wuts, PROTECTIVE GROUPS IN ORGANIC SYNTHESIS 3^(rd) Ed., (Wiley 1999) (all of which are incorporated by reference for such disclosure). General methods for the preparation of compound as described herein are modified by the use of appropriate reagents and conditions, for the introduction of the various moieties found in the formula as provided herein. As a guide the following synthetic methods are utilized.

Compounds described herein are synthesized using any suitable procedures starting from compounds that are available from commercial sources, or are prepared using procedures described herein.

Formation of Covalent Linkages by Reaction of an Electrophile with a Nucleophile

The compounds described herein are modified using various electrophiles and/or nucleophiles to form new functional groups or substituents. Table A entitled “Examples of Covalent Linkages and Precursors Thereof” lists selected non-limiting examples of covalent linkages and precursor functional groups which yield the covalent linkages. Table A is used as guidance toward the variety of electrophiles and nucleophiles combinations available that provide covalent linkages. Precursor functional groups are shown as electrophilic groups and nucleophilic groups.

TABLE A Examples of Covalent Linkages and Precursors Thereof Covalent Linkage Product Electrophile Nucleophile Carboxamides Activated esters amines/anilines Carboxamides acyl azides amines/anilines Carboxamides acyl halides amines/anilines Esters acyl halides alcohols/phenols Esters acyl nitriles alcohols/phenols Carboxamides acyl nitriles amines/anilines Imines Aldehydes amines/anilines Hydrazones aldehydes or ketones Hydrazines Oximes aldehydes or ketones Hydroxylamines Alkyl amines alkyl halides amines/anilines Esters alkyl halides carboxylic acids Thioethers alkyl halides Thiols Ethers alkyl halides alcohols/phenols Thioethers alkyl sulfonates Thiols Esters alkyl sulfonates carboxylic acids Ethers alkyl sulfonates alcohols/phenols Esters Anhydrides alcohols/phenols Carboxamides Anhydrides amines/anilines Thiophenols aryl halides Thiols Aryl amines aryl halides Amines Thioethers Azindines Thiols Boronate esters Boronates Glycols Carboxamides carboxylic acids amines/anilines Esters carboxylic acids Alcohols hydrazines Hydrazides carboxylic acids N-acylureas or Anhydrides carbodiimides carboxylic acids Esters diazoalkanes carboxylic acids Thioethers Epoxides Thiols Thioethers haloacetamides Thiols Ammotriazines halotriazines amines/anilines Triazinyl ethers halotriazines alcohols/phenols Amidines imido esters amines/anilines Ureas Isocyanates amines/anilines Urethanes Isocyanates alcohols/phenols Thioureas isothiocyanates amines/anilines Thioethers Maleimides Thiols Phosphite esters phosphoramidites Alcohols Silyl ethers silyl halides Alcohols Alkyl amines sulfonate esters amines/anilines Thioethers sulfonate esters Thiols Esters sulfonate esters carboxylic acids Ethers sulfonate esters Alcohols Sulfonamides sulfonyl halides amines/anilines Sulfonate esters sulfonyl halides phenols/alcohols

Use of Protecting Groups

In the reactions described, it is necessary to protect reactive functional groups, for example hydroxy, amino, imino, thio or carboxy groups, where these are desired in the final product, in order to avoid their unwanted participation in reactions. Protecting groups are used to block some or all of the reactive moieties and prevent such groups from participating in chemical reactions until the protective group is removed. In some embodiments it is contemplated that each protective group be removable by a different means. Protective groups that are cleaved under totally disparate reaction conditions fulfill the requirement of differential removal.

In some embodiments, protective groups are removed by acid, base, reducing conditions (such as, for example, hydrogenolysis), and/or oxidative conditions. Groups such as trityl, dimethoxytrityl, acetal and t-butyldimethylsilyl are acid labile and are used to protect carboxy and hydroxy reactive moieties in the presence of amino groups protected with Cbz groups, which are removable by hydrogenolysis, and Fmoc groups, which are base labile. Carboxylic acid and hydroxy reactive moieties are blocked with base labile groups such as; but not limited to, methyl, ethyl, and acetyl in the presence of amines blocked with acid labile groups such as t-butyl carbamate or with carbamates that are both acid and base stable but hydrolytically removable.

In some embodiments carboxylic acid and hydroxy reactive moieties are blocked with hydrolytically removable protective groups such as the benzyl group, while amine groups capable of hydrogen bonding with acids are blocked with base labile groups such as Fmoc. Carboxylic acid reactive moieties are protected by conversion to simple ester compounds as exemplified herein, which include conversion to alkyl esters, or are blocked with oxidatively-removable protective groups such as 2,4-dimethoxybenzyl, while co-existing amino groups are blocked with fluoride labile silyl carbamates.

Allyl blocking groups are useful in the presence of acid- and base-protecting groups since the former are stable and are subsequently removed by metal or Pd⁰-acid catalysts. For example, an allyl-blocked carboxylic acid is deprotected with a Pd⁰-catalyzed reaction in the presence of acid labile t-butyl carbamate or base-labile acetate amine protecting groups. Yet another form of protecting group is a resin to which a compound or intermediate is attached. As long as the residue is attached to the resin, that functional group is blocked and does not react. Once released from the resin, the functional group is available to react.

Typically blocking/protecting groups are selected from:

Other protecting groups, plus a detailed description of techniques applicable to the creation of protecting groups and their removal are described in Greene and Wuts, Protective Groups in Organic Synthesis, 3rd Ed., John Wiley & Sons, New York, N.Y., 1999, and Kocienski, Protective Groups, Thieme Verlag, New York, N.Y., 1994, which are incorporated herein by reference for such disclosure.

CERTAIN DEFINITIONS

As used herein the term “Treatment”, “treat”, or “treating” includes achieving a therapeutic benefit and/or a prophylactic benefit. Therapeutic benefit is meant to include eradication or amelioration of the underlying disorder or condition being treated. For example, in an individual with Huntington's disease, therapeutic benefit includes alleviation or partial and/or complete halting of the progression of the disease, or partial or complete reversal of the disease. Also, a therapeutic benefit is achieved with the eradication or amelioration of one or more of the physiological or psychological symptoms associated with the underlying condition such that an improvement is observed in the patient, notwithstanding the fact that the patient is still affected by the condition. For example, in an individual suffering from epilepsy, therapeutic benefit includes alleviation or partial and/or complete halting of seizures, or reduction in frequency of seizures. A prophylactic benefit of treatment includes prevention of a condition, retarding the progress of a condition, or decreasing the likelihood of occurrence of a condition. As used herein, “treat”, “treating” or “treatment” includes prophylaxis.

As used herein, the phrase “abnormal spine size” refers to dendritic spine volumes or dendritic spine surface areas (e.g., volumes or surface areas of the spine heads and/or spine necks) associated with CNS disorders that deviate significantly relative to spine volumes or surface areas in the same brain region (e.g., the CAI region, the prefrontal cortex) in a normal individual (e.g., a mouse, rat, or human) of the same age; such abnormalities are determined as appropriate, by methods including, e.g., tissue samples, relevant animal models, post-mortem analyses, or other model systems.

The phrase “defective spine morphology” or “abnormal spine morphology” or “aberrant spine morphology” refers to abnormal dendritic spine shapes, volumes, surface areas, length, width (e.g., diameter of the neck), spine head diameter, spine head volume, spine head surface area, spine density, ratio of mature to immature spines, ratio of spine volume to spine length, or the like that is associated with a CNS disorder relative to the dendritic spine shapes, volumes, surface areas, length, width (e.g., diameter of the neck), spine density, ratio of mature to immature spines, ratio of spine volume to spine length, or the like observed in the same brain region in a normal individual (e.g., a mouse, rat, or human) of the same age; such abnormalities or defects are determined as appropriate, by methods including, e.g., tissue samples, relevant animal models, post-mortem analyses, or other model systems.

The phrase “abnormal spine function” or “defective spine function” or “aberrant spine function” refers to a defect of dendritic spines to undergo stimulus-dependent morphological or functional changes (e.g., following activation of AMPA and/or NMDA receptors, LTP, LTD, etc) associated with CNS disorders as compared to dendritic spines in the same brain region in a normal individual of the same age. The “defect” in spine function includes, e.g., a reduction in dendritic spine plasticity, (e.g., an abnormally small change in dendritic spine morphology or actin re-arrangement in the dendritic spine), or an excess level of dendritic plasticity, (e.g., an abnormally large change in dendritic spine morphology or actin re-arrangement in the dendritic spine). Such abnormalities or defects are determined as appropriate, by methods including, e.g., tissue samples, relevant animal models, post-mortem analyses, or other model systems.

The phrase “abnormal spine motility” refers to a significant low or high movement of dendritic spines associated with a CNS disorder as compared to dendritic spines in the same brain region in a normal individual of the same age. Any defect in spine morphology (e.g., spine length, density or the like) or synaptic plasticity or synaptic function (e.g., LTP, LTD or the like) or spine motility occurs in any region of the brain, including, for example, the frontal cortex, the hippocampus, the amygdala, the CAI region, the prefrontal cortex or the like. Such abnormalities or defects are determined as appropriate, by methods including, e.g., tissue samples, relevant animal models, post-mortem analyses, or other model systems.

As used herein, the phrase “biologically active” refers to a characteristic of any substance that has activity in a biological system and/or organism. For instance, a substance that, when administered to an organism, has a biological effect on that organism is considered to be biologically active. In particular embodiments, where a protein or polypeptide is biologically active, a portion of that protein or polypeptide that shares at least one biological activity of the protein or polypeptide is typically referred to as a “biologically active” portion.

As described herein, a CNS disorder is a disorder that can affect either the spinal cord or brain. By way of example only, CNS disorder include Schizophrenia, Psychotic disorder, schizoaffective disorder, schizophreniform, Alzheimer's disease, Age-related cognitive decline, Mild cognitive impairment, cognitive decline associated with menopause, Parkinson's Disease, Huntington's Disease, Substance abuse and substance dependence, Fragile X, Rett's syndrome, Angelman Syndrome, Asperger's Syndrome, Autism, Autism Spectrum Disorders, Neurofibromatosis I, Neurofibromatosis II, Tuberous sclerosis, Clinical Depression, Bipolar Disorder, Mania, Epilepsy, Mental retardation, Down's syndrome, Niemann-Pick disease, Spongiform encephalitis, Lafora disease, Maple syrup urine disease, maternal phenylketonuria, atypical phenylketonuria, Generalized Anxiety Disorder, Turner Syndrome, Lowe Syndrome, Obsessive-compulsive disorder, Panic disorder, Phobias, Posttraumatic Stress Disorder, Anorexia Nervosa, and Bulimia Nervosa.

As used herein, Mental retardation is a disorder characterized by significantly impaired cognitive function and deficits in adaptive behaviors. By way of example only, mental retardation is Down's syndrome, Fetal alcohol syndrome, Klinefelter's syndrome, congenital hypothyroidism, Williams syndrome, Smith-Lemli-Opitz syndrome, Prader-Willi syndrome Phelan-McDermid syndrome, Mowat-Wilson syndrome, ciliopathy or Lowe syndrome.

As used herein, the term “subcortical dementia” refers to symptoms related to Huntington's disease (e.g., deficits in executive functions such as planning, cognitive flexibility, abstract thinking, rule acquisition, initiating appropriate actions, inhibiting inappropriate actions; memory deficits such as short-term memory deficits, long-term memory difficulties, deficits in episodic (memory of one's life), procedural (memory of the body of how to perform an activity) and working memory, and the like). In some instances, “progression toward dementia” is identified, monitored or diagnosed by neuropsychological or behavioral testing. In other instances, “progression toward dementia” is identified, monitored or diagnosed by neuroimaging or brain scans.

As used herein, the term “effective amount” is an amount, which when administered systemically, is sufficient to effect beneficial or desired results, such as beneficial or desired clinical results, or enhanced cognition, memory, mood, or other desired effects. An effective amount is also an amount that produces a prophylactic effect, e.g., an amount that delays, reduces, or eliminates the appearance of a pathological or undesired condition associated with a CNS disorder. An effective amount is optionally administered in one or more administrations. In terms of treatment, an “effective amount” of a composition described herein is an amount that is sufficient to palliate, alleviate, ameliorate, stabilize, reverse or slow the progression of a CNS disorder e.g., cognitive decline toward dementia, mental retardation or the like. An “effective amount” includes any PAK inhibitor used alone or in conjunction with one or more agents used to treat a disease or disorder. An “effective amount” of a therapeutic agent as described herein will be determined by a patient's attending physician or other medical care provider. Factors which influence what a therapeutically effective amount will be include, the absorption profile (e.g., its rate of uptake into the brain) of the PAK inhibitor, time elapsed since the initiation of disease, and the age, physical condition, existence of other disease states, and nutritional status of an individual being treated. Additionally, other medication the patient is receiving, e.g., antidepressant drugs used in combination with a PAK inhibitor, will typically affect the determination of the therapeutically effective amount of the therapeutic agent to be administered.

As used herein, the term “inhibitor” refers to a molecule which is capable of inhibiting (including partially inhibiting or allosteric inhibition) one or more of the biological activities of a target molecule, e.g., a p21-activated kinase. Inhibitors, for example, act by reducing or suppressing the activity of a target molecule and/or reducing or suppressing signal transduction. In some embodiments, a PAK inhibitor described herein causes substantially complete inhibition of one or more PAKs. In some embodiments, the phrase “partial inhibitor” refers to a molecule which can induce a partial response for example, by partially reducing or suppressing the activity of a target molecule and/or partially reducing or suppressing signal transduction. In some instances, a partial inhibitor mimics the spatial arrangement, electronic properties, or some other physicochemical and/or biological property of the inhibitor. In some instances, in the presence of elevated levels of an inhibitor, a partial inhibitor competes with the inhibitor for occupancy of the target molecule and provides a reduction in efficacy, relative to the inhibitor alone. In some embodiments, a PAK inhibitor described herein is a partial inhibitor of one or more PAKs. In some embodiments, a PAK inhibitor described herein is an allosteric modulator of PAK. In some embodiments, a PAK inhibitor described herein blocks the p21 binding domain of PAK. In some embodiments, a PAK inhibitor described herein blocks the ATP binding site of PAK. In some embodiments, a PAK inhibitor is a “Type II” kinase inhibitor. In some embodiment a PAK inhibitor stabilizes PAK in its inactive conformation. In some embodiments, a PAK inhibitor stabilizes the “DFG-out” conformation of PAK.

In some embodiments, PAK inhibitors reduce, abolish, and/or remove the binding between PAK and at least one of its natural binding partners (e.g., Cdc42 or Rac). In some instances, binding between PAK and at least one of its natural binding partners is stronger in the absence of a PAK inhibitor (by e.g., 90%, 80%, 70%, 60%, 50%, 40%, 30% or 20%) than in the presence of a PAK inhibitor. Alternatively or additionally, PAK inhibitors inhibit the phosphotransferase activity of PAK, e.g., by binding directly to the catalytic site or by altering the conformation of PAK such that the catalytic site becomes inaccessible to substrates. In some embodiments, PAK inhibitors inhibit the ability of PAK to phosphorylate at least one of its target substrates, e.g., LIM kinase 1 (LIMK1), myosin light chain kinase (MLCK), cortactin; or itself PAK inhibitors include inorganic and/or organic compounds.

In some embodiments, PAK inhibitors described herein increase dendritic spine length. In some embodiments, PAK inhibitors described herein decrease dendritic spine length. In some embodiments, PAK inhibitors described herein increase dendritic neck diameter. In some embodiments, PAK inhibitors described herein decrease dendritic neck diameter. In some embodiments, PAK inhibitors described herein increase dendritic spine head diameter. In some embodiments, PAK inhibitors described herein decrease dendritic spine head diameter. In some embodiments, PAK inhibitors described herein increase dendritic spine head volume. In some embodiments, PAK inhibitors described herein decrease dendritic spine head volume. In some embodiments, PAK inhibitors described herein increase dendritic spine surface area. In some embodiments, PAK inhibitors described herein decrease dendritic spine surface area. In some embodiments, PAK inhibitors described herein increase dendritic spine density. In some embodiments, PAK inhibitors described herein decrease dendritic spine density. In some embodiments, PAK inhibitors described herein increase the number of mushroom shaped spines. In some embodiments, PAK inhibitors described herein decrease the number of mushroom shaped spines.

In some embodiments, a PAK inhibitor suitable for the methods described herein is a direct PAK inhibitor. In some embodiments, a PAK inhibitor suitable for the methods described herein is an indirect PAK inhibitor. In some embodiments, a PAK inhibitor suitable for the methods described herein decreases PAK activity relative to a basal level of PAK activity by about 1.1 fold to about 100 fold, e.g., to about 1.2 fold, 1.5 fold, 1.6 fold, 1.7 fold, 2.0 fold, 3.0 fold, 5.0 fold, 6.0 fold, 7.0 fold, 8.5 fold, 9.7 fold, 10 fold, 12 fold, 14 fold, 15 fold, 20 fold, 30 fold, 40 fold, 50 fold, 60 fold, 70 fold, 90 fold, 95 fold, or by any other amount from about 1.1 fold to about 100 fold relative to basal PAK activity. In some embodiments, the PAK inhibitor is a reversible PAK inhibitor. In other embodiments, the PAK inhibitor is an irreversible PAK inhibitor. Direct PAK inhibitors are optionally used for the manufacture of a medicament for treating a CNS disorder.

In some embodiments, a PAK inhibitor used for the methods described herein has in vitro ED₅₀ for PAK activation of less than 100 μM (e.g., less than 10 μM, less than 5 μM, less than 4 μM, less than 3 μM, less than 1 μM, less than 0.8 μM, less than 0.6 μM, less than 0.5 μM, less than 0.4 μM, less than 0.3 μM, less than less than 0.2 μM, less than 0.1 μM, less than 0.08 μM, less than 0.06 μM, less than 0.05 μM, less than 0.04 μM, less than 0.03 μM, less than less than 0.02 μM, less than 0.01 μM, less than 0.0099 μM, less than 0.0098 μM, less than 0.0097 μM, less than 0.0096 μM, less than 0.0095 μM, less than 0.0094 μM, less than 0.0093 μM, less than 0.00092 μM, or less than 0.0090 μM).

In some embodiments, a PAK inhibitor used for the methods described herein has in vitro ED₅₀ for PAK activation of less than 100 μM (e.g., less than 10 μM, less than 5 μM, less than 4 μM, less than 3 μM, less than 1 μM, less than 0.8 μM, less than 0.6 μM, less than 0.5 μM, less than 0.4 μM, less than 0.3 μM, less than less than 0.2 μM, less than 0.1 μM, less than 0.08 μM, less than 0.06 μM, less than 0.05 μM, less than 0.04 μM, less than 0.03 μM, less than less than 0.02 μM, less than 0.01 μM, less than 0.0099 μM, less than 0.0098 μM, less than 0.0097 μM, less than 0.0096 μM, less than 0.0095 μM, less than 0.0094 μM, less than 0.0093 μM, less than 0.00092 μM, or less than 0.0090 μM).

As used herein, synaptic function refers to synaptic transmission and/or synaptic plasticity, including stabilization of synaptic plasticity. As used herein, “defect in synaptic plasticity” or “aberrant synaptic plasticity” refers to abnormal synaptic plasticity following stimulation of that synapse. In some embodiments, a defect in synaptic plasticity is a decrease in LTP. In some embodiments, a defect in synaptic plasticity is an increase in LTD. In some embodiments, a defect in synaptic plasticity is erratic (e.g., fluctuating, randomly increasing or decreasing) synaptic plasticity. In some instances, measures of synaptic plasticity are LTP and/or LTD (induced, for example, by theta-burst stimulation, high-frequency stimulation for LTP, low-frequency (e.g., e.g., 1 Hz) stimulation for LTD) and LTP and/or LTD after stabilization. In some embodiments, stabilization of LTP and/or LTD occurs in any region of the brain including the frontal cortex, the hippocampus, the prefrontal cortex, the amygdala or any combination thereof.

As used herein “stabilization of synaptic plasticity” refers to stable LTP or LTD following induction (e.g., by theta-burst stimulation, high-frequency stimulation for LTP, low-frequency (e.g., e.g., 1 Hz) stimulation for LTD).

“Aberrant stabilization of synaptic transmission” (for example, aberrant stabilization of LTP or LTD), refers to failure to establish a stable baseline of synaptic transmission following an induction paradigm (e.g., by theta-burst stimulation, high-frequency stimulation for LTP, low-frequency (e.g., 1 Hz) stimulation for LTD) or an extended period of vulnerability to disruption by pharmacological or electrophysiological means

As used herein “synaptic transmission” or “baseline synaptic transmission” refers to the EPSP and/or IPSP amplitude and frequency, neuronal excitability or population spike thresholds of a normal individual (e.g., an individual not suffering from a CNS disorder) or that predicted for an animal model for a normal individual. As used herein “aberrant synaptic transmission” or “defective synaptic transmission” refers to any deviation in synaptic transmission compared to synaptic transmission of a normal individual or that predicted for an animal model for a normal individual. In some embodiments, an individual suffering from a CNS disorder has a defect in baseline synaptic transmission that is a decrease in baseline synaptic transmission compared to the baseline synaptic transmission in a normal individual or that predicted for an animal model for a normal individual. In some embodiments, an individual suffering from a CNS disorder has a defect in baseline synaptic transmission that is an increase in baseline synaptic transmission compared to the baseline synaptic transmission in a normal individual or that predicted for an animal model for a normal individual.

As used herein “sensorimotor gating” is assessed, for example, by measuring prepulse inhibition (PPI) and/or habituation of the human startle response. In some embodiments, a defect in sensorimotor gating is a deficit in sensorimotor gating. In some embodiments, a defect in sensorimotor gating is an enhancement of sensorimotor gating.

As used herein, “normalization of aberrant synaptic plasticity” refers to a change in aberrant synaptic plasticity in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder to a level of synaptic plasticity that is substantially the same as the synaptic plasticity of a normal individual or to that predicted from an animal model for a normal individual. As used herein, substantially the same means, for example, about 90% to about 110% of the measured synaptic plasticity in a normal individual or to that predicted from an animal model for a normal individual. In other embodiments, substantially the same means, for example, about 80% to about 120% of the measured synaptic plasticity in a normal individual or to that predicted from an animal model for a normal individual. In yet other embodiments, substantially the same means, for example, about 70% to about 130% of the synaptic plasticity in a normal individual or to that predicted from an animal model for a normal individual. As used herein, “partial normalization of aberrant synaptic plasticity” refers to any change in aberrant synaptic plasticity in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder that trends towards synaptic plasticity of a normal individual or to that predicted from an animal model for a normal individual. As used herein “partially normalized synaptic plasticity” or “partially normal synaptic plasticity” is, for example, ±about 25%, ±about 35%, ±about 45%, ±about 55%, ±about 65%, or ±about 75% of the synaptic plasticity of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant synaptic plasticity in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is lowering of aberrant synaptic plasticity where the aberrant synaptic plasticity is higher than the synaptic plasticity of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant synaptic plasticity in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is an increase in aberrant synaptic plasticity where the aberrant synaptic plasticity is lower than the synaptic plasticity of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of synaptic plasticity in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from an erratic (e.g., fluctuating, randomly increasing or decreasing) synaptic plasticity to a normal (e.g. stable) or partially normal (e.g., less fluctuating) synaptic plasticity compared to the synaptic plasticity of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of synaptic plasticity in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from a non-stabilizing synaptic plasticity to a normal (e.g., stable) or partially normal (e.g., partially stable) synaptic plasticity compared to the synaptic plasticity of a normal individual or to that predicted from an animal model for a normal individual.

As used herein, “normalization of aberrant baseline synaptic transmission” refers to a change in aberrant baseline synaptic transmission in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder to a level of baseline synaptic transmission that is substantially the same as the baseline synaptic transmission of a normal individual or to that predicted from an animal model for a normal individual. As used herein, substantially the same means, for example, about 90% to about 110% of the measured baseline synaptic transmission in a normal individual or to that predicted from an animal model for a normal individual. In other embodiments, substantially the same means, for example, about 80% to about 120% of the measured baseline synaptic transmission in a normal individual or to that predicted from an animal model for a normal individual. In yet other embodiments, substantially the same means, for example, about 70% to about 130% of the measured baseline synaptic transmission in a normal individual or to that predicted from an animal model for a normal individual. As used herein, “partial normalization of aberrant baseline synaptic transmission” refers to any change in aberrant baseline synaptic transmission in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder that trends towards baseline synaptic transmission of a normal individual or to that predicted from an animal model for a normal individual. As used herein “partially normalized baseline synaptic transmission” or “partially normal baseline synaptic transmission” is, for example, ±about 25%, ±about 35%, ±about 45%, ±about 55%, ±about 65%, or ±about 75% of the measured baseline synaptic transmission of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant baseline synaptic transmission in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is lowering of aberrant baseline synaptic transmission where the aberrant baseline synaptic transmission is higher than the baseline synaptic transmission of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant baseline synaptic transmission in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is an increase in aberrant baseline synaptic transmission where the aberrant baseline synaptic transmission is lower than the baseline synaptic transmission of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of baseline synaptic transmission in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from an erratic (e.g., fluctuating, randomly increasing or decreasing) baseline synaptic transmission to a normal (e.g. stable) or partially normal (e.g., less fluctuating) baseline synaptic transmission compared to the baseline synaptic transmission of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant baseline synaptic transmission in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from a non-stabilizing baseline synaptic transmission to a normal (e.g., stable) or partially normal (e.g., partially stable) baseline synaptic transmission compared to the baseline synaptic transmission of a normal individual or to that predicted from an animal model for a normal individual.

As used herein, “normalization of aberrant synaptic function” refers to a change in aberrant synaptic function in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder to a level of synaptic function that is substantially the same as the synaptic function of a normal individual or to that predicted from an animal model for a normal individual. As used herein, substantially the same means, for example, about 90% to about 110% of the synaptic function in a normal individual or to that predicted from an animal model for a normal individual. In other embodiments, substantially the same means, for example, about 80% to about 120% of the synaptic function in a normal individual or to that predicted from an animal model for a normal individual. In yet other embodiments, substantially the same means, for example, about 70% to about 130% of the synaptic function in a normal individual or to that predicted from an animal model for a normal individual. As used herein, “partial normalization of aberrant synaptic function” refers to any change in aberrant synaptic function in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder that trends towards synaptic function of a normal individual or to that predicted from an animal model for a normal individual. As used herein “partially normalized synaptic function” or “partially normal synaptic function” is, for example, ±about 25%, ±about 35%, ±about 45%, ±about 55%, ±about 65%, or ±about 75% of the measured synaptic function of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant synaptic function in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is lowering of aberrant synaptic function where the aberrant synaptic function is higher than the synaptic function of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant synaptic function in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is an increase in aberrant synaptic function where the aberrant synaptic function is lower than the synaptic function of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of synaptic function in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from an erratic (e.g., fluctuating, randomly increasing or decreasing) synaptic function to a normal (e.g. stable) or partially normal (e.g., less fluctuating) synaptic function compared to the synaptic function of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant synaptic function in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from a non-stabilizing synaptic function to a normal (e.g., stable) or partially normal (e.g., partially stable) synaptic function compared to the synaptic function of a normal individual or to that predicted from an animal model for a normal individual.

As used herein, “normalization of aberrant long term potentiation (LTP)” refers to a change in aberrant LTP in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder to a level of LTP that is substantially the same as the LTP of a normal individual or to that predicted from an animal model for a normal individual. As used herein, substantially the same means, for example, about 90% to about 110% of the LTP in a normal individual or to that predicted from an animal model for a normal individual. In other embodiments, substantially the same means, for example, about 80% to about 120% of the LTP in a normal individual or to that predicted from an animal model for a normal individual. In yet other embodiments, substantially the same means, for example, about 70% to about 130% of the LTP in a normal individual or to that predicted from an animal model for a normal individual. As used herein, “partial normalization of aberrant LTP” refers to any change in aberrant LTP in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder that trends towards LTP of a normal individual or to that predicted from an animal model for a normal individual. As used herein “partially normalized LTP” or “partially normal LTP” is, for example, ±about 25%, ±about 35%, ±about 45%, ±about 55%, ±about 65%, or ±about 75% of the measured LTP of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant LTP in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is lowering of aberrant LTP where the aberrant LTP is higher than the LTP of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant LTP in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is an increase in aberrant LTP where the aberrant LTP is lower than the LTP of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of LTP in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from an erratic (e.g., fluctuating, randomly increasing or decreasing) LTP to a normal (e.g. stable) or partially normal (e.g., less fluctuating) LTP compared to the LTP of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant LTP in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from a non-stabilizing LTP to a normal (e.g., stable) or partially normal (e.g., partially stable) LTP compared to the LTP of a normal individual or to that predicted from an animal model for a normal individual.

As used herein, “normalization of aberrant long term depression (LTD)” refers to a change in aberrant LTD in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder to a level of LTD that is substantially the same as the LTD of a normal individual or to that predicted from an animal model for a normal individual. As used herein, substantially the same means, for example, about 90% to about 110% of the LTD in a normal individual or to that predicted from an animal model for a normal individual. In other embodiments, substantially the same means, for example, about 80% to about 120% of the LTD in a normal individual or to that predicted from an animal model for a normal individual. In yet other embodiments, substantially the same means, for example, about 70% to about 130% of the LTD in a normal individual or to that predicted from an animal model for a normal individual. As used herein, “partial normalization of aberrant LTD” refers to any change in aberrant LTD in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder that trends towards LTD of a normal individual or to that predicted from an animal model for a normal individual. As used herein “partially normalized LTD” or “partially normal LTD” is, for example, ±about 25%, ±about 35%, ±about 45%, ±about 55%, ±about 65%, or ±about 75% of the measured LTD of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant LTD in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is lowering of aberrant LTD where the aberrant LTD is higher than the LTD of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant LTD in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is an increase in aberrant LTD where the aberrant LTD is lower than the LTD of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of LTD in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from an erratic (e.g., fluctuating, randomly increasing or decreasing) LTD to a normal (e.g. stable) or partially normal (e.g., less fluctuating) LTD compared to the LTD of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant LTD in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from a non-stabilizing LTD to a normal (e.g., stable) or partially normal (e.g., partially stable) LTD compared to the LTD of a normal individual or to that predicted from an animal model for a normal individual.

As used herein, “normalization of aberrant sensorimotor gating” refers to a change in aberrant sensorimotor gating in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder to a level of sensorimotor gating that is substantially the same as the sensorimotor gating of a normal individual or to that predicted from an animal model for a normal individual. As used herein, substantially the same means, for example, about 90% to about 110% of the sensorimotor gating in a normal individual or to that predicted from an animal model for a normal individual. In other embodiments, substantially the same means, for example, about 80% to about 120% of the sensorimotor gating in a normal individual or to that predicted from an animal model for a normal individual. In yet other embodiments, substantially the same means, for example, about 70% to about 130% of the sensorimotor gating in a normal individual or to that predicted from an animal model for a normal individual. As used herein, “partial normalization of aberrant sensorimotor gating” refers to any change in aberrant sensorimotor gating in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder that trends towards sensorimotor gating of a normal individual or to that predicted from an animal model for a normal individual. As used herein “partially normalized sensorimotor gating” or “partially normal sensorimotor gating” is, for example, ±about 25%, ±about 35%, ±about 45%, ±about 55%, ±about 65%, or ±about 75% of the measured sensorimotor gating of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant sensorimotor gating in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is lowering of aberrant sensorimotor gating where the aberrant sensorimotor gating is higher than the sensorimotor gating of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant sensorimotor gating in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is an increase in aberrant sensorimotor gating where the aberrant sensorimotor gating is lower than the sensorimotor gating of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of sensorimotor gating in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from an erratic (e.g., fluctuating, randomly increasing or decreasing) sensorimotor gating to a normal (e.g. stable) or partially normal (e.g., less fluctuating) sensorimotor gating compared to the sensorimotor gating of a normal individual or to that predicted from an animal model for a normal individual. In some embodiments, normalization or partial normalization of aberrant sensorimotor gating in an individual suffering from, suspected of having, or pre-disposed to a CNS disorder is a change from a non-stabilizing sensorimotor gating to a normal (e.g., stable) or partially normal (e.g., partially stable) sensorimotor gating compared to the sensorimotor gating of a normal individual or to that predicted from an animal model for a normal individual.

As used herein, “expression” of a nucleic acid sequence refers to one or more of the following events: (1) production of an RNA template from a DNA sequence (e.g., by transcription); (2) processing of an RNA transcript (e.g., by splicing, editing, 5′ cap formation, and/or 3′ end formation); (3) translation of an RNA into a polypeptide or protein; (4) post-translational modification of a polypeptide or protein.

As used herein the term “PAK polypeptide” or “PAK protein” or “PAK” refers to a protein that belongs in the family of p21-activated serine/threonine protein kinases. These include mammalian isoforms of PAK, e.g., the Group I PAK proteins (sometimes referred to as Group A PAK proteins), including PAK1, PAK2, PAK3, as well as the Group II PAK proteins (sometimes referred to as Group B PAK proteins), including PAK4, PAK5, and/or PAK6 Also included as PAK polypeptides or PAK proteins are lower eukaryotic isoforms, such as the yeast Step 20 (Leberter et al., 1992, EMBO J., 11:4805; incorporated herein by reference) and/or the Dictyostelium single-headed myosin I heavy chain kinases (Wu et al., 1996, J. Biol. Chem., 271:31787; incorporated herein by reference). Representative examples of PAK amino acid sequences include, but are not limited to, human PAK1 (GenBank Accession Number AAA65441), human PAK2 (GenBank Accession Number AAA65442), human PAK3 (GenBank Accession Number AAC36097), human PAK 4 (GenBank Accession Numbers NP_(—)005875 and CAA09820), human PAK5 (GenBank Accession Numbers CAC18720 and BAA94194), human PAK6 (GenBank Accession Numbers NP_(—)064553 and AAF82800), human PAK1 (GenBank Accession Number Q9P286), C. elegans PAK (GenBank Accession Number BAA11844), D. melanogaster PAK (GenBank Accession Number AAC47094), and rat PAK1 (GenBank Accession Number AAB95646). In some embodiments, a PAK polypeptide comprises an amino acid sequence that is at least 70% to 100% identical, e.g., at least 75%, 80%, 85%, 86%, 87%, 88%, 90%, 91%, 92%, 94%, 95%, 96%, 97%, 98%, or any other percent from about 70% to about 100% identical to sequences of GenBank Accession Numbers AAA65441, AAA65442, AAC36097, NP_(—)005875, CAA09820, CAC18720, BAA94194, NP_(—)064553, AAF82800, Q9P286, BAA11844, AAC47094, and/or AAB95646. In some embodiments, a Group I PAK polypeptide comprises an amino acid sequence that is at least 70% to 100% identical, e.g., at least 75%, 80%, 85%, 86%, 87%, 88%, 90%, 91%, 92%, 94%, 95%, 96%, 97%, 98%, or any other percent from about 70% to about 100% identical to sequences of GenBank Accession Numbers AAA65441, AAA65442, and/or AAC36097.

Representative examples of PAK genes encoding PAK proteins include, but are not limited to, human PAK1 (GenBank Accession Number U24152), human PAK2 (GenBank Accession Number U24153), human PAK3 (GenBank Accession Number AF068864), human PAK4 (GenBank Accession Number AJ011855), human PAK5 (GenBank Accession Number AB040812), and human PAK6 (GenBank Accession Number AF276893). In some embodiments, a PAK gene comprises a nucleotide sequence that is at least 70% to 100% identical, e.g., at least 75%, 80%, 85%, 86%, 87%, 88%, 90%, 91%, 92%, 94%, 95%, 96%, 97%, 98%, or any other percent from about 70% to about 100% identical to sequences of GenBank Accession Numbers U24152, U24153, AF068864, AJ011855, AB040812, and/or AF276893. In some embodiments, a Group I PAK gene comprises a nucleotide sequence that is at least 70% to 100% identical, e.g., at least 75%, 80%, 85%, 86%, 87%, 88%, 90%, 91%, 92%, 94%, 95%, 96%, 97%, 98%, or any other percent from about 70% to about 100% identical to sequences of GenBank Accession Numbers U24152, U24153, and/or AF068864.

To determine the percent homology of two amino acid sequences or of two nucleic acids, the sequences are aligned for optimal comparison purposes (e.g., gaps can be introduced in the sequence of a first amino acid or nucleic acid sequence for optimal alignment with a second amino or nucleic acid sequence). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in the first sequence is occupied by the same amino acid residue or nucleotide as the corresponding position in the second sequence, then the molecules are identical at that position. The percent homology between the two sequences is a function of the number of identical positions shared by the sequences (i.e., % identity=# of identical positions/total # of positions (e.g., overlapping positions)×100). In one embodiment the two sequences are the same length.

To determine percent homology between two sequences, the algorithm of Karlin and Altschul (1990) Proc. Natl. Acad. Sci. USA 87:2264-2268, modified as in Karlin and Altschul (1993) Proc. Natl. Acad. Sci. USA 90:5873-5877 is used. Such an algorithm is incorporated into the NBLAST and XBLAST programs of Altschul, et al. (1990) J. Mol. Biol. 215:403-410. BLAST nucleotide searches are performed with the NBLAST program, score=100, wordlength=12 to obtain nucleotide sequences homologous to a nucleic acid molecules described or disclose herein. BLAST protein searches are performed with the XBLAST program, score=50, wordlength=3. To obtain gapped alignments for comparison purposes, Gapped BLAST is utilized as described in Altschul et al. (1997) Nucleic Acids Res. 25:3389-3402. When utilizing BLAST and Gapped BLAST programs, the default parameters of the respective programs (e.g., XBLAST and NBLAST) are used. See the website of the National Center for Biotechnology Information for further details (on the world wide web at ncbi.nlm.nih.gov). Proteins suitable for use in the methods described herein also includes proteins having between 1 to 15 amino acid changes, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or 15 amino acid substitutions, deletions, or additions, compared to the amino acid sequence of any protein PAK inhibitor described herein. In other embodiments, the altered amino acid sequence is at least 75% identical, e.g., 77%, 80%, 82%, 85%, 88%, 90%, 92%, 95%, 97%, 98%, 99%, or 100% identical to the amino acid sequence of any protein PAK inhibitor described herein. Such sequence-variant proteins are suitable for the methods described herein as long as the altered amino acid sequence retains sufficient biological activity to be functional in the compositions and methods described herein. Where amino acid substitutions are made, the substitutions should be conservative amino acid substitutions. Among the common amino acids, for example, a “conservative amino acid substitution” is illustrated by a substitution among amino acids within each of the following groups: (1) glycine, alanine, valine, leucine, and isoleucine, (2) phenylalanine, tyrosine, and tryptophan, (3) serine and threonine, (4) aspartate and glutamate, (5) glutamine and asparagine, and (6) lysine, arginine and histidine. The BLOSUM62 table is an amino acid substitution matrix derived from about 2,000 local multiple alignments of protein sequence segments, representing highly conserved regions of more than 500 groups of related proteins (Henikoff et al (1992), Proc. Natl. Acad. Sci. USA, 89:10915-10919). Accordingly, the BLOSUM62 substitution frequencies are used to define conservative amino acid substitutions that may be introduced into the amino acid sequences described or described herein. Although it is possible to design amino acid substitutions based solely upon chemical properties (as discussed above), the language “conservative amino acid substitution” preferably refers to a substitution represented by a BLOSUM62 value of greater than −1. For example, an amino acid substitution is conservative if the substitution is characterized by a BLOSUM62 value of 0, 1, 2, or 3. According to this system, preferred conservative amino acid substitutions are characterized by a BLOSUM62 value of at least 1 (e.g., 1, 2 or 3), while more preferred conservative amino acid substitutions are characterized by a BLOSUM62 value of at least 2 (e.g., 2 or 3).

As used herein, the term “PAK activity,” unless otherwise specified, includes, but is not limited to, at least one of PAK protein-protein interactions, PAK phosphotransferase activity (intermolecular or intermolecular), translocation, etc of one or more PAK isoforms.

As used herein, a “PAK inhibitor” refers to any molecule, compound, or composition that directly or indirectly decreases the PAK activity. In some embodiments, PAK inhibitors inhibit, decrease, and/or abolish the level of a PAK mRNA and/or protein or the half-life of PAK mRNA and/or protein, such inhibitors are referred to as “clearance agents”. In some embodiments, a PAK inhibitor is a PAK antagonist that inhibits, decreases, and/or abolishes an activity of PAK. In some embodiments, a PAK inhibitor also disrupts, inhibits, or abolishes the interaction between PAK and its natural binding partners (e.g., a substrate for a PAK kinase, a Rac protein, a cdc42 protein, LIM kinase) or a protein that is a binding partner of PAK in a pathological condition, as measured using standard methods. In some embodiments, the PAK inhibitor is a Group I PAK inhibitor that inhibits, for example, one or more Group I PAK polypeptides, for example, PAK1, PAK2, and/or PAK3. In some embodiments, the PAK inhibitor is a PAK1 inhibitor. In some embodiments, the PAK inhibitor is a PAK2 inhibitor. In some embodiments, the PAK inhibitor is a PAK3 inhibitor. In some embodiments, the PAK inhibitor is a mixed PAK1/PAK3 inhibitor. In some embodiments, the PAK inhibitor inhibits all three Group I PAK isoforms (PAK1, PAK2 and PAK3) with equal or similar potency. In some embodiments, the PAK inhibitor is a Group II PAK inhibitor that inhibits one or more Group II PAK polypeptides, for example PAK4, PAK5, and/or PAK6. In some embodiments, the PAK inhibitor is a PAK4 inhibitor. In some embodiments, the PAK inhibitor is a PAK5 inhibitor. In some embodiments, the PAK inhibitor is a PAK6 inhibitor. In some embodiments, the PAK inhibitor is a PAK7 inhibitor. As used herein, a PAK5 polypeptide is substantially homologous to a PAK7 polypeptide.

In some embodiments, PAK inhibitors reduce, abolish, and/or remove the binding between PAK and at least one of its natural binding partners (e.g., Cdc42 or Rac). In some instances, binding between PAK and at least one of its natural binding partners is stronger in the absence of a PAK inhibitor (by e.g., 90%, 80%, 70%, 60%, 50%, 40%, 30% or 20%) than in the presence of a PAK inhibitor. In some embodiments, PAK inhibitors prevent, reduce, or abolish binding between PAK and a protein that abnormally accumulates or aggregates in cells or tissue in a disease state. In some instances, binding between PAK and at least one of the proteins that aggregates or accumulates in a cell or tissue is stronger in the absence of a PAK inhibitor (by e.g., 90%, 80%, 70%, 60%, 50%, 40%, 30% or 20%) than in the presence of an inhibitor.

An “individual” or an “individual,” as used herein, is a mammal. In some embodiments, an individual is an animal, for example, a rat, a mouse, a dog or a monkey. In some embodiments, an individual is a human patient. In some embodiments an “individual” or an “individual” is a human. In some embodiments, an individual suffers from a CNS disorder or is suspected to be suffering from a CNS disorder or is pre-disposed to a CNS disorder.

In some embodiments, a pharmacological composition comprising a PAK inhibitor is “administered peripherally” or “peripherally administered.” As used herein, these terms refer to any form of administration of an agent, e.g., a therapeutic agent, to an individual that is not direct administration to the CNS, i.e., that brings the agent in contact with the non-brain side of the blood-brain barrier. “Peripheral administration,” as used herein, includes intravenous, intra-arterial, subcutaneous, intramuscular, intraperitoneal, transdermal, by inhalation, transbuccal, intranasal, rectal, oral, parenteral, sublingual, or trans-nasal. In some embodiments, a PAK inhibitor is administered by an intracerebral route.

The terms “polypeptide,” and “protein” are used interchangeably herein to refer to a polymer of amino acid residues. That is, a description directed to a polypeptide applies equally to a description of a protein, and vice versa. The terms apply to naturally occurring amino acid polymers as well as amino acid polymers in which one or more amino acid residues is a non-naturally occurring amino acid, e.g., an amino acid analog. As used herein, the terms encompass amino acid chains of any length, including full length proteins (i.e., antigens), wherein the amino acid residues are linked by covalent peptide bonds.

The term “amino acid” refers to naturally occurring and non-naturally occurring amino acids, as well as amino acid analogs and amino acid mimetics that function in a manner similar to the naturally occurring amino acids. Naturally encoded amino acids are the 20 common amino acids (alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, and valine) and pyrolysine and selenocysteine. Amino acid analogs refers to compounds that have the same basic chemical structure as a naturally occurring amino acid, i.e., an α carbon that is bound to a hydrogen, a carboxyl group, an amino group, and an R group, such as, homoserine, norleucine, methionine sulfoxide, methionine methyl sulfonium. Such analogs have modified R groups (such as, norleucine) or modified peptide backbones, but retain the same basic chemical structure as a naturally occurring amino acid.

Amino acids may be referred to herein by either their commonly known three letter symbols or by the one-letter symbols recommended by the IUPAC-IUB Biochemical Nomenclature Commission. Nucleotides, likewise, may be referred to by their commonly accepted single-letter codes.

The term “nucleic acid” refers to deoxyribonucleotides, deoxyribonucleosides, ribonucleosides, or ribonucleotides and polymers thereof in either single- or double-stranded form. Unless specifically limited, the term encompasses nucleic acids containing known analogues of natural nucleotides which have similar binding properties as the reference nucleic acid and are metabolized in a manner similar to naturally occurring nucleotides. Unless specifically limited otherwise, the term also refers to oligonucleotide analogs including PNA (peptidonucleic acid), analogs of DNA used in antisense technology (phosphorothioates, phosphoroamidates, and the like). Unless otherwise indicated, a particular nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (including but not limited to, degenerate codon substitutions) and complementary sequences as well as the sequence explicitly indicated. Specifically, degenerate codon substitutions may be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed-base and/or deoxyinosine residues (Batzer et al., Nucleic Acid Res. 19:5081 (1991); Ohtsuka et al., J. Biol. Chem. 260:2605-2608 (1985); and Cassol et al. (1992); Rossolini et al., Mol. Cell. Probes 8:91-98 (1994)).

The terms “isolated” and “purified” refer to a material that is substantially or essentially removed from or concentrated in its natural environment. For example, an isolated nucleic acid is one that is separated from the nucleic acids that normally flank it or other nucleic acids or components (proteins, lipids, etc.) in a sample. In another example, a polypeptide is purified if it is substantially removed from or concentrated in its natural environment. Methods for purification and isolation of nucleic acids and proteins are documented methodologies.

The term “antibody” describes an immunoglobulin whether natural or partly or wholly synthetically produced. The term also covers any polypeptide or protein having a binding domain which is, or is homologous to, an antigen-binding domain. CDR grafted antibodies are also contemplated by this term.

The term antibody as used herein will also be understood to mean one or more fragments of an antibody that retain the ability to specifically bind to an antigen, (see generally, Holliger et al., Nature Biotech. 23 (9) 1126-1129 (2005)). Non-limiting examples of such antibodies include (i) a Fab fragment, a monovalent fragment consisting of the VL, VH, CL and CH1 domains; (ii) a F(ab′)2 fragment, a bivalent fragment comprising two Fab fragments linked by a disulfide bridge at the hinge region; (iii) a Fd fragment consisting of the VH and CH1 domains; (iv) a Fv fragment consisting of the VL and VH domains of a single arm of an antibody, (v) a dAb fragment (Ward et al., (1989) Nature 341:544 546), which consists of a VH domain; and (vi) an isolated complementarity determining region (CDR). Furthermore, although the two domains of the Fv fragment, VL and VH, are coded for by separate genes, they are optionally joined, using recombinant methods, by a synthetic linker that enables them to be made as a single protein chain in which the VL and VH regions pair to form monovalent molecules (known as single chain Fv (scFv); see e.g., Bird et al. (1988) Science 242:423 426; and Huston et al. (1988) Proc. Natl. Acad. Sci. USA 85:5879 5883; and Osbourn et al. (1998) Nat. Biotechnol. 16:778). Such single chain antibodies are also intended to be encompassed within the term antibody. Any VH and VL sequences of specific scFv is optionally linked to human immunoglobulin constant region cDNA or genomic sequences, in order to generate expression vectors encoding complete IgG molecules or other isotypes. VH and VL are also optionally used in the generation of Fab, Fv or other fragments of immunoglobulins using either protein chemistry or recombinant DNA technology. Other forms of single chain antibodies, such as diabodies are also encompassed.

“F(ab′)2” and “Fab′” moieties are optionally produced by treating immunoglobulin (monoclonal antibody) with a protease such as pepsin and papain, and includes an antibody fragment generated by digesting immunoglobulin near the disulfide bonds existing between the hinge regions in each of the two H chains. For example, papain cleaves IgG upstream of the disulfide bonds existing between the hinge regions in each of the two H chains to generate two homologous antibody fragments in which an L chain composed of VL (L chain variable region) and CL (L chain constant region), and an H chain fragment composed of VH (H chain variable region) and CHγ1 (γ1 region in the constant region of H chain) are connected at their C terminal regions through a disulfide bond. Each of these two homologous antibody fragments is called Fab′. Pepsin also cleaves IgG downstream of the disulfide bonds existing between the hinge regions in each of the two H chains to generate an antibody fragment slightly larger than the fragment in which the two above-mentioned Fab′ are connected at the hinge region. This antibody fragment is called F(ab′)2.

The Fab fragment also contains the constant domain of the light chain and the first constant domain (CH1) of the heavy chain. Fab′ fragments differ from Fab fragments by the addition of a few residues at the carboxyl terminus of the heavy chain CH1 domain including one or more cysteine(s) from the antibody hinge region. Fab′-SH is the designation herein for Fab′ in which the cysteine residue(s) of the constant domains bear a free thiol group. F(ab′)2 antibody fragments originally were produced as pairs of Fab′ fragments which have hinge cysteines between them. Other chemical couplings of antibody fragments are documented.

“Fv” is the minimum antibody fragment which contains a complete antigen-recognition and antigen-binding site. This region consists of a dimer of one heavy chain and one light chain variable domain in tight, non-covalent association. It is in this configuration that the three hypervariable regions of each variable domain interact to define an antigen-binding site on the surface of the VH-VL dimer. Collectively, the six hypervariable regions confer antigen-binding specificity to the antibody. However, even a single variable domain (or half of an Fv comprising only three hypervariable regions specific for an antigen) has the ability to recognize and bind antigen, although at a lower affinity than the entire binding site.

“Single-chain Fv” or “sFv” antibody fragments comprise a VH, a VL, or both a VH and VL domain of an antibody, wherein both domains are present in a single polypeptide chain. In some embodiments, the Fv polypeptide further comprises a polypeptide linker between the VH and VL domains which enables the sFv to form the desired structure for antigen binding. For a review of sFv see, e.g., Pluckthun in The Pharmacology of Monoclonal Antibodies, Vol. 113, Rosenburg and Moore eds. Springer-Verlag, New York, pp. 269 315 (1994).

A “chimeric” antibody includes an antibody derived from a combination of different mammals. The mammal is, for example, a rabbit, a mouse, a rat, a goat, or a human. The combination of different mammals includes combinations of fragments from human and mouse sources.

In some embodiments, an antibody described or described herein is a monoclonal antibody (MAb), typically a chimeric human-mouse antibody derived by humanization of a mouse monoclonal antibody. Such antibodies are obtained from, e.g., transgenic mice that have been “engineered” to produce specific human antibodies in response to antigenic challenge. In this technique, elements of the human heavy and light chain locus are introduced into strains of mice derived from embryonic stem cell lines that contain targeted disruptions of the endogenous heavy chain and light chain loci. In some embodiments, the transgenic mice synthesize human antibodies specific for human antigens, and the mice are used to produce human antibody-secreting hybridomas.

The term “optionally substituted” or “substituted” means that the referenced group substituted with one or more additional group(s). In certain embodiments, the one or more additional group(s) are individually and independently selected from amide, ester, alkyl, cycloalkyl, heteroalkyl, aryl, heteroaryl, heteroalicyclic, hydroxy, alkoxy, aryloxy, alkylthio, arylthio, alkylsulfoxide, arylsulfoxide, ester, alkylsulfone, arylsulfone, cyano, halogen, alkoyl, alkoyloxo, isocyanato, thiocyanato, isothiocyanato, nitro, haloalkyl, haloalkoxy, fluoroalkyl, amino, alkyl-amino, dialkyl-amino, amido. In one embodiment, the referenced group is substituted with one or more halogen. In another embodiment, the referenced group is substituted with one or more alkyl.

An “alkyl” group refers to an aliphatic hydrocarbon group. Reference to an alkyl group includes “saturated alkyl” and/or “unsaturated alkyl”. The alkyl group, whether saturated or unsaturated, includes branched, straight chain, or cyclic groups. By way of example only, alkyl includes methyl, ethyl, propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl, t-butyl, pentyl, iso-pentyl, neo-pentyl, and hexyl. In some embodiments, alkyl groups include, but are in no way limited to, methyl, ethyl, propyl, isopropyl, butyl, isobutyl, tertiary butyl, pentyl, hexyl, ethenyl, propenyl, butenyl, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, and the like. A “lower alkyl” is a C₁-C₆ alkyl. A “heteroalkyl” group substitutes any one of the carbons of the alkyl group with a heteroatom having the appropriate number of hydrogen atoms attached (e.g., a CH₂ group to an NH group or an O group).

An “alkoxy” group refers to a (alkyl)O— group, where alkyl is as defined herein.

The term “alkylamine” refers to the —N(alkyl)_(x)H_(y) group, wherein alkyl is as defined herein and x and y are selected from the group x=1, y=1 and x=2, y=0. When x=2, the alkyl groups, taken together with the nitrogen to which they are attached, optionally form a cyclic ring system.

An “amide” is a chemical moiety with formula C(O)NHR or NHC(O)R, where R is selected from alkyl, cycloalkyl, aryl, heteroaryl (bonded through a ring carbon) and heteroalicyclic (bonded through a ring carbon).

The term “ester” refers to a chemical moiety with formula —C(═O)OR, where R is selected from the group consisting of alkyl, cycloalkyl, aryl, heteroaryl and heteroalicyclic.

As used herein, the term “aryl” refers to an aromatic ring wherein each of the atoms forming the ring is a carbon atom. Aryl rings described herein include rings having five, six, seven, eight, nine, or more than nine carbon atoms. Aryl groups are optionally substituted. Examples of aryl groups include, but are not limited to phenyl, and naphthalenyl.

The term “cycloalkyl” refers to a monocyclic or polycyclic non-aromatic radical, wherein each of the atoms forming the ring (i.e. skeletal atoms) is a carbon atom. In various embodiments, cycloalkyls are saturated, or partially unsaturated. In some embodiments, cycloalkyls are fused with an aromatic ring. Cycloalkyl groups include groups having from 3 to 10 ring atoms. Illustrative examples of cycloalkyl groups include, but are not limited to, the following moieties:

and the like. Monocyclic cycloalkyls include, but are not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl. Dicylclic cycloalkyls include, but are not limited to tetrahydronaphthyl, indanyl, tetrahydropentalene or the like. Polycyclic cycloalkyls include adamantane, norbornane or the like. The term cycloalkyl includes “unsaturated nonaromatic carbocyclyl” or “nonaromatic unsaturated carbocyclyl” groups both of which refer to a nonaromatic carbocycle, as defined herein, that contains at least one carbon carbon double bond or one carbon carbon triple bond.

The term “heterocyclo” refers to heteroaromatic and heteroalicyclic groups containing one to four ring heteroatoms each selected from O, S and N. In certain instances, each heterocyclic group has from 4 to 10 atoms in its ring system, and with the proviso that the ring of said group does not contain two adjacent O or S atoms. Non-aromatic heterocyclic groups include groups having 3 atoms in their ring system, but aromatic heterocyclic groups must have at least 5 atoms in their ring system. The heterocyclic groups include benzo-fused ring systems. An example of a 3-membered heterocyclic group is aziridinyl (derived from aziridine). An example of a 4-membered heterocyclic group is azetidinyl (derived from azetidine). An example of a 5-membered heterocyclic group is thiazolyl. An example of a 6-membered heterocyclic group is pyridyl, and an example of a 10-membered heterocyclic group is quinolinyl. Examples of non-aromatic heterocyclic groups are pyrrolidinyl, tetrahydropyranyl, dihydrofuranyl, tetrahydrothienyl, tetrahydropyranyl, dihydropyranyl, tetrahydrothiopyranyl, piperidino, morpholino, thiomorpholino, thioxanyl, piperazinyl, aziridinyl, azetidinyl, oxetanyl, thietanyl, homopiperidinyl, oxepanyl, thiepanyl, oxazepinyl, diazepinyl, thiazepinyl, 1,2,3,6-tetrahydropyridinyl, 2-pyrrolinyl, 3-pyrrolinyl, indolinyl, 2H-pyranyl, 4H-pyranyl, dioxanyl, 1,3-dioxolanyl, pyrazolinyl, dithianyl, dithiolanyl, dihydropyranyl, dihydrothienyl, dihydrofuranyl, pyrazolidinyl, imidazolinyl, imidazolidinyl, 3-azabicyclo[3.1.0]hexanyl, 3-azabicyclo[4.1.0]heptanyl, 3H-indolyl and quinolizinyl. Examples of aromatic heterocyclic groups are pyridinyl, imidazolyl, pyrimidinyl, pyrazolyl, triazolyl, pyrazinyl, tetrazolyl, furyl, thienyl, isoxazolyl, thiazolyl, oxazolyl, isothiazolyl, pyrrolyl, quinolinyl, isoquinolinyl, indolyl, benzimidazolyl, benzofuranyl, cinnolinyl, indazolyl, indolizinyl, phthalazinyl, pyridazinyl, triazinyl, isoindolyl, pteridinyl, purinyl, oxadiazolyl, thiadiazolyl, furazanyl, benzofurazanyl, benzothiophenyl, benzothiazolyl, benzoxazolyl, quinazolinyl, quinoxalinyl, naphthyridinyl, and furopyridinyl.

The terms “heteroaryl” or, alternatively, “heteroaromatic” refers to an aryl group that includes one or more ring heteroatoms selected from nitrogen, oxygen and sulfur. An N-containing “heteroaromatic” or “heteroaryl” moiety refers to an aromatic group in which at least one of the skeletal atoms of the ring is a nitrogen atom. In certain embodiments, heteroaryl groups are monocyclic or polycyclic. Examples of monocyclic heteroaryl groups include and are not limited to:

Examples of bicyclic heteroaryl groups include and are not limited to:

or the like.

A “heteroalicyclic” group or “heterocyclo” group or “heterocycloalkyl” group or “heterocyclyl” group refers to a cycloalkyl group, wherein at least one skeletal ring atom is a heteroatom selected from nitrogen, oxygen and sulfur. In various embodiments, heterocycloalkyls are saturated, or partially unsaturated. In some embodiments, the radicals are fused with an aryl or heteroaryl. Example of saturated heterocyloalkyl groups include

Examples of partially unsaturated heterocyclyl or heterocycloalkyl groups include

Other illustrative examples of heterocyclo or heterocycloalkyl groups, also referred to as non-aromatic heterocycles, include:

or the like.

The term heteroalicyclic also includes all ring forms of the carbohydrates, including but not limited to the monosaccharides, the disaccharides and the oligosaccharides.

The term “halo” or, alternatively, “halogen” means fluoro, chloro, bromo and iodo.

The terms “haloalkyl,” and “haloalkoxy” include alkyl and alkoxy structures that are substituted with one or more halogens. In embodiments, where more than one halogen is included in the group, the halogens are the same or they are different. The terms “fluoroalkyl” and “fluoroalkoxy” include haloalkyl and haloalkoxy groups, respectively, in which the halo is fluorine.

The term “heteroalkyl” include optionally substituted alkyl, alkenyl and alkynyl radicals which have one or more skeletal chain atoms selected from an atom other than carbon, e.g., oxygen, nitrogen, sulfur, phosphorus, silicon, or combinations thereof. In certain embodiments, the heteroatom(s) is placed at any interior position of the heteroalkyl group. Examples include, but are not limited to, —CH₂—O—CH₃, —CH₂—CH₂—O—CH₃, —CH₂—NH—CH₃, —CH₂—CH₂—NH—CH₃, —CH₂—N(CH₃)—CH₃, —CH₂—CH₂—NH—CH₃, —CH₂—CH₂—N(CH₃)—CH₃, —CH₂—S—CH₂—CH₃, —CH₂—CH₂, —S(O)—CH₃, —CH₂—CH₂, —S(O)₂—CH₃, —CH═CH—O—CH₃, —Si(CH₃)₃, —CH₂—CH═N—OCH₃, and —CH═CH—N(CH₃)—CH₃. In some embodiments, up to two heteroatoms are consecutive, such as, by way of example, —CH₂—NH—OCH₃ and —CH₂—O—Si(CH₃)₃.

A “cyano” group refers to a CN group.

An “isocyanato” group refers to a NCO group.

A “thiocyanato” group refers to a CNS group.

An “isothiocyanato” group refers to a NCS group.

“Alkoyloxy” refers to a RC(═O)O— group.

“Alkoyl” refers to a RC(═O)— group.

Synthesis of Compounds

In some embodiments, compounds of Formula I, II, III, IV, V, Va, or Vb are synthesized according to procedures described in Scheme 1 and in the Examples section.

Generally, compounds of Formula IX described herein are synthesized by conversion of (methylthio)-pyridopyrimidinone, I, to its bromo derivative II. Substitution at the NH of the core, for example by alkylation with a halogen containing Q forms substituted compound III. Oxidation of the sulfanyl compound III using an oxidizing agent such as for example, chloroperbenzoic acid gives sulfinyl compound IV. Addition of the B-ring (V) results in compounds of Formula VI. Addition of the T ring (VIII) where M represents a group such a boronic acid, boronic ester, alkyl tin, zinc atom or other similar moieties generates compound IX. Alternatively, VI can be converted to its boronic acid VIII and ring T (X) can be attached via a halogen atom to generate IX. The procedures described herein are given merely as an example and should in no way limit the methods of making the compounds described herein.

In other embodiments, compounds described herein are synthesized according to the procedures described in Scheme 2 and in the Examples section.

Generally, compounds of Formula VII described herein are synthesized by conversion of (methylthio)-4-Q-substituted-amino-pyrimidine-carbaldehyde, I, to the 4-bromo-2-chlorophenyl-pyrido-pyrimidinone, II. Oxidation of the sulfanyl compound II using an oxidizing agent such as for example, chloroperbenzoic acid gives sulfinyl compound III. Addition of the B-ring (IV) results in compounds of Formula V. Addition of the R₁ substituent where M represents a group such a boronic acid, boronic ester, alkyl tin, zinc atom or other similar moieties generates compound VII. The procedures described herein are given merely as an example and should in no way limit the methods of making the compounds described herein.

Methods

Provided herein are methods for treating CNS disorders comprising administration of a therapeutically effective amount of a p21-activated kinase inhibitor (e.g., a compound of Formula I-XV) to an individual in need thereof. In some embodiments of the methods provided herein, administration of a p21-activated kinase inhibitor alleviates or reverses one or more behavioral symptoms (e.g., social withdrawal, depersonalization, loss of appetite, loss of hygiene, delusions, hallucinations, depression, blunted affect, avolition, anhedonia, alogia, the sense of being controlled by outside forces or the like) of the CNS disorder (e.g. negative symptoms of schizophrenia). In some embodiments of the methods provided herein, administration of a p21-activated kinase inhibitor (e.g., a compound of Formula I-XV) alleviates or reverses one or more negative symptoms and/or cognition impairment associated with a CNS disorder (e.g., impairment in executive function, comprehension, inference, decision-making, planning, learning or memory associated with schizophrenia, Alzheimer's disease, FXS, autism or the like).

Also provided herein are methods for modulation of dendritic spine morphology and/or synaptic function comprising administering to an individual in need thereof (e.g., an individual suffering from or suspected of having schizophrenia, Parkinson's disease, Alzheimer's disease, epilepsy or the like) a therapeutically effective amount of a PAK inhibitor (e.g., a compound of Formula I-XV). In some embodiments, modulation of dendritic spine morphology and/or synaptic function alleviates or reverses negative symptoms and/or cognitive impairment associated with a CNS disorder. In some embodiments, modulation of dendritic spine morphology and/or synaptic function halts or delays further deterioration of symptoms associated with a CNS disorder (e.g., progression of cognitive impairments and/or loss of bodily functions). In some embodiments, modulation of dendritic spine morphology and/or synaptic function stabilizes or reverses symptoms of disease (e.g., reduces frequency of epileptic seizures, stabilizes mild cognitive impairment and prevents progression to early dementia). In some embodiments of the methods provided herein, administration of a p21-activated kinase inhibitor halts or delays progressive loss of memory and/or cognition associated with a CNS disorder (e.g., Alzheimer's disease).

Provided herein are methods for modulation of synaptic function or synaptic plasticity comprising administering to an individual in need thereof (e.g., an individual suffering from or suspected of having any CNS disorder described herein) a therapeutically effective amount of a PAK inhibitor (e.g., a compound of Formula I-XV). Modulation of synaptic function or plasticity includes, for example, alleviation or reversal of defects in LTP, LTD or the like.

Defects in LTP include, for example, an increase in LTP or a decrease in LTP in any region of the brain in an individual suffering from or suspected of having a CNS disorder. Defects in LTD include for example a decrease in LTD or an increase in LTD in any region of the brain (e.g., the temporal lobe, parietal lobe, the frontal cortex, the cingulate gyrus, the prefrontal cortex, the cortex, or the hippocampus or any other region in the brain or a combination thereof) in an individual suffering from or suspected of having a CNS disorder.

In some embodiments of the methods, administration of a PAK inhibitor (e.g., a compound of Formula I-XV) modulates synaptic function (e.g., synaptic transmission and/or plasticity) by increasing long term potentiation (LTP) in an individual suffering from or suspected of having a CNS disorder. In some embodiments of the methods described herein, administration of a PAK inhibitor (e.g., a compound of Formula I-XV) to an individual in need thereof modulates synaptic function (e.g., synaptic transmission and/or plasticity) by increasing long term potentiation (LTP) in the prefrontal cortex, or the cortex, or the hippocampus or any other region in the brain or a combination thereof. In some embodiments of the methods described herein, administration of a PAK inhibitor modulates synaptic function (e.g., synaptic transmission and/or plasticity) by decreasing long term depression (LTD) in an individual suffering from or suspected of having a CNS disorder. In some embodiments of the methods described herein, administration of a PAK inhibitor to an individual in need thereof modulates synaptic function (e.g., synaptic transmission and/or plasticity) by decreasing long term depression (LTD) in the temporal lobe, parietal lobe, the frontal cortex, the cingulate gyrus, the prefrontal cortex, the cortex, or the hippocampus or any other region in the brain or a combination thereof.

In some embodiments of the methods described herein, administration of a PAK inhibitor reverses defects in synaptic function (i.e. synaptic transmission and/or synaptic plasticity, induced by soluble Abeta dimers or oligomers. In some embodiments of the methods described herein, administration of a PAK inhibitor reverses defects in synaptic function (i.e. synaptic transmission and/or synaptic plasticity, induced by insoluble Abeta oligomers and/or Abeta-containing plaques.

Provided herein are methods for stabilization of synaptic plasticity comprising administering to an individual in need thereof (e.g., an individual suffering from or suspected of having a CNS disorder) a therapeutically effective amount of a PAK inhibitor (e.g., a compound of Formula I-XV). In some embodiments of the methods described herein, administration of a PAK inhibitor stabilizes LTP or LTD following induction (e.g., by theta-burst stimulation, high-frequency stimulation for LTP, low-frequency (e.g., 1 Hz) stimulation for LTD).

Provided herein are methods for stabilization of synaptic transmission comprising administering to an individual in need thereof (e.g., an individual suffering from or suspected of having a CNS disorder) a therapeutically effective amount of a PAK inhibitor (e.g., a compound of Formula I-XV). In some embodiments of the methods described herein, administration of a PAK inhibitor stabilizes LTP or LTD following induction (e.g., by theta-burst stimulation, high-frequency stimulation for LTP, low-frequency (e.g., 1 Hz) stimulation for LTD).

Also provided herein are methods for alleviation or reversal of cortical hypofrontality during performance of a cognitive task comprising administering to an individual in need thereof (e.g., an individual suffering from or suspected of having a CNS disorder) a therapeutically effective amount of a PAK inhibitor (e.g., a compound of Formula I-XV). In some embodiments of the methods described herein, administration of a PAK inhibitor to an individual suffering from or suspected of having a CNS disorder alleviates deficits in the frontal cortex, for example deficits in frontal cortical activation, during the performance of a cognitive task (e.g., a Wisconsin Card Sort test, Mini-Mental State Examination (MMSE), MATRICS cognitive battery, BACS score, Alzheimer's disease Assessment Scale-Cognitive Subscale (ADAS-Cog), Alzheimer's disease Assessment Scale-Behavioral Subscale (ADAS-Behav), Hopkins Verbal Learning Test-Revised or the like) and improves cognition scores of the individual.

Provided herein are methods for reversing abnormalities in dendritic spine morphology or synaptic function that are caused by mutations in high-risk genes (e.g. mutations in Amyloid Precursor Protein (APP), mutations in presenilin 1 and 2, the epsilon4 allele, the 91 bp allele in the telomeric region of 12q, Apolipoprotein E-4 (APOE4) gene, SORL1 gene, reelin gene, DISC1 gene, or any other high-risk allele) comprising administering to an individual in need thereof a therapeutically effective amount of a PAK inhibitor (e.g., a compound of Formula I-XV). In some embodiments of the methods described herein, prophylactic administration of a PAK inhibitor to an individual at a high risk for developing a CNS disorder (e.g., a mutation in a DISC1 gene pre-disposes the individual to schizophrenia, a mutation in an APOE4 gene pre-disposes the individual to Alzheimer's disease) reverses abnormalities in dendritic spine morphology and/or synaptic function and prevents development of the CNS disorder.

Provided herein are methods for stabilizing, reducing or reversing abnormalities in dendritic spine morphology or synaptic function that are caused by increased activation of PAK at the synapse, comprising administration of a therapeutically effective amount of a PAK inhibitor (e.g., a compound of Formula I-XV) to an individual in need thereof (e.g., an individual suffering from or suspected of having a CNS disorder). In some embodiments of the methods described herein, increased activation of PAK at the synapse is caused by Abeta. In some instances, increased activation of PAK at the synapse is caused by redistribution of PAK from the cytosol to the synapse. In some embodiments of the methods described herein, administration of a therapeutically effective amount of a PAK inhibitor (e.g., a compound of Formula I-XV) to an individual in need thereof (e.g., an individual suffering from or suspected of having a CNS disorder) reduces or prevents redistribution of PAK from the cytosol to the synapse in neurons, thereby stabilizing, reducing or reversing abnormalities in dendritic spine morphology or synaptic function that are caused by increased activation of PAK at the synapse.

Provided herein are methods for delaying the onset of a CNS disorder comprising administering to an individual in need thereof (e.g., an individual with a high-risk allele for a NC) a therapeutically effective amount of a PAK inhibitor (e.g., a compound of Formula I-XV). Provided herein are methods for delaying the loss of dendritic spine density comprising administering to an individual in need thereof (e.g., an individual with a high-risk allele for a CNS disorder) a therapeutically effective amount of a PAK inhibitor. Provided herein are methods for modulation of spine density, shape, spine length, spine head volume, or spine neck diameter or the like comprising administering to an individual in need thereof (e.g., an individual suffering from or suspected of having a CNS disorder) a therapeutically effective amount of a PAK inhibitor (e.g., a compound of Formula I-XV). Provided herein are methods of modulating the ratio of mature dendritic spines to immature dendritic spines comprising administering to an individual in need thereof (e.g., an individual suffering from or suspected of having a CNS disorder) a therapeutically effective amount of a PAK inhibitor. Provided herein are methods of modulating the ratio of dendritic spines head volume to dendritic spines length comprising administering to an individual in need thereof (e.g., an individual suffering from or suspected of having a CNS disorder) a therapeutically effective amount of a PAK inhibitor (e.g., a compound of Formula I-XV).

In some embodiments of the methods described herein, administration of a PAK inhibitor (e.g., a maintenance dose of a PAK inhibitor) reduces the incidence of recurrence of one or more symptoms or pathologies in an individual (e.g., recurrence of psychotic episodes, epileptic seizures or the like). In some embodiments of the methods described herein, administration of a PAK inhibitor causes substantially complete inhibition of PAK and restores dendritic spine morphology and/or synaptic function to normal levels. In some embodiments of the methods described herein, administration of a PAK inhibitor causes partial inhibition of PAK and restores dendritic spine morphology and/or synaptic function to normal levels.

Provided herein are methods for stabilizing, reducing or reversing neuronal withering and/or atrophy or nervous tissue and/or degeneration of nervous tissue that is associated with a CNS disorder. In some embodiments of the methods described herein, administration of a PAK inhibitor to an individual suffering from or suspected of having a CNS disorder (e.g., Alzheimer's disease, Parkinson's disease or the like) stabilizes, alleviates or reverses neuronal withering and/or atrophy and/or degeneration in the temporal lobe, parietal lobe, the frontal cortex, the cingulate gyrus or the like. In some embodiments of the methods described herein, administration of a PAK inhibitor to an individual suffering from or suspected of having a CNS disorder stabilizes, reduces or reverses deficits in memory and/or cognition and/or control of bodily functions.

In some instances, a CNS disorder is associated with a decrease in dendritic spine density. In some embodiments of the methods described herein, administration of a PAK inhibitor increases dendritic spine density. In some instances, a CNS disorder is associated with an increase in dendritic spine length. In some embodiments of the methods described herein, administration of a PAK inhibitor decreases dendritic spine length. In some instances, a CNS disorder is associated with a decrease in dendritic spine neck diameter. In some embodiments of the methods described herein, administration of a PAK inhibitor increases dendritic spine neck diameter. In some instances, a CNS disorder is associated with a decrease in dendritic spine head diameter and/or dendritic spine head surface area and/or dendritic spine head volume. In some embodiments of the methods described herein, administration of a PAK inhibitor increases dendritic spine head diameter and/or dendritic spine head volume and/or dendritic spine head surface area.

In some instances, a CNS disorder is associated with an increase in immature spines and a decrease in mature spines. In some embodiments of the methods described herein, administration of a PAK inhibitor modulates the ratio of immature spines to mature spines. In some instances, a CNS disorder is associated with an increase in stubby spines and a decrease in mushroom-shaped spines. In some embodiments of the methods described herein, administration of a PAK inhibitor modulates the ratio of stubby spines to mushroom-shaped spines.

In some embodiments of the methods described herein, administration of a PAK inhibitor modulates a spine:head ratio, e.g., ratio of the volume of the spine to the volume of the head, ratio of the length of a spine to the head diameter of the spine, ratio of the surface area of a spine to the surface area of the head of a spine, or the like, compared to a spine:head ratio in the absence of a PAK inhibitor. In certain embodiments, a PAK inhibitor suitable for the methods described herein modulates the volume of the spine head, the width of the spine head, the surface area of the spine head, the length of the spine shaft, the diameter of the spine shaft, or a combination thereof. In some embodiments, provided herein is a method of modulating the volume of a spine head, the width of a spine head, the surface area of a spine head, the length of a spine shaft, the diameter of a spine shaft, or a combination thereof, by contacting a neuron comprising the dendritic spine with an effective amount of a PAK inhibitor described herein. In specific embodiments, the neuron is contacted with the PAK inhibitor in vivo.

Also described herein are methods for treating cancer in a subject comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula I-XV. As used herein, “cancer” includes any malignant growth or tumor caused by abnormal and uncontrolled cell division. Examples of cancers include pancreatic cancer, gastrointestinal stromal tumors, lung cancer, stomach cancer, brain cancer, kidney cancer, breast cancer, head and neck cancer, myeloma, leukemia, lymphoma, adenocarcinoma, melanoma or the like.

In one embodiment is a method for treating cancer in a subject comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula I-XV wherein the cancer is selected from ovarian, breast, colon, brain, CML, renal cell carcinoma, gastric, leukemia, NSCLC, CNS, melanoma, prostate, T-cell lymphoma, heptocellular, bladder, glioblastoma, mesothelioma, neuroma, and meningioma. In one embodiment, the breast cancer is tamoxifen-resistant or intolerant breast cancer. In another embodiment, the CML is imatinib resistant or intolerant CML.

In one embodiment, is a method for modulating a p21 activated kinase comprising contacting a compound of Formula I-XV with a p21 activated kinase such that PAK expression or activation has been altered. PAK kinases have been identified as key regulators of cancer-cell signaling networks where they regulate essential biological processes. These processes include cytoskeletal dynamics, energy homeostasis, cell survival, differentiation, anchorage-independent growth, mitosis, and hormone dependence. Dysregulation of these processes by alterations in PAK expression or activation have been reported in numerous human cancers. See, e.g., Kumar R, Gururaj A E, Barnes C J, p21-activated kinases in cancer, Nat Rev Cancer, 2006; 6: 459-471, which is incorporated by reference herein to the extent it is relevant.

In another embodiment is a method for treating cancer in a subject comprising administering to a subject in need thereof a therapeutically effective amount of a compound of Formula I-XV wherein the cancer is selected from pancreatic cancer, gastrointestinal stromal tumors, lung cancer, stomach cancer, brain cancer, kidney cancer, breast cancer, head and neck cancer, myeloma, leukemia, lymphoma, adenocarcinoma, bone cancer, cutaneous or intraocular melanoma, uterine cancer, ovarian cancer, rectal cancer, cancer of the anal region, stomach cancer, colon cancer, carcinoma of the fallopian tubes, carcinoma of the endometrium, carcinoma of the cervix, carcinoma of the vagina, carcinoma of the vulva, Hodgkin's Disease, cancer of the esophagus, cancer of the small intestine, cancer of the endocrine system, cancer of the thyroid gland, cancer of the parathyroid gland, cancer of the adrenal gland, sarcoma of soft tissue, cancer of the urethra, cancer of the penis, prostate cancer, lymphocytic lymphomas, cancer of the bladder, renal cell carcinoma, carcinoma of the renal pelvis, neoplasms of the central nervous system (CNS), primary CNS lymphoma, spinal axis tumors, brain stem glioma, pituitary adenoma, or a combination of one or more of the foregoing cancers.

In certain embodiments, a compound or a composition comprising a compound described herein is administered for prophylactic and/or therapeutic treatments. In therapeutic applications, the compositions are administered to an individual already suffering from a disease or condition, in an amount sufficient to cure or at least partially arrest the symptoms of the disease or condition. In various instances, amounts effective for this use depend on the severity and course of the disease or condition, previous therapy, an individual's health status, weight, and response to the drugs, and the judgment of the treating physician.

In some embodiments, a composition containing a therapeutically effective amount of a PAK inhibitor is administered prophylactically to an individual that while not overtly manifesting symptoms of a CNS disorder has been identified as having a high risk of developing a CNS disorder, e.g., an individual is identified as being a carrier of a mutation or polymorphism associated with a higher risk to develop a CNS disorder (see, e.g., Hall et al (2006), Nat. Neurosci., 9(12):1477-8), or an individual that is from a family that has a high incidence of CNS disorders. In some embodiments, MRI is used to detect brain morphological changes in individuals prior to the onset of disease (see, e.g., Toga et al (2006), TINS, 29(3):148-159). For example, in some instances, the typical age of onset for schizophrenia is post-puberty. In some instances, the typical age of onset for schizophrenia is between 20-28 for males and 26-32 for females. For example, in some instances, a typical age of onset for Alzheimer's disease is about 55-80 years. Accordingly, in some embodiments, a PAK inhibitor is administered prophylactically to an individual at risk between about 1 to about 10 years, e.g., 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 years prior to an established and/or typical age range of onset for a CNS disorder.

In prophylactic applications, compounds or compositions containing compounds described herein are administered to an individual, susceptible to or otherwise at risk of a particular disease, disorder or condition. In certain embodiments of this use, the precise amounts of compound administered depend on an individual's state of health, weight, and the like. Furthermore, in some instances, when a compound or composition described herein is administered to an individual, effective amounts for this use depend on the severity and course of the disease, disorder or condition, previous therapy, an individual's health status and response to the drugs, and the judgment of the treating physician.

In certain instances, wherein following administration of a selected dose of a compound or composition described herein, an individual's condition does not improve, upon the doctor's discretion the administration of a compound or composition described herein is optionally administered chronically, that is, for an extended period of time, including throughout the duration of an individual's life in order to ameliorate or otherwise control or limit the symptoms of an individual's disorder, disease or condition.

In certain embodiments, an effective amount of a given agent varies depending upon one or more of a number of factors such as the particular compound, disease or condition and its severity, the identity (e.g., weight) of an individual or host in need of treatment, and is determined according to the particular circumstances surrounding the case, including, e.g., the specific agent being administered, the route of administration, the condition being treated, and an individual or host being treated. In some embodiments, doses administered include those up to the maximum tolerable dose. In certain embodiments, about 0.02 to about 5000 mg per day, from about 1 to about 1500 mg per day, about 1 to about 100 mg/day, about 1 to about 50 mg/day, or about 1 to about 30 mg/day, or about 5 to about 25 mg/day of a compound described herein is administered. In various embodiments, the desired dose is conveniently be presented in a single dose or in divided doses administered simultaneously (or over a short period of time) or at appropriate intervals, for example as two, three, four or more sub-doses per day.

In certain instances, there are a large number of variables in regard to an individual treatment regime, and considerable excursions from these recommended values are considered within the scope described herein. Dosages described herein are optionally altered depending on a number of variables such as, by way of non-limiting example, the activity of the compound used, the disease or condition to be treated, the mode of administration, the requirements of an individual, the severity of the disease or condition being treated, and the judgment of the practitioner.

Toxicity and therapeutic efficacy of such therapeutic regimens are optionally determined by pharmaceutical procedures in cell cultures or experimental animals, including, but not limited to, the determination of the LD₅₀ (the dose lethal to 50% of the population) and the ED₅₀ (the dose therapeutically effective in 50% of the population). The dose ratio between the toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio between LD₅₀ and ED₅₀. Compounds exhibiting high therapeutic indices are preferred. In certain embodiments, data obtained from cell culture assays and animal studies are used in formulating a range of dosage for use in human. In specific embodiments, the dosage of compounds described herein lies within a range of circulating concentrations that include the ED₅₀ with minimal toxicity. The dosage optionally varies within this range depending upon the dosage form employed and the route of administration utilized.

Combination Therapy

In some embodiments, one or more PAK inhibitors are used in combination with one or more other therapeutic agents to treat an individual suffering from a CNS disorder. The combination of PAK inhibitors with a second therapeutic agent (e.g., a typical or atypical antipsychotic agent, an mGluR1 antagonist, an mGluR5 antagonist; an mGluR5 potentiator, a mGluR2 agonist, an alpha7 nicotinic receptor agonist or potentiator, an antioxidant, a neuroprotectant, a trophic factor, an anticholinergic, a beta-secretase inhibitor or the like) allows a reduced dose of both agents to be used thereby reducing the likelihood of side effects associated with higher dose monotherapies. In one embodiment, the dose of a second active agent is reduced in the combination therapy by at least 50% relative to the corresponding monotherapy dose, whereas the PAK inhibitor dose is not reduced relative to the monotherapy dose; in further embodiments, the reduction in dose of a second active agent is at least 75%; in yet a further embodiment, the reduction in dose of a second active agent is at least 90%. In some embodiments, the second therapeutic agent is administered at the same dose as a monotherapy dose, and the addition of a PAK inhibitor to the treatment regimen alleviates symptoms of a CNS disorder that are not treated by monotherapy with the second therapeutic agent. Symptoms and diagnostic criteria for all of the conditions mentioned above are described in detail in the Diagnostic and Statistical Manual of Mental Disorders, fourth edition, American Psychiatric Association (2005) (DSM-IV).

In some embodiments, the combination of a PAK inhibitor and a second therapeutic agent is synergistic (e.g., the effect of the combination is better than the effect of each agent alone). In some embodiments, the combination of a PAK inhibitor and a second therapeutic agent is additive (e.g., the effect of the combination of active agents is about the same as the effect of each agent alone). In some embodiments, an additive effect is due to the PAK inhibitor and the second therapeutic agent modulating the same regulatory pathway. In some embodiments, an additive effect is due to the PAK inhibitor and the second therapeutic agent modulating different regulatory pathways. In some embodiments, an additive effect is due to the PAK inhibitor and the second therapeutic agent treating different symptom groups of the CNS disorder (e.g., a PAK inhibitor treats negative symptoms and the second therapeutic agent treats positive symptoms of schizophrenia). In some embodiments, administration of a second therapeutic agent treats the remainder of the same or different symptoms or groups of symptoms that are not treated by administration of a PAK inhibitor alone.

In some embodiments, administration of a combination of a PAK inhibitor and a second therapeutic agent alleviates side effects that are caused by the second therapeutic agent (e.g., side effects caused by an antipsychotic agent or a nootropic agent). In some embodiments, administration of the second therapeutic agent inhibits metabolism of an administered PAK inhibitor (e.g., the second therapeutic agent blocks a liver enzyme that degrades the PAK inhibitor) thereby increasing efficacy of a PAK inhibitor. In some embodiments, administration of a combination of a PAK inhibitor and a second therapeutic agent (e.g. a second agent that modulates dendritic spine morphology (e.g., minocyline)) improves the therapeutic index of a PAK inhibitor.

Agents for Treating Psychotic Disorders

Where a subject is suffering from or at risk of suffering from a psychotic disorder (e.g., schizophrenia), a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating a psychotic disorder in any combination. Alternatively, a PAK inhibitor composition described herein is administered to a patient who has been prescribed an agent for treating a psychotic disorder. In some embodiments, administration of a PAK inhibitor in combination with an antipsychotic agent has a synergistic effect and provides an improved therapeutic outcome compared to monotherapy with antipsychotic agent or monotherapy with PAK inhibitor. Alternatively, a PAK inhibitor composition described herein is administered to a patient who is non-responsive to, or being unsatisfactorily treated with an antipsychotic agent.

In some embodiments, a PAK inhibitor composition described herein is administered in combination with an antipsychotic having 5-HT2A antagonist activity. In some embodiments, a PAK inhibitor composition described herein is administered in combination with a selective 5-HT2A antagonist.

Examples of therapeutic agents/treatments for treating a psychotic disorder include, but are not limited to, any of the following: typical antipsychotics, e.g., Chlorpromazine (Largactil, Thorazine), Fluphenazine (Prolixin), Haloperidol (Haldol, Serenace), Molindone, Thiothixene (Navane), Thioridazine (Mellaril), Trifluoperazine (Stelazine), Loxapine, Perphenazine, Prochlorperazine (Compazine, Buccastem, Stemetil), Pimozide (Orap), Zuclopenthixol; and atypical antipsychotics, e.g., LY2140023, Clozapine, Risperidone, Olanzapine, Quetiapine, Ziprasidone, Aripiprazole, Paliperidone, Asenapine, Iloperidone, Sertindole, Zotepine, Amisulpride, Bifeprunox, and Melperone.

Agents for Treating Mood Disorders

Where a subject is suffering from or at risk of suffering from a mood disorder (e.g., clinical depression), a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating a mood disorder in any combination. Alternatively, a PAK inhibitor composition described herein is administered to a patient who has been prescribed an agent for treating a mood disorder. Alternatively, a PAK inhibitor composition described herein is administered to a patient who is non-responsive to or being unsatisfactorily treated with an agent for treating a mood disorder.

Examples of therapeutic agents/treatments for treating a mood disorder include, but are not limited to, any of the following: selective serotonin reuptake inhibitors (SSRIs) such as citalopram (Celexa), escitalopram (Lexapro, Esipram), fluoxetine (Prozac), paroxetine (Paxil, Seroxat), sertraline (Zoloft), fluvoxamine (Luvox); serotonin-norepinephrine reuptake inhibitors (SNRIs) such as venlafaxine (Effexor), desvenlafaxine, nefazodone, milnacipran, duloxetine (Cymbalta), bicifadine; tricyclic antidepressants such as amitriptyline, amoxapine, butriptyline, clomipramine, desipramine, dosulepin, doxepin, impramine, lofepramine, nortriptyline; monoamine oxidase inhibitors (MAOIs) such as isocarboxazid, linezolid, moclobemide, nialamide, phenelzine, selegiline, tranylcypromine, trimipramine; and other agents such as mirtazapine, reboxetine, viloxazine, malprotiline, and bupropion.

Agents for Treating Epilepsy

Where a subject is suffering from or at risk of suffering from epilepsy, a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating epilepsy in any combination. Alternatively, a PAK inhibitor composition described herein is administered to a patient who has been prescribed an agent for treating epilepsy. Alternatively, a PAK inhibitor composition described herein is administered to a patient who is refractory to or being unsatisfactorily treated with an agent for treating epilepsy.

Examples of therapeutic agents/treatments for treating epilepsy include, but are not limited to, any of the following: carbamazepine, clobazam, clonazepam, ethosuximide, felbamate, fosphenyloin, gabapentin, lamotrigine, levetiracetam, oxcarbazepine, phenobarbital, phenyloin, pregabalin, primidone, sodium valproate, tiagabine, topiramate, valproate semisodium, valproic acid, vigabatrin, and zonisamide.

Agents for Treating Huntington's Disease

Where a subject is suffering from or at risk of suffering from Huntington's disease, a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating Huntington's disease in any combination. Alternatively, a PAK inhibitor composition described herein is administered to a patient who has been prescribed an agent for treating Huntington's disease. Alternatively, a PAK inhibitor composition described herein is administered to a patient who is refractory to or being unsatisfactorily treated with an agent for treating Huntington's disease.

Examples of therapeutic agents/treatments for treating Huntington's disease include, but are not limited to, any of the following: omega-3 fatty acids, miraxion, Haloperidol, dopamine receptor blockers, creatine, cystamine, cysteamine, clonazepam, clozapine, Coenzyme Q10, minocycline, antioxidants, antidepressants (notably, but not exclusively, selective serotonin reuptake inhibitors SSRIs, such as sertraline, fluoxetine, and paroxetine), select dopamine antagonists, such as tetrabenazine; and RNAi knockdown of mutant huntingtin (mHtt).

Agents for Treating Parkinson's Disease

Where a subject is suffering from or at risk of suffering from Parkinson's Disease, a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating Parkinson's disease in any combination. Alternatively, a PAK inhibitor composition described herein is administered to a patient who has been prescribed an agent for treating Parkinson's disease. Alternatively, a PAK inhibitor composition described herein is administered to a patient who is refractory to or being unsatisfactorily treated with an agent for treating Parkinson's disease.

Examples of therapeutic agents/treatments for treating Parkinson's Disease include, but are not limited to any of the following: L-dopa, carbidopa, benserazide, tolcapone, entacapone, bromocriptine, pergolide, pramipexole, ropinirole, cabergoline, apomorphine, lisuride, selegiline, or rasagiline.

Group I mGluR Antagonists

In some embodiments, one or more PAK inhibitors are used in combination with one or more Group I metabotropic glutamate receptor (mGluR) antagonists (e.g., mGluR5 antagonists) to treat an individual suffering from a CNS disorder. The combination of PAK inhibitors with Group I mGluR antagonists allows a reduced dose of both agents to be used thereby reducing the likelihood of side effects associated with higher dose monotherapies.

In some embodiments, reduction of signaling from a Group I mGluR (mGluR5) in vivo by genetic engineering (using mGluR5 knock-out heterozygote animals) leads to a reversal of the dendritic spine and behavioral defects. In some instances, where an individual is suffering from or at risk of suffering from a CNS disorder, a PAK inhibitor composition described herein is optionally used together with one or Group I mGluR antagonists. Group I mGluR antagonists include antagonists that are mGluR1-selective antagonists, mGluR5-selective antagonists, or antagonists that antagonize both mGluR1 and mGluR5. In some embodiments, a PAK inhibitor composition is used in combination with an mGluR5-selective antagonist. In some embodiments, a PAK inhibitor composition is used in combination with an mGluR1-selective antagonist. In some embodiments, a PAK inhibitor composition is used in combination with a Group I mGluR antagonist that antagonizes both mGluR1 and mGluR5 (i.e., an antagonist that is not selective for mGluR1 or mGluR5). As used herein, the term “selective antagonist” indicates that the antagonist has an ED₅₀ for antagonizing a first receptor (e.g., mGluR5) that is at least about 10 fold to about 1000 fold lower, e.g., 11, 20, 30, 40, 50, 100, 105, 125, 135, 150, 200, 300, 400, 500, 600, 700, 800, 900, or any other fold lower from about 10 fold to about 1000 fold lower than the ED₅₀ for antagonism of a second receptor (e.g., mGluR1).

Examples of Group I mGluR antagonists include, but are not limited to, any of the following (E)-6-methyl-2-styryl-pyridine (SIB 1893), 6-methyl-2-(phenylazo)-3-pyridinol, .alpha.-methyl-4-carboxyphenylglycine (MCPG), or 2-methyl-6-(phenylethynyl)-pyridine (MPEP). Examples of Group I mGluR antagonists also include those described in, e.g., U.S. patent application Ser. Nos. 10/076,618; 10/211,523; and 10/766,948. Examples of mGluR5-selective antagonists include, but are not limited to those described in, e.g., U.S. Pat. No. 7,205,411 and U.S. patent application Ser. No. 11/523,873. Examples of mGluR1-selective antagonists include, but are not limited to, those described in, e.g., U.S. Pat. No. 6,482,824.

In some embodiments, the mGluR Group I antagonist is AIDA (1-aminoindan-1,5-dicarboxylic acid); ACDPP (3-Amino-6-chloro-5-dimethylamino-N-2-pyridinylpyrazinecarboxamide hydrochloride; DL-AP3 (DL-2-Amino-3-phosphonopropionic acid); BAY-36-7620 ((3aS,6aS)-Hexahydro-5-methylene-6a-(2-naphthalenylmethyl)-1H-cyclopenta[c]furan-1-one); Fenobam; 4 CPG ((S)4-carboxyphenylglycine); (S)-4C3HPG ((S)-4-carboxy-3-hydroxyphenylglycine); CPCCOEt (7-hydroxyiminocyclopropan[b]chromen-1a-carboxylic acid ethyl ester); LY 367385 ((S)-(+)-a-Amino-4-carboxy-2-methylbenzeneacetic acid); LY 456236 hydrochloride (6-methoxy-N-(4-methoxyphenyl)quinazolin-4-amine, MPMQ hydrochloride); 3-MATIDA (a-Amino-5-carboxy-3-methyl-2-thiopheneacetic acid); MCPG (α-methyl-4-carboxyphenylglycine); MPEP (2-methyl-6-(phenylethynyl)-pyridine); (MTEP) 3-[(2-methyl-1,3-thiazol-4-yl)ethynyl]-pyridine; PHCCC (N-Phenyl-7-(hydroxyimino)cyclopropa[b]chromen-1a-carboxamide; SIB 1757 (6-Methyl-2-(phenylazo)-3-pyridinol; SIB 1893 (2-Methyl-6-(2-phenylethenyl)pyridine; YM 298198 hydrochloride (6-Amino-N-cyclohexyl-N,3-dimethylthiazolo[3,2-a]benzimidazole-2-carboxamidehydrochloride); (YM-193167 (6-amino-N-cyclohexyl-N,3-dimethylthiazolo[3,2-a]benzimidazole-2-carboxamide); (NPS 2390 (Quinoxaline-2-carboxylic acid adamantan-1-ylamide); 3-(5-(pyridin-2-yl)-2H-tetrazol-2-yl)benzonitrile; 3-[3-fluoro-5-(5-pyridin-2-yl-2H-tetrazol-2-yl)phenyl]-4-methylpyridine; 3-fluoro-5-(5-pyridin-2-yl-2H-tetrazol-2-yl)benzonitrile; N-cyclohexyl-6-{[(2-methoxyethyl)(methyl)amino]methyl}-N-methylthiazolo[3,2-a]benzimidazole-2-carboxamide (YM-202074); Desmethyl-YM298198 (6-Amino-N-cyclohexyl-3-methylthiazolo[3,2-a]benzimidazole-2-carboxamide hydrochloride); MPEP hydrochloride (2-Methyl-6-(phenylethynyl)pyridine hydrochloride); (S)-MCPG ((S)-a-Methyl-4-carboxyphenylglycine); (RS)-MCPG ((RS)-a-Methyl-4-carboxyphenylglycine); E4CPG ((RS)-a-Ethyl-4-carboxyphenylglycine); Hexylhomoibotenic acid (a-Amino-4-hexyl-2,3-dihydro-3-oxo-5-isoxazolepropanoic acid; HexylHIBO); (S)-Hexylhomoibotenic acid ((S)-a-Amino-4-hexyl-2,3-dihydro-3-oxo-5-isoxazolepropanoic acid; (S)-HexylHIBO); EMQMCM (3-ethyl-2-methyl-quinolin-6-yl)-(4-methoxy-cyclohexyl)-methanone methanesulfonate); JNJ 16259685; R214127 (1-(3,4-dihydro-2H-pyrano[2,3-b]quinolin-7-yl)-2-phenyl-1-ethanone); (S)-3-Carboxy-4-hydroxyphenylglycine ((S)-3C4HPG); Anti-mGlu5 blocking peptide ([K]-SSPKYDTLIIRDYTQSSSSL); DFB (3,3′-Difluorobenzaldazine); DMeOB ([(3-Methoxyphenyl)methylene]hydrazone-3-methoxybenzaldehyde); Anti-mGlu₅ (([K]-SSPKYDTLIIRDYTQSSSSL); reluzole; or combinations thereof.

In some embodiments, the modulator of a Group I mGluR is S-(4-Fluoro-phenyl)-{3-[3-(4-fluoro-phenyl)-[1,2,4]oxadiazol-5-yl]-piperidin-1-yl}-methanone (ADX47273) (Positive allosteric modulator); 4-[1-(2-fluoropyridin-3-yl)-5-methyl-1H-1,2,3-triazol-4-yl]-N-isopropyl-N-methyl-3,6-dihydropyridine-1(2H)-carboxamide (FTIDC); 6-(3-methoxy-4-(pyridin-2-yl)phenyl)imidazole[2,1-b]thiazole; 2-(2-methoxy-4-(4-(pyridin-2-yl)oxazol-2-yl)phenyl)acetonitrile; 2-(4-(benzo[d]oxazol-2-yl)-2-methoxyphenyl)acetonitrile; 2-(4-(2,3-dihydro-1H-inden-2-ylamino)4a,5,6,7,8,8a-hexahydroquinazolin-2ylthio)ethanol; or combinations thereof.

In some embodiments, where a Group I mGluR antagonist (e.g., an mGluR5 antagonist) is administered in combination with a PAK inhibitor, the dose of the Group I mGluR antagonist ranges from about 0.001 mg/kg/day to about 30.0 mg/kg/day, e.g., about 0.005 mg/kg/day, 0.009 mg/kg/day, 0.010 mg/kg/day, 0.050 mg/kg/day, 0.20 mg/kg/day, 0.50 mg/kg/day, 0.75 mg/kg/day, 1.0 mg/kg/day, 2.0 mg/kg/day, 3.5 mg/kg/day, 4.5 mg/kg/day, 5.0 mg/kg/day, 6.2 mg/kg/day, 6.8 mg/kg/day, 7.0 mg/kg/day, 10.0 mg/kg/day, 15 mg/kg/day, 20 mg/kg/day, 25 mg/kg/day, or any other dose from about 0.001 mg/kg/day to about 10.0 mg/kg/day, from about 0.001 mg/kg/day to about 20.0 mg/kg/day, or from about 0.01 mg/kg/day to about 20.0 mg/kg/day.

In some embodiments, the combination treatment comprises administering a combined dosage form that is a pharmacological composition comprising a therapeutically effective amount of a PAK inhibitor and a Group I mGluR antagonist (e.g., an mGluR5-selective antagonist) as described herein. In some embodiments, the pharmacological composition comprises a PAK inhibitor compound and an mGluR5-selective antagonist selected from U.S. Pat. No. 7,205,411.

mGluR Agonists

In some embodiments, a second therapeutic agent used in combination with a PAK inhibitor is a Group I mGluR1 agonist. Examples of mGluR1 agonists and/or mGluR1 potentiators include and are not limited to ACPT-I ((1S,3R,4S)-1-aminocyclopentane-1,3,4-tricarboxylic acid); L-AP4 (L-(+)-2-Amino-4-phosphonobutyric acid); (S)-3,4-DCPG ((S)-3,4-dicarboxyphenylglycine); (RS)-3,4-DCPG ((RS)-3,4-dicarboxyphenylglycine); (RS)-4-phosphonophenylglycine ((RS)PPG); AMN082 (,N′-bis(diphenylmethyl)-1,2-ethanediamine dihydrochloride); DCG-IV ((2S,2′R,3′R)-2-(2′,3′-dicarboxycyclopropyl)glycine) or the like. In some embodiments, an mGluR1 agonist is AMN082. In some embodiments, a second therapeutic agent is a mGluR2/3 agonist or mGluR2/3 potentiator. Examples of mGluR2/3 agonists include and are not limited to LY389795 ((−)-2-thia-4-aminobicyclo-hexane-4,6-dicarboxylate); LY379268 ((−)-2-oxa-4-aminobicyclo-hexane-4,6-dicarboxylate); LY354740 ((+)-2-aminobicyclo-hexane-2,6dicarboxylate); DCG-IV ((2S,2′R,3′R)-2-(2′,3′-d icarboxycyclopropyl)glycine); 2R,4R-APDC (2R,4R-4-aminopyrrolidine-2,4-dicarboxylate), (S)-3C4HPG ((S)-3-carboxy-4-hydroxyphenylglycine); (S)-4C3HPG ((S)-4-carboxy-3-hydroxyphenylglycine); L-CCG-I ((2S,1′S,2′S)-2-(carboxycyclopropyl)glycine); and/or combinations thereof. Examples of mGluR2 agonists or mGluR2 potentiators include and are not limited to positive allosteric modulators of mGluR2, including ADX71149 (Addex Partner). Examples of mGluR5 agonists or mGluR5 potentiators include and are not limited to MPEP, (RS)-2-chloro-5-hydroxyphenylglycine (CHPG), 1S,3R-1-amino-1,3-cyclopentanedicarboxylate (ACPD) or the like.

Apha7 Nicotinic Receptor Modulators

In some embodiments, one or more PAK inhibitors are used in combination with one or more alpha7 nicotinic receptor modulators to treat an individual suffering from a CNS disorder. Alpha7 nicotinic receptor modulators include alpha7 nicotinic receptor agonists, alpha7 nicotinic receptor antagonists, and/or alpha7 nicotinic receptor modulators positive allosteric potentiators. The combination of PAK inhibitors with alpha7 nicotinic receptor modulators allows a reduced dose of both agents to be used thereby reducing the likelihood of side effects associated with higher dose monotherapies.

Examples of alpha7 nicotinic receptor agonists include and are not limited to (+)-N-(1-azabicyclo[2.2.2]oct-3-yl)benzo[b]furan-2-carboxamide, PHA-709829, PNU-282,987, A-582941, TC-1698, TC-5619, GTS-21, SSR180711, tropisetron or the like. Examples of alpha7 nicotinic receptor antagonists include α-conotoxin, quinolizidine or the like. Alpha7 nicotinic receptor allosteric potentiators include PNU-120596, NS-1738, XY4083, A-867744, EVP-6124 (Envivo), or the like.

Cholinesterase Inhibitors

Where a subject is suffering from or at risk of suffering from Alzheimer's disease, a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating Alzheimer's disease in any combination. In some embodiments, a PAK inhibitor composition described herein is administered to a patient who has been prescribed an acetylcholinesterase inhibitor. In some embodiments, administration of a PAK inhibitor in combination with an acetylcholinesterase inhibitor has a synergistic effect and provides an improved therapeutic outcome compared to monotherapy with acetylcholinesterase inhibitors or monotherapy with PAK inhibitor. Alternatively, a PAK inhibitor composition described herein is administered to an individual who is non-responsive to, or being unsatisfactorily treated with an acetylcholinesterase inhibitor. Example of acetylcholinesterase inhibitors include donepezil (Aricept), galantamine (Razadyne), rivastigmine (Exelon and Exelon Patch).

Muscarinic Modulators

In some embodiments, a PAK inhibitor composition described herein is administered to a patient in combination with a muscarinic receptor modulator. In some embodiments, the muscarinic receptor modulator is a M1 muscarinic receptor agonist. In some embodiments, the muscarinic receptor modulator is AF102B, AF150(S), AF267B, N-{1-[3-(3-oxo-2,3-dihydrobenzo[1,4]oxazin-4-yl)Propyl]piperidin-4-yl}-2-phenylacetamide, BRL-55473, NXS-292, NXS-267, MCD-386, AZD-6088, N-Desmethylclozapineor a similar compound. In some embodiments, the muscarinic receptor modulator is a positive allosteric modulator of M1 muscarinic receptors. Examples of positive allosteric M1 muscarinic receptor modulators include, but are not limited to, VU0119498, VU0027414, VU0090157, VU0029767, BQCA, TBPB or 77-LH-28-1. In some embodiments, the muscarinic receptor modulator is a M4 muscarinic receptor agonist. In some embodiments, the muscarinic receptor modulator is a positive allosteric modulator of M4 muscarinic receptors. Examples for positive allosteric M4 muscarinic receptor modulators include, but are not limited to, VU0010010, VU0152099, VU0152100, or LY2033298.

NMDA Receptor Antagonists

Where a subject is suffering from or at risk of suffering from Alzheimer's disease, a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating Alzheimer's disease in any combination. In some embodiments, a PAK inhibitor composition described herein is administered to a patient who has been prescribed an NMDA receptor antagonist. Examples of NMDA receptor antagonists useful in the methods and compositions described herein include and are not limited to memantine.

Neuroprotectants

In some embodiments, a PAK inhibitor or a composition thereof described herein is administered in combination with a neuroprotectant such as, for example, minocycline, resveratrol or the like.

Trophic Factors

In some embodiments, a PAK inhibitor or a composition thereof described herein is administered in combination with a trophic agent including, by way of example, glial derived nerve factor (GDNF), brain derived nerve factor (BDNF) or the like.

Antioxidants

Where a subject is suffering from or at risk of suffering from a CNS disorder (e.g., Alzheimer's disease, Mild Cognitive Impairment), a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating the CNS disorder in any combination. In some embodiments, a PAK inhibitor composition described herein is administered to a patient who is taking or has been prescribed an antioxidant. Examples of antioxidants useful in the methods and compositions described herein include and are not limited to ubiquinone, aged garlic extract, curcumin, lipoic acid, beta-carotene, melatonin, resveratrol, Ginkgo biloba extract, vitamin C, vitamin E or the like.

Metal Protein Attenuating Compounds

Where a subject is suffering from or at risk of suffering from a CNS disorder (e.g., Alzheimer's disease, Parkinson's disease), a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating the CNS disorder in any combination. In some embodiments, a PAK inhibitor composition described herein is administered to a patient who has been prescribed a Metal Protein Attenuating agent. Examples of Metal Protein Attenuating agents useful in the methods and compositions described herein include and are not limited to 8-Hydroxyquinoline, iodochlorhydroxyquin or the like and derivatives thereof.

Beta-Secretase Inhibitors

Where a subject is suffering from or at risk of suffering from a CNS disorder (e.g., Alzheimer's disease), a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating the CNS disorder in any combination. In some embodiments, a PAK inhibitor composition described herein is administered to a patient who has been prescribed a beta secretase inhibitor. Examples of beta secretase inhibitors useful in the methods and compositions described herein include and are not limited to LY450139, 2-Aminoquinazolines compounds described in J. Med. Chem. 50 (18): 4261-4264, beta secretase inhibitors described therein are incorporated herein by reference, or the like.

Gamma Secretase Inhibitors

Where a subject is suffering from or at risk of suffering from a CNS disorder (e.g., Alzheimer's disease), a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating the CNS disorder in any combination. In some embodiments, a PAK inhibitor composition described herein is administered to a patient who has been prescribed a beta secretase inhibitor. Examples of beta secretase inhibitors useful in the methods and compositions described herein include and are not limited to LY-411575, (2S)-2-hydroxy-3-methyl-N-((1S)-1-methyl-2-{[(1S)-3-methyl-2-oxo-2,3,4,5-tetrahydro-1H-3-benzazepin-1-yl]amino}-2-oxoethyl)butanamide (semagacestat), (R)-2-(3-Fluoro-4-phenylphenyl)propanoic acid (Tarenflurbil), or the like.

Antibodies

Where a subject is suffering from or at risk of suffering from a CNS disorder (e.g., Alzheimer's disease), a PAK inhibitor composition described herein is optionally used together with one or more agents or methods for treating the CNS disorder in any combination. In some embodiments, a PAK inhibitor composition described herein is administered to a patient who has been prescribed an Abeta antibody. Examples of antibodies useful in the methods and compositions described herein include and are not limited an Abeta antibody (e.g., bapineuzumab), PAK antibodies (e.g., ABIN237914) or the like.

Other Agents

In some embodiments, one or more PAK inhibitors are used in combination with one or more agents that modulate dendritic spine morphology or synaptic function. Examples of agents that modulate dendritic spine morphology include minocycline, trophic factors (e.g., brain derived neutrophic factor, glial cell-derived neurtrophic factor), or anesthetics that modulate spine motility, or the like. In some embodiments, one or more PAK inhibitors are used in combination with one or more agents that modulate cognition. In some embodiments, a second therapeutic agent is a nootropic agent that enhances cognition. Examples of nootropic agents include and are not limited to piracetam, pramiracetam, oxiracetam, and aniracetam.

Blood Brain Barrier Facilitators

In some instances, a PAK inhibitor is optionally administered in combination with a blood brain barrier facilitator. In certain embodiments, an agent that facilitates the transport of a PAK inhibitor is covalently attached to the PAK inhibitor. In some instances, PAK inhibitors described herein are modified by covalent attachment to a lipophilic carrier or co-formulation with a lipophilic carrier. In some embodiments, a PAK inhibitor is covalently attached to a lipophilic carrier, such as e.g., DHA, or a fatty acid. In some embodiments, a PAK inhibitor is covalently attached to artificial low density lipoprotein particles. In some instances, carrier systems facilitate the passage of PAK inhibitors described herein across the blood-brain barrier and include but are not limited to, the use of a dihydropyridine pyridinium salt carrier redox system for delivery of drug species across the blood brain barrier. In some instances a PAK inhibitor described herein is coupled to a lipophilic phosphonate derivative. In certain instances, PAK inhibitors described herein are conjugated to PEG-oligomers/polymers or aprotinin derivatives and analogs. In some instances, an increase in influx of a PAK inhibitor described herein across the blood brain barrier is achieved by modifying A PAK inhibitor described herein (e.g., by reducing or increasing the number of charged groups on the compound) and enhancing affinity for a blood brain barrier transporter. In certain instances, a PAK inhibitor is co-administered with an an agent that reduces or inhibits efflux across the blood brain barrier, e.g. an inhibitor of P-glycoprotein pump (PGP) mediated efflux (e.g., cyclosporin, SCH66336 (lonafarnib, Schering)).

In some embodiments, compounds of Formula I-XV are optionally administered in combination with, e.g., compounds described in U.S. Pat. Nos. 5,863,532, 6,191,169, 6,248,549, and 6,498,163; U.S. Patent Applications 200200045564, 20020086390, 20020106690, 20020142325, 20030124107, 20030166623, 20040091992, 20040102623, 20040208880, 200500203114, 20050037965, 20050080002, and 20050233965, 20060088897; EP Patent Publication 1492871; PCT patent publication WO 9902701; PCT patent publication WO 2008/047307; Kumar et al., (2006), Nat. Rev. Cancer, 6:459; and Eswaran et al., (2007), Structure, 15:201-213, all of which are incorporated herein by reference for disclosure of kinase inhibitors and/or PAK inhibitors described therein.

In some embodiments, compounds of Formula I-XV are optionally administered in combination with compounds including and not limited to BMS-387032; SNS-032; CHI4-258; TKI-258; EKB-569; JNJ-7706621; PKC-412; staurosporine; SU-14813; sunitinib; N-(3-chloro-4-fluoro-phenyl)-7-methoxy-6-(3-morpholin-4-ylpropoxy)quinazolin-4-amine (gefitinib), VX-680; MK-0457; combinations thereof; or salts, prodrugs thereof.

In some embodiments, compounds of Formula I-XV are optionally administered in combination with a polypeptide comprising an amino acid sequence about 80% to about 100% identical, e.g., 85%, 90%, 92%, 93%, 95%, 96%, 97%, 98%, 99%, or any other percent from about 80% to about 100% identical the following amino acid sequence:

HTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLEFYN SKKTSNSQ KYMSFTDKS

The above sequence corresponds to the PAK autoinhibitory domain (PAD) polypeptide amino acids 83-149 of PAK1 polypeptide as described in, e.g., Zhao et al (1998). In some embodiments, the PAK inhibitor is a fusion protein comprising the above-described PAD amino acid sequence. In some embodiments, in order to facilitate cell penetration the fusion polypeptide (e.g., N-terminal or C-terminal) further comprises a polybasic protein transduction domain (PTD) amino acid sequence, e.g.: RKKRRQRR; YARAAARQARA; THRLPRRRRRR; or GGRRARRRRRR.

In some embodiments, in order to enhance uptake into the brain, the fusion polypeptide further comprises a human insulin receptor antibody as described in U.S. patent application Ser. No. 11/245,546.

In some embodiments, compounds of Formula I-XV are optionally administered in combination with a peptide inhibitor comprising a sequence at least 60% to 100%, e.g., 65%, 70%, 75%, 80%, 85%, 90%, 92%, 93%, 95%, 96%, 97%, 98%, 99%, or any other percent from about 60% to about 100% identical the following amino acid sequence: PPVIAPREHTKSVYTRS as described in, e.g., Zhao et al (2006), Nat Neurosci, 9(2):234-242. In some embodiments, the peptide sequence further comprises a PTD amino acid sequence as described above.

In some embodiments, compounds of Formula I-XV are optionally administered in combination with a polypeptide comprising an amino acid sequence at least 80% to 100%, e.g., 85%, 90%, 92%, 93%, 95%, 96%, 97%, 98%, 99%, or any other percent from about 80% to about 100% identical to the FMRP1 protein (GenBank Accession No. Q06787), where the polypeptide is able to bind with a PAK (for example, PAK1, PAK2, PAK3, PAK4, PAK5 and/or PAK6). In some embodiments compounds of Formula I-XV are optionally administered in combination with a polypeptide comprising an amino acid sequence at least 80% to 100%, e.g., 85%, 90%, 92%, 93%, 95%, 96%, 97%, 98%, 99%, or any other percent from about 80% to about 100% identical to the FMRPI protein (GenBank Accession No. Q06787), where the polypeptide is able to bind with a Group I PAK, such as, for example PAK1 (see, e.g., Hayashi et al (2007), Proc Natl Acad Sci USA, 104(27):11489-11494. In some embodiments, compounds of Formula I-XV are optionally administered in combination with a polypeptide comprising a fragment of human FMRP1 protein with an amino acid sequence at least 80% to 100%, e.g., 85%, 90%, 92%, 93%, 95%, 96%, 97%, 98%, 99%, or any other percent from about 80% to about 100% identical to the sequence of amino acids 207-425 of the human FMRP1 protein (i.e., comprising the KH1 and KH2 domains), where the polypeptide is able to bind to PAK1.

In some embodiments, compounds of Formula I-XV are optionally administered in combination with a polypeptide comprising an amino acid sequence at least 80% to 100%, e.g., 85%, 90%, 92%, 93%, 95%, 96%, 97%, 98%, 99%, or any other percent from about 80% to about 100% identical to at least five, at least ten at least twenty, at least thirty, at least forty, at least fifty, at least sixty, at least seventy, at least eighty, at least ninety contiguous amino acids of the huntingtin (htt) protein (GenBank Accession No. NP 002102, gi 90903231), where the polypeptide is able to bind to a Group 1 PAK (for example, PAK1, PAK2, and/or PAK3). In some embodiments, compounds of Formula I-XV are optionally administered in combination with a polypeptide comprising an amino acid sequence at least 80% to 100%, e.g., 85%, 90%, 92%, 93%, 95%, 96%, 97%, 98%, 99%, or any other percent from about 80% to about 100% identical to at least a portion of the huntingtin (htt) protein (GenBank Accession No. NP 002102, gi 90903231), where the polypeptide is able to bind to PAK1. In some embodiments, compounds of Formula I-XV are optionally administered in combination with a polypeptide comprising a fragment of human huntingtin protein with an amino acid sequence at least 80% to 100%, e.g., 85%, 90%, 92%, 93%, 95%, 96%, 97%, 98%, 99%, or any other percent from about 80% to about 100% identical to a sequence of at least five, at least ten, at least twenty, at least thirty, at least forty, at least fifty, at least sixty, at least seventy, at least eighty, at least ninety, or at least 100 contiguous amino acids of the human huntingtin protein that is outside of the sequence encoded by exon 1 of the htt gene (i.e., a fragment that does not contain poly glutamate domains), where the polypeptide binds a PAK. In some embodiments, compounds of Formula I-XV are optionally administered in combination with a polypeptide comprising a fragment of human huntingtin protein with an amino acid sequence at least 80% identical to a sequence of the human huntingtin protein that is outside of the sequence encoded by exon 1 of the htt gene (i.e., a fragment that does not contain poly glutamate domains), where the polypeptide binds PAK1.

Upstream Regulators of p21 Activated Kinases

In certain embodiments, compounds of Formula I-XV are optionally administered in, combination with an indirect PAK modulator (e.g., an indirect PAK inhibitor) that affects the activity of a molecule that acts in a signaling pathway upstream of PAK (upstream regulators of PAK). Upstream effectors of PAK include, but are not limited to: TrkB receptors; NMDA receptors; EphB receptors; adenosine receptors; estrogen receptors; integrins; FMRP; Rho-family GTPases, including Cdc42, Rac (including but not limited to Rac1 and Rac2), CDK5, PI3 kinases, NCK, PDK1, EKT, GRB2, Chp, TC10, Tcl, and Wrch-1; guanine nucleotide exchange factors (“GEFs”), such as but not limited to GEFT, members of the Dbl family of GEFs, p21-activated kinase interacting exchange factor (PIX), DEF6, Zizimin 1, Vav1, Vav2, Dbs, members of the DOCK180 family, Kalirin-7, and Tiam1; G protein-coupled receptor kinase-interacting protein 1 (GITI), CIB1, filamin A, Etk/Bmx, and sphingosine.

Modulators of NMDA receptor include, but are not limited to, 1-aminoadamantane, dextromethorphan, dextrorphan, ibogaine, ketamine, nitrous oxide, phencyclidine, riluzole, tiletamine, memantine, neramexane, dizocilpine, aptiganel, remacimide, 7-chlorokynurenate, DCKA (5,7-dichlorokynurenic acid), kynurenic acid, 1-aminocyclopropanecarboxylic acid (ACPC), AP7 (2-amino-7-phosphonoheptanoic acid), APV (R-2-amino-5-phosphonopentanoate), CPPene (3-[(R)-2-carboxypiperazin-4-yl]-prop-2-enyl-1-phosphonic acid); (+)-(1S,2S)-1-(4-hydroxy-phenyl)-2-(4-hydroxy-4-phenylpiperidino)-1-pro-panol; (1S,2S)-1-(4-hydroxy-3-methoxyphenyl)-2-(4-hydroxy-4-phenylpiperi-dino)-1-propanol; (3R,4S)-3-(4-(4-fluorophenyl)-4-hydroxypiperidin-1-yl-)-chroman-4,7-diol; (1R*,2R*)-1-(4-hydroxy-3-methylphenyl)-2-(4-(4-fluoro-phenyl)-4-hydroxypiperidin-1-yl)-propan-1-ol-mesylate; and/or combinations thereof.

Modulators of estrogen receptors include, and are not limited to, PPT (4,4′,4″-(4-Propyl-[1H]-pyrazole-1,3,5-triyl)trisphenol); SKF-82958 (6-chloro-7,8-dihydroxy-3-allyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine); estrogen; estradiol; estradiol derivatives, including but not limited to 17-β estradiol, estrone, estriol, ERβ-131, phytoestrogen, MK 101 (bioNovo); VG-1010 (bioNovo); DPN (diarylpropiolitrile); ERB-041; WAY-202196; WAY-214156; genistein; estrogen; estradiol; estradiol derivatives, including but not limited to 17-β estradiol, estrone, estriol, benzopyrans and triazolo-tetrahydrofluorenones, disclosed in U.S. Pat. No. 7,279,499, and Parker et al., Bioorg. & Med. Chem. Ltrs. 16: 4652-4656 (2006), each of which is incorporated herein by reference for such disclosure.

Modulators of TrkB include by way of example, neutorophic factors including BDNF and GDNF. Modulators of EphB include XL647 (Exelixis), EphB modulator compounds described in WO/2006081418 and US Appl. Pub. No. 20080300245, incorporated herein by reference for such disclosure, or the like.

Modulators of integrins include by way of example, ATN-161, PF-04605412, MEDI-522, Volociximab, natalizumab, Volociximab, Ro 27-2771, Ro 27-2441, etaracizumab, CNTO-95, JSM6427, cilengitide, R411 (Roche), EMD 121974, integrin antagonist compounds described in J. Med. Chem., 2002, 45 (16), pp 3451-3457, incorporated herein by reference for such disclosure, or the like.

Adenosine receptor modulators include, by way of example, theophylline, 8-Cyclopentyl-1,3-dimethylxanthine (CPX), 8-Cyclopentyl-1,3-dipropylxanthine (DPCPX), 8-Phenyl-1,3-dipropylxanthine, PSB 36, istradefylline, SCH-58261, SCH-442,416, ZM-241,385, CVT-6883, MRS-1706, MRS-1754, PSB-603, PSB-0788, PSB-1115, MRS-1191, MRS-1220, MRS-1334, MRS-1523, MRS-3777, MRE3008F20, PSB-10, PSB-11, VUF-5574, N6-Cyclopentyladenosine, CCPA, T-MeCCPA, GR 79236, SDZ WAG 99, ATL-146e, CGS-21680, Regadenoson, 5′-N-ethylcarboxamidoadenosine, BAY 60-6583, LUF-5835, LUF-5845, Hexynyl)-N-methyladenosine, CF-101 (IB-MECA), 2-Cl-IB-MECA, CP-532,903, MRS-3558, Rosuvastatin, KW-3902, SLV320, mefloquine, regadenoson, or the like.

In some embodiments, compounds reducing PAK levels decrease PAK transcription or translation or reduce RNA or protein levels. In some embodiments, a compound that decreases PAK levels is an upstream effector of PAK. In some embodiments, exogenous expression of the activated forms of the Rho family GTPases Chp and cdc42 in cells leads to increased activation of PAK while at the same time increasing turnover of the PAK protein, significantly lowering its level in the cell (Hubsman et al. (2007) Biochem. J. 404: 487-497). PAK clearance agents include agents that increase expression of one or more Rho family GTPases and/or one or more guanine nucleotide exchange factors (GEFs) that regulate the activity of Rho family GTPases, in which overexpression of a Rho family GTPase and/or a GEF results in lower levels of PAK protein in cells. PAK clearance agents also include agonists of Rho family GTPases, as well as agonists of GTP exchange factors that activate Rho family GTPases, such as but not limited to agonists of GEFs of the Dbl family that activate Rho family GTPases.

Overexpression of a Rho family GTPase is optionally by means of introducing a nucleic acid expression construct into the cells or by administering a compound that induces transcription of the endogenous gene encoding the GTPase. In some embodiments, the Rho family GTPase is Rac (e.g., Rac1, Rac2, or Rac3), cdc42, Chp, TC10, Tcl, or Wrnch-1. For example, a Rho family GTPase includes Rac1, Rac2, Rac3, or cdc42. A gene introduced into cells that encodes a Rho family GTPase optionally encodes a mutant form of the gene, for example, a more active form (for example, a constitutively active form, Hubsman et al. (2007) Biochem. J. 404: 487-497). In some embodiments, a PAK clearance agent is, for example, a nucleic acid encoding a Rho family GTPase, in which the Rho family GTPase is expressed from a constitutive or inducible promoter. PAK levels in some embodiments are reduced by a compound that directly or indirectly enhances expression of an endogenous gene encoding a Rho family GTPase.

In some embodiments, compounds of Formula I-XV are optionally administered in combination with a PAK clearance agent.

In some embodiments, compounds of Formula I-XV are optionally administered in combination with a compound that directly or indirectly decreases the activation or activity of the upstream effectors of PAK. For example, in some embodiments a compound that inhibits the GTPase activity of the small Rho-family GTPases such as Rac and cdc42 thereby reduce the activation of PAK kinase. In some embodiments, the compound that decreases PAK activation is by secramine that inhibits cdc42 activation, binding to membranes and GTP in the cell (Pelish et al. (2005) Nat. Chem. Biol. 2: 39-46). In some embodiments, PAK activation is decreased by EHT 1864, a small molecule that inhibits Rac1, Rac1b, Rac2 and Rac3 function by preventing binding to guanine nucleotide association and engagement with downstream effectors (Shutes et al. (2007) J. Biol. Chem. 49: 35666-35678). In some embodiments, PAK activation is also decreased by the NSC23766 small molecule that binds directly to Rac1 and prevents its activation by Rac-specific RhoGEFs (Gao et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101: 7618-7623). In some embodiments, PAK activation is also decreased by the 16 kDa fragment of prolactin (16k PRL), generated from the cleavage of the 23 kDa prolactin hormone by matrix metalloproteases and cathepsin D in various tissues and cell types. 16k PRL down-regulates the Ras-Tiaml-Rac1-Pakl signaling pathway by reducing Rac1 activation in response to cell stimuli such as wounding (Lee et al. (2007) Cancer Res 67:11045-11053). In some embodiments, PAK activation is decreased by inhibition of NMDA and/or AMPA receptors. Examples of modulators of AMPA receptors include and are not limited to ketamine, MK801, CNQX (6-cyano-7-nitroquinoxaline-2,3-dione); NBQX (2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione); DNQX (6,7-dinitroquinoxaline-2,3-dione); kynurenic acid; 2,3-dihydroxy-6-nitro-7-sulfamoylbenzo-[f]quinoxaline; PCP or the like. In some embodiments, PAK activation is decreased by inhibition of TrkB activation. In some embodiments, PAK activation is decreased by inhibition of BDNF activation of TrkB. In some embodiments, compounds of Formula I-XV are optionally administered in combination with an antibody to BDNF. In some embodiments, PAK activation is decreased by inhibition of TrkB receptors; NMDA receptors; EphB receptors; adenosine receptors; estrogen receptors; integrins; Rho-family GTPases, including Cdc42, Rac (including but not limited to Rac1 and Rac2), CDK5, PI3 kinases, NCK, PDK1, EKT, GRB2, Chp, TC10, Tcl, and Wrch-1; guanine nucleotide exchange factors (“GEFs”), such as but not limited to GEFT, members of the Dbl family of GEFs, p21-activated kinase interacting exchange factor (PIX), DEF6, Zizimin 1, Vav1, Vav2, Dbs, members of the DOCK180 family, Kalirin-7, and Tiam1; G protein-coupled receptor kinase-interacting protein 1 (GIT1), CIB1, filamin A, Etk/Bmx, and/or binding to FMRP and/or sphingosine.

In some embodiments, compounds of Formula I-XV are optionally administered in combination with a compound that decreases PAK levels in the cell, e.g., a compound that directly or indirectly increases the activity of a guanine exchange factor (GEF) that promotes the active state of a Rho family GTPase, such as an agonist of a GEF that activates a Rho family GTPase, such as but not limited to, Rac or cdc42. Activation of GEFs is also effected by compounds that activate TrkB, NMDA, or EphB receptors.

In some embodiments, a PAK clearance agent is a nucleic acid encoding a GEF that activates a Rho family GTPase, in which the GEF is expressed from a constitutive or inducible promoter. In some embodiments, a guanine nucleotide exchange factor (GEF), such as but not limited to a GEF that activates a Rho family GTPase is overexpressed in cells to increase the activation level of one or more Rho family GTPases and thereby lower the level of PAK in cells. GEFs include, for example, members of the Dbl family of GTPases, such as but not limited to, GEFT, PIX (e.g., alphaPIX, betaPIX), DEF6, Zizimin 1, Vavl, Vav2, Dbs, members of the DOCK180 family, hPEM-2, F1100018, kalirin, Tiaml, STEF, DOCK2, DOCK6, DOCK7, DOCK9, Asf, EhGEF3, or GEF-1. In some embodiments, PAK levels are also reduced by a compound that directly or indirectly enhances expression of an endogenous gene encoding a GEF. A GEF expressed from a nucleic acid construct introduced into cells is in some embodiments a mutant GEF, for example a mutant having enhanced activity with respect to wild type.

The clearance agent is optionally a bacterial toxin such as Salmonella typhinmurium toxin SpoE that acts as a GEF to promote cdc42 nucleotide exchange (Buchwald et al. (2002) EMBO J. 21: 3286-3295; Schlumberger et al. (2003) J. Biological Chem. 278: 27149-27159). Toxins such as SopE, fragments thereof, or peptides or polypeptides having an amino acid sequence at least 80% to 100%, e.g., 85%, 90%, 92%, 93%, 95%, 96%, 97%, 98%, 99%, or any other percent from about 80% to about 100% identical to a sequence of at least five, at least ten, at least twenty, at least thirty, at least forty, at least fifty, at least sixty, at least seventy, at least eighty, at least ninety, or at least 100 contiguous amino acids of the toxin are also optionally used as downregulators of PAK activity. The toxin is optionally produced in cells from nucleic acid constructs introduced into cells.

Modulators of Upstream Regulators of PAKs

In some embodiments, compounds of Formula I-XV are optionally administered in combination with a modulator of an upstream regulator of PAKs. In some embodiments, a modulator of an upstream regulator of PAKs is an indirect inhibitor of PAK. In certain instances, a modulator of an upstream regulator of PAKs is a modulator of PDK1. In some instances, a modulator of PDK1 reduces of inhibits the activity of PDK1. In some instances a PDK1 inhibitor is an antisense compound (e.g., any PDK1 inhibitor described in U.S. Pat. No. 6,124,272, which PDK1 inhibitor is incorporated herein by reference). In some instances, a PDK1 inhibitor is a compound described in e.g., U.S. Pat. Nos. 7,344,870, and 7,041,687, which PDK1 inhibitors are incorporated herein by reference. In some embodiments, an indirect inhibitor of PAK is a modulator of a PI3 kinase. In some instances a modulator of a PI3 kinase is a PI3 kinase inhibitor. In some instances, a PI3 kinase inhibitor is an antisense compound (e.g., any PI3 kinase inhibitor described in WO 2001/018023, which PI3 kinase inhibitors are incorporated herein by reference). In some instances, an inhibitor of a PI3 kinase is 3-morpholino-5-phenylnaphthalen-1(4H)-one (LY294002), or a peptide based covalent conjugate of LY294002, (e.g., SF1126, Semaphore pharmaceuticals). In certain embodiments, an indirect inhibitor of PAK is a modulator of Cdc42. In certain embodiments, a modulator of Cdc42 is an inhibitor of Cdc42. In certain embodiments, a Cdc42 inhibitor is an antisense compound (e.g., any Cdc42 inhibitor described in U.S. Pat. No. 6,410,323, which Cdc42 inhibitors are incorporated herein by reference). In some instances, an indirect inhibitor of PAK is a modulator of GRB2. In some instances, a modulator of GRB2 is an inhibitor of GRB2. In some instances a GRB2 inhibitor is a GRb2 inhibitor described in e.g., U.S. Pat. No. 7,229,960, which GRB2 inhibitor is incorporated by reference herein. In certain embodiments, an indirect inhibitor of PAK is a modulator of NCK. In certain embodiments, an indirect inhibitor of PAK is a modulator of ETK. In some instances, a modulator of ETK is an inhibitor of ETK. In some instances an ETK inhibitor is a compound e.g., α-Cyano-(3,5-di-t-butyl-4-hydroxy)thiocinnamide (AG 879).

In some embodiments, indirect PAK inhibitors act by decreasing transcription and/or translation of PAK. An indirect PAK inhibitor in some embodiments decreases transcription and/or translation of a PAK. For example, in some embodiments, modulation of PAK transcription or translation occurs through the administration of specific or non-specific inhibitors of PAK transcription or translation. In some embodiments, proteins or non-protein factors that bind the upstream region of the PAK gene or the 5′ UTR of a PAK mRNA are assayed for their affect on transcription or translation using transcription and translation assays (see, for example, Baker, et al. (2003) J. Biol. Chem. 278: 17876-17884; Jiang et al. (2006) J. Chromatography A 1133: 83-94; Novoa et al. (1997) Biochemistry 36: 7802-7809; Brandi et al. (2007) Methods Enzymol. 431: 229-267). PAK inhibitors include DNA or RNA binding proteins or factors that reduce the level of transcription or translation or modified versions thereof. In other embodiments, compounds of Formula I-XV are optionally administered in combination with an agent that is a modified form (e.g., mutant form or chemically modified form) of a protein or other compound that positively regulates transcription or translation of PAK, in which the modified form reduces transcription or translation of PAK. In yet other embodiments, a transcription or translation inhibitor is an antagonist of a protein or compound that positively regulates transcription or translation of PAK, or is an agonist of a protein that represses transcription or translation.

Regions of a gene other than those upstream of the transcriptional start site and regions of an mRNA other than the 5′ UTR (such as but not limited to regions 3′ of the gene or in the 3′ UTR of an mRNA, or regions within intron sequences of either a gene or mRNA) also include sequences to which effectors of transcription, translation, mRNA processing, mRNA transport, and mRNA stability bind. In some embodiments, compounds of Formula I-XV are optionally administered in combination with a clearance agent comprising a polypeptide having homology to an endogenous protein that affects mRNA processing, transport, or stability, or is an antagonist or agonist of one or more proteins that affect mRNA processing, transport, or turnover, such that the inhibitor reduces the expression of PAK protein by interfering with PAK mRNA transport or processing, or by reducing the half-life of PAK mRNA. A PAK clearance agents in some embodiments interferes with transport or processing of a PAK mRNA, or by reducing the half-life of a PAK mRNA.

For example, PAK clearance agents decrease RNA and/or protein half-life of a PAK isoform, for example, by directly affecting mRNA and/or protein stability. In certain embodiments, PAK clearance agents cause PAK mRNA and/or protein to be more accessible and/or susceptible to nucleases, proteases, and/or the proteasome. In some embodiments, compounds of Formula I-XV are optionally administered in combination with agents that decrease the processing of PAK mRNA thereby reducing PAK activity. For example, PAK clearance agents function at the level of pre-mRNA splicing, 5′ end formation (e.g. capping), 3′ end processing (e.g. cleavage and/or polyadenylation), nuclear export, and/or association with the translational machinery and/or ribosomes in the cytoplasm. In some embodiments, PAK clearance agents cause a decrease in the level of PAK mRNA and/or protein, the half-life of PAK mRNA and/or protein by at least about 5%, at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 80%, at least about 90%, at least about 95%, or substantially 100%.

In some embodiments, the clearance agent comprises one or more RNAi or antisense oligonucleotides directed against one or more PAK isoform RNAs. In some embodiments, compounds of Formula I-XV are optionally administered in combination with agent that comprise one or more ribozymes directed against one or more PAK isoform RNAs. The design, synthesis, and use of RNAi constructs, antisense oligonucleotides, and ribozymes are found, for example, in Dykxhoorn et al. (2003) Nat. Rev. Mol. Cell. Biol. 4: 457-467; Hannon et al. (2004) Nature 431: 371-378; Sarver et al. (1990) Science 247:1222-1225; Been et al. (1986) Cell 47:207-216). In some embodiments, nucleic acid constructs that induce triple helical structures are also introduced into cells to inhibit transcription of the PAK gene (Helene (1991) Anticancer Drug Des. 6:569-584).

For example, a clearance agent is in some embodiments an RNAi molecule or a nucleic acid construct that produces an RNAi molecule. An RNAi molecule comprises a double-stranded RNA of at least about seventeen bases having a 2-3 nucleotide single-stranded overhangs on each end of the double-stranded structure, in which one strand of the double-stranded RNA is substantially complementary to the target PAK RNA molecule whose downregulation is desired. “Substantially complementary” means that one or more nucleotides within the double-stranded region are not complementary to the opposite strand nucleotide(s). Tolerance of mismatches is optionally assessed for individual RNAi structures based on their ability to downregulate the target RNA or protein. In some embodiments, RNAi is introduced into the cells as one or more short hairpin RNAs (“shRNAs”) or as one or more DNA constructs that are transcribed to produce one or more shRNAs, in which the shRNAs are processed within the cell to produce one or more RNAi molecules.

Nucleic acid constructs for the expression of siRNA, shRNA, antisense RNA, ribozymes, or nucleic acids for generating triple helical structures are optionally introduced as RNA molecules or as recombinant DNA constructs. DNA constructs for reducing gene expression are optionally designed so that the desired RNA molecules are expressed in the cell from a promoter that is transcriptionally active in mammalian cells, such as, for example, the SV40 promoter, the human cytomegalovirus immediate-early promoter (CMV promoter), or the pol III and/or pol II promoter using known methods. For some purposes, it is desirable to use viral or plasmid-based nucleic acid constructs. Viral constructs include but are not limited to retroviral constructs, lentiviral constructs, or based on a pox virus, a herpes simplex virus, an adenovirus, or an adeno-associated virus (AAV).

In other embodiments, compounds of Formula I-XV are optionally administered in combination with a polypeptide that decreases the activity of PAK. Protein and peptide inhibitors of PAK are optionally based on natural substrates of PAK, e.g., Myosin light chain kinase (MLCK), regulatory Myosin light chain (R-MLC), Myosins I heavy chain, myosin II heavy chain, Myosin VI, Caldesmon, Desmin, Op18/stathmin, Merlin, Filamin A, LIM kinase (LIMK), cortactin, cofilin, Ras, Raf, Mek, p47(phox), BAD, caspase 3, estrogen and/or progesterone receptors, NET1, Gαz, phosphoglycerate mutase-B, RhoGDI, prolactin, p41Arc, cortactin and/or Aurora-A. In some embodiments, compounds of Formula I-XV are optionally administered in combination with an agent that is based on a sequence of PAK itself, for example, the autoinhibitory domain in the N-terminal portion of the PAK protein that binds the catalytic domain of a partner PAK molecule when the PAK molecule is in its homodimeric state (Zhao et al. (1998) Mol. Cell. Biol. 18:2153-2163; Knaus et al. (1998) J. Biol. Chem. 273: 21512-21518; Hofman et al. (2004) J. Cell Sci. 117: 4343-4354). In some embodiments, polypeptide inhibitors of PAK comprise peptide mimetics, in which the peptide has binding characteristics similar to a natural binding partner or substrate of PAK.

In some embodiments, provided herein are compounds that downregulate PAK protein level. In some embodiments, the compounds described herein activate or increase the activity of an upstream regulator or downstream target of PAK. In some embodiments, compounds described herein downregulate protein level of a PAK. In some instances compounds described herein reduce at least one of the symptoms related a CNS disorder by reducing the amount of PAK in a cell. In some embodiments a compound that decreases PAK protein levels in cells also decreases the activity of PAK in the cells. In some embodiments a compound that decreases PAK protein levels does not have a substantial impact on PAK activity in cells. In some embodiments a compound that increases PAK activity in cells decreases PAK protein levels in the cells.

In some embodiments, a compound that decreases the amount of PAK protein in cells decreases transcription and/or translation of PAK or increases the turnover rate of PAK mRNA or protein by modulating the activity of an upstream effector or downstream regulator of PAK. In some embodiments, PAK expression or PAK levels are influenced by feedback regulation based on the conformation, chemical modification, binding status, or activity of PAK itself. In some embodiments, PAK expression or PAK levels are influenced by feedback regulation based on the conformation, chemical modification, binding status, or activity of molecules directly or indirectly acted on by PAK signaling pathways. As used herein “binding status” refers to any or a combination of whether PAK, an upstream regulator of PAK, or a downstream effector of PAK is in a monomeric state or in an oligomeric complex with itself, or whether it is bound to other polypeptides or molecules. For example, a downstream target of PAK, when phosphorylated by PAK, in some embodiments directly or indirectly downregulates PAK expression or decrease the half-life of PAK mRNA or protein. Downstream targets of PAK include but are not limited to: Myosin light chain kinase (MLCK), regulatory Myosin light chain (R-MLC), Myosins I heavy chain, myosin II heavy chain, Myosin VI, Caldesmon, Desmin, Op18/stathmin, Merlin, Filamin A, LIM kinase (LIMK), Ras, Raf, Mek, p47^(phox), BAD, caspase 3, estrogen and/or progesterone receptors, NET1, Gαz, phosphoglycerate mutase-B, RhoGDI, prolactin, p41^(Arc), cortactin and/or Aurora-A. Downregulators of PAK levels include downstream targets of PAK or fragments thereof in a phosphorylated state and downstream targets of PAK or fragments thereof in a hyperphosphorylated state.

A fragment of a downstream target of PAK includes any fragment with an amino acid sequence at least 80% to 100%, e.g., 85%, 90%, 92%, 93%, 95%, 96%, 97%, 98%, 99%, or any other percent from about 80% to about 100% identical to a sequence of at least five, at least ten, at least twenty, at least thirty, at least forty, at least fifty, at least sixty, at least seventy, at least eighty, at least ninety, or at least 100 contiguous amino acids of the downstream regulator, in which the fragment of the downstream target of PAK is able to downregulate PAK mRNA or protein expression or increase turnover of PAK mRNA or protein. In some embodiments, the fragment of a downstream regulator of PAK comprises a sequence that includes a phosphorylation site recognized by PAK, in which the site is phosphorylated.

In some embodiments, compounds of Formula I-XV are optionally administered in combination with a compound that decreases the level of PAK including a peptide, polypeptide, or small molecule that inhibits dephosphorylation of a downstream target of PAK, such that phosphorylation of the downstream target remains at a level that leads to downregulation of PAK levels.

In some embodiments, PAK activity is reduced or inhibited via activation and/or inhibition of an upstream regulator and/or downstream target of PAK. In some embodiments, the protein expression of a PAK is downregulated. In some embodiments, the amount of PAK in a cell is decreased. In some embodiments a compound that decreases PAK protein levels in cells also decreases the activity of PAK in the cells. In some embodiments a compound that decreases PAK protein levels does not decrease PAK activity in cells. In some embodiments a compound that increases PAK activity in cells decreases PAK protein levels in the cells.

In some instances, compounds of Formula I-XV are optionally administered in combination with a polypeptide that is delivered to one or more brain regions of an individual by administration of a viral expression vector, e.g., an AAV vector, a lentiviral vector, an adenoviral vector, or a HSV vector. A number of viral vectors for delivery of therapeutic proteins are described in, e.g., U.S. Pat. Nos. 7,244,423, 6,780,409, 5,661,033. In some embodiments, the PAK inhibitor polypeptide to be expressed is under the control of an inducible promoter (e.g., a promoter containing a tet-operator). Inducible viral expression vectors include, for example, those described in U.S. Pat. No. 6,953,575. Inducible expression of a PAK inhibitor polypeptide allows for tightly controlled and reversible increases of PAK inhibitor polypeptide expression by varying the dose of an inducing agent (e.g., tetracycline) administered to an individual.

Anti-Cancer Agents

Where the subject is suffering from or at risk of suffering from a cell proliferative disorder (e.g., cancer), the subject in some embodiments is treated with a compound of Formula I-VIII in any combination with one or more anti-cancer therapy(ies). In some embodiments, one or more of the anti-cancer therapy(ies) comprises surgery, chemotherapy, radiation therapy, photodynamic therapy, gene therapy or immunotherapy. In some embodiments, the anti-cancer therapy comprises surgery, wherein the cancer or a portion thereof is physically removal from a subject. In some instances, the disease type, stage and the individual's age and condition will determine what type of surgery may be performed. In some instances, the anti-cancer therapy comprises chemotherapy. In some instances, the chemotherapy uses drugs to kill cancer cells. In some instances, these drugs target rapidly-dividing cells and attempt to inhibit this division of cells. In some embodiments, the anti-cancer therapy comprises radiation therapy. In some instances, radiation therapy uses high-energy x-rays help to destroy cancer cells and shrink tumors. In some instances, the radiation is from outside the body from a machine (e.g., external radiation). In other instances, the radiation is from radioactive materials placed directly in or around cancer cells through thin plastic tubes (e.g., internal or implant radiation). In some embodiments, the anti-cancer therapy comprises photodynamic therapy. In some instances, photodynamic therapy destroys cancer cells by using the energy from light and may also be effective when combined with surgery. In some instances, the anti-cancer therapy comprises gene therapy. This approach allows treatment to target tumors, rather than destroying healthy cells. In some embodiments, the anti-cancer therapy comprises immunotherapy. Immunotherapy (or biological therapy) treats cancer by using the body's own immune system to fight cancer cells. Another name often applies to this therapy: biological response modifiers (BRMs).

Disclosed herein is a method for treating a tumor associated with neurofibromatosis (NF) in a subject comprising administering a PAK inhibitor to the subject. Further disclosed herein is a method for treating a tumor associated with neurofibromatosis (NF) in a subject comprising administering two or more agents to the subject, wherein at least of the agents is a PAK inhibitor. In some embodiments, the neurofibromatosis is type 1 neurofibromatosis. In some embodiments, the neurofibromatosis is type 2 neurofibromatosis. In some embodiments, the method further comprises administering an anti-cancer agent. In some embodiments, the second agent is an anti-cancer agent. In some embodiments, the anti-cancer agent is an mTOR inhibitor, VEGF inhibitor. In some embodiments, the mTOR inhibitor is rapamycin or everolimus. In some embodiments, the anti-cancer agent is selected from AZD2171, AZD6244 hydrogen sulfate, bevacizumab, PTC299, pirfenidone, sorafenib, sirolimus, imiquimod, lapatinib, nilotinib, sunitinib, sunitinib malate, AMN107, PEG-Intron, or any combination thereof. In some embodiments, the second agent is anti-neurofibromatosis agent. In some instances, the anti-neurofibromatosis agent is lovastatin. In some embodiments, the method further comprises administering a proton therapy. In other embodiments, the method further comprises administering a photodynamic therapy. In some embodiments, the photodynamic therapy comprises LS11. In some embodiments, the method further comprises administering radiation therapy.

Disclosed herein is a method for treating a mesothelioma in a subject comprising administering a PAK inhibitor to the subject. Further disclosed herein is a method for treating a mesothelioma in a subject comprising administering two or more agents to the subject, wherein at least one of the agents is a PAK inhibitor. In some embodiments, the mesothelioma is a malignant mesothelioma. In some embodiments, the method further comprises administering an anti-cancer agent. In some embodiments, the second agent is an anti-cancer agent. In some embodiments, the anti-cancer agent is selected from everolimus, cisplatin, imatinib mesylate, pemetrexed, erlotinib, bevacizumab, dasatininb, ZD1839, semaxanib, gefitinib, gemcitabine, amifostine, sodium thiosulfate, mitomycin C, vinblastine sulfate, vinorlbine tartrate, ganciclovir, raltitrexed, carboplatin, doxorubicin, onconase, vorinstat, bortezomib, pazopanib, capecitabine, vatalanib, rilotumumab, trabectedin, GC1008, zoledronic acid, PF-03446962, or any combination thereof. In some embodiments, the method further comprises administering pentostatin, cyclphosphamide and SS1P. In some embodiments, the method further comprises administering oxaliplatin and gemcitabine. In some embodiments, the method further comprises administering carboplatin. In some embodiments, the method further comprises administering valproate and doxorubicin.

Disclosed herein is a method for treating a meningioma in a subject comprising administering a PAK inhibitor to the subject. Further disclosed herein is a method for treating a meningioma in a subject comprising administering two or more agents to the subject, wherein at least one of the agents is a PAK inhibitor. In some embodiments, the meningioma is a recurrent or inoperable meningioma. In some embodiments, the meningioma is a refractory meningioma. In some embodiments, the method further comprises administering an anti-cancer agent. In some embodiments, the second agent is an anti-cancer agent. In some embodiments, the anti-cancer agent is selected from sunitinib, sunitinib malate, SOM230C, SOM230B, hydroxurea, vatalinib, verapamil, imatinib mesylate, everolimus, bevacizumab, panobinostat, erlotinib, erlotinib hydrochloride, gefitinib, pioglitazone, ifosfamide, lapatinib, entinostat, ixabepilone, topotecan hydrochloride, enzastaurin hydrochloride, sodium phenylbutyrate, temozolomide, carboplatin, talabostat mesylate, taIotrexin, busulfan, semaxinib, filgrastim, pegfilgrastim, trabectedin, 06-benzylguanine, temozolomide, ABT-751, romidepsin, AZD2171, thalidomide, crizotinib, ispinesib, cilengitide or any combination thereof. In some embodiments, the method further comprises administering radiation therapy. In some embodiments, the radiation therapy comprises carbon ion radiotherapy, proton radiation, proton beam radiation therapy, or intensity-modulated radiation therapy. In some embodiments, the method further comprises stereotactic radiosurgery. In some embodiments, the method further comprises administering hydroxyurea and verapamil. In some embodiments, the method further comprises administering everolimus and bevacizumab.

Disclosed herein is a method for treating a glioma in a subject comprising administering a PAK inhibitor to the subject. Further disclosed herein is a method for treating a glioma in a subject comprising administering two or more agents to the subject, wherein at least one of the agents is a PAK inhibitor. In some embodiments, the glioma is a malignant glioma. In some embodiments, the glioma is a high grade glioma or supratentorial high-grade glioma. In some embodiments, the glioma is a diffuse intrinsic pontine glioma. In some embodiments, the glioma is a recurrent glioma. In some embodiments, the method further comprises administering an anti-cancer agent. In some embodiments, the second agent is an anti-cancer agent. In some embodiments, the anti-cancer agent is selected from temozolomide, bevacizumab, irinotecan, talasporfin sodium, erlotinib hydrochloride, cilengitide, crenolanib, naltrexone, IL13-PE38QQR, AZD6244, XL765, AZD8055, 131-I-TM-601, ANG1005, vandetanib, everolimus, valproic acid, PEG-interferon alpha-2B, 2B3-101, ritnoavir, lopinavir, carboplatin, dichloroacetate, thalomid, or any combination thereof. In some embodiments, the method further comprises radiation therapy. In some embodiments, the method further comprises administering bevacizumab, Gleevac®, everolimus, or any combination thereof. In some embodiments, the radiation therapy is selected from intensity modulated radiation therapy (IMRT), sterotactic radiosurgery (SRS), intraoperative radiation therapy (IORT), image guided radiation therapy (IGRT). In some embodiments, the method further comprises immunotherapy or targeted therapy. In some embodiments, the method further comprises vaccine therapy. In some embodiments, the vaccine therapy comprises DCVax®-L.

Disclosed herein is a method for treating a schwannoma in a subject comprising administering a PAK inhibitor to the subject. Further disclosed herein is a method for treating a schwannoma in a subject comprising administering two or more agents to the subject, wherein at least one of the agents is a PAK inhibitor. In some embodiments, the method further comprises administering an anti-cancer agent. In some embodiments, the second agent is an anti-cancer agent. In some embodiments, the anti-cancer agent is selected from bevacizumab, everolimus, RAD001, lapatinib, nilotinib, Afinitor®, pazopanib, ifosfamide, dasatinib, sorafenib, dacarbazine, erlotinib, erlotinib hydrochloride, imatinib mesylate, or any combination thereof. In some embodiments, the method further comprises administering gemcitabine and docetaxel. In some embodiments, the method further comprises radiation therapy. In some instances, the radiation therapy is selected from stereotactic radiotherapy, fractionated proton radiation. In some embodiments, the method further comprises proton therapy or surgery.

Disclosed herein is a method for treating a Jung cancer in a subject comprising administering a PAK inhibitor to the subject. Further disclosed herein is a method for treating a lung cancer in a subject comprising administering two or more agents to the subject, wherein at least one of the NSCLC is an advanced NSCLC. In other embodiments, the lung cancer is SCLC. In some embodiments, the method further comprises adminikering an anti-cancer agent. In some embodiments, the second agent is an anti-cancer agent. In some embodiments, the anti-cancer agent is selected from cisplatin, gemcitabine, pemetrexed, docetaxel, vinorelbine, or any combination thereof. In some embodiments, the anti-cancer agent is endostar, vorinostat, nimotuzumab, carboplatin, mapatumumab, paclitaxel, BIBW2992, ISIS EIF4E, figitumumab, erlotinib, cabazitaxel-XRP6258, GRN1005, panitumumab, AMG 706, dasatinib, epirubicin, NRX194204, vandetanib, ARQ197, Lacanix™, or any combination thereof. In some embodiments, the method further comprises radiation therapy, endobronchial therapy, surgery, chemotherapy, or any combination thereof. In some embodiments, radiation therapy comprises conformational radiotherapy, proton radiotherapy, thermal ablation with external beam radiation, or any combination thereof. In some embodiments, endobronchial therapy comprises photodynamic therapy. In some embodiments, the method further comprises vaccine therapy. In some embodiments, the vaccine therapy comprises a recombinant human rEGF-P64K/montanide vaccine.

Where the subject is suffering from or at risk of suffering from a cell proliferative disorder (e.g., plasma cell myeloma, glioma, mesothelioma, neurofibromatosis, schwannoma, breast cancer, NSCLC, SCLC, ovarian cancer, head and neck cancer, and esophageal squamous cancer), the subject in some embodiments is treated with a compound of Formula I-XV in any combination with one or more other anti-cancer agents. In some embodiments, one or more of the anti-cancer agents are proapoptotic agents. Examples of anti-cancer agents include, but are not limited to, any of the following: gossyphol, genasense, polyphenol E, Chlorofusin, all trans-retinoic acid (ATRA), bryostatin, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), 5-aza-2′-deoxycytidine, all trans retinoic acid, doxorubicin, vincristine, etoposide, gemcitabine, imatinib (Gleevec®), geldanamycin, 17-N-Allylamino-17-Demethoxygeldanamycin (17-AAG), flavopiridol, LY294002, bortezomib, trastuzumab, BAY 11-7082, PKC412, or PD184352, Taxol™, also referred to as “paclitaxel”, which is an anti-cancer drug which acts by enhancing and stabilizing microtubule formation, and analogs of Taxol™, such as Taxotere™. Compounds that have the basic taxane skeleton as a common structure feature, have also been shown to have the ability to arrest cells in the G2-M phases due to stabilized microtubules and in some embodiments are useful for treating cancer in combination with the compounds described herein.

Further examples of anti-cancer agents for use in combination with a compound of Formula I-XV include inhibitors of mitogen-activated protein kinase signaling, e.g., U0126, PD98059, PD184352, PD0325901, ARRY-142886, SB239063, SP600125, BAY 43-9006, wortmannin, or LY294002; Syk inhibitors; mTOR inhibitors; and antibodies (e.g., rituxan).

Other anti-cancer agents that can be employed in combination with an irreversible Btk inhibitor compound include Adriamycin, Dactinomycin, Bleomycin, Vinblastine, Cisplatin, acivicin; aclarubicin; acodazole hydrochloride; acronine; adozelesin; aldesleukin; altretamine; ambomycin; ametantrone acetate; aminoglutethimide; amsacrine; anastrozole; anthramycin; asparaginase; asperlin; azacitidine; azetepa; azotomycin; batimastat; benzodepa; bicalutamide; bisantrene hydrochloride; bisnafide dimesylate; bizelesin; bleomycin sulfate; brequinar sodium; bropirimine; busulfan; cactinomycin; calusterone; caracemide; carbetimer; carboplatin; carmustine; carubicin hydrochloride; carzelesin; cedefingol; chlorambucil; cirolemycin; cladribine; crisnatol mesylate; cyclophosphamide; cytarabine; dacarbazine; daunorubicin hydrochloride; decitabine; dexormaplatin; dezaguanine; dezaguanine mesylate; diaziquone; doxorubicin; doxorubicin hydrochloride; droloxifene; droloxifene citrate; dromostanolone propionate; duazomycin; edatrexate; eflornithine hydrochloride; elsamitrucin; enloplatin; enpromate; epipropidine; epirubicin hydrochloride; erbulozole; esorubicin hydrochloride; estramustine; estramustine phosphate sodium; etanidazole; etoposide; etoposide phosphate; etoprine; fadrozole hydrochloride; fazarabine; fenretinide; floxuridine; fludarabine phosphate; fluorouracil; fluorocitabine; fosquidone; fostriecin sodium; gemcitabine; gemcitabine hydrochloride; hydroxyurea; idarubicin hydrochloride; ifosfamide; ilmofosine; interleukin II (including recombinant interleukin II, or rIL2), interferon alfa-2a; interferon alfa-2b; interferon alfa-n1; interferon alfa-n3; interferon beta-1a; interferon gamma-1b; iproplatin; irinotecan hydrochloride; lanreotide acetate; letrozole; leuprolide acetate; liarozole hydrochloride; lometrexolsodium; lomustine; losoxantrone hydrochloride; masoprocol; maytansine; mechlorethamine hydrochloride; megestrol acetate; melengestrol acetate; melphalan; menogaril; mercaptopurine; methotrexate; methotrexate sodium; metoprine; meturedepa; mitindomide; mitocarcin; mitocromin; mitogillin; mitomalcin; mitomycin; mitosper; mitotane; mitoxantrone hydrochloride; mycophenolic acid; nocodazole; nogalamycin; ormaplatin; oxisuran; pegaspargase; peliomycin; pentamustine; peplomycin sulfate; perfosfamide; pipobroman; piposulfan; piroxantrone hydrochloride; plicamycin; plomestane; porfimer sodium; porfiromycin; prednimustine; procarbazine hydrochloride; puromycin; puromycin hydrochloride; pyrazofurin; riboprine; rogletimide; safingol; safingol hydrochloride; semustine; simtrazene; sparfosate sodium; sparsomycin; spirogermanium hydrochloride; spiromustine; spiroplatin; streptonigrin; streptozocin; sulofenur; talisomycin; tecogalan sodium; tegafur; teloxantrone hydrochloride; temoporfin; teniposide; teroxirone; testolactone; thiamiprine; thioguanine; thiotepa; tiazofurin; tirapazamine; toremifene citrate; trestolone acetate; triciribine phosphate; trimetrexate; trimetrexate glucuronate; triptorelin; tubulozole hydrochloride; uracil mustard; uredepa; vapreotide; verteporfin; vinblastine sulfate; vincristine sulfate; vindesine; vindesine sulfate; vinepidine sulfate; vinglycinate sulfate; vinleurosine sulfate; vinorelbine tartrate; vinrosidine sulfate; vinzolidine sulfate; vorozole; zeniplatin; zinostatin; zorubicin hydrochloride.

Other anti-cancer agents that in some embodiments are employed in combination with a compound of Formula I-XV include: 20-epi-1,25 dihydroxyvitamin D3; 5-ethynyluracil; abiraterone; aclarubicin; acylfulvene; adecypenol; adozelesin; aldesleukin; ALL-TK antagonists; altretamine; ambamustine; amidox; amifostine; aminolevulinic acid; amrubicin; amsacrine; anagrelide; anastrozole; andrographolide; angiogenesis inhibitors; antagonist D; antagonist G; antarelix; anti-dorsalizing morphogenetic protein-1; antiandrogen, prostatic carcinoma; antiestrogen; antineoplaston; antisense oligonucleotides; aphidicolin glycinate; apoptosis gene modulators; apoptosis regulators; apurinic acid; ara-CDP-DL-PTBA; arginine deaminase; asulacrine; atamestane; atrimustine; axinastatin 1; axinastatin 2; axinastatin 3; azasetron; azatoxin; azatyrosine; baccatin III derivatives; balanol; batimastat; BCR/ABL antagonists; benzochlorins; benzoylstaurosporine; beta lactam derivatives; beta-alethine; betaclamycin B; betulinic acid; bFGF inhibitor; bicalutamide; bisantrene; bisaziridinylspermine; bisnafide; bistratene A; bizelesin; breflate; bropirimine; budotitane; buthionine sulfoximine; calcipotriol; calphostin C; camptothecin derivatives; canarypox IL-2; capecitabine; carboxamide-amino-triazole; carboxyamidotriazole; CaRest M3; CARN 700; cartilage derived inhibitor; carzelesin; casein kinase inhibitors (ICOS); castanospermine; cecropin B; cetrorelix; chlorins; chloroquinoxaline sulfonamide; cicaprost; cis-porphyrin; cladribine; clomifene analogues; clotrimazole; collismycin A; collismycin B; combretastatin A4; combretastatin analogue; conagenin; crambescidin 816; crisnatol; cryptophycin 8; cryptophycin A derivatives; curacin A; cyclopentanthraquinones; cycloplatam; cypemycin; cytarabine ocfosfate; cytolytic factor; cytostatin; dacliximab; decitabine; dehydrodidemnin B; deslorelin; dexamethasone; dexifosfamide; dexrazoxane; dexverapamil; diaziquone; didemnin B; didox; diethylnorspermine; dihydro-5-azacytidine; 9-dioxamycin; diphenyl spiromustine; docosanol; dolasetron; doxifluridine; droloxifene; dronabinol; duocarmycin SA; ebselen; ecomustine; edelfosine; edrecolomab; eflomithine; elemene; emitefur; epirubicin; epristeride; estramustine analogue; estrogen agonists; estrogen antagonists; etanidazole; etoposide phosphate; exemestane; fadrozole; fazarabine; fenretinide; filgrastim; fmasteride; flavopiridol; flezelastine; fluasterone; fludarabine; fluorodaunorunicin hydrochloride; forfenimex; formestane; fostriecin; fotemustine; gadolinium texaphyrin; gallium nitrate; galocitabine; ganirelix; gelatinase inhibitors; gemcitabine; glutathione inhibitors; hepsulfam; heregulin; hexamethylene bisacetamide; hypericin; ibandronic acid; idarubicin; idoxifene; idramantone; ilmofosine; ilomastat; imidazoacridones; imiquimod; immunostimulant peptides; insulin-like growth factor-1 receptor inhibitor; interferon agonists; interferons; interleukins; iobenguane; iododoxorubicin; ipomeanol, 4-; iroplact; irsogladine; isobengazole; isohomohalicondrin B; itasetron; jasplakinolide; kahalalide F; lamellarin-N triacetate; lanreotide; leinamycin; lenograstim; lentinan sulfate; leptolstatin; letrozole; leukemia inhibiting factor; leukocyte alpha interferon; leuprolide+estrogen+progesterone; leuprorelin; levamisole; liarozole; linear polyamine analogue; lipophilic disaccharide peptide; lipophilic platinum compounds; lissoclinamide 7; lobaplatin; lombricine; lometrexol; lonidamine; losoxantrone; lovastatin; loxoribine; lurtotecan; lutetium texaphyrin; lysofylline; lytic peptides; maitansine; mannostatin A; marimastat; masoprocol; maspin; matrilysin inhibitors; matrix metalloproteinase inhibitors; menogaril; merbarone; meterelin; methioninase; metoclopramide; MIF inhibitor; mifepristone; miltefosine; mirimostim; mismatched double stranded RNA; mitoguazone; mitolactol; mitomycin analogues; mitonafide; mitotoxin fibroblast growth factor-saporin; mitoxantrone; mofarotene; molgramostim; monoclonal antibody, human chorionic gonadotrophin; monophosphoryl lipid A+myobacterium cell wall sk; mopidamol; multiple drug resistance gene inhibitor; multiple tumor suppressor 1-based therapy; mustard anticancer agent; mycaperoxide B; mycobacterial cell wall extract; myriaporone; N-acetyldinaline; N-substituted benzamides; nafarelin; nagrestip; naloxone+pentazocine; napavin; naphterpin; nartograstim; nedaplatin; nemorubicin; neridronic acid; neutral endopeptidase; nilutamide; nisamycin; nitric oxide modulators; nitroxide antioxidant; nitrullyn; O6-benzylguanine; octreotide; okicenone; oligonucleotides; onapristone; ondansetron; ondansetron; oracin; oral cytokine inducer; ormaplatin; osaterone; oxaliplatin; oxaunomycin; palauamine; palmitoylrhizoxin; pamidronic acid; panaxytriol; panomifene; parabactin; pazelliptine; pegaspargase; peldesine; pentosan polysulfate sodium; pentostatin; pentrozole; perflubron; perfosfamide; perillyl alcohol; phenazinomycin; phenylacetate; phosphatase inhibitors; picibanil; pilocarpine hydrochloride; pirarubicin; piritrexim; placetin A; placetin B; plasminogen activator inhibitor; platinum complex; platinum compounds; platinum-triamine complex; porfimer sodium; porfiromycin; prednisone; propyl bis-acridone; prostaglandin J2; proteasome inhibitors; protein A-based immune modulator; protein kinase C inhibitor; protein kinase C inhibitors, microalgal; protein tyrosine phosphatase inhibitors; purine nucleoside phosphorylase inhibitors; purpurins; pyrazoloacridine; pyridoxylated hemoglobin polyoxyethylerie conjugate; raf antagonists; raltitrexed; ramosetron; ras farnesyl protein transferase inhibitors; ras inhibitors; ras-GAP inhibitor; retelliptine demethylated; rhenium Re 186 etidronate; rhizoxin; ribozymes; R.sub.11 retinamide; rogletimide; rohitukine; romurtide; roquinimex; rubiginone B1; ruboxyl; safingol; saintopin; SarCNU; sarcophytol A; sargramostim; Sdi 1 mimetics; semustine; senescence derived 1; sense oligonucleotides; signal transduction inhibitors; signal transduction modulators; single chain antigen-binding protein; sizofuran; sobuzoxane; sodium borocaptate; sodium phenylacetate; solverol; somatomedin binding protein; sonermin; sparfosic acid; spicamycin D; spiromustine; splenopentin; spongistatin 1; squalamine; stem cell inhibitor; stem-cell division inhibitors; stipiamide; stromelysin inhibitors; sulfinosine; superactive vasoactive intestinal peptide antagonist; suradista; suramin; swainsonine; synthetic glycosaminoglycans; tallimustine; tamoxifen methiodide; tauromustine; tazarotene; tecogalan sodium; tegafur; tellurapyrylium; telomerase inhibitors; temoporfin; temozolomide; teniposide; tetrachlorodecaoxide; tetrazomine; thaliblastine; thiocoraline; thrombopoietin; thrombopoietin mimetic; thymalfasin; thymopoietin receptor agonist; thymotrinan; thyroid stimulating hormone; tin ethyl etiopurpurin; tirapazamine; titanocene bichloride; topsentin; toremifene; totipotent stem cell factor; translation inhibitors; tretinoin; triacetyluridine; triciribine; trimetrexate; triptorelin; tropisetron; turosteride; tyrosine kinase inhibitors; tyrphostins; UBC inhibitors; ubenimex; urogenital sinus-derived growth inhibitory factor; urokinase receptor antagonists; vapreotide; variolin B; vector system, erythrocyte gene therapy; velaresol; veramine; verdins; verteporfin; vinorelbine; vinxaltine; vitaxin; vorozole; zanoterone; zeniplatin; zilascorb; and zinostatin stimalamer.

Yet other anticancer agents that in further embodiments are employed in combination with a compound of Formula I-XV include alkylating agents, antimetabolites, natural products, or hormones, e.g., nitrogen mustards (e.g., mechloroethamine, cyclophosphamide, chlorambucil, etc.), alkyl sulfonates (e.g., busulfan), nitrosoureas (e.g., carmustine, lomusitne, etc.), or triazenes (decarbazine, etc.). Examples of antimetabolites include but are not limited to folic acid analog (e.g., methotrexate), or pyrimidine analogs (e.g., Cytarabine), purine analogs (e.g., mercaptopurine, thioguanine, pentostatin).

Examples of natural products useful in combination with a compound of Formula I-XV include but are not limited to vinca alkaloids (e.g., vinblastin, vincristine), epipodophyllotoxins (e.g., etoposide), antibiotics (e.g., daunorubicin, doxorubicin, bleomycin), enzymes (e.g., L-asparaginase), or biological response modifiers (e.g., interferon α).

Examples of alkylating agents that in further embodiments are employed in combination with a compound of Formula I-XV include, but are not limited to, nitrogen mustards (e.g., mechloroethamine, cyclophosphamide, chlorambucil, melphalan, etc.), ethylenimine and methylmelamines (e.g., hexamethlymelamine, thiotepa), alkyl sulfonates (e.g., busulfan), nitrosoureas (e.g., carmustine, lomusitne, semustine, streptozocin, etc.), or triazenes (decarbazine, etc.). Examples of antimetabolites include, but are not limited to folic acid analog (e.g., methotrexate), or pyrimidine analogs (e.g., fluorouracil, floxuridine, Cytarabine), purine analogs (e.g., mercaptopurine, thioguanine, pentostatin.

Examples of hormones and antagonists useful in combination with a compound of Formula I-XV include, but are not limited to, adrenocorticosteroids (e.g., prednisone), progestins (e.g., hydroxyprogesterone caproate, megestrol acetate, medroxyprogesterone acetate), estrogens (e.g., diethlystilbestrol, ethinyl estradiol), antiestrogen (e.g., tamoxifen), androgens (e.g., testosterone propionate, fluoxymesterone), antiandrogen (e.g., flutamide), gonadotropin releasing hormone analog (e.g., leuprolide). Other agents that can be used in the methods and compositions described herein for the treatment or prevention of cancer include platinum coordination complexes (e.g., cisplatin, carboblatin), anthracenedione (e.g., mitoxantrone), substituted urea (e.g., hydroxyurea), methyl hydrazine derivative (e.g., procarbazine), adrenocortical suppressant (e.g., mitotane, aminoglutethimide).

Examples of anti-cancer agents which act by arresting cells in the G2-M phases due to stabilized microtubules and which in other embodiments are used in combination with a compound of Formula I-XV include without limitation the following marketed drugs and drugs in development: Erbulozole (also known as R-55104), Dolastatin 10 (also known as DLS-10 and NSC-376128), Mivobulin isethionate (also known as CI-980), Vincristine, NSC-639829, Discodermolide (also known as NVP-XX-A-296), ABT-751 (Abbott, also known as E-7010), Altorhyrtins (such as Altorhyrtin A and Altorhyrtin C), Spongistatins (such as Spongistatin 1, Spongistatin 2, Spongistatin 3, Spongistatin 4, Spongistatin 5, Spongistatin 6, Spongistatin 7, Spongistatin 8, and Spongistatin 9), Cemadotin hydrochloride (also known as LU-103793 and NSC-D-669356), Epothilones (such as Epothilone A, Epothilone B, Epothilone C (also known as desoxyepothilone A Or dEpoA), Epothilone D (also referred to as KOS-862, dEpoB, and desoxyepothilone B), Epothilone E, Epothilone F, Epothilone B N-oxide, Epothilone A N-oxide, 16-aza-epothilone B, 21-aminoepothilone B (also known as BMS-310705), 21-hydroxyepothilone D (also known as Desoxyepothilone F and dEpoF), 26-fluoroepothilone), Auristatin PE (also known as NSC-654663), Soblidotin (also known as TZT-1027), LS-4559-P (Pharmacia, also known as LS-4577), LS-4578 (Pharmacia, also known as LS-477-P), LS-4477 (Pharmacia), LS-4559 (Pharmacia), RPR-112378 (Aventis), Vincristine sulfate, DZ-3358 (Daiichi), FR-182877 (Fujisawa, also known as WS-9885B), GS-164 (Takeda), GS-198 (Takeda), KAR-2 (Hungarian Academy of Sciences), BSF-223651 (BASF, also known as ILX-651 and LU-223651), SAH-49960 (Lilly/Novartis), SDZ-268970 (Lilly/Novartis), AM-97 (Armad/Kyowa Hakko), AM-132 (Armad), AM-138 (Armad/Kyowa Hakko), IDN-5005 (Indena), Cryptophycin 52 (also known as LY-355703), AC-7739 (Ajinomoto, also known as AVE-8063A and CS-39.HCl), AC-7700 (Ajinomoto, also known as AVE-8062, AVE-8062A, CS-39-L-Ser.HCl, and RPR-258062A), Vitilevuamide, Tubulysin A, Canadensol, Centaureidin (also known as NSC-106969), T-138067 (Tularik, also known as T-67, TL-138067 and TI-138067), COBRA-1 (Parker Hughes Institute, also known as DDE-261 and WHI-261), H10 (Kansas State University), H16 (Kansas State University), Oncocidin A1 (also known as BTO-956 and DIME), DDE-313 (Parker Hughes Institute), Fijianolide B. Laulimalide, SPA-2 (Parker Hughes Institute), SPA-1 (Parker Hughes Institute, also known as SPIKET-P), 3-IAABU (Cytoskeleton/Mt. Sinai School of Medicine, also known as MF-569), Narcosine (also known as NSC-5366), Nascapine, D-24851 (Asta Medica), A-105972 (Abbott), Hemiasterlin, 3-BAABU (Cytoskeleton/Mt. Sinai School of Medicine, also known as MF-191), TMPN (Arizona State University), Vanadocene acetylacetonate, T-138026 (Tularik), Monsatrol, Inanocine (also known as NSC-698666), 3-1AABE (Cytoskeleton/Mt. Sinai School of Medicine), A-204197 (Abbott), T-607 (Tuiarik, also known as T-900607), RPR-115781 (Aventis), Eleutherobins (such as Desmethyleleutherobin, Desaetyleleutherobin, Isoeleutherobin A, and Z-Eleutherobin), Caribaeoside, Caribaeolin, Halichondrin B, D-64131 (Asta Medica), D-68144 (Asta Medica), Diazonamide A, A-293620 (Abbott), NPI-2350 (Nereus), Taccalonolide A, TUB-245 (Aventis), A-259754 (Abbott), Diozostatin, (−)-Phenylahistin (also known as NSCL-96F037), D-68838 (Asta Medica), D-68836 (Asta Medica), Myoseverin B, D-43411 (Zentaris, also known as D-81862), A-289099 (Abbott), A-318315 (Abbott), HTI-286 (also known as SPA-110, trifluoroacetate salt) (Wyeth), D-82317 (Zentaris), D-82318 (Zentaris), SC-12983 (NCI), Resverastatin phosphate sodium, BPR-OY-007 (National Health Research Institutes), and SSR-250411 (Sanofi).

Any combination of one or more PAK inhibitors and a second therapeutic agent is compatible with any method described herein. The PAK inhibitor compositions described herein are also optionally used in combination with other therapeutic reagents that are selected for their therapeutic value for the condition to be treated. In general, the compositions described herein and, in embodiments where combinational therapy is employed, other agents do not have to be administered in the same pharmaceutical composition, and, because of different physical and chemical characteristics, are optionally administered by different routes. The initial administration is generally made according to established protocols, and then, based upon the observed effects, the dosage, modes of administration and times of administration subsequently modified.

In certain instances, it is appropriate to administer at least one PAK inhibitor composition described herein in combination with another therapeutic agent. By way of example only, if one of the side effects experienced by a patient upon receiving one of the PAK inhibitor compositions described herein is nausea, then it is appropriate to administer an anti-nausea agent in combination with the initial therapeutic agent. Or, by way of example only, the therapeutic effectiveness of a PAK inhibitor is enhanced by administration of an adjuvant (i.e., by itself the adjuvant has minimal therapeutic benefit, but in combination with another therapeutic agent, the overall therapeutic benefit to the patient is enhanced). Or, by way of example only, the benefit experienced by a patient is increased by administering a PAK inhibitor with another therapeutic agent (which also includes a therapeutic regimen) that also has therapeutic benefit. In any case, regardless of the disease, disorder or condition being treated, the overall benefit experienced by the patient is either simply additive of the two therapeutic agents or the patient experiences a synergistic benefit.

Therapeutically-effective dosages vary when the drugs are used in treatment combinations. Suitable methods for experimentally determining therapeutically-effective dosages of drugs and other agents include, e.g., the use of metronomic dosing, i.e., providing more frequent, lower doses in order to minimize toxic side effects. Combination treatment further includes periodic treatments that start and stop at various times to assist with the clinical management of the patient.

In any case, the multiple therapeutic agents (one of which is a PAK inhibitor described herein) are administered in any order, or even simultaneously. If simultaneously, the multiple therapeutic agents are optionally provided in a single, unified form, or in multiple forms (by way of example only, either as a single pill or as two separate pills). In some embodiments, one of the therapeutic agents is given in multiple doses, or both are given as multiple doses. If not simultaneous, the timing between the multiple doses optionally varies from more than zero weeks to less than four weeks. In addition, the combination methods, compositions and formulations are not to be limited to the use of only two agents; the use of multiple therapeutic combinations are also envisioned.

The pharmaceutical agents which make up the combination therapy disclosed herein are optionally a combined dosage form or in separate dosage forms intended for substantially simultaneous administration. The pharmaceutical agents that make up the combination therapy are optionally also be administered sequentially, with either therapeutic compound being administered by a regimen calling for two-step administration. The two-step administration regimen optionally calls for sequential administration of the active agents or spaced-apart administration of the separate active agents. The time period between the multiple administration steps ranges from, a few minutes to several hours, depending upon the properties of each pharmaceutical agent, such as potency, solubility, bioavailability, plasma half-life and kinetic profile of the pharmaceutical agent. Circadian variation of the target molecule concentration is optionally used to determine the optimal dose interval.

In addition, a PAK inhibitor is optionally used in combination with procedures that provide additional or synergistic benefit to the patient. By way of example only, patients are expected to find therapeutic and/or prophylactic benefit in the methods described herein, wherein pharmaceutical composition of a PAK inhibitor and/or combinations with other therapeutics are combined with genetic testing to determine whether that individual is a carrier of a mutant gene that is correlated with certain diseases or conditions.

A PAK inhibitor and the additional therapy(ies) are optionally administered before, during or after the occurrence of a disease or condition, and the timing of administering the composition containing a PAK inhibitor varies in some embodiments. Thus, for example, the PAK inhibitor is used as a prophylactic and administered continuously to individuals with a propensity to develop conditions or diseases in order to prevent the occurrence of the disease or condition. The PAK inhibitors and compositions are optionally administered to an individual during or as soon as possible after the onset of the symptoms. The administration of the compounds are optionally initiated within the first 48 hours of the onset of the symptoms, preferably within the first 48 hours of the onset of the symptoms, more preferably within the first 6 hours of the onset of the symptoms, and most preferably within 3 hours of the onset of the symptoms. The initial administration is optionally via any route practical, such as, for example, an intravenous injection, a bolus injection, infusion over 5 minutes to about 5 hours, a pill, a capsule, transdermal patch, buccal delivery, and the like, or combination thereof. A PAK inhibitor is optionally administered as soon as is practicable after the onset of a disease or condition is detected or suspected, and for a length of time necessary for the treatment of the disease, such as, for example, from about 1 month to about 3 months. The length of treatment optionally varies for each individual, and the length is then determined using the known criteria. For example, the PAK inhibitor or a formulation containing the PAK inhibitor is administered for at least 2 weeks, preferably about 1 month to about 5 years, and more preferably from about 1 month to about 3 years.

In some embodiments, the particular choice of compounds depends upon the diagnosis of the attending physicians and their judgment of the condition of an individual and the appropriate treatment protocol. The compounds are optionally administered concurrently (e.g., simultaneously, essentially simultaneously or within the same treatment protocol) or sequentially, depending upon the nature of the disease, disorder, or condition, the condition of an individual, and the actual choice of compounds used. In certain instances, the determination of the order of administration, and the number of repetitions of administration of each therapeutic agent during a treatment protocol, is based on an evaluation of the disease being treated and the condition of an individual.

In some embodiments, therapeutically-effective dosages vary when the drugs are used in treatment combinations. Methods for experimentally determining therapeutically-effective dosages of drugs and other agents for use in combination treatment regimens are described in the literature.

In some embodiments of the combination therapies described herein, dosages of the co-administered compounds vary depending on the type of co-drug employed, on the specific drug employed, on the disease or condition being treated and so forth. In addition, when co-administered with one or more biologically active agents, the compound provided herein is optionally administered either simultaneously with the biologically active agent(s), or sequentially. In certain instances, if administered sequentially, the attending physician will decide on the appropriate sequence of therapeutic compound described herein in combination with the additional therapeutic agent.

The multiple therapeutic agents (at least one of which is a therapeutic compound described herein) are optionally administered in any order or even simultaneously. If simultaneously, the multiple therapeutic agents are optionally provided in a single, unified form, or in multiple forms (by way of example only, either as a single pill or as two separate pills). In certain instances, one of the therapeutic agents is optionally given in multiple doses. In other instances, both are optionally given as multiple doses. If not simultaneous, the timing between the multiple doses is any suitable timing, e.g, from more than zero weeks to less than four weeks. In some embodiments, the additional therapeutic agent is utilized to achieve reversal or amelioration of symptoms of a CNS disorder, whereupon the therapeutic agent described herein (e.g., a compound of any one of Formula I-XV is subsequently administered. In addition, the combination methods, compositions and formulations are not to be limited to the use of only two agents; the use of multiple therapeutic combinations are also envisioned (including two or more compounds described herein).

In certain embodiments, a dosage regimen to treat, prevent, or ameliorate the condition(s) for which relief is sought, is modified in accordance with a variety of factors. These factors include the disorder from which an individual suffers, as well as the age, weight, sex, diet, and medical condition of an individual. Thus, in various embodiments, the dosage regimen actually employed varies and deviates from the dosage regimens set forth herein.

Examples of Pharmaceutical Compositions and Methods of Administration

Provided herein, in certain embodiments, are compositions comprising a therapeutically effective amount of any compound described herein (e.g., a compound of Formula I-XV.

Pharmaceutical compositions are formulated using one or more physiologically acceptable carriers including excipients and auxiliaries which facilitate processing of the active compounds into preparations which are used pharmaceutically. Proper formulation is dependent upon the route of administration chosen. A summary of pharmaceutical compositions is found, for example, in Remington: The Science and Practice of Pharmacy, Nineteenth Ed (Ea hston, Pa.: Mack Publishing Company, 1995); Hoover, John E., Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, Pa. 1975; Liberman, H. A. and Lachman, L., Eds., Pharmaceutical Dosage Forms, Marcel Decker, New York, N.Y., 1980; and Pharmaceutical Dosage Forms and Drug Delivery Systems, Seventh Ed. (Lippincott Williams & Wilkins, 1999).

Provided herein are pharmaceutical compositions that include one or more PAK inhibitors and a pharmaceutically acceptable diluent(s), excipient(s), or carrier(s). In addition, the PAK inhibitor is optionally administered as pharmaceutical compositions in which it is mixed with other active ingredients, as in combination therapy. In some embodiments, the pharmaceutical compositions includes other medicinal or pharmaceutical agents, carriers, adjuvants, such as preserving, stabilizing, wetting or emulsifying agents, solution promoters, salts for regulating the osmotic pressure, and/or buffers. In addition, the pharmaceutical compositions also contain other therapeutically valuable substances.

A pharmaceutical composition, as used herein, refers to a mixture of a PAK inhibitor with other chemical components, such as carriers, stabilizers, diluents, dispersing agents, suspending agents, thickening agents, and/or excipients. The pharmaceutical composition facilitates administration of the PAK inhibitor to an organism. In practicing the methods of treatment or use provided herein, therapeutically effective amounts of a PAK inhibitor are administered in a pharmaceutical composition to a mammal having a condition, disease, or disorder to be treated. Preferably, the mammal is a human. A therapeutically effective amount varies depending on the severity and stage of the condition, the age and relative health of an individual, the potency of the PAK inhibitor used and other factors. The PAK inhibitor is optionally used singly or in combination with one or more therapeutic agents as components of mixtures.

The pharmaceutical formulations described herein are optionally administered to an individual by multiple administration routes, including but not limited to, oral, parenteral (e.g., intravenous, subcutaneous, intramuscular), intranasal, buccal, topical, rectal, or transdermal administration routes. By way of example only, Example 26a is describes a parenteral formulation, Example 26f describes a rectal formulation. The pharmaceutical formulations described herein include, but are not limited to, aqueous liquid dispersions, self-emulsifying dispersions, solid solutions, liposomal dispersions, aerosols, solid dosage forms, powders, immediate release formulations, controlled release formulations, fast melt formulations, tablets, capsules, pills, delayed release formulations, extended release formulations, pulsatile release formulations, multiparticulate formulations, and mixed immediate and controlled release formulations.

The pharmaceutical compositions will include at least one PAK inhibitor, as an active ingredient in free-acid or free-base form, or in a pharmaceutically acceptable salt form. In addition, the methods and pharmaceutical compositions described herein include the use of N-oxides, crystalline forms (also known as polymorphs), as well as active metabolites of these PAK inhibitors having the same type of activity. In some situations, PAK inhibitors exist as tautomers. All tautomers are included within the scope of the compounds presented herein. Additionally, the PAK inhibitor exists in unsolvated as well as solvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like. The solvated forms of the PAK inhibitors presented herein are also considered to be disclosed herein.

“Carrier materials” include any commonly used excipients in pharmaceutics and should be selected on the basis of compatibility with compounds disclosed herein, such as, a PAK inhibitor, and the release profile properties of the desired dosage form. Exemplary carrier materials include, e.g., binders, suspending agents, disintegration agents, filling agents, surfactants, solubilizers, stabilizers, lubricants, wetting agents, diluents, and the like.

Moreover, the pharmaceutical compositions described herein, which include a PAK inhibitor, are formulated into any suitable dosage form, including but not limited to, aqueous oral dispersions, liquids, gels, syrups, elixirs, slurries, suspensions and the like, for oral ingestion by a patient to be treated, solid oral dosage forms, aerosols, controlled release formulations, fast melt formulations, effervescent formulations, lyophilized formulations, tablets, powders, pills, dragees, capsules, delayed release formulations, extended release formulations, pulsatile release formulations, multiparticulate formulations, and mixed immediate release and controlled release formulations. In some embodiments, a formulation comprising a PAK inhibitor is a solid drug dispersion. A solid dispersion is a dispersion of one or more active ingredients in an inert carrier or matrix at solid state prepared by the melting (or fusion), solvent, or melting-solvent methods. (Chiou and Riegelman, Journal of Pharmaceutical Sciences, 60, 1281 (1971)). The dispersion of one or more active agents in a solid diluent is achieved without mechanical mixing. Solid dispersions are also called solid-state dispersions. In some embodiments, any compound described herein (e.g., a compound of Formula I-XV is formulated as a spray dried dispersion (SDD). An SDD is a single phase amorphous molecular dispersion of a drug in a polymer matrix. It is a solid solution prepared by dissolving the drug and a polymer in a solvent (e.g., acetone, methanol or the like) and spray drying the solution. The solvent rapidly evaporates from droplets which rapidly solidifies the polymer and drug mixture trapping the drug in amorphous form as an amorphous molecular dispersion. In some embodiments, such amorphous dispersions are filled in capsules and/or constituted into oral powders for reconstitution. Solubility of an SDD comprising a drug is higher than the solubility of a crystalline form of a drug or a non-SDD amorphous form of a drug. In some embodiments of the methods described herein, PAK inhibitors are administered as SDDs constituted into appropriate dosage forms described herein.

Pharmaceutical preparations for oral use are optionally obtained by mixing one or more solid excipient with a PAK inhibitor, optionally grinding the resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores. Suitable excipients include, for example, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methylcellulose, microcrystalline cellulose, hydroxypropylmethylcellulose, sodium carboxymethylcellulose; or others such as: polyvinylpyrrolidone (PVP or povidone) or calcium phosphate. If desired, disintegrating agents are added, such as the cross linked croscarmellose sodium, polyvinylpyrrolidone, agar, or alginic acid or a salt thereof such as sodium alginate.

Dragee cores are provided with suitable coatings. For this purpose, concentrated sugar solutions are generally used, which optionally contain gum arabic, talc, polyvinylpyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments are optionally added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses.

In some embodiments, the solid dosage forms disclosed herein are in the form of a tablet, (including a suspension tablet, a fast-melt tablet, a bite-disintegration tablet, a rapid-disintegration tablet, an effervescent tablet, or a caplet), a pill, a powder (including a sterile packaged powder, a dispensable powder, or an effervescent powder) a capsule (including both soft or hard capsules, e.g., capsules made from animal-derived gelatin or plant-derived HPMC, or “sprinkle capsules”), solid dispersion, solid solution, bioerodible dosage form, controlled release formulations, pulsatile release dosage forms, multiparticulate dosage forms, pellets, granules, or an aerosol. By way of example, Example 26b describes a solid dosage formulation that is a capsule. In other embodiments, the pharmaceutical formulation is in the form of a powder. In still other embodiments, the pharmaceutical formulation is in the form of a tablet, including but not limited to, a fast-melt tablet. Additionally, pharmaceutical formulations of a PAK inhibitor are optionally administered as a single capsule or in multiple capsule dosage form. In some embodiments, the pharmaceutical formulation is administered in two, or three, or four, capsules or tablets.

In another aspect, dosage forms include microencapsulated formulations. In some embodiments, one or more other compatible materials are present in the microencapsulation material. Exemplary materials include, but are not limited to, pH modifiers, erosion facilitators, anti-foaming agents, antioxidants, flavoring agents, and carrier materials such as binders, suspending agents, disintegration agents, filling agents, surfactants, solubilizers, stabilizers, lubricants, wetting agents, and diluents.

Exemplary microencapsulation materials useful for delaying the release of the formulations including a PAK inhibitor, include, but are not limited to, hydroxypropyl cellulose ethers (HPC) such as Klucel® or Nisso HPC, low-substituted hydroxypropyl cellulose ethers (L-HPC), hydroxypropyl methyl cellulose ethers (HPMC) such as Seppifilm-LC, Pharmacoat®, Metolose SR, Methocel®-E, Opadry YS, PrimaFlo, Benecel MP824, and Benecel MP843, methylcellulose polymers such as Methocel®-A, hydroxypropylmethylcellulose acetate stearate Aqoat (HF-LS, HF-LG, HF-MS) and Metolose®, Ethylcelluloses (EC) and mixtures thereof such as E461, Ethocel®, Aqualon®-EC, Surelease®, Polyvinyl alcohol (PVA) such as Opadry AMB, hydroxyethylcelluloses such as Natrosol®, carboxymethylcelluloses and salts of carboxymethylcelluloses (CMC) such as Aqualon®-CMC, polyvinyl alcohol and polyethylene glycol co-polymers such as Kollicoat IR®, monoglycerides (Myverol), triglycerides (KLX), polyethylene glycols, modified food starch, acrylic polymers and mixtures of acrylic polymers with cellulose ethers such as Eudragit® EPO, Eudragit® L30D-55, Eudragit® FS 30D Eudragit® L100-55, Eudragit® L100, Eudragit® S100, Eudragit® RD100, Eudragit® E100, Eudragit® L12.5, Eudragit® S12.5, Eudragit® NE30D, and Eudragit® NE 40D, cellulose acetate phthalate, sepifilms such as mixtures of HPMC and stearic acid, cyclodextrins, and mixtures of these materials.

The pharmaceutical solid oral dosage forms including formulations described herein, which include a PAK inhibitor, are optionally further formulated to provide a controlled release of the PAK inhibitor. Controlled release refers to the release of the PAK inhibitor from a dosage form in which it is incorporated according to a desired profile over an extended period of time. Controlled release profiles include, for example, sustained release, prolonged release, pulsatile release, and delayed release profiles. In contrast to immediate release compositions, controlled release compositions allow delivery of an agent to an individual over an extended period of time according to a predetermined profile. Such release rates provide therapeutically effective levels of agent for an extended period of time and thereby provide a longer period of pharmacologic response while minimizing side effects as compared to conventional rapid release dosage forms. Such longer periods of response provide for many inherent benefits that are not achieved with the corresponding short acting, immediate release preparations.

In other embodiments, the formulations described herein, which include a PAK inhibitor, are delivered using a pulsatile dosage form. A pulsatile dosage form is capable of providing one or more immediate release pulses at predetermined time points after a controlled lag time or at specific sites. Pulsatile dosage forms including the formulations described herein, which include a PAK inhibitor, are optionally administered using a variety of pulsatile formulations that include, but are not limited to, those described in U.S. Pat. Nos. 5,011,692, 5,017,381, 5,229,135, and 5,840,329. Other pulsatile release dosage forms suitable for use with the present formulations include, but are not limited to, for example, U.S. Pat. Nos. 4,871,549, 5,260,068, 5,260,069, 5,508,040, 5,567,441 and 5,837,284.

Liquid formulation dosage forms for oral administration are optionally aqueous suspensions selected from the group including, but not limited to, pharmaceutically acceptable aqueous oral dispersions, emulsions, solutions, elixirs, gels, and syrups. See, e.g., Singh et al., Encyclopedia of Pharmaceutical Technology, 2nd Ed., pp. 754-757 (2002). In addition to the PAK inhibitor, the liquid dosage forms optionally include additives, such as: (a) disintegrating agents; (b) dispersing agents; (c) wetting agents; (d) at least one preservative, (e) viscosity enhancing agents, (f) at least one sweetening agent, and (g) at least one flavoring agent. In some embodiments, the aqueous dispersions further includes a crystal-forming inhibitor.

In some embodiments, the pharmaceutical formulations described herein are self-emulsifying drug delivery systems (SEDDS). Emulsions are dispersions of one immiscible phase in another, usually in the form of droplets. Generally, emulsions are created by vigorous mechanical dispersion. SEDDS, as opposed to emulsions or microemulsions, spontaneously form emulsions when added to an excess of water without any external mechanical dispersion or agitation. An advantage of SEDDS is that only gentle mixing is required to distribute the droplets throughout the solution. Additionally, water or the aqueous phase is optionally added just prior to administration, which ensures stability of an unstable or hydrophobic active ingredient. Thus, the SEDDS provides an effective delivery system for oral and parenteral delivery of hydrophobic active ingredients. In some embodiments, SEDDS provides improvements in the bioavailability of hydrophobic active ingredients. Methods of producing self-emulsifying dosage forms include, but are not limited to, for example, U.S. Pat. Nos. 5,858,401, 6,667,048, and 6,960,563.

Suitable intranasal formulations include those described in, for example, U.S. Pat. Nos. 4,476,116, 5,116,817 and 6,391,452. Nasal dosage forms generally contain large amounts of water in addition to the active ingredient. Minor amounts of other ingredients such as pH adjusters, emulsifiers or dispersing agents, preservatives, surfactants, gelling agents, or buffering and other stabilizing and solubilizing agents are optionally present.

For administration by inhalation, the PAK inhibitor is optionally in a form as an aerosol, a mist or a powder. Pharmaceutical compositions described herein are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebuliser, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol, the dosage unit is determined by providing a valve to deliver a metered amount. Capsules and cartridges of, such as, by way of example only, gelatin for use in an inhaler or insufflator are formulated containing a powder mix of the PAK inhibitor and a suitable powder base such as lactose or starch. By way of example, Example 26e describes an inhalation formulation.

Buccal formulations that include a PAK inhibitor include, but are not limited to, U.S. Pat. Nos. 4,229,447, 4,596,795, 4,755,386, and 5,739,136. In addition, the buccal dosage forms described herein optionally further include a bioerodible (hydrolysable) polymeric carrier that also serves to adhere the dosage form to the buccal mucosa. The buccal dosage form is fabricated so as to erode gradually over a predetermined time period, wherein the delivery of the PAK inhibitor, is provided essentially throughout. Buccal drug delivery avoids the disadvantages encountered with oral drug administration, e.g., slow absorption, degradation of the active agent by fluids present in the gastrointestinal tract and/or first-pass inactivation in the liver. The bioerodible (hydrolysable) polymeric carrier generally comprises hydrophilic (water-soluble and water-swellable) polymers that adhere to the wet surface of the buccal mucosa. Examples of polymeric carriers useful herein include acrylic acid polymers and co, e.g., those known as “carbomers” (Carbopol®, which may be obtained from B.F. Goodrich, is one such polymer). Other components also be incorporated into the buccal dosage forms described herein include, but are not limited to, disintegrants, diluents, binders, lubricants, flavoring, colorants, preservatives, and the like. For buccal or sublingual administration, the compositions optionally take the form of tablets, lozenges, or gels formulated in a conventional manner. By way of example, Examples 26c and 26d describe sublingual formulations.

Transdermal formulations of a PAK inhibitor are administered for example by those described in U.S. Pat. Nos. 3,598,122, 3,598,123, 3,710,795, 3,731,683, 3,742,951, 3,814,097, 3,921,636, 3,972,995, 3,993,072, 3,993,073, 3,996,934, 4,031,894, 4,060,084, 4,069,307, 4,077,407, 4,201,211, 4,230,105, 4,292,299, 4,292,303, 5,336,168, 5,665,378, 5,837,280, 5,869,090, 6,923,983, 6,929,801 and 6,946,144. By way of example, Example 26g describes a topical formulation.

The transdermal formulations described herein include at least three components: (1) a formulation of a PAK inhibitor; (2) a penetration enhancer; and (3) an aqueous adjuvant. In addition, transdermal formulations include components such as, but not limited to, gelling agents, creams and ointment bases, and the like. In some embodiments, the transdermal formulation further includes a woven or non-woven backing material to enhance absorption and prevent the removal of the transdermal formulation from the skin. In other embodiments, the transdermal formulations described herein maintain a saturated or supersaturated state to promote diffusion into the skin.

In some embodiments, formulations suitable for transdermal administration of a PAK inhibitor employ transdermal delivery devices and transdermal delivery patches and are lipophilic emulsions or buffered, aqueous solutions, dissolved and/or dispersed in a polymer or an adhesive. Such patches are optionally constructed for continuous, pulsatile, or on demand delivery of pharmaceutical agents. Still further, transdermal delivery of the PAK inhibitor is optionally accomplished by means of iontophoretic patches and the like. Additionally, transdermal patches provide controlled delivery of the PAK inhibitor. The rate of absorption is optionally slowed by using rate-controlling membranes or by trapping the PAK inhibitor within a polymer matrix or gel. Conversely, absorption enhancers are used to increase absorption. An absorption enhancer or carrier includes absorbable pharmaceutically acceptable solvents to assist passage through the skin. For example, transdermal devices are in the form of a bandage comprising a backing member, a reservoir containing the PAK inhibitor optionally with carriers, optionally a rate controlling barrier to deliver the PAK inhibitor to the skin of the host at a controlled and predetermined rate over a prolonged period of time, and means to secure the device to the skin.

Formulations that include a PAK inhibitor suitable for intramuscular, subcutaneous, or intravenous injection include physiologically acceptable sterile aqueous or non-aqueous solutions, dispersions, suspensions or emulsions, and sterile powders for reconstitution into sterile injectable solutions or dispersions. Examples of suitable aqueous and non-aqueous carriers, diluents, solvents, or vehicles including water, ethanol, polyols (propyleneglycol, polyethylene-glycol, glycerol, cremophor and the like), suitable mixtures thereof, vegetable oils (such as olive oil) and injectable organic esters such as ethyl oleate. Proper fluidity is maintained, for example, by the use of a coating such as lecithin, by the maintenance of the required particle size in the case of dispersions, and by the use of surfactants. Formulations suitable for subcutaneous injection also contain optional additives such as preserving, wetting, emulsifying, and dispensing agents.

For intravenous injections, a PAK inhibitor is optionally formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hank's solution, Ringer's solution, or physiological saline buffer. For transmucosal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. For other parenteral injections, appropriate formulations include aqueous or nonaqueous solutions, preferably with physiologically compatible buffers or excipients.

Parenteral injections optionally involve bolus injection or continuous infusion. Formulations for injection are optionally presented in unit dosage form, e.g., in ampoules or in multi dose containers, with an added preservative. In some embodiments, the pharmaceutical composition described herein are in a form suitable for parenteral injection as a sterile suspensions, solutions or emulsions in oily or aqueous vehicles, and contain formulatory agents such as suspending, stabilizing and/or dispersing agents. Pharmaceutical formulations for parenteral administration include aqueous solutions of the PAK inhibitor in water soluble form. Additionally, suspensions of the PAK inhibitor are optionally prepared as appropriate oily injection suspensions.

In some embodiments, the PAK inhibitor is administered topically and formulated into a variety of topically administrable compositions, such as solutions, suspensions, lotions, gels, pastes, medicated sticks, balms, creams or ointments. Such pharmaceutical compositions optionally contain solubilizers, stabilizers, tonicity enhancing agents, buffers and preservatives.

The PAK inhibitor is also optionally formulated in rectal compositions such as enemas, rectal gels, rectal foams, rectal aerosols, suppositories, jelly suppositories, or retention enemas, containing conventional suppository bases such as cocoa butter or other glycerides, as well as synthetic polymers such as polyvinylpyrrolidone, PEG, and the like. In suppository forms of the compositions, a low-melting wax such as, but not limited to, a mixture of fatty acid glycerides, optionally in combination with cocoa butter is first melted.

Examples of Methods of Dosing and Treatment Regimens

The PAK inhibitor is optionally used in the preparation of medicaments for the prophylactic and/or therapeutic treatment of a CNS disorder that would benefit, at least in part, from amelioration of symptoms. In addition, a method for treating any of the diseases or conditions described herein in an individual in need of such treatment, involves administration of pharmaceutical compositions containing at least one PAK inhibitor described herein, or a pharmaceutically acceptable salt, pharmaceutically acceptable N-oxide, pharmaceutically active metabolite, pharmaceutically acceptable prodrug, or pharmaceutically acceptable solvate thereof, in therapeutically effective amounts to said individual.

In the case wherein the patient's condition does not improve, upon the doctor's discretion the administration of the PAK inhibitor is optionally administered chronically, that is, for an extended period of time, including throughout the duration of the patient's life in order to ameliorate or otherwise control or limit the symptoms of the patient's disease or condition.

In the case wherein the patient's status does improve, upon the doctor's discretion the administration of the PAK inhibitor is optionally given continuously; alternatively, the dose of drug being administered is temporarily reduced or temporarily suspended for a certain length of time (i.e., a “drug holiday”). The length of the drug holiday optionally varies between 2 days and 1 year, including by way of example only, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, 10 days, 12 days, 15 days, 20 days, 28 days, 35 days, 50 days, 70 days, 100 days, 120 days, 150 days, 180 days, 200 days, 250 days, 280 days, 300 days, 320 days, 350 days, or 365 days. The dose reduction during a drug holiday includes from 10%-100%, including, by way of example only, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, or 100%.

Once improvement of the patient's conditions has occurred, a maintenance dose is administered if necessary. Subsequently, the dosage or the frequency of administration, or both, is reduced, as a function of the symptoms, to a level at which the improved disease, disorder or condition is retained. In some embodiments, patients require intermittent treatment on a long-term basis upon any recurrence of symptoms.

In some embodiments, the pharmaceutical compositions described herein are in unit dosage forms suitable for single administration of precise dosages. In unit dosage form, the formulation is divided into unit doses containing appropriate quantities of one or more PAK inhibitor. In some embodiments, the unit dosage is in the form of a package containing discrete quantities of the formulation. Non-limiting examples are packaged tablets or capsules, and powders in vials or ampoules. In some embodiments, aqueous suspension compositions are packaged in single-dose non-reclosable containers. Alternatively, multiple-dose reclosable containers are used, in which case it is typical to include a preservative in the composition. By way of example only, formulations for parenteral injection are presented in unit dosage form, which include, but are not limited to ampoules, or in multi dose containers, with an added preservative.

The daily dosages appropriate for the PAK inhibitor are from about 0.01 to about 2.5 mg/kg per body weight. An indicated daily dosage in the larger mammal, including, but not limited to, humans, is in the range from about 0.5 mg to about 1000 mg, conveniently administered in divided doses, including, but not limited to, up to four times a day or in extended release form. Suitable unit dosage forms for oral administration include from about 1 to about 500 mg active ingredient, from about 1 to about 250 mg of active ingredient, or from about 1 to about 100 mg active ingredient. The foregoing ranges are merely suggestive, as the number of variables in regard to an individual treatment regime is large, and considerable excursions from these recommended values are not uncommon. Such dosages are optionally altered depending on a number of variables, not limited to the activity of the PAK inhibitor used, the disease or condition to be treated, the mode of administration, the requirements of an individual, the severity of the disease or condition being treated, and the judgment of the practitioner.

Toxicity and therapeutic efficacy of such therapeutic regimens are optionally determined in cell cultures or experimental animals, including, but not limited to, the determination of the LD50 (the dose lethal to 50% of the population) and the ED50 (the dose therapeutically effective in 50% of the population). The dose ratio between the toxic and therapeutic effects is the therapeutic index, which is expressed as the ratio between LD50 and ED50. PAK inhibitors exhibiting high therapeutic indices are preferred. The data obtained from cell culture assays and animal studies is optionally used in formulating a range of dosage for use in human. The dosage of such PAK inhibitors lies preferably within a range of circulating concentrations that include the ED50 with minimal toxicity. The dosage optionally varies within this range depending upon the dosage form employed and the route of administration utilized.

Assays for Identification and Characterization of PAK Inhibitors

Small molecule PAK inhibitors are optionally identified in high-throughput in vitro or cellular assays as described in, e.g., Yu et al (2001), J Biochem (Tokyo); 129(2):243-251; Rininsland et al (2005), BMC Biotechnol, 5:16; and Allen et al (2006), ACS Chem Biol; 1(6):371-376. PAK inhibitors suitable for the methods described herein are available from a variety of sources including both natural (e.g., plant extracts) and synthetic. For example, candidate PAK inhibitors are isolated from a combinatorial library, i.e., a collection of diverse chemical compounds generated by either chemical synthesis or biological synthesis by combining a number of chemical “building blocks.” For example, a linear combinatorial chemical library such as a polypeptide library is formed by combining a set of chemical building blocks called amino acids in every possible way for a given compound length (i.e., the number of amino acids in a polypeptide compound). Millions of chemical compounds can be synthesized through such combinatorial mixing of chemical building blocks, as desired. Theoretically, the systematic, combinatorial mixing of 100 interchangeable chemical building blocks results in the synthesis of 100 million tetrameric compounds or 10 billion pentameric compounds. See Gallop et al. (1994), J. Med. Chem. 37(9), 1233. Each member of a library may be singular and/or may be part of a mixture (e.g. a “compressed library”). The library may comprise purified compounds and/or may be “dirty” (i.e., containing a quantity of impurities). Preparation and screening of combinatorial chemical libraries are documented methodologies. See Cabilly, ed., Methods in Molecular Biology, Humana Press, Totowa, N.J., (1998). Combinatorial chemical libraries include, but are not limited to: diversomers such as hydantoins, benzodiazepines, and dipeptides, as described in, e.g., Hobbs et al. (1993), Proc. Natl. Acad. Sci. U.S.A. 90, 6909; analogous organic syntheses of small compound libraries, as described in Chen et al. (1994), J. Amer. Chem. Soc., 116: 2661; Oligocarbamates, as described in Cho, et al. (1993), Science 261, 1303; peptidyl phosphonates, as described in Campbell et al. (1994), J. Org. Chem., 59: 658; and small organic molecule libraries containing, e.g., thiazolidinones and metathiazanones (U.S. Pat. No. 5,549,974), pyrrolidines (U.S. Pat. Nos. 5,525,735 and 5,519,134), benzodiazepines (U.S. Pat. No. 5,288,514). In addition, numerous combinatorial libraries are commercially available from, e.g., ComGenex (Princeton, N.J.); Asinex (Moscow, Russia); Tripos, Inc. (St. Louis, Mo.); ChemStar, Ltd. (Moscow, Russia); 3D Pharmaceuticals (Exton, Pa.); and Martek Biosciences (Columbia, Md.).

Devices for the preparation of combinatorial libraries are commercially available (see, e.g., 357 MPS, 390 MPS from Advanced Chem Tech, Louisville, Ky.; Symphony from Rainin, Woburn, Mass.; 433A from Applied Biosystems, Foster City, Calif.; and 9050 Plus from Millipore, Bedford, Mass.). A number of robotic systems have also been developed for solution phase chemistries. These systems include automated workstations like the automated synthesis apparatus developed by Takeda Chemical Industries, LTD (Osaka, Japan), and many robotic systems utilizing robotic arms (Zymate II). Any of the above devices are optionally used to generate combinatorial libraries for identification and characterization of PAK inhibitors which mimic the manual synthetic operations performed by small molecule PAK inhibitors suitable for the methods described herein. Any of the above devices are optionally used to identify and characterize small molecule PAK inhibitors suitable for the methods disclosed herein. In many of the embodiments disclosed herein, PAK inhibitors, PAK binding molecules, and PAK clearance agents are disclosed as polypeptides or proteins (where polypeptides comprise two or more amino acids). In these embodiments, the inventors also contemplate that PAK inhibitors, binding molecules, and clearance agents also include peptide mimetics based on the polypeptides, in which the peptide mimetics interact with PAK or its upstream or downstream regulators by replicating the binding or substrate interaction properties of PAK or its regulators. Nucleic acid aptamers are also contemplated as PAK inhibitors, binding molecules, and clearance agents, as are small molecules other than peptides or nucleic acids. For example, in some embodiments small molecule PAK binding partners, inhibitors, or clearance agents, or small molecule agonists or antagonists of PAK modulators or targets, are designed or selected based on analysis of the structure of PAK or its modulators or targets and binding interactions with interacting molecules, using “rational drug design” (see, for example Jacobsen et al. (2004) Molecular Interventions 4:337-347; Shi et al. (2007) Bioorg. Med. Chem. Lett. 17:6744-6749).

The identification of potential PAK inhibitors is determined by, for example, assaying the in vitro kinase activity of PAK in the presence of candidate inhibitors. In such assays, PAK and/or a characteristic PAK fragment produced by recombinant means is contacted with a substrate in the presence of a phosphate donor (e.g., ATP) containing radiolabeled phosphate, and PAK-dependent incorporation is measured. “Substrate” includes any substance containing a suitable hydroxyl moiety that can accept the γ-phosphate group from a donor molecule such as ATP in a reaction catalyzed by PAK. The substrate may be an endogenous substrate of PAK, i.e. a naturally occurring substance that is phosphorylated in unmodified cells by naturally-occurring PAK or any other substance that is not normally phosphorylated by PAK in physiological conditions, but may be phosphorylated in the employed conditions. The substrate may be a protein or a peptide, and the phosphrylation reaction may occur on a serine and/or threonine residue of the substrate. For example, specific substrates, which are commonly employed in such assays include, but are not limited to, histone proteins and myelin basic protein. In some embodiments, PAK inhibitors are identified using IMAP® technology.

Detection of PAK dependent phosphorylation of a substrate can be quantified by a number of means other than measurement of radiolabeled phosphate incorporation. For example, incorporation of phosphate groups may affect physiochemical properties of the substrate such as electrophoretic mobility, chromatographic properties, light absorbance, fluorescence, and phosphorescence. Alternatively, monoclonal or polyclonal antibodies can be generated which selectively recognize phosphorylated forms of the substrate from non-phosphorylated forms whereby allowing antibodies to function as an indicator of PAK kinase activity.

High-throughput PAK kinase assays can be performed in, for example, microtiter plates with each well containing PAK kinase or an active fragment thereof, substrate covalently linked to each well, P³² radiolabeled ATP and a potential PAK inhibitor candidate. Microtiter plates can contain 96 wells or 1536 wells for large scale screening of combinatorial library compounds. After the phosphorylation reaction has completed, the plates are washed leaving the bound substrate. The plates are then detected for phosphate group incorporation via autoradiography or antibody detection. Candidate PAK inhibitors are identified by their ability to decease the amount of PAK phosphotransferase ability upon a substrate in comparison with PAK phosphotransferase ability alone.

The identification of potential PAK inhibitors may also be determined, for example, via in vitro competitive binding assays on the catalytic sites of PAK such as the ATP binding site and/or the substrate binding site. For binding assays on the ATP binding site, a known protein kinase inhibitor with high affinity to the ATP binding site is used such as staurosporine. Staurosporine is immobilized and may be fluorescently labeled, radiolabeled or in any manner that allows detection. The labeled staurosporine is introduced to recombinantly expressed PAK protein or a fragment thereof along with potential PAK inhibitor candidates. The candidate is tested for its ability to compete, in a concentration-dependant manner, with the immobilized staurosporine for binding to the PAK protein. The amount of staurosporine bound PAK is inversely proportional to the affinity of the candidate inhibitor for PAK. Potential inhibitors would decrease the quantifiable binding of staurosporine to PAK. See e.g., Fabian et al (2005) Nat. Biotech., 23:329. Candidates identified from this competitive binding assay for the ATP binding site for PAK would then be further screened for selectivity against other kinases for PAK specificity.

The identification of potential PAK inhibitors may also be determined, for example, by in cyto assays of PAK activity in the presence of the inhibitor candidate. Various cell lines and tissues may be used, including cells specifically engineered for this purpose. In cyto screening of inhibitor candidates may assay PAK activity by monitoring the downstream effects of PAK activity. Such effects include, but are not limited to, the formation of peripheral actin microspikes and or associated loss of stress fibers as well as other cellular responses such as growth, growth arrest, differentiation, or apoptosis. See e.g., Zhao et al., (1998) Mol. Cell. Biol. 18:2153. For example in a PAK yeast assay, yeast cells grow normally in glucose medium. Upon exposure to galactose however, intracellular PAK expression is induced, and in turn, the yeast cells die. Candidate compounds that inhibit PAK activity are identified by their ability to prevent the yeast cells from dying from PAK activation.

Alternatively, PAK-mediated phosphorylation of a downstream target of PAK can be observed in cell based assays by first treating various cell lines or tissues with PAK inhibitor candidates followed by lysis of the cells and detection of PAK mediated events. Cell lines used in this experiment may include cells specifically engineered for this purpose. PAK mediated events include, but are not limited to, PAK mediated phosphorylation of downstream PAK mediators. For example, phosphorylation of downstream PAK mediators can be detected using antibodies that specifically recognize the phosphorylated PAK mediator but not the unphosphorylated form. These antibodies have been described in the literature and have been extensively used in kinase screening campaigns. In some instances a phospho LIMK antibody is used after treatment of HeLa cells stimulated with EGF or sphingosine to detect downstream PAK signaling events.

The identification of potential PAK inhibitors may also be determined, for example, by in vivo assays involving the use of animal models, including transgenic animals that have been engineered to have specific defects or carry markers that can be used to measure the ability of a candidate substance to reach and/or affect different cells within the organism. For example, DISC1 knockout mice have defects in synaptic plasticity and behavior from increased numbers of dendritic spines and an abundance of long and immature spines. Thus, identification of PAK inhibitors can comprise administering a candidate to DISC1 knockout mice and observing for reversals in synaptic plasticity and behavior defects as a readout for PAK inhibition.

For example, fragile X mental retardation 1 (FMR1) knockout mice have defects in synaptic plasticity and behavior from increased numbers of dendritic spines and an abundance of long and immature spines. See e.g., Comery et al., (1997) Proc. Natl. Acad. Sci. USA, 94:5401-04. As PAK is a downstream effector of the FMR1 gene, the defects are reversed upon the use of dominant negative transgenes of PAK that inhibit endogenous PAK activity. See Hayashi et al. (2007) Proc. Natl. Acad. Sci. USA, 104:11489-94. Thus, identification of PAK inhibitors can comprise administering a candidate to FMR1 knockout mice and observing for reversals in synaptic plasticity and behavior defects as a readout for PAK inhibition.

For example, suitable animal models for Alzheimer's disease are knock-ins or transgenes of the human mutated genes including transgenes of the “swedish” mutation of APP (APPswe), transgenes expressing the mutant form of presenilin 1 and presenilin 2 found in familial/early onset AD. Thus, identification of PAK inhibitors can comprise administering a candidate to a knock-in animal and observing for reversals in synaptic plasticity and behavior defects as a readout for PAK inhibition.

Administration of the candidate to the animal is via any clinical or non-clinical route, including but not limited to oral, nasal, buccal and/or topical administrations. Additionally or alternatively, administration may be intratracheal instillation, bronchial instillation, intradermal, subcutaneous, intramuscular, intraperitoneal, inhalation, and/or intravenous injection.

Changes in spine morphology are detected using any suitable method, e.g., by use of 3D and/or 4D real time interactive imaging and visualization. In some instances, the Imaris suite of products (available from Bitplane Scientific Solutions) provides functionality for visualization, segmentation and interpretation of 3D and 4D microscopy datasets obtained from confocal and wide field microscopy data.

EXAMPLES

The following specific examples are to be construed as merely illustrative, and not limitative of the remainder of the disclosure in any way whatsoever.

All synthetic chemistry was performed in standard laboratory glassware unless indicated otherwise in the examples. Commercial reagents were used as received. Analytical LC/MS was performed on an Agilent 1200 system with a variable wavelength detector and Agilent 6140 Single quadrupole mass spectrometer, alternating positive and negative ion scans. Retention times were determined from the extracted 220 nm chromatogram. ¹H NMR was performed on a Bruker DRX-400 at 400 MHz. Microwave reactions were performed in a Biotage Initiator using the instrument software to control heating time and pressure. Hydrogenation reactions were performed on a H-Cube using the commercially available catalyst cartridges unless otherwise specified. Silica gel chromatography was performed manually.

Preparative HPLC was performed on a Waters 1525/2487 with UV detection at 220 nm and manual collection.

Analytical LC/MS Method A:

HPLC column: Zorbax SB-C18, 3.5 μm, 2.1 mm×30 mm, maintained at 40° C. HPLC Gradient: 0.4 mL/min, 95:5:0.1 water:acetonitrile:formic acid for 0.1 min then to 5:95:0.1 water:acetonitrile:formic acid in 3.9 min, maintaining for 0.5 min.

Analytical LC/MS Method B:

HPLC column: Kinetex, 2.6 μm, C18, 50×2.1 mm, maintained at 40° C.

HPLC Gradient: 1.0 mL/min, 95:5:0.1 water:acetonitrile:formic acid to 5:95:0.1 water:acetonitrile:formic acid in 2.5 min, maintaining for 0.5 min.

Analytical LC/MS method C was performed on a Shimadzu system with an attached API 165 single quadrupole mass spectrometer. Retention times were determined from the 220 nm chromatogram.

HPLC column: Phenomenex, C18, 2.5 μm, 20×2 mm, maintained at 25° C. HPLC Gradient: 0.5 mL/min, 95:5:0.02 water:acetonitrile:CF₃COOH to 5:95:0.02 water:acetonitrile:CF₃COOH in 2.9 min, maintaining for 0.9 min.

Analytical LC/MS method D was performed on an Agilent 1200 system with a variable wavelength detector and Agilent 6110 Single quadrupole mass spectrometer, positive or negative ion scans (AS/F). Retention times were determined from the 220 nm chromatogram.

Analytical LC/MS method E was performed on an Agilent 1100 system with a variable wavelength detector and Agilent G1946A Single quadrupole mass spectrometer, positive or negative ion scans (AX). Retention times were determined from the 220 nm chromatogram.

Analytical LC/MS method F was performed on an Agilent 1100 system with a variable wavelength detector and Agilent G1946A Single quadrupole mass spectrometer, positive or negative ion scans (I/E/W). Retention times were determined from the 220 nm chromatogram.

Analytical LC/MS method G was performed on an Agilent 1200 system with a variable wavelength detector and Agilent 6110 Single quadrupole mass spectrometer, alternating positive and negative ion scans (AN/B). Retention times were determined from the 220 nm chromatogram.

Analytical LC/MS method H was performed on an Agilent 1200 system with a variable wavelength detector and Agilent G1956A Single quadrupole mass spectrometer, positive or negative ion scans (N). Retention times were determined from the 220 nm chromatogram.

Analytical LC/MS method J was performed on an Agilent 1100 system with a variable wavelength detector and Agilent G1946D Single quadrupole mass spectrometer, positive or negative ion scans (AY). Retention times were determined from the 220 nm chromatogram.

Preparative HPLC method A: Preparative HPLC was performed on a Waters 1525/2487 with UV detection at 220 nm and manual collection.

HPLC column: Zorbax SB-C18 21.2×100 mm.

HPLC Gradient: 20 mL/min, 95:5:0.1 water:methanol:formic acid to 5:95:0.1 water:methanol:formic acid; the gradient shape was optimized for individual separations.

Preparative HPLC Method B:

HPLC column: Reprosil-Pur C18-AQ 250×20 mm.

HPLC Gradient: 25 mL/min, 25:75:0.02 acetonitrile:water:trifluoroacetic acid to 100:0:0.02 acetonitrile:water:trifluoroacetic acid; the gradient shape was optimized for individual separations.

Example 1 Synthesis of 6-(2-chloro-4-[1,3,4]oxadiazol-2-yl-phenyl)-8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-8H-pyrido[2,3-d]pyrimidin-7-one (8) Preparation of Intermediate Compounds Intermediate 1: Synthesis of 6-bromo-8-ethyl-2-(methylthio)pyrido[2,3-d]pyrimidin-7(8H)-one (3)

Step 1: Synthesis of 6-bromo-2-(methylthio)pyrido[2,3-d]pyrimidin-7(8H)-one (2)

To a solution of 2-(methylthio)pyrido[2,3-d]pyrimidin-7(8H)-one (1, 1.00 g, 5.18 mmol) in anhydrous dimethylformamide (25 mL) was added N-bromosuccinimide (0.99 g, 5.59 mmol) portionwise at room temperature, and the reaction mixture was stirred for 18 h. The mixture was concentrated, and the solid was triturated with hot water (1×20 mL), filtered, and washed with isopropanol to give title compound as a pale yellow solid (0.68 g, 2.50 mmol, 48%). ESMS m/z 272 (M+H)⁺; ¹H NMR (400 MHz, DMSO-d₆) δ ppm 12.88 (br. s., 1H), 8.84 (s, 1H), 8.47 (s, 1H), 2.57 (s, 3H).

Step 2: Synthesis of 6-bromo-8-ethyl-2-(methylthio)pyrido[2,3-d]pyrimidin-7(8H)-one (3)

To a suspension of NaH (60%, 0.15 g, 3.75 mmol) in anhydrous dimethylformamide (10 mL) was added 6-bromo-2-(methylthio)pyrido[2,3-d]pyrimidin-7(8H)-one (2, 0.68 g, 2.50 mmol) at room temperature and the reaction was stirred at 50° C. for 0.5 h. The reaction mixture was cooled to room temperature, ethyl bromide (0.22 mL, 0.32 g, 2.93 mmol) was added, and the reaction was stirred at 50° C. for 1.5 h. The mixture was poured into ice water (10 g), and the white precipitate was collected to give 6-bromo-8-ethyl-2-(methylthio)pyrido[2,3-d]pyrimidin-7(8H)-one (3, 0.57 g, 1.90 mmol, 76%). ESMS m/z 300 (M+H)⁺. The material was used without any further purification.

Synthesis of 6-(2-chloro-4-[1,3,4]oxadiazol-2-yl-phenyl)-8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-8H-pyrido[2,3-d]pyrimidin-7-one (8)

Step 3: Synthesis of 6-bromo-8-ethyl-2-methanesulfinyl-8H-pyrido[2,3-d]pyrimidin-7-one (4)

To a solution of 6-bromo-8-ethyl-2-methylsulfanyl-8H-pyrido[2,3-d]pyrimidin-7-one (3, 0.96 g, 3.19 mmol) in dichloromethane (40 mL) was added 3-chloroperbenzoic acid (77%, 0.68 g, 3.04 mmol) in dichloromethane (10 mL) at 0-5° C. and the mixture was stirred at 0-5° C. for 1 h. The reaction mixture was washed with 10% sodium bicarbonate solution (1×20 mL) and water (1×20 mL). The organic layer was dried over sodium sulfate, filtered and evaporated. The title compound was obtained as a pale yellow solid (0.98 g, 3.10 mmol, 97%). ESMS m/z 316 (M+H)⁺.

Step 4: Synthesis of 6-bromo-8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-8H-pyrido[2,3-d]pyrimidin-7-one (5)

6-Bromo-8-ethyl-2-methanesulfinyl-8H-pyrido[2,3-d]pyrimidin-7-one (4, 600 mg, 1.90 mmol) and 4-(4-methylpiperazino)aniline (363 mg, 1.90 mmol) were stirred at 120° C. for 3 h. The reaction mixture was purified by column chromatography using dichloromethane:methanol (100:3→100:5) to give the title compound (340 mg, 0.77 mmol, 40%) as a yellow solid. ESMS m/z 443 (M+H)⁺; ¹H NMR (400 MHz, CDCl₃) δ ppm 8.47 (s, 1H) 7.92 (s, 1H) 7.51 (d, J=8.8 Hz, 2H) 7.24 (br. s., 1H) 6.96 (d, J=8.8 Hz, 2H) 4.48 (q, J=7.0 Hz, 2H) 3.13-3.29 (m, 4H) 2.53-2.64 (m, 4H) 2.36 (s, 3H) 1.35 (t, J=7.0 Hz, 3H).

Step 5: Synthesis of 3-chloro-4-{8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl}benzoic acid methyl ester (6)

6-Bromo-8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-8H-pyrido[2,3-d]pyrimidin-7-one (5, 110 mg, 0.25 mmol), 2-chloro-4-(methoxycarbonyl)benzene boronic acid (58 mg, 0.27 mmol), K₃PO₄ (58 mg, 0.27 mmol) and PdCl₂(dppf) (20 mg, 0.02 mmol) were mixed under argon in a degassed mixture of dimethylformamide and water (20:1, 4.5 mL). The resulting suspension was irradiated for 30 min at 140° C. in a microwave reactor. The reaction mixture was evaporated and the residue was purified by column chromatography, eluting with dichloromethane:methanol (95:5). The title compound (78 mg, 0.15 mmol, 60%) was obtained as a yellow solid. ESMS m/z 533 (M+H)⁺; ¹H NMR (400 MHz, CDCl₃) δ ppm 8.55 (s, 1H) 8.15 (d, J=1.5 Hz, 1H) 7.97 (dd, J=7.9, 1.5 Hz, 1H) 7.51-7.64 (m, 3H) 7.48 (d, J=7.8 Hz, 1H) 7.27 (br. s., 1H) 6.97 (d, J=9.0 Hz, 2H) 4.49 (q, J=7.3 Hz, 2H) 3.95 (s, 3H) 3.18-3.35 (m, 4H) 2.67 (br. s., 4H) 2.42 (br. s., 3H) 1.38 (t, J=7.3 Hz, 3H).

Step 6: Synthesis of 3-chloro-4-{8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-7-oxo-7,8-dihydro-pyrido[2,3-d]pyrimidin-6-yl}-benzoic acid hydrazide (7)

3-Chloro-4-{8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-7-oxo-7,8-dihydro-pyrido[2,3-d]pyrimidin-6-yl}benzoic acid methyl ester (6, 77 mg, 0.14 mmol) in the mixture of ethanol (4 mL) and hydrazine hydrate (1 mL) was heated at reflux for 2 h. The reaction mixture was cooled and the yellow precipitate was collected and washed with 2-propanol and diethyl ether to afford the title compound (40 mg, 0.08 mmol, 57%) as a yellow solid. ESMS m/z 533 (M+H)⁺; ¹H NMR (400 MHz, DMSO-d₆) δ ppm 9.94 (br. s., 2H) 8.77 (s, 1H) 7.95 (d, J=1.5 Hz, 1H) 7.87 (s, 1H) 7.83 (dd, J=7.8, 1.5 Hz, 1H) 7.66 (d, J=9.0 Hz, 2H) 7.51 (d, J=7.8 Hz, 1H) 6.94 (d, J=9.0 Hz, 2H) 4.56 (br. s., 2H) 4.36 (q, J=7.0 Hz, 2H) 3.05-3.15 (m, 4H) 2.42-2.48 (m, 4H) 2.22 (s, 3H) 1.28 (t, J=7.0 Hz, 3H).

Step 7: Synthesis of 6-(2-chloro-4-[1,3,4]oxadiazol-2-yl-phenyl)-8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-8H-pyrido[2,3-d]pyrimidin-7-one (8)

3-Chloro-4-{8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-7-oxo-7,8-dihydro-pyrido[2,3-d]pyrimidin-6-yl}benzoic acid hydrazide (7, 30 mg, 0.06 mmol) was suspended in triethyl orthoformate (5 mL) to which was added trifluoroacetic acid (1 mL). The resulting reaction mixture was heated at 130° C. for 2 h. The volatiles were removed and the residue was taken up in dichloromethane (1×20 mL) and washed with 10% sodium hydroxide solution (2×10 mL). The organic layer was dried over sodium sulfate, filtered and evaporated. The residue was purified by column chromatography using dichloromethane:methanol (95:5). The title compound (18 mg, 0.03 mmol, 50%) was obtained as a yellow solid. ESMS m/z 543 (M+H)⁺; ¹H NMR (400 MHz, CDCl₃) δ ppm 8.57 (s, 1H) 8.50 (s, 1H) 8.21 (d, J=1.5 Hz, 1H) 8.04 (dd, J=8.0, 1.5 Hz, 1H) 7.62 (s, 1H) 7.52-7.60 (m, 3H) 7.29 (br. s., 1H) 6.98 (d, J=9.0 Hz, 2H) 4.50 (q, J=6.8 Hz, 2H) 3.16-3.27 (m, 4H) 2.56-2.65 (m, 4H) 2.37 (s, 3H) 1.39 (d, J=6.8 Hz, 3H).

Example 2 Synthesis of 6-[2-chloro-4-(thiophen-2-yl)phenyl]-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (13)

Preparation of Intermediate Compounds Intermediate 2: Synthesis of ethyl 4-bromo-2-chlorophenylacetate (19)

Step 1: Synthesis of (4-bromo-2-chlorophenyl)methanol (15)

4-Bromo-2-chlorobenzoic acid (14, 92.0 g, 0.39 mol) was dissolved in dry tetrahydrofuran (920 mL) and cooled to −15° C. Isobutyryl choroformate (51.0 mL, 0.39 mol) was added followed by N-methylmorpholine (43.5 mL, 0.39 mol). The resulting mixture was stirred for 10 minutes at −15° C., cooled to −25° C. and the precipitated N-methylmorpholine hydrochloride salt was filtered off. The filtrate was warmed to −5° C. and a solution of sodium borohydride (22.19 g, 0.586 mol) in water (190 mL) was added dropwise to the mixture keeping the temperature below 0° C. After stirring for 1 h at 0° C., the volatiles were evaporated, and the residue was diluted with water (500 mL) and dichloromethane (450 mL). The layers were separated and the aqueous layer was extracted with dichloromethane (150 mL). The combined organic layers were washed with water (150 mL), dried over sodium sulfate and evaporated. The product (86.1 g, 0.39 mol, 99%) was obtained as a white crystalline solid.

Step 2: Synthesis of 4-bromo-1-bromomethyl-2-chlorobenzene (16)

Phosphorus tribromide (40.5 mL, 0.431 mol) was added dropwise to a solution of (4-bromo-2-chlorophenyl)-methanol (15, 86.1 g, 0.386 mol) in dichloroethane (430 mL) at 0° C. The reaction mixture was stirred for 10 minutes at this temperature then for 0.5 h at 10° C. The mixture was cooled to 0° C. and a sodium hydroxide solution (600 mL, 2N) was added dropwise. The two layers were separated and the aqueous layer was extracted with dichloroethane (200 mL). The combined organic layers were washed with water (200 mL), dried over sodium sulfate and evaporated in vacuo. The crude product (91 g) was distilled under reduced pressure (7 mmHg), to give 4-bromo-1-bromomethyl-2-chlorobenzene (62.5 g, 0.22 mol, 57%) as a colorless oil.

Step 3: Synthesis of (4-bromo-2-chlorophenyl)acetonitrile (17)

To a stirred solution of 4-bromo-1-bromomethyl-2-chlorobenzene (16, 62.5 g, 0.22 mol) in dichloroethane (522 mL) and water (480 mL) was added tetrabutylammonium chloride (5.05 g), followed by a solution of potassium cyanide (43.2 g, 75.8 mmol) in water (523 mL). The solution was stirred for 4 h at room temperature. The layers were separated and the aqueous layer was extracted with dichloroethane (100 mL). The combined organic layers were washed with water (100 mL), dried over sodium sulfate filtered and evaporated. The crude product (52 g) was distilled under reduced pressure (1 mmHg), affording (4-bromo-2-chlorophenyl)-acetonitrile (45.5 g, 0.220 mol, 90%).

Step 4: Synthesis of (4-bromo-2-chlorophenyl)acetic acid (18)

(4-Bromo-2-chlorophenyl)acetonitrile (17, 45.5 g, 0.22 mol) was added to 675 mL sodium hydroxide solution (8.2%) and heated at reflux for 4 h. The homogeneous solution was cooled to room temperature and concentrated hydrochloric acid (117 mL) was added. The mixture was extracted with dichloromethane (500, 200 mL). The combined organic layers were washed with water (100 mL), dried over sodium sulfate and filtered. The filtrate was treated with charcoal (4.5 g), filtered and evaporated. The residue was triturated with hexane (200 mL) and the solid was collected to give (4-bromo-2-chlorophenyl)-acetic acid (44.6 g, 0.18 mol, 81%) as a white crystalline solid. ESMS m/z 497 [2M−H]⁻.

Step 5: Synthesis of (4-bromo-2-chlorophenyl)acetic acid ethyl ester (19)

(4-Bromo-2-chlorophenyl)-acetic acid (18, 44.03 g, 0.18 mol) was dissolved in methanol (440 mL) and thionyl chloride (44.0 mL, 0.61 mol) was added dropwise. The mixture was refluxed for 1 h, and evaporated. The residue was taken up in toluene and evaporated (2×100 mL). The crude oily product was dissolved in dichloromethane (300 mL) and washed with water (2×100 mL), and the organic solution was dried over sodium sulfate, filtered and evaporated. The residue was dried in high vacuum (0.2 mmHg) at room temperature to give the title compound, solidifying to a light yellow low melting crystalline solid (45.5 g, 0.16 mol, 93%) ESMS m/z 294 [M+H+NH₃]⁺; ¹H NMR (300 MHz, CDCl₃): δ 7.55 (1H, d, J=1.8 Hz), 7.37 (1H, dd, J=8.2, 1.8 Hz), 7.16 (1H, d, J=8.2 Hz), 4.18 (2H, q), 3.71 (2H, s), 1.26 (3H, t).

Synthesis of 6-[2-chloro-4-(thiophen-2-yl)phenyl]-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (13)

Step 1: Synthesis of 6-(4-bromo-2-chlorophenyl)-8-ethyl-2-(methylthio)pyrido[2,3-d]pyrimidin-7(8H)-one (10)

To a solution of 4-ethylamino-2-(methylthio)pyrimidine-5-carbaldehyde (9, 1.00 g, 5.07 mmol) in anhydrous dimethylacetamide (10 mL) was added ethyl 4-bromo-2-chlorophenylacetate (1.70 g, 6.12 mmol) and cesium carbonate (3.30 g, 10.13 mmol). The reaction mixture was stirred at 100° C. for 2 h. The mixture was poured into ice water and the orange solid was collected, washed with water, dried and purified by Teledyne-Isco using a hexane:ethyl acetate gradient (1:0→4:1) to afford the title compound as a white solid (0.30 g, 0.73 mmol, 14%). ESMS m/z 410 (M+H)⁺; ¹H NMR (400 MHz, CDCl₃) δ ppm 8.65 (s, 1H), 7.66 (d, J=1.5 Hz, 1H), 7.63 (s, 1H), 7.47 (dd, J=8.3, 1.5 Hz, 1H), 7.26 (d, J=8.31 Hz, 1H), 4.55 (q, J=7.0 Hz, 2H), 2.66 (s, 3H), 1.37 (t, J=7.0 Hz, 3H).

Step 2: Synthesis of 6-(4-bromo-2-chlorophenyl)-8-ethyl-2-(methylsulfinyl)pyrido[2,3-d]pyrimidin-7(8H)-one (11)

To a solution of 6-(4-bromo-2-chlorophenyl)-8-ethyl-2-(methylthio)-pyrido[2,3-d]pyrimidin-7(8H)-one (10, 1.30 g, 3.16 mmol) in dichloromethane (20 mL) was added dropwise a solution of 3-chloroperbenzoic acid (77%, 0.57 g, 2.54 mmol) in dichloromethane (5 mL) at 0-5° C. and the mixture was stirred for 5 h. The reaction mixture was washed with saturated sodium bicarbonate solution (2×20 mL) and water (10 mL), and the organic layer was dried over sodium sulfate, filtered and evaporated. The crude product was purified by silica gel column chromatography using dichloromethane:ethyl acetate (5:1→5:2→2:1→1:1) to give the title compound as an off-white solid (0.96 g, 2.25 mmol, 71%). ESMS m/z 426 (M+H)⁺.

Step 3: Synthesis of 6-(4-bromo-2-chlorophenyl)-8-ethyl-2-(4-(4-methylpiperazin-1-yl)-phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (12)

6-(4-Bromo-2-chlorophenyl)-8-ethyl-2-(methylsulfinyl)pyrido[2,3-d]pyrimidin-7(8H)-one (11, 0.60 g, 1.41 mmol) and 4-(4-methylpiperazino)aniline (0.27 g, 1.41 mmol) were stirred at 150° C. for 4 h. The cooled reaction mixture was taken up in dichloromethane (50 mL) and washed with 10% NaOH (1×25 mL) then with water (1×20 mL). The organic layer was dried over sodium sulfate, filtered and evaporated. The residue was purified by column chromatography using chloroform:methanol (100:3) as eluent to give the title compound as a yellow solid (0.32 g, 0.58 mmol, 41%). ESMS m/z 553 (M+H)⁺; ¹H NMR (400 MHz, CDCl₃) δ ppm 8.53 (s, 1H), 7.65 (d, J=1.8 Hz, 1H), 7.52-7.59 (m, 3H), 7.45 (dd, J=8.2, 1.9 Hz, 1H), 7.28 (d, J=8.2 Hz, 1H), 6.97 (d, J=8.8 Hz, 2H), 4.48 (q, J=7.0 Hz, 2H), 3.17-3.26 (m, 4H), 2.55-2.70 (m, 4H), 2.37 (s, 3H), 1.37 (t, J=7.0 Hz, 3H).

Step 4: Synthesis of 6-[2-chloro-4-(thiophen-2-yl)phenyl]-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (13)

6-(4-Bromo-2-chlorophenyl)-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (12, 50 mg, 0.09 mmol), thiophene-2-boronic acid (35 mg, 0.27 mmol), K₃PO₄ (57 mg, 0.27 mmol) and PdCl₂(dppf) (7 mg, 0.01 mmol) were mixed as solids and placed under argon. Argon was bubbled through a mixture of dimethylformamide:water (20:1, 2.0 mL) for 20 min. The solvent was added to the solid and the suspension was heated under microwave irradiation at 140° C. for 30 min. The reaction mixture was evaporated and the crude product was purified by column chromatography, eluting with dichloromethane:methanol (100:3). The product was triturated with refluxing acetonitrile to yield the title compound as a yellow solid (48 mg, 0.09 mmol, 100%). ESMS m/z 557 (M+H)⁺; ¹H NMR (400 MHz, CDCl₃)₈ ppm 8.54 (s, 1H), 7.72 (s, 1H), 7.51-7.60 (m, 4H), 7.40 (d, J=8.0 Hz, 1H), 7.30-7.36 (m, 2H), 7.27 (s, 1H), 7.08-7.13 (m, 1H), 6.97 (d, J=8.8 Hz, 2H), 4.50 (q, J=7.0 Hz, 2H), 3.16-3.28 (m, 4H), 2.54-2.69 (m, 4H), 1.39 (t, J=7.0 Hz, 3H).

Step 4′: Synthesis of 6-[2-chloro-4-(thiophen-2-yl)phenyl]-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one hydrochloride (13)

6-(4-Bromo-2-chlorophenyl)-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (12, 666 mg, 1.2 mmol), thiophene-2-boronic acid (192 mg, 1.5 mmol), NaHCO₃ (504 mg, 6 mmol), Pd(PPh₃)₄ (50 mg), dioxane (30 mL) and water (6 mL) were placed in a microwave tube and flushed with argon. The reaction was heated by microwave at 140° C. for 1.5 h and was monitored by LC/MS. The reaction mixture was evaporated and the solid was extracted with chloroform (100 mL). The solids were removed and the filtrate was evaporated and purified by silica gel chromatography (CHCl₃+5% MeOH) to afford the title compound (82 mg, 5%) as a yellow solid. LCMS m/z 557 (M+H)⁺, Rt 1.84 min.

The hydrochloride salt was prepared by addition of an excess of hydrogen chloride in dioxane to a solution of free base in chloroform with quantitative yield. LCMS m/z 557 (M+H)⁺; Rt 1.84 min.

Examples 3-4b

The following compounds were made by the method of Example 2 using the appropriate arylacetic ester at Step 1 and aniline at Step 3. Examples containing secondary amines on the aniline were synthesized using the appropriate Boc protected aminoaniline and in the final step were treated with a solution of hydrogen chloride in an organic solvent to produce the example compound, usually isolated as the hydrochloride salt. In this manner, Example 3 was prepared using methyl 2-[5-methyl-2-(n-tert-butoxycarbonylpiperidine)-1,3-thiazol-4-yl]acetate and 4-(4-methylpiperazino)aniline. Example 4a and Example 4b were prepared from Example 3 by reductive methylation and treatment with acetic anyhydride respectively.

LCMS LCMS Ex. Structure MW Method Ion Rt 3

544.7 B 545 0.96 4a

558.8 B 560 0.98 4b

586.8 B 587 1.16

Examples 5-9 Preparation of Intermediate Compounds Synthesis of 2-(4-Amino-phenyl)-morpholine-4-carboxylic acid tert-butyl ester (25)

Step 1: Synthesis of 2-(4-nitro-phenyl)-oxirane (21)

To an ice-cold stirred suspension of 4-nitrophenacyl bromide 20 (80 g, 0.33 mol) in methanol (800 mL) was added sodium borohydride (13.64 g, 0.36 mol) in small portions. After stirring for 2 h at 0-5° C., potassium carbonate (45.20 g, 0.33 mol) was added in small portions at the same temperature. The suspension was stirred for 18 h at room temperature, diluted with brine (600 mL) and extracted with diethyl ether (600 mL, 500 mL), the combined organic layers were dried over sodium sulfate, filtered and evaporated. The title compound (54.87 g, 0.33 mmol, 100%) was obtained as a pale yellow solid.

Step 2: Synthesis of 2-(2-hydroxy-ethylamino)-1-(4-nitro-phenyl)-ethanol (22)

The mixture of 2-(4-nitro-phenyl)-oxirane 21 (24.1 g, 0.15 mol) in ethanolamine (500 mL) was stirred at 40° C. for 2 h, then room temperature for 18 h. The reaction was partitioned between ethyl acetate (200 mL) and water (200 mL) and the aqueous layer was extracted with ethyl acetate (4×100 mL). The combined organic layers were dried over sodium sulfate, filtered and evaporated. The residue was triturated with acetonitrile and collected to give the title compound 22 (19.80 g, 0.09 mmol, 60%) as a white solid. ESMS m/z 227 (M+H)⁺;

Step 3: Synthesis of (2-hydroxyethyl)-[2-hydroxy-2-(4-nitro-phenyl)-ethyl]-carbamic acid tert-butyl ester (23)

To 2-(2-hydroxy-ethylamino)-1-(4-nitro-phenyl)-ethanol (10.00 g, 44.2 mmol) in dichloromethane (80 mL) was added triethylamine (6.15 mL, 4.46 g, 44.2 mmol) followed by di-tert-butyl dicarbonate (9.65 g, 44.2 mmol) dissolved in dichloromethane (20 mL). The reaction mixture was stirred for 4 h at room temperature, washed with water (50 mL), and the aqueous layer was back extracted with ethyl acetate (2×50 mL). The combined organic layers were dried over sodium sulfate, filtered and evaporated. The residue was triturated with diisopropyl ether and collected. The title compound (12.04 g, 36.9 mmol) was obtained as a white solid. ESMS m/z 349 (M+Na)⁺;

Step 4: Synthesis of 2-(4-nitro-phenyl)-morpholine-4-carboxylic acid tert-butyl ester (24)

To an ice-cold stirred mixture of (2-hydroxyethyl)-[2-hydroxy-2-(4-nitro-phenyl)-ethyl]-carbamic acid tert-butyl ester 23 (5.50 g, 16.8 mmol) and triphenylphosphine (5.17 g, 19.7 mmol) in toluene (80 mL) was added triethylamine (6.15 mL, 4.46 g, 44.2 mmol) followed a solution of di-tert-butylazodicarboxylate (3.10 mL, 19.7 mmol) in toluene (30 mL) dropwise. The reaction mixture was stirred for 18 h at room temperature, washed with water (50 mL), and the aqueous layer was back extracted with ethyl acetate (2×50 mL). The combined organic layers were dried over sodium sulfate, filtered and evaporated. The residue was purified by column chromatography eluting with dichloromethane, The obtained product was triturated with diisopropyl ether and collected. The title compound (3.56 g, 11.5 mmol, 68%) was obtained as a white solid. ESMS m/z 253 (M+H−tBu)⁺; ¹H NMR (400 MHz, CDCl₃) δ ppm 8.23 (d, J=8.3 Hz, 2H) 7.57 (d, J=8.3 Hz, 2H) 4.53 (d, J=10.3 Hz, 1H) 4.20 (br. s., 1H) 4.06 (d, J=11.3 Hz, 1H) 3.94 (br. s., 1H) 3.66-3.75 (m, 1H) 3.06 (br. s., 1H) 2.76 (br. s., 1H) 1.50 (s, 9H).

Step 5: Synthesis of 2-(4-amino-phenyl)-morpholine-4-carboxylic acid tert-butyl ester (25)

A mixture of 2-(4-nitro-phenyl)-morpholine-4-carboxylic acid tert-butyl ester 24 (20 mg, 0.064 mmol) and Pd/C (5%, 2 mg) in methanol was stirred under hydrogen for 24 h. The catalyst was filtered off and washed with methanol. The filtrate was evaporated to give the title compound as an off-white solid (14 mg, 0.050 mmol, 78%). ESMS m/z 223 (M+H−tBu)⁺; ¹H NMR (400 MHz, DMSO-d₆) δ ppm 7.00 (d, J=8.3 Hz, 2H) 6.52 (d, J=8.3 Hz, 2H) 5.04 (s, 2H) 4.15 (dd, J=10.5, 2.5 Hz, 1H) 3.88 (dd, J=11.8, 2.5 Hz, 1H) 3.76 (d, J=12.8 Hz, 2H) 3.48 (td, J=11.7, 2.8 Hz, 1H) 2.92 (br. s., 1H) 2.77 (br. s., 1H) 1.41 (s, 9H).

Synthesis of 2-(4-aminophenyl)-thiomorpholine-S,S-dioxide-4-carboxylic acid tert-butyl ester (31)

Step 1: Synthesis of [2-nitro-1-(4-nitrophenyl)-ethylsulfanyl]-acetic acid methyl ester (27)

To an ice-cold stirred solution of 1-nitro-4-(2-nitro-vinyl)-benzene 26 (5.26 g, 27.09 mmol) and triethylamine (4.45 ml, 31 mmol) in tetrahydrofuran (80 mL) mercaptoacetic acid methyl ester (2.75 mL, 30.7 mmol) was added in one portion. The reaction mixture was stirred for 3 min, and c.HCl (3.55 mL) was added. The precipitated triethylamine HCl salt was removed by filtration through Perlite. The filtrate was evaporated and the residue was taken up in dichloromethane (100 mL), washed with 1M HCl (20 mL), water (2×20 mL), dried over sodium sulfate and evaporated. The title compound 27 was obtained as a pink low melting point crystalline material (8.11 g, 27 mmol, 99%). ESMS m/z 323 (M+Na)⁺.

Step 2: Synthesis of 6-(4-nitrophenyl)-thiomorpholin-3-one (28)

Acetic acid (240 mL) was heated to 72° C., then Zn (25.38 g, 388 mmol) and [2-nitro-1-(4-nitrophenyl)-ethylsulfanyl]-acetic acid methyl ester 27 (3.00 g, 10.00 mmol) were added in one portion. The well-stirred suspension was heated at reflux for 0.5 h, filtered through activated charcoal and evaporated. Dichloromethane (50 mL), water (30 mL) and 10% aqueous NaOH (50 mL) were added. The suspension was filtered through Perlite, the layers were separated and the aqueous layer was extracted with dichloromethane (2×20 mL). the combined organic layers were washed with water (10 mL), dried over sodium sulfate and evaporated. The residue was triturated with chloroform (10 mL) and the solid was collected and washed with chloroform (2 mL) to obtain the title product (0.225 g, 1.08 mmol, 11%). ESMS m/z 209 (M+H)⁺.

Step 3: Synthesis of 6-(4-nitrophenyl)-thiomorpholine (29)

To the solution of 6-(4-nitrophenyl)-thiomorpholin-3-one 28 (0.26 g, 1.25 mmol) in anhydrous tetrahydrofuran (6.7 mL) was added lithium aluminium hydride (0.16 g, 4.27 mmol) in several portions and the mixture was stirred at 60° C. for 2 h. Na₂SO₄×10H₂O (2.00 g) was added in small portions until the complex was decomposed. The suspension was filtered, the solids were washed with tetrahydrofuran (2×3 mL) and the combined filtrates were evaporated. The title compound was obtained as a viscous oil (0.25 g, 1.26 mmol, 100%). ESMS m/z 195 (M+H)⁺.

Step 4: Synthesis of 2-(4-aminophenyl)-thiomorpholine-4-carboxylic acid tert-butyl ester (30)

To 6-(4-nitrophenyl)-thiomorpholine 29 (0.25 g, 1.26 mmol) in anhydrous tetrahydrofuran (2.5 mL) was added di-tert-butyl dicarbonate (0.25 g, 1.14 mmol). The solution was stirred at room temperature for 1 h. After evaporation, the residue was purified by column chromatography using hexane:ethyl acetate (4:1→3:1→2:1). The title compound 30 was obtained as a white crystalline solid (0.13 g, 0.42 mmol, 33%). ESMS m/z 317 (M+Na)⁺; ¹H NMR (400 MHz, DMSO-d₆) δ ppm 6.99 (d, J=8.3 Hz, 2H) 6.51 (d, J=8.3 Hz, 2H) 5.07 (s, 2H) 4.23 (d, J=13.6 Hz, 1H) 4.12 (br. s., 1H) 3.69 (dd, J=10.8, 2.8 Hz, 1H) 3.13 (br. s., 1H) 2.98 (br. s., 1H) 2.73 (td, J=12.7, 3.0 Hz, 1H) 2.56 (d, J=13.6 Hz, 1H) 1.40 (s, 9H).

Step 5: Synthesis of 2-(4-aminophenyl)-thiomorpholine-S,S-dioxide-4-carboxylic acid tert-butyl ester (31)

To a solution of 2-(4-amino-phenyl)-thiomorpholine-4-carboxylic acid tert-butyl ester 30 (150 mg, 0.51 mmol) in dichloromethane (10 mL) was added a solution of 3-chloroperbenzoic acid (77%, 258 g, 1.15 mmol) in dichloromethane (11 mL) at 0-5° C. and the mixture was stirred at 0-5° C. for 1.5 h. The reaction mixture was washed with 10% aqueous sodium bicarbonate (20 mL), the organic layer was dried over sodium sulfate, filtered and evaporated. The residue was purified by column chromatography using dichloromethane:methanol (100:2). The title compound 31 was obtained as a pale yellow solid (120 mg, 0.37 mmol, 72%). ESMS m/z 271 (M+H−tBu)⁺.

Examples 5-9

The following compounds were made by the method of Example 2 using the appropriate arylacetic ester at Step 1 and aniline at Step 3. Examples containing secondary amines on the aniline were synthesized using the appropriate Boc protected aminoaniline and in the final step a small portion was treated with a solution of hydrogen chloride in an organic solvent to produce the example compound, usually isolated as the hydrochloride salt.

Ex. Structure MW LCMS Method LCMS Ion Rt 5

519.5 B 519 1.39 6

540.9 B 540 1.38 7

556.9 B 556 1.49 8

570.9 B 572 1.51 9

588.9 B 588 1.68

Example 10 Synthesis of 6-(4-bromo-2-chlorophenyl)-8-ethyl-2-[4-(4-methyl-thiomorpholin-2yl)-phenylamino]-8H-pyrido[2,3-d]pyrimidin-7-one hydrochloride (32)

To a suspension of 6-(4-bromo-2-chlorophenyl)-8-ethyl-2-(4-thiomorpholin-2-yl-phenylamino)-8H-pyrido[2,3-d]pyrimidin-7-one dihydrochloride (40 mg, 0.06 mmol) in dichloromethane (1 mL) was added triethylamine (18 μL, 12.9 mg, 0.13 mmol), benzotriazole (8 mg, 0.07 mmol) and formaldehyde (37%, 5.7 μL, 0.08 mmol) and the reaction was stirred at room temperature for 2 h. Sodium borohydride (4.5 mg, 0.12 mmol) was added and the reaction mixture was stirred for 18 h, diluted with dichloromethane (10 mL) and washed with sodium bicarbonate solution (10%, 10 mL). The aqueous layer was back extracted with dichloromethane (5×5 mL), and the combined organic layers were washed with water (10 mL), dried over sodium sulfate, filtered and evaporated in vacuo. The crude product was purified by column chromatography eluting with dichloromethane:2-propanol (100:5) to give a white solid (6.8 mg, 0.01 mmol, 17%). The free base was dissolved in dichloromethane (3 mL), treated with HCl/diethyl ether (0.445 M, 27 μl, 0.01 mmol), stirred at room temperature for 18 h and evaporated to give the title compound 32 as a solid (7.7 mg, 0.01 mmol, 100%). ESMS m/z 570/572 (M+H)⁺; ¹H NMR (400 MHz, DMSO-d₆) δ ppm 10.00 (s, 1H) 8.82 (s, 1H) 7.82-7.89 (m, 3H) 7.78 (s, 1H) 7.61 (dd, J=8.4, 1.6 Hz, 1H) 7.33-7.41 (m, 3H) 4.30-4.48 (m, 3H) 3.60 (br. s., 2H) 3.24 (br. s., 2H) 2.93 (br. s., 2H) 2.77 (br. s., 3H) 1.32 (t, J=6.9 Hz, 3H).

Example 11 6-(4-bromo-2-chlorophenyl)-8-ethyl-2-(4-(4-methylthiomorpholin-2-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one

The following compound was made by the method of Example 10 starting with the compound from Example 6.

LCMS Ex Structure MW Method LCMS Ion Rt 11

554.9 B 556 1.42

Examples 12-39

The following compounds were made by the method of Example 2 using the appropriate arylacetic ester at Step 1, aniline at Step 3 and boronic acid or ester at Step 4. Examples containing secondary amines on the aniline were synthesized using the appropriate Boc protected aminoaniline and in the final step were treated with a solution of hydrogen chloride in an organic solvent to produce the example compound, usually isolated as the hydrochloride salt. Reaction of 6-(4-bromo-2-chlorophenyl)-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (12) with cyclopropylboronic acid generated a mixture of products which were separated to provide examples 13 and 14.

Ex. Structure MW LCMS Method LCMS Ion Rt 12

480.6 B 481 1.41 13

515.1 B 515 1.45 14

520.7 B 521 1.49 15

557.1 B 557 1.47 16

522.7 B 523 1.47 17

571.1 B 571 1.60 18

541.1 B 541 1.45 19

541.1 B 541 1.44 20

552.1 B 552 1.06 21

552.1 B 552 1.16 22

553.1 B 553 1.21 23

540.0 B 540 1.20 24

554.1 B 554 1.24 25

506.6 C 507 1.38 26

506.6 C 507 1.39 27

555.1 C 555 1.58 28

520.6 C 521 1.58 29

590.1 C 590 1.84 30

602.1 C 602 1.54 31

520.6 C 521 1.52 32

522.7 C 523 1.85 33

570.1 C 570 1.71 34

506.6 C 507 1.76 35

547.7 C 548 1.65 36

609.1 C 609 1.47 37

518.6 C 519 1.48 38

567.7 C 568 1.53 39

582.1 C 582 1.69

Example 40 Synthesis of 6-(2-chloro-4-(1H-pyrazol-4-yl)phenyl)-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (33) Preparation of Intermediate Compounds

Step 1: Synthesis of 6-(4-bromo-2-chlorophenyl)-8-ethyl-2-(methylthio)pyrido[2,3-d]pyrimidin-7(8H)-one (10)

Compound 9 (115.1 g, 583.3 mmol) and methyl 2-(4-bromo-2-chlorophenyl)acetate 19 (160.1 g, 641.7 mmol, 1.1 eq) were added to suspension of K₂CO₃ (241.5 g, 1.75 mol, 3 eq.) in 1000 mL of dry DMF at r.t. The reaction mixture was stirred at 70° C. for 12 h under argon. Then the reaction mixture was cooled to r.t. and poured into 2 L of water. The product was precipitated at standing over 2 h at r.t. The solid was filtered and washed with hot EtOH. Product 10 was obtained as brown solid after recrystallization from CHCl₃-EtOH (419 g, 93%).

¹H NMR (400 MHz, DMSO-d₆) ppm: 1.30 (t, 3H, J=6.86 Hz), 2.62 (s, 3H), 4.42 (q, 2H, J=6.86 Hz), 7.34 (d, 1H, J=8.18 Hz), 7.59 (d, 1H, J=6.41 Hz), 7.73 (d, 1H, J=1.55 Hz), 7.95 (s, 1H), 8.90 (s, 1H).

Step 2: Synthesis of 6-(4-bromo-2-chlorophenyl)-8-ethyl-2-(methylsulfinyl)pyrido[2,3-d]pyrimidin-7(8H)-one (11)

To a solution of 10 (110.0 g, 267.8 mmol) in dichloromethane (600 mL) was added dropwise a solution of 3-chloroperbenzoic acid (70%, 66.04 g, 267.8 mmol) in dichloromethane (400 mL) at 0-5° C. and the mixture was stirred for 1 h. at r.t. The reaction mixture was washed with saturated sodium bicarbonate solution (2×200 mL) and water (200 mL), and the organic layer was dried over sodium sulfate, filtered and evaporated. Compound 11 was obtained as brown solid and used without purification (108.6 g, 95%).

¹H NMR (400 MHz, DMSO-d₆) ppm: 1.32 (t, 3H, J=6.86 Hz), 2.95 (s, 3H), 4.48 (q, 2H, J=6.86 Hz), 7.37 (d, 1H, J=8.18 Hz), 7.62 (d, 1H, J=7.96 Hz), 7.76 (s, 1H), 8.15 (s, 1H), 9.26 (s, 1H).

Step 3: General Procedure for Amination

Corresponding anilines 34a-p (1.2 mmol) and sulfoxide 11 (1.0 mmol) were dissolved in chloroform (10 mL). The solvent was then evaporated under reduced pressure. The resulting homogenized reaction mixture was heated in an oil bath at 120° C. for 3 h. The cooled residue was suspended in MeOH/Et₂O, solid was filtered and purified by silica gel chromatography to afford the target compounds 35a-p.

Step 4: General Procedure for Suzuki Coupling

To a solution of bromide 35a-p (1.0 mmol) and appropriate aryl-boronic acid (1.2 mmol) in dioxane (50 mL) under argon, a solution of Na₂CO₃ (5.0 mmol) in water (25 mL) and tetrakis(triphenylphosphine)palladium (5% mol.) were added successively under vigorous stirring. The reaction mixture was heated to reflux for 18 h. The resulting mixture was evaporated to dryness and the solid was extracted with chloroform (3×50 ml), combined organic layers dried by Na₂SO₄, concentrated in vacuo and chromatographed on silica gel. Recrystallization from i-PrOH/CHCl₃ afforded the desired compounds as free bases. 6N HCl (10 mL) was added and stirred for 20 min. Solution was filtered through celite, evaporated under reduced pressure and dried in vacuum oven at 60° C. to afford final compounds 36a-p.

General Procedure Aniline Intermediates 34a-p 4-(piperazin-1-yl)anilines 34a-h

Step 1: 4-(4-nitrophenyl)piperazines

The mixture of corresponding 4-nitro-chlorobenzene 37 and appropriate piperazines (2 eq) was heated at 140° C. overnight. The solution was poured into a saturated solution of K₂CO₃ in water. The precipitate was filtered, washed with water and dried.

Step 2: 4-(piperazin-1-yl)anilines

To a mixture of amine 38 and N₂H₄—H₂O (5 eq) in EtOH was added Ni/Ra (0.07 eq). The suspension was heated at 50° C. overnight, filtered via celite, the celite was additionally washed with EtOH. The filtrate was evaporated and dried under vacuum to provide piperazines 34a-h.

3-(4-Aminophenyl)pyrrolidines 34i-j

Steps 1-2: 3-(4-(dibenzylamino)phenyl)-2,5-dihydro-1H-pyrroles (41)

To a solution of N,N-dibenzyl-4-bromoaniline 39 (49 g, 0.14 mol) in 1000 mL of anhydrous THF, cooled to −85° C., 70 ml of n-BuLi hexane solution (2.5 M, 0.17 mol, 1.2 eq.) was added dropwise with stirring under argon atmosphere over a period of 20 minutes. Stirring was continued for 30 minutes at −85° C. A solution of t-butyl 3-oxopyrrolidine-1-carboxylate was then added dropwise over a period of 1 hour. The reaction mixture was allowed to warm up overnight with stirring. The resulting yellow solution was diluted with 500 mL of water and neutralized with 150 mL of 1 M HCl to pH 6-7. The organic layer was separated and aqueous phase was extracted with 500 ml of EtOAc. Combined organic phases were washed with brine and evaporated. The resulting brownish slurry was chromatographed on silica gel to provide 41 (13 g, 20% yield). ¹H NMR (400 MHz, DMSO-d6) δ ppm: 1.44 (s, 9H), 4.13 (m, 2H), 4.27 (m, 2H), 4.69 (s, 4H), 5.93-5.96 (m, 1H), 6.64 (d, J=7.0 Hz, 2H), 7.15 (t, J=8.2 Hz, 2H), 7.20-7.24 (m, 6H), 7.29-7.33 (m, 2H).

Step 3: 3-(4-Aminophenyl)pyrrolidine 34i

Amine 41 (13 g, 0.03 mol), was dissolved in ethanol (100 mL) and water (20 mL). 10% Pd/Carbon (2.0 g) was added and the reaction mixture was stirred overnight under hydrogen gas (30 atm). The crude product obtained after filtration and evaporation was purified on silica gel to afford 34i (2.9 g, 37% yield).

¹H NMR (400 MHz, DMSO-d6) δ ppm: 1.42 (s, 9H), 1.80-1.93 (m, 1H), 2.10-2.16 (m, 1H), 3.05-3.10 (m, 1H), 3.13-3.19 (m, 1H), 3.22-3.32 (m, 1H), 3.45-3.49 (m, 1H), 3.60-3.65 (m, 1H), 4.62 (br. s, 2H), 6.50 (d, J=8.3 Hz, 2H), 6.88 (d, J=8.1 Hz, 2H).

34j was synthesized using the method outlined in the synthesis of 34i using N,N-dibenzyl-4-bromo-3-fluoroaniline in step 1.

3-(4-Aminophenyl)piperidines 34 k-p

3-(4-Aminophenyl)piperidines 34k-p were prepared in two step procedure as described for the 3-(4-Aminophenyl)pyrrolidines 34i-j to afford the piperidines 34k-p.

Entry Compound 34a

34b

34c

34d

34e

34f

34g

34h

Entry Compound 34i

34j

34k

34l

34m

34n

34o

34p

Examples 40-82

The following compounds were made by the method of Example 40 using the appropriate arylacetic ester at Step 1, appropriate aniline at Step 3 and appropriate boronic acid in step 4. Examples containing secondary amines on the aniline were synthesized using the appropriate Boc protected aminoaniline in step 3 and in the final step were treated with a solution of trifluoroacetic acid in an organic solvent or 6N hydrochloric acid to remove the Boc protecting group, followed by isolating as free base by washing with aqueous potassium carbonate solution to produce the example compounds 40-82, usually isolated as free base or further converted to hydrochloride salts.

LCMS LCMS Ex. Structure MW Method ION Rt 40

541.1 C 541 1.48 41

556.0 C 556 1.34 42

557.0 C 558 1.60 43

539.0 C 540 1.49 44

585.1 C 585 1.62 45

538.1 C 539 1.32 46

570.1 C 571 1.40 47

571.1 C 571 1.52 48

599.1 C 600 1.56 49

581.1 C 582 1.52 50

566.1 C 567 1.37 51

580.1 C 580 1.34 52

598.1 C 598 2.03 53

567.1 C 568 1.59 54

580.1 C 580 1.36 55

584.1 C 584 1.49 56

537.1 C 538 1.28 57

555.1 C 555 1.15 58

566.1 C 566 1.30 59

566.1 C 567 0.99 60

609.1 C 609 1.30 61

566.1 C 567 1.04 62

523.0 C 524 1.07 63

609.1 C 609 1.33 64

608.2 C 608 1.62 65

551.1 C 551 1.10 66

565.1 C 566 1.11 67

608.2 C 609 1.33 68

565.1 C 566 1.08 69

565.1 C 566 1.06 70

565.1 C 565 1.04 71

579.2 579 1.09 72

579.2 C 579 1.11 73

525.1 C 526 1.34 74

594.1 C 595 1.37 75

579.2 C 579 1.06 76

511.0 C 511 1.04 77

551.1 C 551 1.27 78

551.1 C 551 1.30 79

551.1 C 551 1.27 80

583.1 C 583 1.19 81

569.1 C 569 1.25 82

569.1 C 569 1.32

Example 83 Synthesis of 6-[2-chloro-4-(5-methylthiazol-2-yl)phenyl]-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (46)

6-(4-Bromo-2-chlorophenyl)-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (12, 194 mg, 0.35 mmol), 5-methyl-2-(tributylstannyl)-thiazole (171 mg, 0.44 mmol), Pd(PPh₃)₄ (50 mg) and toluene (15 mL) were placed in a microwave tube and flushed with argon. The reaction was heated by microwave at 140° C. for 1.5 h. The reaction mixture was evaporated and the solid was extracted with chloroform (100 mL). The solids were removed and the filtrate was evaporated and purified by silica gel chromatography (CHCl₃/5% MeOH) to afford the compound 46 (17 mg, 8%) as yellow solid. LCMS m/z 572 (M+H)⁺; Rt 1.74 min; ¹H NMR (400 MHz, CDCl₃) δ ppm 8.56 (s, 1H), 8.04 (s, 1H), 7.81-7.83 (d, 1H), 7.54-7.61 (m, 4H), 7.46-7.48 (d, 1H), 7.32-7.37 (s, 1H), 6.97-7.00 (d, 2H), 4.48-4.54 (q, 2H), 3.24-3.26 (m, 4H), 2.63-2.65 (m, 4H), 2.54 (s, 3H), 2.40 (s, 3H), 1.38-1.42 (t, 3H).

Examples 84-85

The following compounds were made by the method of Example 83 using the appropriate heteroarylstannane. Final compounds were treated with a solution of hydrogen chloride to produce the example compounds as hydrochloride salt.

LCMS LCMS Ex. Structure MW Method Ion Rt 84

558.1 C 558 1.65 85

558.1 C 558 1.60

Examples 86-95

The compounds in examples 86-95 were made using the method described in example 40 in steps 1-3 and the final step described below.

To a solution of appropriate aryl bromide (1.0 mmol) in toluene (50 ml) under argon atmosphere respective tributyltin-aryl (1.1 mmol) and tetrakis(triphenylphosphine)palladium (5% mol.) were added successively. The reaction mixture was heated to reflux for 12-24 h, resulting mixture was evaporated to dryness, dissolved in chloroform (50 ml), filtered through celite, concentrated in vacuo and chromatographed on silica gel. Recrystallization from i-PrOH/CHCl₃ afforded compounds as free bases. 6N HCl (10 ml) was added and stirred for 20 min. The solution was filtered through celite, evaporated under reduced pressure and dried in vacuum oven at 60° C. to afford the products from examples 86-95 as hydrochloride salts.

Examples 86-95

Examples 86-95 were made by the method of example 40 using the appropriate arylacetic ester in step 1, the appropriate aniline in step 3, and the appropriate aryl stannane in the step shown in the example above. Examples containing secondary amines on the aniline were synthesized using the appropriate Boc protected aminoaniline in step 3 and in the final step were treated with a solution of trifluoroacetic acid in an organic solvent or 6N hydrochloric acid to remove the Boc protecting group, followed by isolating as free base by washing with aqueous potassium carbonate solution to produce the example compounds isolated as free base or further converted to hydrochloride salts.

LCMS Ex. Structure Method MW LCMS Ion Rt 86

C 523.7 524 1.57 87

C 558.1 558 1.64 88

C 572.1 572 1.74 89

C 553.1 553 1.70 90

C 562.1 563 1.69 91

C 590.1 591 1.65 92

C 586.2 587 1.67 93

C 544.1 545 1.59 94

C 572.1 573 1.62 95

C 572.1 573 1.66

Examples 96-101

The compounds in examples 96-101 were made using the method described in example 40 in steps 1-3 and the final step described below.

To a suspension of bromide (0.98 mmol) in DMA (20 ml) under argon, thiazole (1.47 mmol), potassium acetate (1.47 mmol) and tetrakis(triphenylphosphine)palladium (5% mol.) were added successively. The reaction was carried out in pressure vessel at 120° C. for 12 hours. The solvent was evaporated under reduced pressure, water (50 ml) was added to the residue and stirred for 1 h. Solid was filtered off and purified by flash chromatography on silica gel. Recrystallization from i-PrOH/CHCl₃ afforded the target compounds as free bases. 6N HCl (10 ml) was added and stirred for 20 min. Solution was filtered through celite, evaporated under reduced pressure and dried in vacuum oven at 60° C. to afford compounds shown in examples 96-101 as HCl salts.

LCMS LCMS Ex. Structure Method MW Ion Rt  96

C 558.1 558 1.63  97

C 586.2 587 1.65  98

C 600.2 601 1.76  99

C 572.1 573 1.68 100

C 576.1 576 1.68 101

C 571.2 572 1.43

Example 102 Synthesis of 6-[2,2′]bithiophenyl-5-yl-8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-8H-pyrido[2,3-d]pyrimidin-7-one (49)

Step 1: Synthesis of 8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-7-oxo-7,8-dihydro-pyrido[2,3-d]pyrimidine-6-boronic acid (48)

6-Bromo-8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-8H-pyrido[2,3-d]pyrimidin-7-one (47, 100 mg, 0.22 mmol), bis(pinacolato)diboron (63 mg, 0.25 mmol), potassium acetate (66 mg, 0.68 mmol) and PdCl₂(PPh₃)₂ (16 mg, 0.02 mmol) were mixed under argon in degassed toluene (3 mL). The resulting suspension was irradiated for 30 min at 120° C. in a microwave reactor. After completion, the reaction mixture was evaporated and the residue was purified by column chromatography using dichloromethane:methanol:triethylamine (4:1:0→1:1:0→0:95:5) as eluent. The crude product was dissolved in dichloromethane (10 mL), washed with water (5 mL), and the organic layer was dried over sodium sulfate, filtered and evaporated. The title compound 48 (15 mg, 0.04 mmol, 18%) was obtained as a yellow solid. ESMS m/z 409 (M+H)⁺; ¹H NMR (400 MHz, DMSO-d₆) δ ppm 10.07 (br. s., 8.85 (s, 1H) 8.52 (s, 2H) 8.28 (s, 1H) 7.64 (br. s., 2H) 6.94 (d, J=9.0 Hz, 2H) 4.33 (q, J=6.9 Hz, 2H) 3.07-3.14 (m, 4H) 2.43-2.47 (m, 4H) 2.22 (s, 3H) 1.26 (t, J=6.9 Hz, 3H).

Step 2: Synthesis of 6-[2,2′]bithiophenyl-5-yl-8-ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-8H-pyrido[2,3-d]pyrimidin-7-one (49)

8-Ethyl-2-[4-(4-methyl-piperazin-1-yl)-phenylamino]-7-oxo-7,8-dihydro-pyrido[2,3-d]-pyrimidine-6-boronic acid (48, 71 mg, 0.17 mmol), 5-bromo-2,2′-bithiophene (47 mg, 0.19 mmol), sodium carbonate (55 mg, 0.52 mmol) and Pd(PPh₃)₄ (20 mg, 0.02 mmol) were mixed under argon in a degassed mixture of dimethoxyethane:water (10:1, 3 mL). The resulting suspension was irradiated for 60 min at 120° C. in a microwave reactor. After completion, the reaction mixture was evaporated and the residue was purified by column chromatography using dichloromethane:methanol (100:5) as eluent. The collected product was triturated with ethyl acetate (10 mL) and collected. The title compound 49 (30 mg, 0.057 mmol, 33%) was obtained as a yellow solid. ESMS m/z 529 (M+H)⁺; ¹H NMR (400 MHz, DMSO-d₆) δ ppm 9.99 (br. s., 1H) 8.81 (s, 1H) 8.45 (s, 1H) 7.72 (d, J=3.8 Hz, 1H) 7.66 (br. s., 2H) 7.51 (dd, J=5.1, 0.9 Hz, 1H) 7.31-7.37 (m, 2H) 7.11 (dd, J=5.0, 3.8 Hz, 1H) 6.95 (d, J=9.0 Hz, 2H) 4.42 (q, J=7.0 Hz, 2H) 3.04-3.17 (m, 4H) 2.42-2.48 (m, 4H) 2.23 (s, 3H) 1.31 (t, J=7.0 Hz, 3H).

Examples 103-110

The following compounds were made by the method of Example 102 using the appropriate heteroaryl bromide at Step 2. Examples containing secondary amines on the aniline were synthesized using the appropriate Boc protected aminoaniline and in the final step were treated with a solution of hydrogen chloride in an organic solvent to produce the example compound, usually isolated as the hydrochloride salt.

LCMS LCMS Ex. Structure MW Method Ion Rt 103

486.6 B 487 1.46 104

543.7 B 544 1.39 105

528.6 B 529 1.34 106

523.7 B 524 1.29 107

523.7 B 524 1.21 108

514.6 B 515 1.13 109

523.7 B 524 1.05 110

513.6 B 514 1.33

Example 111 Synthesis of 6-(2-chloro-4-(1H-tetrazol-5-yl)phenyl)-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (51)

Step 1: Synthesis of 3-chloro-4-(8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl)benzonitrile (50)

Compound 12 (6 g, 10.83 mmol) was dissolved in 50 mL of dimethylformamide under argon and with stirring. 636 mg (5.42 mmol) of zinc cyanide, 1.21 g (2.17 mmol) of 1,1′-bis(diphenylphosphino)ferrocene (dppf) and 1.12 g (1.08 mmol) of tris(dibenzylideneacetone)dipalladium(0) (Pd₂(dba)₃) were then added. The mixture was heated at 70° C. under argon for 15 hours. The reaction medium was poured into 200 mL of water and the solid precipitate was filtered and dried. The solid was dispersed in a mixture of 50 mL of chloroform and 50 mL of MeOH and filtered through Celite.(R). The organic solution was then concentrated to dryness under reduced pressure. The residue was crystallized from EtOH to afford 50 (4.52 g, 83%) as a brown solid.

Step 2: 6-(2-chloro-4-(1H-tetrazol-5-yl)phenyl)-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (51)

A mixture of compound 50 (100 mg, 0.2 mmol) and sodium azide (90 mg, 1.4 mmol) in 1 mL of DMF was stirred at 100° C. for 15 h. The mixture was cooled down to r.t. and diluted with 5 mL of water. The solid precipitate was filtered and dried. The compound was purified by prep. HPLC to give the title compound 51 (41 mg, 75%). LCMS m/z 544 (M+H)⁺ Rt 1.46 min. ¹H NMR (400 MHz, DMSO-d₆): δ 10.08 (bs, 1H), 9.78 (bs, 1H), 8.81 (s, 1H), 8.19 (s, 1H), 8.07 (d, J=8.0 Hz, 1H), 7.94 (s, 1H), 7.73 (d, J=8.0 Hz, 2H), 7.67 (d, J=8.2 Hz, 1H), 7.03 (d, J=8.8 Hz, 2H), 4.38 (q, J=7.1 Hz, 2H), 3.86 (bm, 2H), 3.52 (bm, 2H), 3.18 (bm, 2H), 2.95 (bm, 2H), 2.87 (s, 3H). 1.30 (t, J=7.1 Hz, 3H).

Example 112 3-chloro-4-8-ethyl-2-[4-(4-piperazino)anilino]-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl-N¹-hydroxy-1-benzenecarboximidamide (53)

Step 1: 3-chloro-4-(8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl)-N-hydroxybenzimidamide (52)

A mixture of compound 50 (9.12 mmol), NH₂OH*HCl (23 mmol) and Na₂CO₃ (2.4 g, 23 mmol) in 200 mL of EtOH was stirred at 50° C. for 3 h. The mixture was cooled to r.t. and the solid precipitate was filtered, washed with EtOH, and H₂O. The solid was dried to give 52 which was used immediately without further purification.

Step 2: Ethyl 3-(3-chloro-4-(8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl)phenyl)-1,2,4-oxadiazole-5-carboxylate (53)

A mixture of compound 52 (150 mg, 0.28 mmol) and 54 (76 mg, 0.56 mmol) in 2 mL of pyridine was stirred at 90° C. for 2 h. The mixture was cooled to r.t. and diluted with water. The solid precipitate was filtered, washed with EtOH, H₂O and dried. The compound was purified by prep. HPLC purification to afford 53 as a TFA salt (15 mg, 8%). LCMS m/z 616 (M+H)⁺ Rt 1.69 min. ¹H NMR (400 MHz, DMSO-d₆) δ 8.53 (s, 1H), 8.32 (s, 1H), 8.13 (d, J=7.5 Hz, 1H), 7.71-7.61 (m, 3H), 7.55 (d, J=8.0 Hz, 1H), 7.27 (s, 1H), 6.99 (d, J=8.8 Hz, 2H), 4.60 (q, J=7.3 Hz, 2H), 4.49 (q, J=6.9 Hz, 2H), 3.81-3.57 (m, 5H), 3.39 (bm, 2H), 3.06 (m, 2H), 2.91 (s, 3H), 1.52 (t, J=7.3 Hz, 2H), 1.40 (t, J=6.9 Hz, 3H).

Example 113 6-(2-chloro-4-(5-(trifluoromethyl)-1,2,4-oxadiazol-3-yl)phenyl)-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (56)

A mixture of compound 52 (170 mg, 0.32 mmol), and 55 (334 mg, 1.59 mmol) in 2 mL of pyridine was stirred at 90° C. for 15 h. The mixture was cooled down to r.t. and diluted with water. The solid precipitate was filtered, washed with EtOH, H₂O and dried. The compound was purified by prep. HPLC. The compound was converted to its HCl using hydrochloric acid to afford the product 56 (40 mg, 20% yield). LCMS m/z 612 (M+H)+ Rt 1.86 min. ¹H NMR (400 MHz, DMSO-d6) δ ppm 10.09 (bs, 1H), 9.91 (bs, 1H), 8.81 (s, 1H), 8.15 (s, 1H), 8.09 (d, J=7.5 Hz, 1H), 7.96 (s, 1H), 7.79-7.67 (m, 3H), 7.03 (d, J=8.8 Hz, 2H), 4.38 (q, J=6.9 Hz, 2H), 3.80 (bm, 2H), 3.51 (bm, 2H), 3.18 (bm, 2H), 2.96 (bm, 2H), 2.87 (s, 3H), 1.30 (t, J=6.9 Hz, 3H).

Examples 114-117

The following compounds were made by the method of Example 113 using the appropriate acid anhydride. Examples containing secondary amines on the aniline were synthesized using the appropriate Boc protected aminoaniline and in the final step were treated with a solution of hydrogen chloride in an organic solvent to produce the example compound, usually isolated as the hydrochloride salt.

LCMS LCMS Ex. Structure MW Method Ion Rt 114

543.0 C 544 1.62 115

597.0 C 598 1.85 116

639.1 C 639 2.05 117

585.1 C 585 1.71

Example 118 6-(2-chloro-4-(5-methyl-1,2,4-oxadiazol-3-yl)phenyl)-8-ethyl-2-(4-(4-methylpiperazin-1-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (61)

Step 1: (3-chloro-4-[8-ethyl-2-(methylsulfanyl)-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl]benzonitrile) (57)

The mixture of compound 10 (20 g, 48.8 mmol), zinc cyanide (3.4 g, 28.9 mmol), Pd₂dba₃ (5 g, 4.8 mmol) and dppf (5.4 g, 9.7 mmol) in 300 mL anhydrous DMF was heated at 70° C. for 12 h in inert atmosphere. The solvent was concentrated under reduced pressure and the mixture was diluted with 1000 mL of water. The solid was separated by filtration, dried, suspended in 50 mL CH₂Cl₂ and stirred for 30 min. After filtration, the solid was washed with Et₂O and dried to afford the title compound 57 (11 g, 64%) as a white solid. 1H NMR (400 MHz, DMSO-d₆) δ ppm 8.94 (1H, s) 8.18 (1H, s), 8.08 (1H, s), 7.93 (1H, dd), 7.66 (1H, d, J=7.8 Hz), 4.40 (2H, q, J=7.3 Hz), 2.63 (3H, s), 1.25 (3H, t, J=7.0 Hz).

Step 2: (3-chloro-4-[8-ethyl-2-(methylsulfanyl)-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl]-N¹-hydroxy-1-benzenecarboximidamide) (58)

t-BuOK (17.3 g, 154 mmol) was added to a solution of NH₂OH HCl (16.3 g, 154 mmol) in 150 mL of DMSO at 5° C. and was stirred for 30 min. Compound 57 (11 g, 30.8 mmol) was added at room temperature and the reaction mixture was stirred 3-5 h. After completion of the reaction, the solution was added to 800 mL of water. A white solid was precipitate was collected by filtration, washed with water and dried to afford the title compound 58 (11.3 g, 94%). ¹H NMR (400 MHz, DMSO-d₆) δ ppm 9.84 (1H, s) 8.93 (1H, s), 8.02 (1H, s), 7.82 (1H, s), 7.72 (1H, dd), 7.43 (1H, d, J=8.1 Hz), 5.97 (2H, bs), 4.40 (2H, q, J=7 Hz), 2.63 (3H, s), 1.25 (3H, t, J=6.8 Hz).

Step 3: (6-[2-chloro-4-(5-methyl-1,2,4-oxadiazol-3-yl)phenyl]-8-ethyl-2-(methylsulfanyl)pyrido[2,3-d]pyrimidin-7(8H)-one) (59)

Compound 58 (5 g, 12.8 mmol) was dissolved in 50 mL of pyridine and cooled to 5-10° C. Acetyl chloride (1.2 g, 15.3 mmol) was added drop wise and the reaction mixture was heated at 90° C. overnight. The solvent was concentrated under reduced pressure and water and EtOAc were added to the mixture. The organic layer was separated, dried over sodium sulfate and then evaporated in vacuo. The title compound 59 was purified by column chromatography (CHCl₃) (3.9 g, 74%). ¹H NMR (400 MHz, DMSO-d₆) δ ppm 8.95 (1H, s) 8.11-8.07 (2H, m), 8.03 (1H, dd), 7.64 (1H, d, J=7.5 Hz), 4.40 (2H, q, J=7.0 Hz), 2.70 (3H, s), 2.63 (3H, s) 1.27 (3H, t, J=6.8 Hz).

Step 4: (6-[2-chloro-4-(5-methyl-1,2,4-oxadiazol-3-yl)phenyl]-8-ethyl-2-(methylsulfinyl)pyrido[2,3-d]pyrimidin-7(8H)-one) (60)

m-Chloroperbenzoic acid (2.68 g, 13.5 mmol, 87%) was carefully added at 5° C. to a solution of compound 59 (5.4 g, 13 mmol) in 100 mL CH₂Cl₂. The reaction mixture was stirred at room temperature 1 h. 100 mL saturated solution of K₂CO₃ was added and stirred for 10 min. The organic layer was separated, washed with water and dried over sodium sulfate and then evaporated in vacuo to afford the title compound 60 (5.5 g, 98%) as white solid. ¹H NMR (400 MHz, DMSO-d₆) δ ppm 9.28 (1H, s) 8.26 (2H, m), 8.11 (1H, d), 8.07 (1H, dd), 7.68 (1H, d, J=7.8 Hz), 4.45 (2H, q, J=7.0 Hz), 2.96 (3H, s), 2.71 (3H, s) 1.29 (3H, t, J=6.9 Hz).

Step 5: (6-[2-chloro-4-(5-methyl-1,2,4-oxadiazol-3-yl)phenyl]-8-ethyl-2-[4-(4-methylpiperazino)anilino]pyrido[2,3-d]pyrimidin-7(8H)-one) (61)

A mixture of compound 59 (9.2 g, 21.4 mmol) and 4-(4-methylpiperazino)aniline (6.1, 32.1 mmol) was heated at 140° C. for 10-12 h. After cooling, the reaction mixture was washed with EtOH and Et₂O and the solid was collected by filtration. Recrystallization was achieved from EtOH/CHCl₃. The free base was then dissolved in 20% HCl(aq) and evaporated to dryness, to afford the title compound 61 (11.2 g, 74% yield). LCMS m/z 557 (M+H)+ Rt 1.47 min. ¹H NMR (400 MHz, DMSO-d₆) δ ppm 11.41 (1H, bs), 10.18 (1H, bs), 8.82 (1H, s), 8.05 (1H, d), 7.99 (1H, dd), 7.93 (1H, s), 7.73 (2H, d, J=8.6 Hz), 7.61 (1H, d, J=8.0 Hz), 7.07 (2H, d, J=8.9 Hz), 4.35 (2H, q, J=7.5 Hz), 3.83-3.72 (2H, m), 3.54-3.44 (2H, m), 3.26-3.13 (4H, m), 2.80 (3H, s), 2.68 (3H, s), 1.28 (3H, t, J=7.1 Hz).

Examples 119-127

The following compounds were made by the method of Example 118 using the appropriate aniline in step 5. Examples containing secondary amines on the aniline were synthesized using the appropriate Boc protected aminoaniline and in the final step were treated with a solution of hydrogen chloride in an organic solvent to produce the example compound, usually isolated as the hydrochloride salt.

LCMS LCMS Ex. Structure MW Method Ion Rt 119

557.1 C 558 1.80 120

575.1 C 576 1.45 121

522.6 C 524 1.35 122

556.1 C 557 1.41 123

528.0 C 529 1.39 124

546.0 C 546 1.72 125

561.0 C 562 1.39 126

542.0 C 543 1.45 127

560.0 C 560 1.44

Example 128 6-(2-chloro-4-(2-methylthiazol-5-yl)phenyl)-8-ethyl-2-(4-(1-ethylpiperidin-4-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (64)

Step 1: 6-(2-Chloro-4-(2-methylthiazol-5-yl)phenyl)-8-ethyl-2-(methylthio)pyrido[2,3-d]pyrimidin-7(8H)-one (62)

Bromide 10 (20.5 g, 50 mmol), 2-methylthiazole (6.45 g, 65 mmol, 1.3 eq), tetrakis(triphenylphosphine)palladium (2.9 g, 2.5 mmol, 5 mol %) and potassium acetate (7.4 g, 75 mmol, 1.5 eq.) were placed into a vial with magnetic stirrer, containing 150 ml of degased anhydrous dimethylacetamide. The vial was tightly closed and heated at 110° C. with stirring for 24 h. The reaction mixture was filtered, the residue was washed with chloroform and joined organic solutions were evaporated to dryness. The resulting solid was purified by chromatography on silica gel (gradient elution with EtOAc:hexane-20:80 to 50:50) to afford 62 (10.9 g, 51%).

¹H NMR (400 MHz, DMSO-d6) δ ppm: 1.31 (t, J=7.0 Hz, 3H), 2.62 (s, 3H), 2.71 (s, 3H), 4.43 (q, J=7.0 Hz, 2H), 7.42 (d, J=7.8 Hz, 1H), 7.59 (d, J=7.53 Hz, 1H), 7.76 (d, J=1.6 Hz, 1H), 7.96 (s, 1H), 8.09 (s, 1H), 8.90 (s, 1H).

Step 2: 6-(2-Chloro-4-(2-methylthiazol-5-yl)phenyl)-8-ethyl-2-(methylsulfinyl)pyrido[2,3-d]pyrimidin-7(8H)-one (63)

Sulfide 62 (7.7 g, 18 mmol) was dissolved in 50 ml of anhydrous CH₂Cl₂ and cooled to −5° C. A solution of 70% MCPBA (4.90 g, 20 mmol, 1.1 eq) was then added with stirring at rate at which the temperature did not exceed 0° C. The reaction mixture was allowed to warm up to room temperature, and then was stirred for 1 additional hour (TLC-monitoring). The resulting orange solution was washed twice with saturated aqueous NaHCO₃ (50 ml), water (50 ml). The organic layer was separated, dried over Na₂SO₄ and evaporated. The desired compound 63 was obtained after chromatography on silica gel (4.1 g, 51% yield). ¹H NMR (400 MHz, DMSO-d6) δ ppm: 1.31 (t, J=7.0 Hz, 3H), 2.72 (s, 3H), 2.95 (s, 3H), 4.50 (q, J=6.9 Hz, 2H), 7.46 (d, J=8.1 Hz, 1H), 7.63 (d d, J=7.9 Hz, 1.8 Hz, 1H), 7.80 (d, J=1.6 Hz, 1H), 8.12 (s, 1H), 8.16 (s, 1H), 9.27 (s, 1H).

Step 3: 6-(2-chloro-4-(2-methylthiazol-5-yl)phenyl)-8-ethyl-2-(4-(1-ethylpiperidin-4-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one (64)

A mixture of 6-[2-chloro-4-(2-methyl-1,3-thiazol-5-yl)phenyl]-8-ethyl-2-(methylsulfinyl)pyrido[2,3-d]pyrimidin-7(8H)-one 63 (0.25 g, 0.56 mmol) and 4-(1-ethyl-4-piperidyl)aniline (0.14 g, 0.67 mmol) was dissolved in dichloromethane. The solvent was removed under vacuum. The resulting homogenous solid was heated at 100-120 C for 1 h. The crude solid was purified by silica gel column chromatography and washed with diethylether to afford the desired compound 64 (85 mg, 26% yield). LCMS m/z 585 (M+H)+ Rt 1.55 min. ¹H NMR (400 MHz, CDCl3) δ ppm 8.59 (1H, s) 7.84 (1H, s), 7.68-7.59 (4H, m), 7.50-7.40 (3H, m), 7.32-7.24 (2H, m), 4.52 (2H, q, J=7 Hz), 3.19-3.08 (2H, m), 2.76 (3H, s), 2.61-2.43 (3H, m), 2.13-2.00 (2H, m), 1.95-1.77 (4H, m), 1.41 (3H, t, J=6.7 Hz), 1.16 (3H, t, J=7 Hz).

Examples 129-132

The following compounds were made by the method of Example 128 using the appropriate aniline in step 3. Examples containing secondary amines on the aniline were synthesized using the appropriate Boc protected aminoaniline and in the final step were treated with a solution of hydrogen chloride in an organic solvent or trifluoroacetic acid to produce the example compound, as its respective salt, or washed with saturated sodium bicarbonate solution to afford the final compound as a free base.

LCMS LCMS Ex. Structure MW Method Ion Rt 129

557.1 C 558 1.73 130

603.2 C 604 1.89 131

543.1 C 544 1.40 132

575.1 C 575 1.52

Example 133 2-anilino-6-[2-chloro-4-(3-pyridyl)phenyl]-8-ethylpyrido[2,3-d]pyrimidin-7(8H)-one (66)

A mixture of 6-[2-chloro-4-(3-pyridyl)phenyl]-8-ethyl-2-(methylsulfinyl)pyrido[2,3-d]pyrimidin-7(8H)-one 65 (1 g, 2.35 mmol) and aniline (0.58 g, 2.8 mmol) was heated at 120° C. for 1 h. The mixture was cooled and purified by flash chromatography (CHCl₃:MeOH (19:1)) followed by preparative HPLC purification to provide the desired compound 66 (11 mg, 2%). LCMS m/z 454 (M+H)⁺ Rt 1.76 min. ¹H NMR (400 MHz, CDCl₃) δ ppm 8.89 (1H, bs) 8.67 (1H, bs), 8.63 (1H, s), 7.94 (1H, d, J=7 Hz), 7.77-7.70 (3H, m), 7.67 (1H, s), 7.59-7.53 (2H, m), 7.49-7.39 (4H, m), 7.16 (1H, t, J=6.4 Hz), 4.56 (2H, q, J=7 Hz), 1.44 (3H, t, J=7 Hz).

Example 134 Synthesis of 4-(3-chloro-4-(8-methyl-2-(4-(1-methylpiperidin-4-yl)phenylamino)-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl)phenyl)-N-methylpicolinamide (74)

Synthesis of Intermediates Intermediate 75: Synthesis of methyl 2-(2-chloro-4-(2-(methylcarbamoyl)pyridin-4-yl)phenyl)acetate

Step 1: Synthesis of 4-bromo-N-methylpicolinamide (78)

A mixture of 4-bromopicolinic acid (15 g, 75 mmol), methanamine hydrochloride (15 g, 75 mmol), HOBt (10 g, 75 mmol), EDCI (21 g, 75 mmol) and Et₃N (41.5 mL, 300 mmol) in DMF (200 mL) was stirred at r.t. for 18 h. Water was added to the reaction mixture and the resulting mixture was filtered to afford 16 g of 78 as solid which was used directly in the next step. LCMS: m/z 215 (M+1)⁺

Step 2: Synthesis of [2-Chloro-4-(2-methylcarbamoyl-pyridin-4-yl)-phenyl]acetic acid methyl ester (75)

KOAc (19 g, 194 mmol) and Pd(dppf)Cl₂ (5% mol.) were added successively under vigorous stirring to a solution of 4-bromo-N-methylpicolinamide (16 g, 75 mmol) and methyl 2-(2-chloro-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)acetate (25 g, 82 mmol) in toluene (200 ml), THF (200 ml) and water (50 ml) under N₂. The reaction mixture was heated to reflux for 18 h. The resulting mixture was evaporated to dryness and the solid was extracted with DCM (3×50 ml). The combined organic layers were dried by Na₂SO₄, concentrated in vacuo and chromatographed on silica gel eluting with PE:ethyl acetate (1:1) to afford 75 (11 g, 47%) as pale white solid. LCMS: m/z 319 (M+1)⁺.

Intermediate 76: Synthesis of 4-(1-Methyl-piperidin-4-yl)-phenylamine

Step 1: Synthesis of 4-(4-nitrophenyl)pyridine (82)

200 mg of Pd(dppf)Cl₂ was added to a mixture of pyridin-4-ylboronic acid (2.46 g, 20 mmol), 1-bromo-4-nitrobenzene (4.42 g, 22 mmol), and K₂CO₃ (8.28 g, 60 mmol) in dioxane: H₂O (3:1, 40 mL). The mixture was stirred for 18 h under N₂ at 80° C. The solvent was evaporated in vacuo to dryness. The residue was diluted with 50 mL of DCM, washed with water, dried over Na₂SO₄ and evaporated to dryness. The residue was purified by silica gel column chromoatograpy (PE/ethyl acetate, 10:1-3:1) to afford 82 (3.64 g, 91%) as a white solid.

LCMS m/z 201 (M+1)⁺.

Step 2: Synthesis of (83)

A mixture of 4-(4-nitrophenyl)pyridine (1.0 g, 5 mmol) and MeI (3.55 g, 25 mmol) in 10 mL of actone was stirred for 18 h at r.t. The solid that formed was collected by filtration, washed with cold actone, dried in vacuo to afford 83 (1.56 g, 91%) as a pale yellow solid. ¹H NMR (300 MHz, DMSO-d₆) δ ppm 9.14 (d, 2H, J=6.9 Hz), 8.62 (d, 2H, J=6.9 Hz), 8.47 (d, 2H, J=9.0 Hz), 8.33 (d, 2H, J=9.0 Hz), 4.38 (s, 3H).

Step 3: Synthesis of 1-methyl-4-(4-nitro-phenyl)-1,2,3,6-tetrahydro-pyridine (84)

To a suspension of 83 (1.56 g, 4.56 mmol) in 20 ml of MeOH was added NaBH₄ (0.52 g, 13.68 mmol) in portions at 0. The mixture was stirred for 4 h at r.t. The mixture was treated with 40 mL of sat aq NaHCO₃. The solid that formed was collected by filtration and dissolved in 20 mL of 1 N HCl, washed with MTBE (2×20 mL). Then the aqueous phase was diluted with sat aq Na₂CO₃, extracted with DCM (3×30 mL), dried over Na₂SO₄ and evaporated to afford 84 (850 mg, 85%) as a pale yellow solid. LCMS m/z 219 (M+1)⁺.

Step 4: Synthesis of 4-(1-methyl-piperidin-4-yl)-phenylamine (76)

A mixture of 1-methyl-4-(4-nitrophenyl)-1,2,3,6-tetrahydropyridine (850 mg, 3.88 mmol) and 0.4 g of Pd/C in 30 mL of MeOH was placed under 50 psi of H₂ gas for 18 h. The mixture was filtered and the filtrate was evaporated to afford 76 (690 mg, 94%) as a white solid.

LCMS m/z 191 (M+1)⁺. NMR (400 MHz, CDCl3) δ ppm 7.02 (d, 2H, J=8.4 Hz), 6.63 (d, 2H, J=8.4 Hz), 3.56-3.48 (m, 2H), 2.97-2.94 (m, 2H), 3.56 (s, 2H), 2.40-2.30 (m, 1H), 2.31 (s, 3H), 2.10-1.95 (m, 1H), 1.82-1.69 (m, 1H).

Synthesis of 4-(3-chloro-4-(8-methyl-2-(4-(1-methylpiperidin-4-yl)phenylamino)-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl)phenyl)-N-methylpicolinamide (74) Step 1: Synthesis of ethyl 2,4-dioxohexahydropyrimidine-5-carboxylate (68)

Pyridine (5 mL) was added to a mixture of 2,4-dioxohexahydropyrimidine-5-carboxylic acid 1 (50 g, 0.32 mmol) in 250 mL of SOCl₂ at 15-25° C. After the addition was complete, the temperature was raised to 75° C. for 16 h. The mixture was evaporated to afford a pale yellow solid. Dry EtOH (500 mL) was added slowly and then the mixture was stirred at reflux for 16 h. The reaction was cooled to 0° C. under ice batch and then filtered to afford 68 as a white solid (46.3 g, 79%). LCMS m/z 185 (M+1)⁺. ¹H NMR (400 MHz, CDCl₃) δ 8.03 (d, J=6.4 Hz, 1H), 4.18 (q, J=7.2 Hz, 2H), 1.21 (t, J=7.2 Hz, 3H).

Step 2: Ethyl 2,4-dichloropyrimidine-5-carboxylate (69)

To a mixture of ethyl 2,4-dioxohexahydropyrimidine-5-carboxylate 2 (46.3 g, 0.251 mol) in 126 mL of POCl₃ was added N,N-diethylaniline (52.4 g, 0.351 mol). The mixture was stirred at 105° C. overnight. This mixture was cooled to r.t. and poured into ice, filtered to afford pale yellow solid. The solid was dissolved in 100 mL of DCM and dried over Na₂SO₄, filtered and evaporated to afford 41.6 g of 69 as a yellow solid (69%). NMR (300 MHz, CDCl₃) ppm 9.01 (s, 1H), 4.47 (q, J=7.2 Hz, 2H), 1.41 (t, J=7.2 Hz, 3H).

Step 3: Ethyl 2-chloro-4-(methylamino) pyrimidine-5-carboxylate (70)

Methylamine in THF (2N, 19.2 mL) was added dropwise to a solution of ethyl 2,4-dichloropyrimidine-5-carboxylate 3 (8.5 g, 38.4 mmol) and Et₃N (5.36 mL, 38.4 mmol) in 100 mL of dry DCM at −78° C. This mixture was stirred at −78° C. for 3 h. This mixture was washed with water (30 mL). The organic layer was dried over Na₂SO₄, filtered and evaporated to dryness to afford 70 (8.4 g). LCMS: m/z 216 (M+1)⁺.

Step 4: (2-chloro-4-(methylamino) pyrimidin-5-yl)methanol (71)

A solution of ethyl 2-chloro-4-(methylamino) pyrimidine-5-carboxylate 70 (8.3 g, 38.6 mmol) was added dropwise to a mixture of LiAlH₄ (2.2 g, 57.9 mmol) in 100 mL of dry THF at 0-5° C. The mixture was stirred at 0-5° C. for 1 h. This mixture was quenched sequentially with water (132 uL), 1N NaOH (132 uL) and water (132 uL). Then reaction mixture was dried over MgSO₄, filtered and evaporated to dryness to afford crude 71 (5.7 g). LCMS: m/z 174 (M+1)⁺.

Step 5: 2-chloro-4-(methylamino) pyrimidine-5-carbaldehyde (72)

MnO₂ (14.3 g, 164 mmol) was added to a mixture of (2-chloro-4-(methylamino) pyrimidin-5-yl)methanol 71 (5.7 g, 32.8 mmol) in 800 mL of dry THF. The mixture was stirred at 40° C. for 1 h. This final mixture was filtered and evaporated to dryness to afford crude product. The crude material was purified by silica gel column (PE:ethyl acetate=12:1) to afford 72 as a white solid (1.8 g, 32%). ¹H NMR (400 MHz, CDCl₃) δ ppm 9.83 (s, 1H), 8.66 (brs, 1H), 8.41 (s, 1H), 3.15 (d, J=4.8 Hz, 3H).

Step 6: 4-(3-chloro-4-(2-chloro-8-methyl-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl)phenyl)-N-methylpicolinamide (73)

A mixture of 2-chloro-4-(methylamino)pyrimidine-5-carbaldehyde (72) (300 mg, 1.75 mmol), methyl 2-(2-chloro-4-(2-(methylcarbamoyl)pyridin-4-yl)phenyl)acetate 75 (482 mg, 1.75 mmol) and DBU (38 ul 0.15 eq-0.5 eq) was stirred overnight in 5 mL of DMSO. This mixture was cooled to 0° C., water was added, filtered and dried to afford 73 (250 mg). LCMS m/z 440 (M+1)⁺.

Step 7: 4-(3-chloro-4-(2-chloro-8-methyl-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl)phenyl)-N-methylpicolinamide (74)

A mixture of 4-(1-methylpiperidin-4-yl)aniline (65 mg, 0.34 mmol) and TFA (76 ul, 1.02 mmol) in 3 mL of DMSO was stirred for 5 min, then 4-(3-chloro-4-(2-chloro-8-methyl-7-oxo-7,8-dihydropyrido[2,3-d]pyrimidin-6-yl)phenyl)-N-methylpicolinamide (150 mg, 0.34 mmol) was added. The mixture was stirred for 16 h at 110° C. The mixture was purified directly by prep HPLC to afford 74 (79 mg, 37%) and isolated as an HCl salt. LCMS m/z 594 (M+1)⁺. ¹H NMR (400 MHz, DMSO-d₆) δ ppm 10.45 (brs, 1H), 10.24 (brs, 1H), 8.94-8.91 (m, 1H), 8.86 (s, 1H), 8.76 (d, J=5.2 Hz, 1H), 8.38 (d, J=1.2 Hz, 1H), 8.08 (d, J=1.6 Hz, 1H), 8.07 (dd, J=5.2 Hz, 1.6 Hz, 1H), 7.97 (s, 1H), 7.94 (dd, J=8 Hz, 1.6 Hz, 1H), 7.83 (d, J=8.4 Hz, 2H), 7.61 (d, J=8.4 Hz, 1H), 7.26 (d, J=8.4 Hz, 2H), 3.68 (s, 3H), 3.50-3.47 (m, 2H), 3.11-3.04 (m, 2H), 2.88 (d, J=4.8 Hz, 3H), 2.68 (d, J=1.6 Hz, 4H), 1.99-1.98 (m, 4H).

Examples 135-205

The following compounds in Examples 135-205 were made using the method in Example 134. The general route is described below. In the first step, the appropriate aldehyde A is condensed with appropriate phenyl acetate B to afford the chloropyrimidine C. In the final step, the intermediate C is reacted with the appropriate aniline D to afford the final compounds E.

Aniline Intermediates

Aniline intermediates were synthesized using the route outlined in the synthesis of intermediate 74 or were synthesized using one of the procedures in the routes described below.

Intermediate 92: Synthesis of 4-(1-ethylpiperidin-4-yl)-3-fluoroaniline

Step 1: Synthesis of 4-(2-fluoro-4-nitrophenyl)pyridine (86)

A solution of compound 85 (50 g, 227 mmol, 1.05 eq), pyridine-4-ylboronic acid (26.6 g, 216 mmol), Pd(dppf)Cl₂ (11.3 g, 10.8 mmol, 5 mol %) and K₂CO₃ (89.6 g, 649 mmol) in dioxane/H₂O (400 mL, 3:1) was stirred at 90° C. for 18 h under nitrogen. The solution was then concentrated, 200 mL EtOAc was added and filtered and the residue was washed with ethyl acetate (40 ml), then the organic layer was washed with H₂O (4×60 mL). The organic layer was dried over Na₂SO₄, filtered and concentrated to afford 86 as a brown solid, which was used the next step directly without further purification (47.2 g, 95%). LCMS m/z 219 (M+1)⁺.

Step 2: Synthesis of 4-(2-fluoro-4-nitrophenyl)pyridine (86)

To a solution of compound 86 (8 g, 36.7 mmol) in acetone (200 mL) was added CH₃I (15.64 g, 110.1 mmol), which was stirred at r.t. for 16 h. The reaction mixture was filtered and the residue was washed with acetone (20 mL), dried to afford 87 as a yellow solid. (8.5 g, 64%) which was used in the next step without further purification. ¹H NMR (400 MHz, CDCl₃) 8 ppm 9.17 (d, J=5.6 Hz, 2H), 8.47 (d, J=5.6 Hz, 2H), 8.44 (d, J=8 Hz, 1H), 8.33 (d, J=8 Hz, 1H), 8.14 (t, J=8 Hz, 1H), 4.42 (s, 3H).

Step 3: Synthesis of 4-(2-fluoro-4-nitrophenyl)-1-methyl-1,2,3,6-tetrahydropyridine (88)

NaBH₄ (2.56 g, 71.03 mmol, 3.0 eq) was added to a solution of compound 87 (8.5 g, 23.61 mmol) in MeOH (60 ml) at 0° C. over 5 min. This mixture was stirred at r.t. for 4 h. The reaction was quenched with saturated aqueous NH₄Cl, then H₂O (300 mL) was added. The reaction mixture was extracted with DCM (4×20 mL), dried over Na₂SO₄, filtered and concentrated to afford 88 as a dark red oil, which was used the next step directly without further purification (4.5 g, 82%). ¹H NMR (300 MHz, CDCl₃) 8 ppm 7.99 (dd, J=8.4 Hz, 2.1 Hz, 1H), 7.92 (dd, J=7.5 Hz, 2.1 Hz, 1H), 7.42 (t, J=7.5 Hz, 1H), 6.14-6.12 (m, 1H), 3.16-3.13 (m, 2H), 2.69-2.66 (m, 2H), 2.59-2.57 (m, 2H), 2.41 (s, 3H).

Step 4: Synthesis of 3-fluoro-4-(1-methylpiperidin-4-yl)aniline (89)

A mixture of compound 88 (4.5 g, 19.07 mmol) and 10% Pd/C (1 g) in methanol (100 mL) was stirred under 40 psi of H₂ at room temperature for 3 h. The reaction mixture was filtered and the filtrate was concentrated to afford 89 as a pale yellow solid, and used in the next step without further purification (4.5 g, 99%). ¹H NMR (300 MHz, CDCl₃) 8 ppm 6.99 (t, J=8.4 Hz, 1H), 6.42 (dd, J=8.4 Hz, 2.4 Hz, 1H), 6.36 (dd, J=12.3 Hz, 2.4 Hz, 1H), 3.65 (brs, 2H), 2.97-2.93 (m, 2H) 2.73-2.67 (m, 1H), 2.30 (s, 3H), 2.10-2.00 (m, 2H), 1.78-1.72 (m, 4H).

Step 5: Synthesis of Compound (90)

To EtI (15.64 g, 110.1 mmol) was added to a solution of compound 86 (8 g, 36.7 mmol) in CH₃CN (200 mL). The resulting mixture was heated at reflux for 16 h. The reaction mixture was concentrated to dryness, then 100 ml DCM was added, extracted with H₂O (5×20 mL), the combined aqueous layers were extracted with DCM (6×20 mL), dried over Na₂SO₄, filtered and concentrated to afford 90, which was used in the next step without further purification (6.0 g, 44%,). ¹H NMR (400 MHz, CDCl₃) 8 ppm 9.55 (d, J=6.8 Hz, 2H), 8.38 (d, J=6.0 Hz, 2H), 8.29 (dd, J=8.4 Hz, 1.6 Hz, 1H), 8.17 (dd, J=9.6 Hz, 2 Hz, 1H), 8.06 (t, J=8 Hz, 1H) 5.08 (q, J=7.2 Hz, 2H), 1.78 (t, J=7.2 Hz, 3H).

Step 6: Synthesis of ethyl-4-(2-fluoro-4-nitrophenyl)-1,2,3,6-tetrahydropyridine (91)

NaBH₄ (1.74 g, 48.26 mmol, 3.0 eq) was added to a solution of compound 90 (6.0 g, 16.01 mmol) in MeOH (60 mL) at 0° C. over 5 min. The mixture was stirred at r.t. for 4 h. The reaction was quenched with saturated aqueous NH₄Cl, then H₂O (300 mL) was added, and then the reaction mixture was extracted with DCM (4×20 ml). The combined organic layer were dried over Na₂SO₄, filtered and concentrated to afford 91 as a dark red oil, which was used the next step directly without further purification (2.8 g, 70%). LCMS:m/z 251 (M+1)⁺. ¹H NMR (300 MHz, CDCl₃) δ ppm 7.98 (dd, J=8.7 Hz, 1.8 Hz, 1H), 7.91 (dd, J=10.5 Hz, 2.1 Hz, 1H), 7.42 (t, J=8.4 Hz, 1H), 6.16 (brs, 1H), 3.19 (q, J=3 Hz, 2H), 2.72-2.68 (m, 2H), 2.57-2.50 (m, 4H), 1.17 (t, J=7.2 Hz, 3H).

Step 7: Synthesis of 4-(1-ethylpiperidin-4-yl)-3-fluoroaniline (92)

A mixture of compound 91 (2.8 g, 11.2 mmol) and 10% Pd/C (1 g) in methanol (100 mL) was stirred under 40 psi of H₂ at room temperature for 3 h. The mixture was filtered, and the filtrate was concentrated to afford 92 as a pale yellow solid (2.5 g, 89% yield). ¹H NMR (300 MHz, CDCl₃) δ ppm 7.00 (d, J=8.4 Hz, 1H), 6.41 (dd, J=8.4 Hz, 2.4 Hz, 1H), 6.35 (dd, J=12 Hz, 2.4 Hz, 1H), 3.63 (brs, 2H), 3.06-3.02 (m, 2H), 2.78-2.68 (m, 1H), 2.46 (q, J=7.2 Hz, 2H), 2.05-1.96 (m, 2H), 1.79-1.71 (m, 4H), 1.10 (t, J=7.2 Hz, 3H).

Intermediate 102: Synthesis of 4-(octahydroindolizin-7-yl)aniline

Step 1: Synthesis of diethyl 7-oxooctahydroindolizin 6,8-dicarboxylate (95)

Compound 93 (100 g, 0.625 mol), 800 mL of ethanol and 0.15 g (0.45 mmol) of helianthin (methyl orange) were mixed. To the stirred mixture, 225 mL of a dilute hydrochloric acid (2.74 M) was slowly added followed by 45.8 ml (0.625 mol) of a formaldehyde solution, 124.8 g (0.625 mol) of compound 94 and 150 mL of ethanol were added. The mixture was stirred at room temperature for 3 days. The mixture was concentrated in vacuo to ca.500 mL. The mixture was cooled in an ice bath before the addition of 1N NaOH (625 mL) which cause the separation of an oily solid. The aqueous phase was separated and the residue was triturated in ether (250 mL). After 30 min, the precipitate was collected by filtration, washed with ether (2×60 mL), and dried in vacuo to afford 95 as a pale yellow solid (110 g, 62%). LCMS: m/z 284 (M+1)⁺.

Step 2: Synthesis of hexahydroindolizin-7(1H)-one (96)

A solution of 110 g (0.389 mol) of compound 95 in 1000 mL of 6M HCl was refluxed in an oil bath with 1 g of zinc dust for 4 h. The stirred solution was cooled in an ice bath, neutralized by slow addition of aqueous 20% ammonia. The solution was extracted with CHCl₃ (6×60 mL). The organic layers were dried over Na₂SO₄, filtered and concentrated. The residue was distilled under reduced pressure at 110-120° C. to afford 96 as a colorless oil (18 g, 33%). ¹H NMR (400 MHz, CDCl₃) ppm: 1.61-1.49 (m, 1H,), 1.91-1.78 (m, 1H), 2.00-1.97 (m, 2H) 2.39-2.23 (m, 5H) 2.56-2.53 (m, 2H) 3.18-3.17 (m, 1H), 3.35-3.32 (m, 1H).

Step 3: Synthesis of 1,2,3,5,8,8a,-hexahydroindolizin-7-yl-trifluoromethanesulfonate (98)

A LiHMDS (1 M in THF, 155 mL) was added dropwise to a solution of compound 96 (18.0 g, 129 mmol) and compound 5d (51 g, 142.4 mmol) in THF (500 mL) at −78° C. under N₂. The reaction mixture was stirred at −78° C. for 2 h. The reaction was then slowly warmed to room temperature for 20 h. The reaction mixture was quenched with saturated ammonium chloride (4 ml), dilute with ethyl acetate (50 mL) and dried over Na₂SO₄. The mixture was filtered and concentrated to afford 98 as a brown oil (35 g, 100%) which was used the next step without further purification. LCMS: m/z 272 (M+1)⁺.

Step 4: Synthesis of 4-(1,2,3,5,8,8a-hexahydroindolizin-7-yl)aniline (100/101)

A solution of compound 98 (35 g, 129.2 mmol), 99 (33.6 g, 194 mmol), Pd(dppf)Cl₂ (6.7 g, 6.46 mmol, 5 mol %) and Na₂CO₃ (27.4 g, 258.4 mmol) in dioxane/H₂O (400 mL, 3:1) was stirred at 90° C. for 18 h under N₂. The reaction mixture was concentrated to dryness, diluted with DCM (200 mL) and filtered. The residue was washed with DCM (40 mL), then the organic layer was acidified with 1N HCl (60 mL). The mixture was stirred for 15 min at r.t. The aqueous layer was neutralizated with 1N aqueous NaOH, extracted with DCM (3×20 mL). The combined organic layers were dried over Na₂SO₄, filtered and concentrated to afford 100/101 as a mixture of isomers (10 g, 36%). ¹H NMR (300 MHz, CDCl₃) ppm: 7.21 (dd, J=6.6 Hz, 2.1 Hz, 2H), 6.63 (dd, J=6.6 Hz, 2.1 Hz, 2H), 5.94-5.93 (m, 1H) 3.68-3.61 (m, 3H) 3.22-3.19 (td, J=8.4 Hz, 2.4 Hz, 1H) 2.91-2.86 (m, 1H), 2.64-2.59 (m, 1H), 2.32-2.24 (m, 2H), 2.22-2.16 (m, 1H), 2.13-2.02 (m, 1H), 1.90-1.76 (m, 2H), 1.55-1.52 (m, 1H).

Step 5: Synthesis of 4-(octahydroindolizin-7-yl)aniline (102)

A mixture of compounds 100/101 (5 g, 23.15 mmol) and 10% Pd/C (1 g) in methanol (100 mL) was stirred under 40 psi of H₂ at room temperature for 16 h. The residue was filtered and the filtrate was concentrated to afford 102 as a pale yellow solid (5 g, 100%). LCMS m/z 217 (M+1)⁺.

Intermediates 106 and 109: Synthesis of tert-butyl 4-(4-aminophenyl)piperidine-1-carboxylate (106) and 4-(1-isopropylpiperidin-4-yl)aniline (109)

Step 1: Synthesis of 4-trifluoromethanesulfonyloxy-3,6-dihydro-2H-pyridine-1-carboxylic acid tert-butyl ester (104)

LiHMDS (1M, 301 mL, 0.301 mol) was added dropwise slowly to a solution of 4-oxo-piperidine-1-carboxylic acid tert-butyl ester (50 g, 0.251 mol), and n,n-bis(trifluoromethylsulfonyl) aniline (88.72 g, 0.276 mol) in THF (dry, 1 L) under N₂ at −78° C. over 1 hr. The mixture was stirred at −78° C. for 2 hr, and then warmed to r.t. slowly over 16 hr. The reaction mixture was quenched with saturated aqueous NH₄Cl solution (1 L), keeping the temperature below 30° C. The mixture was stirred at r.t. for 20 min, and then the organic layer was separated. The aqueous layer was extracted with MTBE (2×300 mL). All of the organic layers were combined, washed with brine (2×1 L), dried over Na₂SO₄, filtered and concentrated under reduced pressure at low temperature below 40° C. to afford the desired crude product 104 (84 g). LCMS m/z 276.0 (M−55)⁺.

Step 2: Synthesis of 4-(4-amino-phenyl)-3,6-dihydro-2H-pyridine-1-carboxylic acid tert-butyl ester (105)

To a solution of 4-trifluoromethanesulfonyloxy-3,6-dihydro-2H-pyridine-1-carboxylic acid tert-butyl ester 104 (84 g, 0.251 mol) in dioxane/H₂O (v:v=3:1, 1000 mL) was added 4-aminophenylboronic acid hydrochloride (46 g, 0.266 mol), K₂CO₃(103 g, 0.753 mol), and Pd(dppf)Cl₂(12 g) under N₂. The mixture was stirred at 80-90° C. under N₂ for 16 hr. The mixture was cooled to r.t., filtered, and the solid was washed with ethyl acetate (300 mL). All of the filtrate was combined, diluted with water (500 mL) and concentrated under reduced pressure and followed by extraction with DCM (5×300 mL). The organic layers were combined, dried over Na₂SO₄, filtered and concentrated. The crude material was purified by silica gel column chromatography (PE/EtOAc=20:1→10:1) to afford 35 g of compound 105. (51%) LCMS m/z 275 (M+1)⁺.

Step 3: Synthesis of 4-(4-benzyloxycarbonylamino-phenyl)-3,6-dihydro-2H-pyridine-1-carboxylic acid tert-butyl ester (106)

To a solution of 4-(4-amino-phenyl)-3,6-dihydro-2H-pyridine-1-carboxylic acid tert-butyl ester (10 g, 36.5 mmol) in toluene (200 mL) was added NaOH (2.2 g, 54.7 mmol) in water (100 mL). The mixture was cooled to 0° C. and treated with CbzCl (6.2 g, 36.5 mmol) dropwise. The mixture was stirred at r.t. for 16 hr. The organic layer was separated, and the aqueous layer was extracted with EtOAc (3×100 mL). The organic layers were combined and dried over Na₂SO₄, filtered and concentrated to afford the desired compound 106 (14.89 g, 96%). LCMS m/z 309 (M−99)⁺.

Step 4: Synthesis of [4-(1,2,3,6-tetrahydro-pyridin-4-yl)-phenyl]-carbamic acid benzyl ester (107)

A solution of 4-(4-benzyloxycarbonylamino-phenyl)-3,6-dihydro-2H-pyridine-1-carboxylic acid tert-butyl ester (28.7 g, 0.07 mmol) in TFA/DCM (v:v=1:4, 500 mL) was stirred at r.t. for 3 hr. The mixture was cooled to 0° C., and the pH was adjusted to pH 8-9 with 5 M NaOH. The mixture was extracted with twice with DCM/MeOH (v:v=10:1, 200 mL). The organic layers were combined and dried over Na₂SO₄, filtered and concentrated to afford the desired compound 107 (21.6 g, 93%). LCMS m/z 309 (M+1)⁺.

Step 5: Synthesis of [4-(1-isopropyl-1,2,3,6-tetrahydro-pyridin-4-yl)-phenyl]-carbamic acid benzyl ester (108)

To a solution of [4-(1,2,3,6-tetrahydro-pyridin-4-yl)-phenyl]-carbamic acid benzyl ester (4.5 g, 14.46 mmol), K₂CO₃(3 g, 21.9 mmol) in MeCN (50 mL) was added 2-iodo-propane (2.5 g, 14.46 mmol). The mixture was heated to 50° C. for 16 hr, then cooled to r.t., filtered and concentrated to afford a crude product. The compound was purified by silica gel column chromatography (PE/EtOAc=10:1→1:1) to afford the desired compound 108 (2.5 g, 50%).

LCMS m/z 351 (M+1)⁺.

Step 6: Synthesis of 4-(1-isopropyl-piperidin-4-yl)-phenylamine (109)

To a solution of [4-(1-isopropyl-1,2,3,6-tetrahydro-pyridin-4-yl)-phenyl]-carbamic acid benzyl ester (2.5 g, 7.14 mmol) in MeOH (200 mL) was added Pd/C (0.5 g). The mixture was stirred at r.t. under an average pressure of 40 psi of H₂ for 16 hr. The mixture was filtered and concentrated to afford the desired compound 109 (1.4 g, 90%). LCMS: m/z 219 (M+1)⁺.

Intermediate 112: Synthesis of 4-(2-(dimethylamino)ethyl)aniline

Step 1: Synthesis of N,N-dimethyl-2-(4-nitrophenyl)ethanamine (111)

A mixture of 1-(2-bromoethyl)-4-nitrobenzene (9.2 g, 40 mmol), dimethylamine hydrochloride (6.52 g, 80 mmol) and K₂CO₃ (11 g, 2 eq) was refluxed in 100 mL of MeOH for 3 h. This mixture was filtered and the filtrate was evaporated. The crude product was purified by silica gel column (PE:ethyl acetate=1:1) to afford 5.5 g of target 111 as yellow oil (71%). The compound was used in the next reaction without further purification. ¹H NMR (300 MHz, CDCl₃) ppm: 8.14 (dd, J=9.3 Hz, 2.4 Hz, 2H), 7.36 (dd, J=9.3 Hz, 2.4 Hz, 2H), 2.87 (t, J=6 Hz, 2H), 2.56 (t, J=6 Hz, 2H), 2.28 (s, 6H).

Step 2: Synthesis of 4-(2-(dimethylamino)ethyl)aniline (112)

A mixture of N,N-dimethyl-2-(4-nitrophenyl)ethanamine 111 (5.5 g, 0.028 mol) and 1 g of Pd/C was stirred in 500 mL of MeOH under H₂ at 45 psi for 16 h. This mixture was filtered to afford 112 as white solid (4 g, 86%). ¹H NMR (300 MHz, CDCl₃) ppm: 7.00 (d, J=8.4 Hz, 2H), 6.63 (d, J=8.4 Hz, 2H), 3.55 (m, 2H), 2.69-2.64 (m, 2H), 2.49-2.43 (m, 2H) 2.27 (s, 6H).

Intermediate 115: Synthesis of 4-(2-(pyrrolidin-1-yl)ethyl)aniline

Step 1: Synthesis of 1-(4-nitrophenethyl)pyrrolidine (114)

A mixture of 1-(2-bromoethyl)-4-nitrobenzene (2 g, 8.69 mmol) and pyrrolidine (1.85 g, 26.08 mmol) was heated at reflux in 20 mL of MeOH for 3 h. This mixture was filtered and evaporated. The crude product was purified by silica gel column chromatography (PE:ethyl acetate=1:1) to afford 1.9 g of 114 as a yellow oil (99%). ¹H NMR (400 MHz, CDCl₃) ppm: 8.16 (dd, J=24.8 Hz, 8.4 Hz, 2H), 7.38 (dd, J=24.8 Hz, 8.4 Hz, 2H), 2.95-2.91 (m, 2H), 2.75-2.71 (m, 2H), 2.58-2.50 (m, 4H), 1.85-1.79 (m, 4H).

Step 2: Synthesis of 4-(2-(pyrrolidin-1-yl)ethyl)aniline (115)

A mixture of 1-(4-nitrophenethyl)pyrrolidine 114 (1.9 g, 8.64 mmol) and 500 mg of Pd/C was stirred in 100 mL of MeOH under H₂ at 45 psi for 16 h. This mixture was filtered to afford 115 as a white solid (1.6 g, 98%). ¹H NMR (400 MHz, CDCl₃) ppm: 7.07 (dd, J=15.6 Hz, 8.4 Hz, 2H), 6.67 (dd, J=15.6 Hz, 8.4 Hz, 2H), 3.58 (brs, 2H), 2.77-2.71 (m, 2H), 2.69-2.60 (m, 2H), 2.59-2.56 (m, 4H), 1.867-1.80 (m, 4H).

Intermediate 118: Synthesis of 4-(2-morpholinoethyl)aniline

Step 1: Synthesis of 4-(4-nitrophenethyl)morpholine (117)

A mixture of 1-(2-bromoethyl)-4-nitrobenzene (2 g, 8.69 mmol) and morpholine (2.27 g, 26.08 mmol) was refluxed in 20 mL of MeOH for 18 h. This mixture was filtered and evaporated. The crude product was purified by silica gel column chromatography (PE:ethyl acetate=1:1) to afford 117 as yellow oil (2 g, 98%). ¹H NMR (400 MHz, CDCl₃) ppm: 8.18 (d, J=8.8 Hz, 2H), 7.40 (d, J=8.8 Hz, 2H), 3.76-3.72 (m, 4H), 2.94-2.91 (m, 2H), 2.71-2.63 (m, 2H), 2.55-2.46 (m, 4H).

Step 2: Synthesis of 4-(2-morpholinoethyl)aniline (118)

A mixture of 4-(4-nitrophenethyl)morpholine 117 (2 g, 8.46 mmol) and 500 mg of Pd/C was stirred in 100 mL of MeOH under H₂ at 45 psi for 16 h. This mixture was filtered to afford 118 as a white solid (1.9 g, quant. yield). ¹H NMR (300 MHz, CDCl₃) ppm: 6.99 (d, J=9 Hz, 2H), 6.62 (d, J=9 Hz, 2H), 3.73 (m, 4H), 3.60 (brs, 2H), 2.70-2.65 (m, 2H), 2.54-2.47 (m, 6H).

Additional Example of a Phenyl Acetate Derivative:

Phenylacetate analogs were synthesized using the method outlined for the synthesis of intermediate 75. An additional example is outlined in the example of intermediate 75e. Intermediate 75e: Synthesis of methyl 2-(2-fluoro-4-(2-methylpyridin-3-yl) phenyl)acetate

Step 1: Synthesis of 4-bromo-1-(bromomethyl)-2-fluorobenzene (75b)

To a solution of 4-bromo-2-fluoro-1-methylbenzene (6 g, 31.75 mmol) in CCl₄ (50 mL) was added NBS (6.22 g, 34.94 mmol) and BPO (384 mg, 1.59 mmol) under nitrogen, the reaction mixture was stirred at 80° C. for 15 h. The mixture was washed with water, extracted with DCM (2×50 mL). The combined layers were washed with brine (100 mL), dried over Na₂SO₄ and concentrated to afford 75b (9 g) which was used for the next step without further purification. LCMS m/z 269 (M+1)⁺.

Step 2: Synthesis of 2-(4-bromo-2-fluorophenyl)acetonitrile (75c)

To a solution of 4-bromo-1-(bromomethyl)-2-fluorobenzene (9 g, crude) in DCM (50 mL) and H₂O (50 mL) was added KCN (6.56 g, 100.74 mmol) and TBAB (1 g) and stirred at r.t. for 15 h. The mixture was washed with water, extracted with DCM (2×50 mL). The combined layers were washed with brine (100 mL), dried over Na₂SO₄ and concentrated to afford 75c (7 g) which was used in the next step without further purification. LCMS m/z 214 (M+1)⁺.

Step 3: Synthesis of methyl 2-(4-bromo-2-fluorophenyl)acetate (75d)

To a solution of 2-(4-bromo-2-fluorophenyl) acetonitrile (7 g, crude) in MeOH (50 mL) was added dropwise SOCl₂ (35 mL) at 0° C. The mixture was stirred at r.t. for 15 h. The solvent was removed. The residue was washed with water and extracted with EtOAc (3×50 mL). The combined layers were washed with brine (50 mL), dried over Na₂SO₄ and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-10% EtOAc in petroleum ether to afford the desired product (5 g, 68%). LCMS m/z 247 (M+1)⁺.

Step 4: Synthesis of methyl 2-(2-fluoro-4-(2-methylpyridin-3-yl) phenyl)acetate (75e)

To a solution of methyl 2-(4-bromo-2-fluorophenyl)acetate (1 g, 4.05 mmol) in toluene/THF/H₂O (15 mL, v/v/v=2/2/1) were added 2-methylpyridin-3-ylboronic acid (870 mg, 3.97 mmol), AcOK (790 mg, 8.05 mmol) and PdCl₂(dppf) (222 mg, 0.31 mmol) under nitrogen. The reaction mixture was stirred at 90° C. for 15 h. The reaction mixture was filtered, the filtrate was washed with water, extracted with EtOAc (2×10 mL). The combined layers were washed with brine, dried over Na₂SO₄ and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-10% EtOAc in petroleum ether to afford the desired product (0.9 g, 86%). LCMS m/z 260 (M+1)⁺.

Additional Aldehyde Intermediate Intermediate 121: Synthesis of 2-chloro-4-(ethylamino)pyrimidine-5-carbaldehyde

Step 1: Synthesis of ethyl 2-chloro-4-(ethylamino)pyrimidine-5-carboxylate (119)

To ethylamine (10.2 g, 0.226 mol) was added dropwise to a solution of ethyl 2,4-dichloropyrimidine-5-carboxylate (50 g, 0.226 mol) and Et₃N (22.9 g, 0.226 mol) in DCM (500 mL) at −78° C. The reaction was stirred at −78° C. for 3 h, and then warmed up to −30° C. until ethyl 2,4-dichloropyrimidine-5-carboxylate was consumed. The organic layer was washed with water, dried over Na₂SO₄, and concentrated to afford 119 as a white solid (50 g). The compound was used in the next step without further purification. LCMS m/z 230 (M+1)⁺.

Step 2: Synthesis of (2-chloro-4-(ethylamino)pyrimidin-5-yl)methanol (120)

A suspension of LiAlH₄ (12.39 g, 0.326 mol) in anhydrous THF (400 mL) was cooled to 0° C. To the above suspension was added dropwise a solution of ethyl 2-chloro-4-(ethylamino)pyrimidine-5-carboxylate (50 g) in anhydrous THF (100 mL) while keeping the temperature below 10° C. The reaction was stirred at 5-10° C. for 2 h, then quenched with water. The mixture was filtered, and the filtrate was concentrated to afford 120 as a white solid (35 g). The compound was used in the next step without further purification. LCMS m/z 188 (M+1)⁺.

Step 3: Synthesis of 2-chloro-4-(ethylamino)pyrimidine-5-carbaldehyde (121)

MnO₂ (175 g) was added to a solution of (2-chloro-4-(ethylamino)pyrimidin-5-yl)methanol (35 g) in THF (400 mL). The mixture was stirred at 40° C. for 3 h. The mixture was filtered, and the filtrate was concentrated to and then purified by column chromatography on silica gel (PE:ethyl acetate=10:1) to afford 121 (22 g). LCMS m/z 186 (M+1)⁺.

Examples 135-217

The compounds outlined in the table below are synthesized using the method in Example 134 using the appropriate aniline D, appropriate aldehyde A and appropriate phenylacetate intermediate B.

LCMS LCMS Ex. Structure MW Method Ion Rt 135

579.2 D 579 0.90 136

569.1 E 569 1.01 137

593.2 E 593 2.17 138

595.1 E 595 3.06 139

563.1 E 563 1.97 140

567.1 E 567 2.94 141

582.1 E 584 2.60 142

622.2 E 624 0.99 143

591.2 E 591 2.01 144

611.2 E 307 3.26 145

593.2 E 594 1.92 146

597.1 E 597 3.18 147

539.1 E 539 2.02 148

557.1 E 557 3.11 149

597.1 F 597 3.32 150

539.1 E 539 1.77 151

543.0 E 543 2.75 152

597.1 E 579 3.11 153

583.1 E 583 2.96 154

583.1 E 583 3.07 155

583.1 E 585 2.09 156

587.1 E 587 3.03 157

583.1 D 583 2.37 158

597.1 E 599 2.18 159

597.1 E 598 2.12 160

583.1 E 583 3.04 161

601.1 E 601 3.11 162

615.1 E 615 3.31 163

583.1 E 583 2.09 164

551.1 E 552 1.81 165

597.1 E 599 2.16 166

601.1 E 601 3.24 167

601.1 E 601 3.17 168

634.2 E 635 2.85 169

525.1 E 263 1.61 170

591.2 D 296 2.45 171

591.2 D 296 2.43 172

565.1 D 565 2.17 173

594.1 D 596 2.96 174

568.1 D 568 2.86 175

555.1 E 555 3.33 176

529 E 529 2.15 177

565.1 D 565 2.19 178

585.1 D 585 3.15 179

530.7 E 531 2.05 180

569.1 E 569 3.51 181

581.1 D 581 2.08 182

567.1 D 567 2.34 183

571.1 D 571 3.36 184

548.7 E 549 1.79 185

552.1 E 552 3.23 186

566.1 G 567 2.84 187

566.1 G 566 2.68 188

566.1 H 568 2.85 189

569.1 J 285 2.98 190

566.1 J 567 3.06 191

620.1 G 620 3.54 192

607.2 G 608 2.33 193

595.2 H 595 2.08 194

554.1 J 278 2.04 195

540.1 H 270 2.60 196

540.1 H 270 2.48 197

552.1 G 552 2.69 198

468.9 G 469 4.21 199

531.7 G 532 2.61 200

545.7 G 546 2.73 201

538.1 G 538 2.66 202

502.6 G 503 1.93 203

503.6 G 504 2.60 204

517.6 G 519 2.60 205

537.1 G 269 2.02 206

608.1 D 608 0.98 207

537.1 G 269 2.02 208

580.1 G 582 2.94 209

544.7 G 545 2.26 210

594.1 G 594 2.15 211

580.1 G 581 2.16 212

516.7 G 518 1.93 213

608.1 G 608 2.61 214

537.1 G 538 2.27 215

581.1 G 582 2.11 216

594.1 G 595 2.44 217

608.1 G 609 2.59

Examples 218-235

The compounds outlined below are synthesized using the general method in Example 134 using the appropriate aniline D, appropriate aldehyde A and appropriate phenylacetic acetate intermediate B. Some examples of phenyl acetate intermediates are outlined below.

Intermediate 125: Synthesis of methyl 2-(2-chloro-4-(5-methyl-1,2,4-oxadiazol-3-yl)phenyl)acetate

Step 1: Synthesis of methyl 2-(2-chloro-4-cyanophenyl)acetate (123)

To a solution of methyl 2-(4-bromo-2-chlorophenyl)acetate (20 g, 75.90 mmol) in dioxane (250 mL) was added Zn(CN)₂ (6.68 g, 56.89 mmol) and Pd(PPh₃)₄ (4.39 g, 3.80 mmol) under nitrogen. The reaction mixture was stirred at 80° C. for 15 h. The mixture was filtered, and the filtrate was washed with water, extracted with EtOAc (2×100 mL). The combined layers were washed with brine (100 mL), dried over Na₂SO₄ and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-5% EtOAc in petroleum ether to afford 123 (13 g, 82%). LCMS m/z 210 (M+1)⁺.

Step 2: Synthesis of methyl 2-(2-chloro-4-(N-hydroxycarbamimidoyl)phenyl)acetate (124)

To a solution of methyl 2-(2-chloro-4-cyanophenyl)acetate (10 g, 47.70 mmol) in MeOH (150 mL) was added NH₂OH.HCl (6.63 g, 95.41 mmol) and NaHCO₃ (12 g, 142.86 mmol) under nitrogen. The reaction mixture was stirred at 80° C. for 2 h. The solvent was removed, the residue was washed with water and extracted with EtOAc (3×50 mL). The combined layers were washed with brine (50 mL), dried over Na₂SO₄ and concentrated to afford 124 (7 g) which was used for the next step without further purification. LCMS m/z 243 (M+1)⁺.

Step 3: Synthesis of methyl 2-(2-chloro-4-(5-methyl-1,2,4-oxadiazol-3-yl)phenyl)acetate (125)

A solution of methyl 2-(2-chloro-4-(N-hydroxycarbamimidoyl)phenyl)acetate (7 g, 28.85 mmol) in Ac₂O (50 mL) was stirred at 100° C. for 15 h. The reaction mixture was concentrated, dissolved in EtOAc and washed with water. The aqueous was further extracted with EtOAc (2×50 mL). The combined layers were washed with brine (50 mL), dried over Na₂SO₄ and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-10% EtOAc in petroleum ether to afford the desired product 125 (5.7 g, 74%).

LCMS m/z 267 (M+1)⁺.

Intermediate 129: Synthesis of methyl 2-(2-fluoro-4-(5-methyl-1,2,4-oxadiazol-3-yl)phenyl)acetate

Step 1: Synthesis of methyl 2-(4-cyano-2-fluorophenyl)acetate (127)

To a solution of methyl 2-(4-bromo-2-fluorophenyl)acetate (4 g, 16.19 mmol) in dioxane (50 mL) was added Zn(CN)₂ (1.90 g, 16.18 mmol) and Pd(PPh₃)₄ (935 mg, 0.81 mmol) under nitrogen. The reaction mixture was stirred at 80° C. for 15 h. The mixture was filtered, the filtrate was washed with water and extracted with EtOAc (2×50 mL). The combined layers were washed with brine (50 mL), dried over Na₂SO₄ and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-5% EtOAc in petroleum ether to afford the desired product 127 (1.5 g, 48%). LCMS m/z 194 (M+1)⁺.

Step 2: Synthesis of methyl 2-(2-fluoro-4-(N-hydroxycarbamimidoyl)phenyl)acetate (128)

To a solution of methyl 2-(4-cyano-2-fluorophenyl)acetate (1.5 g, 7.77 mmol) in MeOH (15 mL) were added NH₂OH.HCl (1.10 g, 15.83 mmol) and NaHCO₃ (2.0 g, 23.81 mmol) under nitrogen. The reaction mixture was stirred at 80° C. for 2 h. The solvent was removed, the residue was washed with water, extracted with EtOAc (3×20 mL). The combined layers were washed with brine (50 mL), dried over Na₂SO₄ and concentrated to afford the desired product (1.5 g) which was used for the next step without further purification. LCMS m/z 227 (M+1)⁺.

Step 3: Synthesis of methyl 2-(2-fluoro-4-(5-methyl-1,2,4-oxadiazol-3-yl)phenyl)acetate (129)

A solution of methyl 2-(2-fluoro-4-(N-hydroxycarbamimidoyl)phenyl)acetate (1.5 g, 6.63 mmol) in Ac₂O (20 mL) was stirred at 100° C. for 15 h. The mixture was concentrated to dryness. The residue was washed with water, extracted with EtOAc (2×20 mL). The combined layers were washed with brine (30 mL), dried over Na₂SO₄ and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-5% EtOAc in petroleum ether to afford the desired product (1.4 g, 84%). LCMS m/z 251 (M+1)⁺.

Intermediate 138: Synthesis of [2-methyl-4-(5-methyl-[1,2,4]oxadiazol-3-yl)-phenyl]-acetic acid methyl ester

Step 1: Synthesis of 4-bromo-2-methyl-benzoic acid methyl ester (131)

4-bromo-2-methylbenzoic acid 1 h (10 g) was added to SOCl₂ (20 mL). The mixture was stirred at refluxing for 1 h, concentrated and MeOH (20 mL) was added at 0° C. The solution was concentrated. The residue was dissolved, in DCM and washed with water. The organic layer was separated, dried over Na₂SO₄, and concentrated under reduced pressure to afford compound 131 as a light-orange oil (10.7 g). The compound was used in the next reaction without further purification. ¹H NMR (400 MHz, CDCl₃) ppm: 7.79 (d, J=8.4 Hz, 1H), 7.41 (d, J=1.6 Hz, 7.39 (dd, J=8.4 Hz, 1.6 Hz, 1H), 3.88 (s, 3H).

Step 2: Synthesis of (4-bromo-2-methyl-phenyl)-methanol (132)

To a solution of methyl 4-bromo-2-methylbenzoate 131 (10.64 g) in DCM was added LiAlH₄ (3.8 g) at 0° C. The reaction mixture was stirred for 16 h at room temperature. Water (40 mL) was added and extracted with DCM. The organic layer were separated, dried over Na₂SO₄, and concentrated under reduced pressure to give (4-bromo-2-methyl-phenyl)-methanol 132 as a light-orange oil (3.9 g). The compound was used in the next reaction without further purification. LCMS m/z 183 (M−17)⁺.

Step 3: Synthesis of 4-bromo-1-bromomethyl-2-methyl-benzene (133)

To a solution of (4-bromo-2-methylphenyl)methanol 132 (4 g) in DCM was added PBr₃ (5.42 g) at 0° C. The mixture was stirred for 3 h at room temperature. DCM was added and washed with water and aq NaHCO₃ until pH ˜7. The organic layer was separated, dried over Na₂SO₄, and concentrated under reduced pressure to give 4-bromo-1-(bromomethyl)-2-methylbenzene 133 as a brown oil (3.8 g). The compound was used in the next reaction without further purification. ¹H NMR (400 MHz, CDCl₃) ppm: 7.37 (s, 1H), 7.34 (d, J=8 Hz, 1H), 7.20 (d, J=8 Hz, 1H), 4.48 (s, 2H), 2.41 (s, 3H).

Step 4: Synthesis of (4-bromo-2-methyl-phenyl)-acetonitrile (134)

To a solution of 4-bromo-1-(bromomethyl)-2-methylbenzene 133 (3.8) in a mixture of DCM and water was added TBAB and KCN (2.94 g) at 0° C. The mixture was stirred for 16 h at room temperature. DCM was added and the mixture was washed with water and saturated aq NaHCO₃. The organic layer was separated, dried over Na₂SO₄, and concentrated under reduced pressure to afford 2-(4-bromo-2-methylphenyl)acetonitrile 134 as a brown solid (2.9 g). The compound was used in the next reaction without further purification. LCMS: m/z 210 (M+1)⁺.

Step 5: Synthesis of (4-bromo-2-methyl-phenyl)-acetic acid methyl ester (135)

To a solution of 2-(4-bromo-2-methylphenyl)acetonitrile 134 (2.9 g) in MeOH was added SOCl₂ (5 mL) at 0° C. The mixture was stirred for 16 h at room temperature. DCM was added and washed with water, aq NaHCO₃ until pH ˜7. The organic layer was separated, dried over Na₂SO₄, and concentrated under reduced pressure to afford (4-bromo-2-methyl-phenyl)-acetic acid methyl ester 135 as a brown oil (1.9 g, 58%). The compound was used in the next reaction without further purification. LCMS: m/z 243 (M+1)⁺.

Step 6: Synthesis of (4-cyano-2-methyl-phenyl)-acetic acid methyl ester (136)

To a solution of (4-bromo-2-methyl-phenyl)-acetic acid methyl ester 135 (3.0) in 1, 4-dioxane was added ZnCN (1.73 g) and Pd(PPh₃)₄ (2.89 g) under N₂. The mixture was stirred for 16 h at 100° C. After concentration, the compound was purified by silica gel column chromatography to afford (4-cyano-2-methyl-phenyl)-acetic acid methyl ester 136 (1.39 g, 60%). LCMS: m/z 190 (M+1)⁺.

Step 7: Synthesis of [4-(N-hydroxycarbamimidoyl)-2-methyl-phenyl]-acetic acid methyl ester (137)

To a solution of (4-cyano-2-methyl-phenyl)-acetic acid methyl ester 136 (1.39 g) and NaHCO₃ in EtOH was added NH₂OH.HCl (1.85 g). The mixture was refluxed for 2 h. The mixture was concentrated and the residue was dissolved in DCM, washed with water. The organic layer was separated, dried over Na₂SO₄, and concentrated under reduced pressure to afford [4-(N-hydroxycarbamimidoyl)-2-methyl-phenyl]-acetic acid methyl ester 137 as a brown solid (1.45 g, 89%). The compound was used in the next reaction without further purification. ¹H NMR (400 MHz, CDCl₃) ppm: 8.50 (brs, 1H), 7.45 (s, 1H), 7.43 (d, J=8 Hz, 1H), 7.23 (d, J=8 Hz, 1H), 3.69 (s, 3H), 3.66 (s, 2H), 2.33 (s, 3H).

Step 8: Synthesis of [2-methyl-4-(5-methyl-[1,2,4]oxadiazol-3-yl)-phenyl]-acetic acid methyl ester (138)

[4-(N-hydroxycarbamimidoyl)-2-methyl-phenyl]-acetic acid methyl ester 137 (1.45 g) was dissolved in Ac₂O (5 mL). The solution was refluxed for 12 h. The mixture was evaporated and the residue was dissolved in DCM, washed with water. The organic layer was separated, dried over Na₂SO₄, and concentrated under reduced pressure to afford [2-methyl-4-(5-methyl-[1,2,4]oxadiazol-3-yl)-phenyl]-acetic acid methyl ester 138 as a white solid (1.27 g, 79%).

LCMS: m/z 247 (M+1)⁺.

Examples 218-235

LCMS LCMS Ex. Structure MW Method Ion Rt 218

554.1 E 554 2.82 219

588.1 E 588 2.96 220

584.1 E 584 2.95 221

574.1 E 574 2.93 222

542 D 542 3.02 223

556.1 D 556 3.09 224

516 D 516 2.88 225

556.1 E 556 3.38 226

521.6 D 522 3.07 227

572.1 E 572 3.28 228

535.7 F 536 2.94 229

521.6 G 522 2.73 230

539.6 D 540 3.09 232

570.1 D 571 2.84 233

530.0 D 531 2.64 234

582.1 D 583 3.31 235

558.1 D 559 3.27

Examples 236-241

The compounds outlined below are synthesized using the general method in Example 134 using the appropriate aniline D, appropriate aldehyde A and appropriate phenylacetic acetate intermediate B. Some examples of phenyl acetate intermediates are outlined below.

Intermediate 143: Synthesis of (2′-methyl-[2,3′]bipyridinyl-5-yl)-acetic acid methyl ester

Step 1: Synthesis of 2-bromo-5-bromomethyl-pyridine (140)

To a solution of 2-bromo-5-methyl-pyridine (20 g, 116.96 mmol) in CCl₄ (200 mL) was added NBS (21.56 g, 122.80 mmol) and BPO (0.4 g, 1%) under N₂. The mixture was stirred at 80° C. for 16 hr, then cooled to r.t., filtered and concentrated to afford the desired crude compound which was used directly in the next step. LCMS: m/z 252 (M+1)⁺.

Step 2: Synthesis of (6-bromo-pyridin-3-yl)-acetonitrile (141)

To a mixture of 2-bromo-5-bromomethyl-pyridine 140 (29.4 g, 116.96 mmol) and KCN (22 g, 350.88 mmol) in DCM/water (v:v=1:2, 300 mL) was added TBAB (3.77 g, 11.7 mmol). The mixture was stirred at r.t. for 16 hr. The mixture was diluted with DCM (100 mL) and the layers were separated. The aqueous layer was extracted with DCM (3×200 mL), The organic layers were combined, washed with brine (2×200 mL), dried over Na₂SO₄, filtered and concentrated to afford the crude product. The crude product was purified by silica gel column chromatography (PE/EtOAc=10:1→5:1) to afford the desired compound 141 (12.2 g, 53%).

LCMS: m/z 199 (M+1)⁺.

Step 3: Synthesis of (6-bromo-pyridin-3-yl)-acetic acid methyl ester (142)

To a solution of (6-bromo-pyridin-3-yl)-acetonitrile 141 (12.2 g, 62.24 mmol) in MeOH (50 mL) was added dropwise SOCl₂ (11.2 mL, 155.6 mmol) over 10 min at r.t. The mixture was stirred at r.t. for 16 hr, then concentrated to dryness, diluted with water (150 mL), extracted with DCM (3×200 mL). The organic layer was dried over Na₂SO₄, filtered and concentrated to afford the crude product. The crude product was purified by column chromatography with silica gel (PE/EtOAc=10:1→5:1) to afford the desired compound (12 g, 84%). LCMS: m/z 232 (M+1)⁺.

Step 4: Synthesis of (2′-methyl-[2,3′]bipyridinyl-5-yl)-acetic acid methyl ester (143)

To a mixture of (6-bromo-pyridin-3-yl)-acetic acid methyl ester 142 (500 mg 2.17 mmol), 2-Methyl-3-(4,4,5,5-tetramethyl-[1,3,2]dioxaborolan-2-yl)-pyridine (570 mg, 2.60 mmol) and Cs₂CO₃ (2.12 g, 6.51 mmol) in Toluene/THF/water (v:v:v=2:2:1, 10 mL) was added Pd(dppf)Cl₂ (100 mg) under N₂. The mixture was stirred at 80° C. for 3 hr, then cooled to r.t. and filtered. The filtrate was diluted with water (10 mL) and extracted with EtOAc (3×20 mL). The organic layer was dried over Na₂SO₄, filtered and concentrated to afford the crude product, which was purified by column chromatography with silica gel (PE/EtOAc=10:1→5:1→2:1) to afford the desired compound 143 (306 mg, 76%). LCMS: m/z 243 (M+1)⁺.

Intermediate 149: Synthesis of methyl 2-(2′-methyl-3,3′-bipyridin-6-yl)acetate

Step 1: Synthesis of 5-bromo-2-(bromomethyl)pyridine (145)

A solution of 5-bromo-2-methylpyridine (50 g, 292.4 mmol), BPO (7.0 g, 29 mmol) and 2-bromocyclopentane-1,3-dione (56.8 g, 319 mmol) in CCl₄ (700 ml) was stirred under N₂ at 90° C. for 15 hr. The reaction mixture was filtered and concentrated to afford 145 (49.6 g). The compound was used in the next reaction without further purification. LCMS m/z 252 (M+1)⁺.

Step 2: Synthesis of 2-(5-bromopyridin-2-yl)acetonitrile (146)

A solution of 5-bromo-2-(bromomethyl)pyridine (49.6 g, 197.6 mmol), KCN (38.7 g, 595.4 mmol) and TBAB (4.5 g, 14.0 mmol) in DCM/H₂O (750 ml) was stirred at room temperature for 15 hr. The reaction mixture was diluted with H₂O (100 ml), extracted with DCM (3×70 ml). All of the organic layers were combined, washed with brine (2×50 ml), dried over Na₂SO₄, filtered and concentrated, then purified by silica gel column chromatography to afford 146 (26.2 g, 68% yield). LCMS m/z 197 (M+1)⁺.

Step 3: Synthesis of methyl 2-(5-bromopyridin-2-yl)acetate (147)

A solution of 2-(5-bromopyridin-2-yl) acetonitrile (26.2 g, 135.0 mmol) and SOCl₂ (100 ml) in anhydrous MeOH (150 ml) was stirred at room temperature for 15 hr. The reaction mixture was concentrated to dryness. Then the crude mixture was diluted with H₂O (100 ml) and the pH was adjusted to 7.0-8.0 with saturated aqueous NaHCO₃. The combined organic layers were washed with brine (2×50 ml), dried over Na₂SO₄, filtered and concentrated to dryness. The crude material was purified by silica gel column chromatography to afford the desired product (17.6 g, 57% yield). LCMS m/z 230 (M+1)⁺; ¹H NMR (400 MHz, CDCl₃) ppm: 8.61 (d, J=2.4 Hz, 1H), 7.81 (dd, J=8 Hz, 2.4 Hz, 1H), 7.23 (d, J=8.4 Hz, 1H), 3.82 (s, 2H), 3.73 (s, 3H).

Step 4: Synthesis of methyl 2-(5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-yl)acetate (148)

A mixture of compound methyl 2-(5-bromopyridin-2-yl)acetate (1 g, 4.35 mmol), 4,4,4′,4′,5,5,5′,5′-octamethyl-2,2′-bi(1,3,2-dioxaborolane) (1.33 g, 5.22 mmol), KOAc (850 mg, 8.7 mmol) and Pd(dppf)Cl₂ (100 mg) was heated at reflux in 15 mL of dry dioxane under N₂ for 18 h. This mixture was filtered and the filtrate was diluted with water (20 mL), extracted with EtOAc (3×20 mL), the organic layer was dried over Na₂SO₄, filtered and concentrated to afford 148 (1.2 g). The compound was used in the next reaction without further purification. LCMS m/z 278 (M+1)⁺.

Step 5: Synthesis of methyl 2-(2′-methyl-3,3′-bipyridin-6-yl)acetate (149)

A solution of methyl 2-(5-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)pyridin-2-yl)acetate (1.2 g, 4.6 mmol), KOAc (354 mg, 3.6 mmol) and Pd(dppf)Cl₂(100 mg, 0.14 mmol) in toluene/THF/H₂O (2:2:1, 20 ml) was stirred at 100° C. for 15 hr under N₂. The reaction mixture was diluted with H₂O (30 ml) and extracted with DCM (2×30 ml). The combined organic layers were washed with brine (2×20 ml), dried over Na₂SO₄, filtered and concentrated. The compound was purified by silica gel column chromatography to afford 149 (500 mg, 58%). LCMS m/z 243 (M+1)⁺.

Intermediate 151: Synthesis of methyl 2-(5-(2-methylthiazol-5-yl)pyridin-2-yl)acetate

Synthesis of methyl 2-(5-(2-methylthiazol-5-yl)pyridin-2-yl)acetate (151)

A solution of methyl 2-(5-bromopyridin-2-yl)acetate 147 (460 mg, 2.0 mmol), CsF (25 mg, 0.16 mmol) and Pd(dppf)Cl₂(20 mg, 0.027 mmol) in dry dioxane (6.0 ml) was stirred under N₂ at 120° C. for 15 hr. The reaction mixture was diluted with H₂O (20 ml), extracted with DCM (2×20 ml). The combined organic layers were washed with brine (2×20 ml), dried over Na₂SO₄, filtered and concentrated. The crude compound was purified by silica gel column chromatography to afford the desired product 151 (250 mg, 50%). LCMS: m/z 249 (M+1)⁺.

Intermediate 153: Synthesis of [6-(2-methyl-thiazol-5-yl)-pyridin-3-yl]acetic acid methyl ester

Step 1: Synthesis of 2-methyl-5-tributylstannanyl-thiazole (150)

N-butyllithium (5.2 mL, 13 mmol) was added dropwise to a solution of 2-methyl-thiazole (1 g, 10 mmol) in anhydrous THF (20 mL) over 10 min. The mixture was stirred at −78° C. for 1 hr under N₂. The mixture was treated with a solution of tributyltin chloride (3.91 g, 12 mmol) in anhydrous THF (10 mL) over 10 min. The mixture was stirred at −78° C. for another 1 hr under N₂. The mixture was warmed to r.t. for 16 hr. The mixture was quenched with water (20 mL), extracted with EtOAc (3×20 mL). The organic layer was washed with saturated NaHCO₃ (30 mL), brine (30 mL), dried over Na₂SO₄, filtered and concentrated to afford 150 which was used for the next step without further purification. (3.6 g). NMR (400 MHz, DMSO-d₆) ppm: 0.87-0.91 (m, 11H), 1.09-1.13 (m, 5H), 131-1.37 (m, 8H), 1.51-1.55 (m, 3H), 1.56-1.59 (m, 2.78 (s, 3H), 7.56 (s, 1H).

Step 2: Synthesis of [6-(2-methyl-thiazol-5-yl)-pyridin-3-yl]acetic acid methyl ester (153)

A a mixture of 2-Methyl-5-tributylstannanyl-thiazole (2.54 g, 6.52 mmol), (6-Bromo-pyridin-3-yl)-acetic acid methyl ester 142 (1 g, 4.35 mmol) in anhydrous dioxane (20 mL) was added Pd(PPh₃)₄ (100 mg) under N₂. The mixture was stirred at 80° C. for 16 h under N₂, then cooled to r.t. The mixture was concentrated and purified by column chromatography with silica gel (PE/ethyl acetate=10:1 to 5:1) to afford 153 (229 mg, 21%). LCMS: m/z 249 (M+1)⁺.

Intermediate 156: Synthesis of [6-(5-methyl-[1,2,4]oxadiazol-3-yl)-pyridin-3-yl]-acetic acid methyl ester

Step 1: Synthesis of (6-cyano-pyridin-3-yl)-acetic acid methyl ester (154)

To a mixture of (6-bromo-pyridin-3-yl)-acetic acid methyl ester (2 g, 8.7 mmol), Zn(CN)₂ (1.01 g, 8.7 mmol) and Zn (57 mg, 0.87 mmol) in anhydrous DMF (20 mL) was added Pd(dppf)Cl₂ (200 mg) and Pd₂(dba)₃ (200 mg) under N₂. The mixture was stirred at 120° C. for 1 h, then cooled to rt, diluted with water (50 mL), extracted with EtOAc (3×50 mL). The organic layer was combined and washed with water (3×50 mL), brine (2×50 mL), dried over Na₂SO₄, filtered and concentrated. The residue was purified by column chromatography with silica gel (PE/Ethyl Acetate=10:1-3:1) to afford 154 (1.08 g, 70%). LCMS: m/z 177 (M+1)⁺.

Step 2: Synthesis of [6-(N-hydroxycarbamimidoyl)-pyridin-3-yl]-acetic acid methyl ester (155)

To a solution of (6-cyano-pyridin-3-yl)-acetic acid methyl ester (1.08 g, 6.165 mmol) and NH₂OH hydrochloride salt (857 mg, 12.33 mmol) in MeOH (10 mL) was added NaHCO₃ (1.036 g, 12.33 mmol). The mixture was stirred at 70° C. for 1 hr. The reaction mixture was concentrated to dryness. The crude product was diluted with water (20 mL), extracted with EtOAc (2×20 mL). The organic layer was dried over Na₂SO₄, filtered and concentrated to afford 155 (1.29 g, 84%). LCMS: m/z 210 (M+1)⁺.

Step 3: Synthesis of [6-(5-methyl-[1,2,4]oxadiazol-3-yl)-pyridin-3-yl]-acetic acid methyl ester (156)

To a solution of [6-(N-hydroxycarbamimidoyl)-pyridin-3-yl]acetic acid methyl ester (500 mg, 2.39 mmol) in Ac₂O (5 mL) was heated to reflux for 16 hr. The mixture was concentrated to remove Ac₂O, diluted with EtOAc (10 mL), washed with NaHCO₃ (3×10 mL), brine (2×10 mL), dried over Na₂SO₄, filtered and concentrated to afford 156 (557 mg). The compound was used in the next reaction without further purification. LCMS: m/z 234 (M+1)⁺.

The compounds outlined in the table below are synthesized using the general method in Example 134 using the appropriate aniline D, appropriate aldehyde A and appropriate phenylacetic acetate intermediate B.

LCMS LCMS Ex. Structure MW Method Ion Rt 236

531.7 G 532 1.85 237

531.7 J 266 1.80 238

537.7 J 269 2.48 239

537.7 G 538 2.65 240

522.6 G 523 2.53 241

522.6 G 523 2.53

Examples 244-254

The compounds outlined below are synthesized using the general method in Example 134 using the appropriate aniline D, appropriate aldehydes A and appropriate phenylacetic acetate intermediate B. Some examples of phenyl acetate intermediates are outlined below.

Intermediate 157: Synthesis of methyl 2-(2-chloro-4-(2-methylthiazol-5-yl)phenyl)acetate

Synthesis of methyl 2-(2-chloro-4-(2-methylthiazol-5-yl)phenyl)acetate (157)

To a solution of methyl 2-(4-bromo-2-chlorophenyl)acetate (5 g, 18.98 mmol) in DMA (50 mL) was added 2-methylthiazole (2.82 g, 28.44 mmol), AcOK (2.79 g, 28.43 mmol) and Pd(PPh₃)₄ (1.10 g, 0.95 mmol) under nitrogen. The reaction mixture stirred at 100° C. for 15 h and then filtered. The filtrate was washed with water and extracted with EtOAc (2×50 mL). The combined layers were washed with brine (5×30 mL), dried over Na₂SO₄ and concentrated. The residue was purified by column chromatography on silica gel to afford 157 (4.3 g, 80%).

LCMS m/z 282 (M+1)⁺.

Intermediate 158: Synthesis of methyl 2-(2-chloro-4-(2-methylthiazol-5-yl)phenyl)acetate

Synthesis of methyl 2-(2-chloro-4-(2-methyloxazol-5-yl)phenyl)acetate (158)

A solution of methyl 2-(4-bromo-2-chlorophenyl)acetate (2.12 g, 8.02 mmol, 1.0 eq), 2-methyloxazole (1.0 g, 1.5 eq), AcOK (1.18 g, 2.0 eq), Pd(PPh₃)₄(463 mg, 0.05 eq) in DMA (20 mL) was stirred at 90° C. for 16 h under N₂. After cooling, water (200 mL) was added to the reaction mixture and extracted with DCM (3×10 mL). The combined organic layers were washed with water, dried over Na₂SO₄, filtered and concentrated. The crude mixture was purified by silica gel column chromatography (PE:ethyl acetate=10:1) to afford 158 (900 mg, 42%) as a white solid. ¹H NMR (400 MHz, CDCl₃) ppm: 7.64 (d, J=2 Hz, 1H), 7.47 (dd, J=8 Hz, 2 Hz, 1H), 7.33 (d, J=8 Hz, 1H), 7.21 (s, 1H), 3.79 (s, 2H), 3.72 (s, 3H), 2.54 (s, 3H).

LCMS LCMS Ex. Structure MW Method Ion Rt 244

545.1 E 545 2.79 245

557.1 D 557 3.11 246

531.1 E 531 2.63 247

597.2 D 299 3.36 248

571.1 E 572 3.51 249

587.1 G 294 3.07 250

573.2 D 573 3.30 251

555.1 G 555 3.01 252

575.1 G 575 3.07 253

536.7 G 538 3.02 254

550.7 G 552 3.25

Examples 255-275

The compounds in examples 255-275 were synthesized using the general procedure outlined in Example 134. In these cases in the final step, the appropriate Boc protected aniline (1 eq) was reacted with the chloropyrimidine intermediate (1 eq) in DMSO and stirred at 100-120° C. for 16 h. This crude mixture was directly purified by preparative HPLC to afford pure Boc protected compounds. After isolation, the Boc protected piperidines were deprotected using acidic conditions to afford the final compounds after evaporation to dryness. In some cases the compounds were washed with basic solutions to afford the free base.

The compounds outlined in the table below are synthesized using the general method in Example 134 using the appropriate boc-protected aniline D, appropriate aldehyde A and appropriate phenylacetic acetate intermediate B.

Example of a Synthesis of Boc Protected Aniline Intermediate 159: Synthesis of 4-(4-amino-phenyl)-piperidine-1-carboxylic acid tert-butyl ester

Synthesis of 4-(4-amino-phenyl)-piperidine-1-carboxylic acid tert-butyl ester (159)

To a solution of 4-(4-amino-phenyl)-3,6-dihydro-2H-pyridine-1-carboxylic acid tert-butyl ester (33 g, 0.12 mol) in MeOH (1 L) was added Pd/C (5 g) under Ar. The mixture was stirred at r.t. under an atmosphere of H₂ (40 psi) for 3 hr. The mixture was filtered and concentrated to afford the desired compound 159 (32.3 g, 97%). LCMS m/z 221 (M-55)⁺.

The remaining boc-protected anilines were prepared in a similar fashion.

LCMS LCMS Ex. Structure MW Method Ion Rt 255

560 E 560 2.89 256

557.1 E 557 2.87 257

594.1 E 595 2.65 258

551.1 E 551 1.96 259

551.1 E 551 1.95 260

569.1 E 285 2.06 261

569.1 E 569 2.07 262

537.0 F 537 0.95 263

555.1 E 555 2.87 264

549.1 E 549 0.84 265

553 F 553 1.05 266

555.1 F 555 1.14 265

540.0 D 271 3.16 266

612.1 D 307 3.21 267

569.1 E 569 3.07 268

569.1 E 569 3.02 269

587.1 E 587 3.15 270

573.1 E 573 3.00 271

580.1 D 582 2.97 272

587.1 E 587 3.72 273

541.0 E 541 3.32 274

543.1 D 543 3.07 275

542.0 E 542 1.07

Examples 276-283

The compounds in examples 276-283 were synthesized synthesized using the procedure outlined in example 40 using the appropriate aniline in step 3 and using the appropriate boronic acid in step 4. In the examples with a secondary amine on the piperidine, the appropriate Boc-protected aminoaniline was used and in the final step, the Boc protecting group was removed under acidic conditions.

LCMS LCMS Ex. Structure MW Method Ion Rt 276

579.1 F 579 2.75 277

551.1 D 551 2.29 278

569.07 E 571 2.08 279

566.1 H 566 2.68 280

580.1 H 291 2.64 281

624.1 H 313 2.86 282

570.1 H 570 2.87 283

620.1 H 311 3.34

Example 284 Synthesis of 8-ethyl-6-(2-fluoro-4-(2-methylpyridin-3-yl)phenyl)-2-(4-(1-methylpiperidin-4-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one Intermediate 164: Synthesis of methyl 2-(2-fluoro-4-(2-methylpyridin-3-yl)phenyl)acetate

Step 1: Synthesis of 4-bromo-1-(bromomethyl)-2-fluorobenzene (161)

To a solution of 4-bromo-2-fluoro-1-methylbenzene (6 g, 31.75 mmol) in CCl₄ (50 mL) was added NBS (6.22 g, 34.94 mmol) and BPO (384 mg, 1.59 mmol) under nitrogen, the reaction mixture was stirred at 80° C. for 15 h. The mixture was washed with water, extracted with DCM (2×50 mL). The combined layers were washed with brine (100 mL), dried over Na₂SO₄ and concentrated to afford 161 (9 g) which was used for the next step without further purification. LCMS m/z 269 (M+1)⁺.

Step 2: Synthesis of 2-(4-bromo-2-fluorophenyl)acetonitrile (162)

To a solution of 4-bromo-1-(bromomethyl)-2-fluorobenzene (9 g, crude) in DCM (50 mL) and H₂O (50 mL) was added KCN (6.56 g, 100.74 mmol) and TBAB (1 g) and stirred at for 15 h. The mixture was washed with water, extracted with DCM (2×50 mL). The combined layers were washed with brine (100 mL), dried over Na₂SO₄ and concentrated to afford 162 (7 g) which was used in the next step without further purification. LCMS m/z 214 (M+1)⁺.

Step 3: Synthesis of methyl 2-(4-bromo-2-fluorophenyl)acetate (163)

To a solution of 2-(4-bromo-2-fluorophenyl) acetonitrile (7 g, crude) in MeOH (50 mL) was added dropwise SOCl₂ (35 mL) at 0° C. The mixture was stirred at r.t. for 15 h: The solvent was removed. The residue was washed with water and extracted with EtOAc (3×50 mL). The combined layers were washed with brine (50 mL), dried over Na₂SO₄ and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-10% EtOAc in petroleum ether to afford 163 (5 g, 68%). LCMS m/z 247 (M+1)⁺.

Step 4: Synthesis of methyl 2-(2-fluoro-4-(2-methylpyridin-3-yl)phenyl)acetate (164)

To a solution of methyl 2-(4-bromo-2-fluorophenyl)acetate (1 g, 4.05 mmol) in toluene/THF/H₂O (15 mL, v/v/v=2/2/1) were added 2-methylpyridin-3-ylboronic acid (870 mg, 3.97 mmol), AcOK (790 mg, 8.05 mmol) and PdCl₂(dppf) (222 mg, 0.31 mmol) under nitrogen. The reaction mixture was stirred at 90° C. for 15 h. The reaction mixture was filtered, the filtrate was washed with water, extracted with EtOAc (2×10 mL). The combined layers were washed with brine, dried over Na₂SO₄ and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-10% EtOAc in petroleum ether to afford the desired product (0.9 g, 86%). LCMS m/z 260 (M+1)⁺.

Example 284 was synthesized using the procedure from Example 134 with the phenylacetate intermediate 164.

LCMS LCMS Ex. Structure MW Method Ion Rt 284

548.7 E 549 1.79

Example 285 Synthesis of 6-(2-chloro-5-methyl-4-(pyridin-3-yl)phenyl)-8-ethyl-2-(4-(1-methylpiperidin-4-yl)phenylamino)pyrido[2,3-d]pyrimidin-7(8H)-one Intermediate 172: Synthesis of methyl 2-(2-chloro-5-methyl-4-(pyridin-3-yl) phenyl)acetate

Step 1: Synthesis of 1-bromo-2-chloro-5-methyl-4-nitrobenzene (166)

A solution of 2-bromo-1-chloro-4-methylbenzene (24 g, 0.117 mol, 1 eq) in conc. H₂SO₄ (200 mL) was cooled to 0-5° C. Nitric acid (5 mL, 1.48 g/ml, 0.117 mol) in conc. H₂SO₄ (13 ml) was added dropwise to the mixture slowly. After the addition was completed, the reaction was stirred at 0° C. for 3 h. The reaction mixture was poured into 200 g ice-water and extracted with DCM (2×100 mL). The combined organic layers were washed with water, dried over Na₂SO₄, and concentrated to afford crude 166, which was recrystallized from ethanol (200 ml) to afford 166 as a pale yellow solid (24 g, 83%). ¹H NMR (400 MHz, CDCl₃) ppm: 8.08 (s, 1H), 7.63 (s, 1H), 2.56 (s, 3H).

Step 2: Synthesis of 1-allyl-2-chloro-5-methyl-4-nitrobenzene (167)

A solution of 1-bromo-2-chloro-5-methyl-4-nitrobenzene (6.4 g 25.6 mmol), allylSnBu₃(11.17 g, 33.3 mmol), Pd(PPh₃)₄ (2.9 g, 2.56 mmol) in dry dioxane (100 ml) was stirred at 90° C. for 16 h. The mixture was concentrated and purified by silical gel column chromatography. The target was dissolved in DCM, washed with saturated aq CsF, dried over Na₂SO₄, and concentrated to afford 167 (5 g, 93%). ¹H NMR (400 MHz, CDCl₃) ppm: 8.02 (s, 1H), 7.19 (s, 1H), 5.96-5.89 (m, 1H), 5.19-5.08 (m, 2H), 3.52 (d, J=6.4 Hz, 2H), 2.56 (s, 3H).

Step 3: Synthesis of 2-(2-chloro-5-methyl-4-nitrophenyl) acetic acid (168)

A solution of NaIO₄ (39 g, 5.0 eq) in H₂O (200-400 mL) was added dropwise slowly to a solution of compound 1-allyl-2-chloro-5-methyl-4-nitrobenzene (7.8 g, 36.86 mmol, 1.0 eq), RuCl₃.H₂O (390 mg, 2.24 mol %), Bu₄NI(1.37 g, 3.69 mmol) in ethyl acetate (200 mL) at 0° C. The reaction mixture was stirred at room temperature for 1-2 h. The aqueous layer was extracted with ethyl acetate. The combined organic layers were washed with 1N HCl, dried over Na₂SO₄, filtered and concentrated to afford 168 (7.8 g) which was used the next step directly without further purification. ¹H NMR (400 MHz, CDCl₃) ppm: 8.03 (s, 1H), 7.28 (s, 1H), 3.84 (s, 2H), 2.56 (s, 3H).

Step 4: Synthesis of methyl 2-(2-chloro-5-methyl-4-nitrophenyl)acetate (169)

A solution of 2-(2-chloro-5-methyl-4-nitrophenyl) acetic acid (7.8 g, 34 mmol, 1.0 eq) in SOCl₂(150 ml) was heated to 100° C. for 4 h. The mixture was concentrated and purified by silical gel column chromatography (PE:ethyl acetate=30:1) to afford 169 (5.3 g, 64%). ¹H NMR (400 MHz, CDCl₃) ppm: 8.04 (s, 1H), 7.27 (s, 1H), 3.79 (s, 2H), 3.72 (s, 3H), 2.56 (s, 3H).

Step 5: Synthesis of methyl 2-(4-amino-2-chloro-5-methylphenyl)acetate (170)

NaBH₄ (2.35 g, 3.0 eq) was added in portions to a solution of methyl 2-(2-chloro-5-methyl-4-nitrophenyl)acetate (5.3 g, 21.8 mmol, 1.0 eq) and NiCl₂.6H₂O (10.4 g, 2.0 eq) in MeOH(150 mL) at 0° C. over 10 min. The reaction was stirred at r.t. for 30 min. The mixture was quenched with saturated aqueous NH₄Cl and H₂O (300 mL) was added. The mixture was extracted with DCM (4×20 mL), dried over Na₂SO₄, filtered and concentrated. The crude material was purified by silical gel column chromatography (PE: ethyl acetate=10:1) to afford 170 (3 g, 65%). LCMS: m/z 214 (M+1)⁺.

Step 6: Synthesis of methyl 2-(4-bromo-2-chloro-5-methylphenyl)acetate (171)

To a solution of methyl 2-(4-amino-2-chloro-5-methylphenyl)acetate (2.0 g, 4.68 mmol, 1.0 eq), t-BuONO (580 mg, 1.2 eq), p-TsOH (972 mg, 1.2 eq), TBAB (3.0 g, 2.0 eq) in CH₃CN (50 mL), was added CuBr (14.4 mg, 1 mol %). The reaction was stirred at room temperature for 3-4 h, then concentrated. The mixture was dissolved in DCM (30 mL), washed with saturated aq NaHCO₃ (8×20 mL), H₂O (2×10 mL), dried over Na₂SO₄, filtered and concentrated to afford 171 (2.2 g), which was used the next step directly without further purification. ¹H NMR (400 MHz, CDCl₃) ppm: 7.54 (s, 1H), 7.13 (s, 1H), 3.72 (s, 3H), 3.67 (s, 2H), 2.38 (s, 3H).

Step 7: Synthesis of methyl 2-(2-chloro-5-methyl-4-(pyridin-3-yl) phenyl)acetate (172)

A mixture of methyl 2-(4-bromo-2-chloro-5-methylphenyl)acetate (280 mg, 1 mmol, 1.0 eq), pyridin-3-ylboronic acid (140 mg, 1.2 eq), K₂CO₃ (276 mg, 2.0 eq), and Pd(dppf)Cl₂ in toluene/THF/H₂O (5 mL, 2:2:1) was stirred at 90° C. for 4 h under N2. Water (30 mL) was added to the reaction mixture. The mixture was extracted with ethyl acetate (3×10 ml), dried over Na₂SO₄, filtered and concentrated. The crude material was purified by silical gel column chromatography (PE: ethyl acetate=10:1) to afford 172 (210 mg, 76%) as a white solid. LCMS: m/z 276 (M+1)⁺.

Example 285 was synthesized using the procedure from Example 134 with the phenylacetate intermediate 172.

LCMS LCMS Ex. Structure MW Method Ion Rt 285

565.1 G 567 2.21

Examples 286-288 Intermediate 182: Synthesis of methyl 2-(2-chloro-5-(dimethylamino)-4-(pyridin-3-yl) phenyl) acetate

Step 1: Synthesis of methyl 4-bromo-2-chlorobenzoate (174)

A solution of compound 4-bromo-2-chlorobenzoic acid (50 g, 212 mmol, 1.0 eq) in SOCl₂ (500 mL) was heated to 100° C. for 4 h and then concentrated to dryness. The residue was dissolved in cold methanol (500 mL), and stirred for 15 min. The crude material was purified by silical gel column chromatography to afford 174 (PE: ethyl acetate=30:1) (5.3 g, 64%). NMR (400 MHz, CDCl₃) ppm: 7.73 (d, J=8.4 Hz, 1H), 7.64 (d, J=1.6 Hz, 1H), 7.46 (dd, J=8.4 Hz, 1.6 Hz, 1H), 3.93 (s, 3H).

Step 2: Synthesis of methyl 4-bromo-2-chloro-5-nitrobenzoate (175)

Nitric acid (0.86 mL, 1.48 g/ml, 20.04 mmol) in con.H₂SO₄ (3 mL) was added dropwise slowly to the mixture of methyl 4-bromo-2-chlorobenzoate (5 g, 20.04 mmol, leg) in conc.H₂SO₄ (50 mL) at 0-5° C. The mixture was stirred at 0° C. for 3 h and then poured into 100 g ice-water, extracted with DCM (2×20 mL). The combined organic layers were washed with water, dried over Na₂SO₄, and concentrated to afford 175 (3.2 g), which was used the next step directly without further purification. ¹H NMR (400 MHz, CDCl₃) ppm: 8.42 (s, 1H), 7.98 (s, 1H), 3.97 (s, 3H).

Step 3: Synthesis of methyl 5-amino-4-bromo-2-chlorobenzoate (176)

To a solution of methyl 4-bromo-2-chloro-5-nitrobenzoate (2.2 g, 7.47 mmol, 1.0 eq) and NiCl₂.6H₂O (3.55 g, 2.0 eq,) in MeOH (50 mL), which was cooled to 0° C., then NaBH₄ (807 mg, 3.0 eq) was added in portions and stirred at r.t. for 30 min. The reaction was quenched with saturated aqueous NH₄Cl followed by 100 mL H₂O. The mixture was extracted with DCM (3×20 mL). The combined organic layers were dried over Na₂SO₄, filtered and concentrated. The crude material was purified by silica gel column chromatography (PE: ethyl acetate=10:1) to afford 176 (1.8 g, 91%). ¹H NMR (400 MHz, CDCl₃) ppm: 8.03 (s, 1H), 7.28 (s, 1H), 3.84 (s, 2H), 2.56 (s, 3H). NMR (400 MHz, CDCl₃) ppm: 7.48 (s, 1H), 7.21 (s, 1H), 4.2 (brs, 2H), 3.88 (s, 3H). LCMS m/z 266 (M+1)⁺.

Step 4: Synthesis of methyl 4-bromo-2-chloro-5-(dimethylamino)benzoate (177)

A solution of methyl 5-amino-4-bromo-2-chlorobenzoate (900 mg, 3.4 mmol) and HCHO (10 mL) in HCOOH (10 mL) was stirred at 100° C. for 2 h. The mixture was concentrated to dryness. DCM (20 mL) was added, and the pH adjusted to pH=8 with saturated aq. Na₂CO₃. The aqueous solution was extracted with DCM (2×20 mL). The combined organic layers were dried over Na₂SO₄, filtered and concentrated. The crude material was purified by silica gel column chromatography (PE: ethyl acetate=20:1) to afford 177 (600 mg, 60%) as a pale yellow oil. ¹H NMR (400 MHz, CDCl₃) ppm: 7.63 (s, 1H), 7.48 (s, 1H), 3.91 (s, 3H), 2.79 (s, 6H).

LCMS m/z 292 (M+1)⁺.

Step 5: Synthesis of (4-bromo-2-chloro-5-(dimethylamino) phenyl) methanol (178)

LiAlH₄ (182 mg, 1.0 eq) was added in portions to a solution of methyl 4-bromo-2-chloro-5-(dimethylamino)benzoate (1.4 g, 4.9 mmol, 1.0 eq) in dry THF (20 mL) at 0° C. The reaction was stirred at 0-10° C. for 2 h. The mixture was quenched with 1.4 mL of water, 1.4 mL of 15% aqueous NaOH and 4.2 mL of water, dried over MgSO₄ and filtered. The solution was concentrated to afford 178 (1.4 g) as an off-white solid, which was used the next step without further purification. LCMS m/z 264 (M+1)⁺.

Step 6: Synthesis of 2-bromo-5-(bromomethyl)-4-chloro-N,N-dimethylaniline (179)

PBr₃(1.23 g, 1.0 eq, 431 uL, d=2.852 g/ml) was added dropwise to a solution of (4-bromo-2-chloro-5-(dimethylamino) phenyl) methanol (1.2 g, 4.54 mmol, 1.0 eq) in dry DCM (20 mL) at 0° C. The mixture reaction was stirred at r.t. for 3 h. The mixture was washed with water (2×10 mL), dried over Na₂SO₄ and filtered. The filtrate was concentrated to afford 179 (1.4 g) as an off-white solid, which was used the next step directly without further purification. ¹H NMR (400 MHz, CDCl₃) ppm: 7.51 (s, 1H), 7.02 (s, 1H), 4.44 (s, 2H), 2.73 (s, 6H).

Step 7: Synthesis of 2-(4-bromo-2-chloro-5-(dimethylamino)phenyl)acetonitrile (180)

A solution of 2-bromo-5-(bromomethyl)-4-chloro-N,N-dimethylaniline (1.2 g, 3.6 mmol, 1.0 eq), KCN (700 mg, 3.0 eq), TBAB (200 mg, 0.1 eq) in DCM/H₂O (30 mL, 1:2) was stirred at r.t. for 16 h. H₂O (10 mL) was added to this mixture, and extracted with DCM (2×10 mL). The combined organic layers were washed with saturated aq. NaHCO₃ and H₂O, dried over Na₂SO₄, and filtered. The filtrate was concentrated and the residue was purified by silica gel column chromatography (PE: ethyl acetate=15:1) to afford 180 (900 mg, 90%). ¹H NMR (400 MHz, CDCl₃) ppm: 7.58 (s, 1H), 7.15 (s, 1H), 3.75 (s, 2H), 2.80 (s, 6H).

Step 8: Synthesis of methyl 2-(4-bromo-2-chloro-5-(dimethylamino) phenyl)acetate (181)

SOCl₂(10 mL) was added dropwise to a solution of compound 2-(4-bromo-2-chloro-5-(dimethylamino) phenyl) acetonitrile (1 g, 3.66 mmol, 1.0 eq) in MeOH (20 mL). The reaction mixture was stirred at r.t. for 16 h. The mixture was concentrated, dissolved in DCM (20 mL), washed with H₂O, dried over Na₂SO₄ and filtered. The filtrate was concentrated and purified by silica gel column chromatography (PE: ethyl acetate=15:1) to afford 181 (500 mg, 45%). ¹H NMR (400 MHz, CDCl₃) ppm: 7.56 (s, 1H), 6.96 (s, 1H), 3.72 (s, 3H), 3.70 (s, 2H), 2.79 (s, 6H).

Step 9: Synthesis of methyl 2-(2-chloro-5-(dimethylamino)-4-(pyridin-3-yl) phenyl)acetate (182)

A solution of methyl 2-(4-bromo-2-chloro-5-(dimethylamino) phenyl)acetate (450 mg, 1.46 mmol, 1.0 eq), pyridin-3-ylboronic acid (215.5 mg, 1.2 eq), K₂CO₃ (405 mg, 2.0 eq), Pd(dppf)Cl₂ (200 mg, 0.2 eq) in toluene/THF/H₂O (10 mL, 2:2:1) was stirred at 90° C. for 3-4 h under N₂. Water (30 mL) was added to the reaction mixture. The mixture was extracted with ethyl acetate (3×10 mL), dried over Na₂SO₄ and filtered. The filtrate was concentrated and purified by silical gel column chromatography (PE: ethyl acetate=10:1) to afford 182 (400 mg, 89%) as a white solid. ¹H NMR (400 MHz, CDCl₃) ppm: 8.77 (d, J=2 Hz, 1H), 8.54 (dd, J=4.8 Hz, 1.6 Hz, 1H), 7.89 (dd, J=8 Hz, 2 Hz, 1H), 7.32 (dd, J=8 Hz, 4.8 Hz, 1H), 7.20 (s, 1H), 6:94 (s, 1H), 3.76 (s, 2H), 3.74 (s, 3H), 2.79 (s, 6H).

Example 286 was synthesized using the procedure from Example 134 with the phenylacetate intermediate 182. Examples 287-288 were prepared using the procedure from Example 134 with the appropriate phenylacetate intermediate 182.

LCMS LCMS Ex. Structure MW Method Ion Rt 286

554.1 J 278 2.12 287

568.1 J 284 3.79 288

582.1 J 291 1.84

Examples 289-292 Intermediate 186: Synthesis of methyl 2-(4-bromo-2-chloro-5-fluorophenyl)acetate

Step 1: Synthesis of 1-bromo-4-(bromomethyl)-5-chloro-2-fluorobenzene (184)

To a solution of 1-bromo-5-chloro-2-fluoro-4-methylbenzene (14 g, 63.06 mmol) in CCl₄ (120 mL) were added NBS (12.2 g, 69.37 mmol) and BPO (762 mg, 3.15 mmol) under nitrogen. The reaction mixture was stirred at 80° C. for 15 h. The mixture was cooled, washed with water, extracted with DCM (2×50 mL). The combined organic layers were washed with brine (1×100 mL), dried over Na₂SO₄ and concentrated to give 199 (16 g, crude) which was used for the next step without further purification. LCMS m/z 303.4 (M+1)⁺.

Step 2: Synthesis of 2-(4-bromo-2-chloro-5-fluorophenyl)acetonitrile (185)

To a solution of 1-bromo-4-(bromomethyl)-5-chloro-2-fluorobenzene (16 g, crude) in DCM (100 mL) and H₂O (100 mL) was added KCN (12.3 g, 189.18 mmol) and TBAB (2.0 g). The reaction was stirred at r.t. for 15 h. The mixture was cooled, washed with water, extracted with DCM (2×500 mL). The combined layers were washed with brine (1×100 mL), dried over Na₂SO₄ and concentrated to give 200 (7 g, crude) which was used in the next step without further purification.

Step 3: Synthesis of methyl 2-(4-bromo-2-chloro-5-fluorophenyl)acetate (186)

To a solution of 2-(4-bromo-2-chloro-5-fluorophenyl)acetonitrile (7 g, crude) in MeOH (50 mL) was added drop-wise SOCl₂ (35 mL) with ice-water bath. The mixture was stirred at r.t. for 15 h. The solvent was removed in vacuo. The residue was washed with water and extracted with EtOAc (3×50 mL). The combined layers were washed with brine (1×50 mL), dried over Na₂SO₄ and concentrated. The residue was purified by column chromatography on silica gel eluted with 0-10% EtOAc in petroleum ether to give 5 g of the desired product.

LCMS m/z 282.5 (M+1)⁺.

LCMS LCMS Ex. Structure MW Method Ion Rt 289

575.1 G 575 3.07 290

560 G 560 2.81 291

569.1 G 569 1.91 292

573.1 G 573 2.89

Biological Examples Example 293 In Vitro PAK Inhibition Assay Assay Conditions

Compounds are screened in 1% DMSO (final) in the well. For 10 point titrations, 3-fold serial dilutions are conducted.

All Peptide/Kinase Mixtures are diluted to a 2× working concentration in the appropriate Kinase Buffer

Kinase Specific Assay Conditions. PAK1

The 2×PAK1/Ser/Thr 19 mixture is prepared in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. The final 10 μL Kinase Reaction consists of 2.71-30.8 ng PAK1 and 2 μM Ser/Thr 19 in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour Kinase Reaction incubation, 5 μL of a 1:128 dilution of Development Reagent A is added.

PAK2 (PAK65)

The 2×PAK2 (PAK65)/Ser/Thr 20 mixture is prepared in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. The final 10 μL Kinase Reaction consists of 0.29-6 ng PAK2 (PAK65) and 2 μM Ser/Thr 20 in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour Kinase Reaction incubation, 5 μL of a 1:256 dilution of Development Reagent A is added.

PAK3

The 2×PAK3/Ser/Thr 20 mixture is prepared in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. The final 10 μl, Kinase Reaction consists of 2.25-22 ng PAK3 and 2 μM Ser/Thr 20 in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour Kinase Reaction incubation, 5 μL of a 1:256 dilution of Development Reagent A is added.

PAK4

The 2×PAK4/Ser/Thr 20 mixture is prepared in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. The final 10 μL Kinase Reaction consists of 0.1-0.75 ng PAK4 and 2 μM Ser/Thr 20 in 50 mM HEPES pH 7.5, 0.01% BRIJ-35, 10 mM MgCl2, 1 mM EGTA. After the 1 hour Kinase Reaction incubation, 5 μL of a 1:256 dilution of Development Reagent A is added.

Assay Controls

The following controls are made for each individual kinase and are located on the same plate as the kinase:

0% Phosphorylation Control (100% Inhibition Control)

The maximum Emission Ratio is established by the 0% Phosphorylation Control (100% Inhibition Control), which contains no ATP and therefore exhibits no kinase activity. This control yields 100% cleaved peptide in the Development Reaction.

100% Phosphorylation Control

The 100% Phosphorylation Control, which consists of a synthetically phosphorylated peptide of the same sequence as the peptide substrate, is designed to allow for the calculation of percent phosphorylation.

This control yields a very low percentage of cleaved peptide in the Development Reaction.

The 0% Phosphorylation and 100% Phosphorylation Controls allow one to calculate the percent Phosphorylation achieved in a specific reaction well. Control wells do not include any kinase inhibitors.

0% Inhibition Control

The minimum Emission Ratio in a screen is established by the 0% Inhibition Control, which contains active kinase. This control is designed to produce a 10-50%* phosphorylated peptide in the Kinase Reaction.

Known Inhibitor

A known inhibitor control standard curve, 10 point titration, is run for each individual kinase on the same plate as the kinase to ensure the kinase is inhibited within an expected IC50 range previously determined.

The following controls are prepared for each concentration of Test Compound assayed:

Development Reaction Interference

The Development Reaction Interference is established by comparing the Test Compound Control wells that do not contain ATP versus the 0% Phosphorylation Control (which does not contain the Test Compound). The expected value for a non-interfering compound should be 100%. Any value outside of 90% to 110% is flagged.

Test Compound Fluorescence Interference

The Test Compound Fluorescence Interference is determined by comparing the Test Compound Control wells that do not contain the Kinase/Peptide Mixture (zero peptide control) versus the 0% Inhibition Control. The expected value for a non-fluorescence compound should be 0%. Any value >20% is flagged.

Assay Protocol

Bar-coded Corning, low volume NBS, black 384-well plate (Corning Cat. #3676)

-   -   1. Add the following solutions to a well in a 384-well plate:         -   2.5 μL of 4× Test Compound OR (100 nL 100× Test Compound             plus 2.4 μL kinase buffer)         -   5 μL of 2× Peptide/Kinase (PAK) Mixture         -   2.5 μL of 4×ATP Solution     -   2. Shake the plate for 30-seconds     -   3. Incubate the PAK Kinase Reaction at room temperature for         60-minutes     -   4. Add 5 μL of Development Reagent Solution to each well     -   5. Shake the plate for 30-seconds     -   6. Incubate the Development Reaction for 60-minutes     -   7. Determine the fluorescence using a fluorescence plate reader     -   8. Analyze the fluorescence data

Data Analysis

The following equations are used for each set of data points:

Equation Correction for Background Fluorescence FI_(Sample) − FI_(TCFI Ctl) Emission Ratio (using values corrected for background fluorescence) $\frac{{Coumarin}\mspace{14mu} {Emission}\mspace{14mu} \left( {445\mspace{14mu} {nm}} \right)}{{Fluorescein}\mspace{14mu} {Emission}\mspace{14mu} \left( {520\mspace{14mu} {nm}} \right)}$ % Phosphorylation (% Phos) $\left\{ {1 - \frac{\left( {{Emission}\mspace{14mu} {Ratio} \times F_{100\%}} \right) - C_{100\%}}{\left( {C_{0\%} - C_{100\%}} \right) + \left\lbrack {{Emission}\mspace{14mu} {Ratio} \times \left( {F_{100\%} - F_{0\%}} \right)} \right\rbrack}} \right\}*100$ % Inhibition $\left\{ {1 - \frac{\% \mspace{14mu} {Phos}_{Sample}}{\% \mspace{14mu} {Phos}_{0\% \mspace{14mu} {Inhibition}\mspace{14mu} {Ctl}}}} \right\}*100$ Z (using Emission Ratio values) $1 - \frac{{3*{Stdev}_{0\% \mspace{14mu} {Phos}\mspace{14mu} {Ctl}}} + {3*{Stdev}_{0\% \mspace{14mu} {Inhibition}}}}{{Mean}_{0\% \mspace{14mu} {Phos}\mspace{14mu} {Ctl}} - {Mean}_{0\% \mspace{14mu} {Inhibition}}}$ Difference Between Data Points (single point only) | % Inhibition_(Point 1) − % Inhibition_(Point 2) | Development Reaction Interference (DRI) (no ATP control) $\frac{{Emission}\mspace{14mu} {Ratio}_{{DRI}\mspace{14mu} {Ctl}}}{{Emission}\mspace{14mu} {Ratio}_{0\% \mspace{14mu} {Phos}\mspace{14mu} {Ctl}}}$ Test Compound Fluorescence Interference (TCFI) (check both Coumarin and Fluorescein emissions) $\frac{{FI}_{{TCFI}\mspace{14mu} {Ctl}}}{{FI}_{0\% \mspace{14mu} {Inhibitor}\mspace{14mu} {Ctl}}}$ FI = Fluorescence Intensity C100% = Average Coumarin emission signal of the 100% Phos. Control C0% = Average Coumarin emission signal of the 0% Phos. Control F100% = Average Fluorescein emission signal of the 100% Phos. Control F0% = Average Fluorescein emission signal of the 0% Phos. Control DRI = Development Reaction Interference TCFI = Test Compound Fluorescence Interference

Graphing Software

SelectScreen® Kinase Profiling Service uses XLfit from IDBS. The dose response curve is curve fit to model number 205 (sigmoidal dose-response model). If the bottom of the curve does not fit between −20% & 20% inhibition, it is set to 0% inhibition. If the top of the curve does not fit between 70% and 130% inhibition, it is set to 100% inhibition.

Table of Kinase ATP Km Bins and Inhibitor Validation

The table below provides specifications and data around each kinase. The representative IC50 value with a known inhibitor for each kinase was determined at the ATP bin nearest to the ATP Km app.

Z′-LYTE ATP Km app ATP Bin IC50 Kinase Substrate (μM) (μM) Inhibitor (nM) PAK1 Ser/Thr 19 48.5 50 Staurosporine 3.00 PAK2 Ser/Thr 20 89 75 Staurosporine 30.0 (PAK65) PAK3 Ser/Thr 20 101 100 Staurosporine 15.3 PAK4 Ser/Thr 20 3 5 Staurosporine 9.71

TABLE PAK Inhibition IC50 PAK1 PAK2 PAK3 PAK4 Structure IC50 (nM) IC50 (nM) IC50 (nM) IC50 (nM)

B C C D

B C D D

A A B D

B B B D

C C C D

D C D D

C B C D

C C D D

D D D D

A A A C

A A A C

A A A D

A A A C

A A A D

A A A D

A A A D

A A B D

A A B D

B B C D

A B B D

B B B D

B C C D

B C B D

A A A B

B D D D

B B B D

C D D D

A A A C

D D D D

D C C D

A A A D

B B C D

D D D D

D D D D

D D D D

A A A D

A A B C

B C D D

A B B D

D D D D

D D D D

D D D D

D D D D

D D D D

D D D D

D D D D

A A A B

A A A C

D D D D

D D D D

B B B D

A A A C

A B B D

A A A C

A A A C

A A B D

A A A C

A A A C

A A A C

A A A D

A A A D

A A A C

A A A C

A A A D

A A A C

A A A C

A A A D

A A A C

A A A B

A A A D

A A A C

A A A D

A A A D

A A A B

A A A D

A A B D

A A B D

A A A C

A A A D

A A A D

A A A C

A A A C

A A A D

A A A C

A A B D

A B B D

A A A C

A A B C

A A A C

A A A D

D B D D

A A A D

A A A C

A A A C

A A A C

A A A B

A A B D

A B B D

A A A C

A A A C

A A A C

A A B C

C B D D

A A B C

A A A D

B C C D

A A A C

D A A C

A A A D

A A A C

A A A C

A A A C

A A A C

A A A C

A A B C

A A A C

B B B D

A A B D

A A B D

A A B C

A B B C

A A A C

A A B C

A A A C

B B B C

A B B D

A A A C

A A A C

A A A B

A A A C

A A A B

A A B D

A A A D

A A A C

A A A D

A A B C

A A A C

B B B D

A A A C

B B B C

A A A C

B B D D

A A B C

A B B D

B A B C

A B B D

B B B C

A A B C

A A A C

A A A C

A A B D

A A A B

A B B D

A A A C

A A A B

A B B D

B B B C

A A A C

A A B C

A A A C

A A B D

A A A C

A B B D

A A B C

A B B D

A B B D

A A A C

A A A D

B B B D

A A A C

A A B D

A A B D

A A A C

A A A C

B B B C

B B B D

B B B D

A A A C

A B B D

A A A D

A A A D

A A A D

A A B D

A A A D

A A B C

A B B D

A A A C

A B B D

B B C D

B B C D

B B B C

A A A D

A A A C

B B B D

A A A C

A A B D

B B B D

C C C D

B B B D

B B B D

A A A B

A A A C

A A B C

B B B D

B B B D

A B B D

A A A C

A A B D

A A A B

A A A C

A A A C

A A B D

B B B D

A A A B

B B C D

A A B D

A A A B

A A A C

A A A C

A B B D

A A A C

A A A C

A B B D

B B B D

B B B D

A A B D

C C C D

A B B D

A A A C

A A B D

D D D D

C C C D

D D D D

A A A D

A A A D

C D D D

C C C D

B C C D

B C C D

A A B D

C D D D

A B A D

A A A D

A A A B

B B B D

A A B D

B B B D

A A B D

A A B C

A A A D

A A A D

B B C D

A A B C

C C D D

B C B D

B C B D

C C B D

C D D D

D D C D

B C B D

B D B D

C D B C

B B B D

D D D C

D D D D

D D D D

A A A C A, IC50 < 50 nM; B, 50 nM ≦ IC50 ≦ 500 nM; C, 0.5 μM < IC50 < 5 μM; D, IC50 ≧ 5 μM

Example 294 Treatment of Schizophrenia by Administration of a PAK Inhibitor Compound Disclosed Herein in an Animal Model

The ability of a PAK inhibitor to ameliorate behavioral and anatomical symptoms of schizophrenia (i.e., their mouse analogs) is tested in a dominant-negative DISCI mouse model of schizophrenia (Hikida et al (2007), Proc Natl Acad Sci USA, 104(36):14501-14506).

Forty DISC1 mice (ages 5-8 months) on a C57BL6 strain background are divided into treatment group (1 mg/kg of compound disclosed herein, oral gavage) and a placebo group (0.1% DMSO in physiological saline solution) and analyzed for behavioral differences in open field, prepulse inhibition, and hidden food behavioral tests, with an interval of about one week between each type of test. In the open field test, each mouse is placed in a novel open field box (40 cm×40 cm; San Diego Instruments, San Diego, Calif.) for two hours. Horizontal and vertical locomotor activities in the periphery as well as the center area are automatically recorded by an infrared activity monitor (San Diego Instruments). Single breaks are reported as “counts.” In this behavioral test, a significant reduction in total activity in the treatment group relative to the placebo group indicates a possible treatment effect.

In the hidden food test, mice are food-deprived for 24 h. After habituation to a new cage for 5 min, a food pellet is hidden under the cage bedding. The time it takes for the mouse to find the food pellet is measured until a maximum of 10 min is reached. In this behavioral test, a significant reduction in time to find the food pellet in the treatment group relative to the placebo group is indicative of a successful treatment effect.

In the prepulse inhibition test, acoustic startle and prepulse inhibition responses are measured in a startle chamber (San Diego Instruments). Each mouse is individuated to six sets of seven trail types distributed pseudorandomly: pulse-alone trials, prepulse-pulse trials, and no-stimulus trials. The pulse used is 120 dB and the prepulse is 74 dB. A significant increase in the prepulse inhibition response in the treatment group relative to the placebo group is indicative of a successful treatment effect.

In the forced swim test, each mouse is put in a large plastic cylinder, which is half-filled with room temperature water. The test duration is 6 min, during which the swim/immobility times are recorded. In this behavioral test, a significant reduction in immobility in the treatment group relative to the placebo group is indicative of a successful treatment effect.

In order to evaluate the ability of the compounds disclosed herein to alter brain morphology, an MRI study is conducted on placebo-treated and treated groups of DISC1-DN mice. In vivo MRI experiments are performed on an 11.7T Bruker Biospec small animal imaging system. A three-dimensional, fast-spin echo, diffusion weighted (DW) imaging sequence with twin navigation echoes is used to assess the ratio of lateral ventricle volume to total brain volume. A decrease in this ratio in the treated group relative to the ratio observed in the placebo-group is indicative of a successful treatment effect.

Statistical Analysis. Statistical analysis is performed by ANOVA or repeated ANOVA. Differences between groups are considered significant at p<0.05.

Example 295 In Vivo Monitoring of Dendritic Spine Plasticity in Double Transgenic GFP-M/DN-DISC1 Mice Treated with a PAK Inhibitor Compound Disclosed Herein

In the following experiment, dendritic spine plasticity is directly monitored in vivo by two photon laser scanning microscopy (TPLSM) in double transgenic GFP-M/DN-DISC1 mice treated with a compound disclosed herein or a placebo. Mice (C57BL/6) expressing GFP in a subset of cortical layer 5 neurons (transgenic line GFP-M described in Feng et al, 2000, Neuron 28:41-51) are crossed with DN-DISC1 C57BL/6 DN-DISC1 mice (Hikida et al (2007), Proc Natl Acad Sci USA, 104(36):14501-14506) to obtain heterozygous transgenic mice, which are then crossed to obtain homozygous double transgenic GFPM/DN-DISC1 mice used in this study.

GFP-M/DN-DISC1 animals aged 28-61 d are anesthetized using avertin (16 μl/g body weight; Sigma, St. Louis, Mo.). The skull is exposed, scrubbed, and cleaned with ethanol. Primary visual, somatosensory, auditory, and motor cortices are identified based on stereotaxic coordinates, and their location is confirmed with tracer injections (see below).

Long-term imaging experiments are started at P40. The skull is thinned over the imaging area as described in Grutzendler et al, (2002), Nature, 420:812-816. A small metal bar is affixed to the skull. The metal bar is then screwed into a plate that connected directly to the microscope stage for stability during imaging. The metal bar also allows for maintaining head angle and position during different imaging sessions. At the end of the imaging session, animals are sutured and returned to their cage. Thirty animals previously imaged at P40 are then divided into a control group receiving a 1% sugar solution (oral gavage once per day) and a treatment group administered a compound disclosed herein, in 0.1% DMSO (oral gavage. 1 mg/kg, once per day). During the subsequent imaging sessions (at P45, P50, P55, or P70), animals are reanesthetized and the skull is rethinned. The same imaging area is identified based on the blood vessel pattern and gross dendritic pattern, which generally remains stable over this time period.

At the end of the last imaging session, injections of cholera toxin subunit B coupled to Alexa Fluor 594 are made adjacent to imaged areas to facilitate identification of imaged cells and cortical areas after fixation. Mice are transcardially perfused and fixed with paraformaldehyde, and coronal sections are cut to verify the location of imaged cells. Sections are then mounted in buffer, coverslipped, and sealed. Images are collected using a Fluoview confocal microscope (Olympus Optical, Melville, N.Y.).

For in vivo two photon imaging, a two-photon laser scanning microscope is used as described in Majewska et al, (2000), Pflügers Arch, 441:398-408. The microscope consists of a modified Fluoview confocal scan head (Olympus Optical) and a titanium/sulphur laser providing 100 fs pulses at 80 MHz at a wavelength of 920 nm (Tsunami; Spectra-Physics, Menlo Park, Calif.) pumped by a 10 W solid-state source (Millenia; Spectra-Physics). Fluorescence is detected using photomultiplier tubes (HC125-02; Hamamatsu, Shizouka, Japan) in whole-field detection mode. The craniotomy over the visual cortex is initially identified under whole-field fluorescence illumination, and areas with superficial dendrites are identified using a 20×, 0.95 numerical aperture lens (IR2; Olympus Optical). Spiny dendrites are further identified under digital zoom (7-10×) using two-photon imaging, and spines 50-200 μm below the pial surface are studied. Image acquisition is accomplished using Fluoview software. For motility measurements, Z stacks taken 0.5-1 μm apart are acquired every 5 min for 2 h. For synapse turnover experiments, Z stacks of dendrites and axons are acquired at P40 and then again at P50 or P70. Dendrites and axons located in layers 1-3 are studied. Although both layer 5 and layer 6 neurons are labeled in the mice used in this study, only layer 5 neurons send a clear apical dendrite close to the pial surface thus, the data will come from spines on the apical tuft of layer 5 neurons and axons in superficial cortical layers.

Images are exported to Matlab (Math Works, Natick, Mass.) in which they are processed using custom-written algorithms for image enhancement and alignment of the time series. For motility measurements (see Majewska et al, (2003), Proc Natl Acad Sci USA, 100:16024-16029) spines are analyzed on two-dimensional projections containing between 5 and 30 individual images; therefore, movements in the z dimension are not analyzed. Spine motility is defined as the average change in length per unit time (micrometers per minute). Lengths are measured from the base of the protrusion to its tip. The position of spines are compared on different imaging days. Spines that are farther than 0.5 μm laterally from their previous location are considered to be different spines. Values for stable spines are defined as the percentage of the original spine population present on the second day of imaging. Only areas that show high signal-to-noise ratio in all imaging sessions will be considered for analysis. Analysis is performed blind with respect to animal age and sensory cortical area. Spine motility (e.g.; spine turnover), morphology, and density are then compared between control and treatment groups. It is expected that treatment with a compound disclosed herein will rescue defective spine morphology relative to that observed in untreated control animals.

Example 296 Treatment of Clinical Depression by Administration of a PAK Inhibitor Compound Disclosed Herein in an Animal Model

A rat olfactory bulbectomy (OBX) model of clinical depression (see, e.g., van Riezen et al (1990), Pharmacol Ther, 47(1):21-34; and Jarosik et al (2007), Exp Neurol, 204(1):20-28) is used to evaluate treatment of clinical depression with a compound disclosed herein. Dendritic spine density and morphology are compared in treated and untreated groups of animals as described below. It is expected that treatment of OBX animals with a PAK inhibitor will cause an increase in spine density relative to that observed in untreated OBX animals.

All experiments are performed in strict accordance with NIH standards for laboratory animal use. The study uses 48 adult male Sprague-Dawley rats (230-280 g) housed in groups of four animals (two sham and two OBX), as indicated in van Riezen et al supra, in a controlled environment with food and water available ad libitum. Half of the experimental animals (n=24) undergo bilateral olfactory bulbectomy (OBX) while the other half undergo sham surgery (n=24). Upon completion of surgery, animals are allowed to recover for 2 weeks prior to behavioral testing. This is necessary to: 1) allow for the recovery of animal body weight which is reduced following surgery, 2) allow complete healing of superficial surgical sites, and) “bulbectomy syndrome” develops during the first 2 weeks postsurgery.

Two weeks after surgery, OBX and sham-operated animals are subdivided into one of four experimental conditions. One group of OBX animals is administered daily injections of saline solution (n=6 for each surgical condition) or compound disclosed herein (1 mg/kg; oral gavage) (n=6 for each surgical condition). These groups are included to examine the effect of chronic administration of compound disclosed herein (PAK inhibitor) on olfactory bulbectomized animals (2 weeks postsurgical recovery+2 weeks PAK inhibitor treatment). Administration of the drug or control solution are given at the same time each day and in the home cage of each animal. Groups of OBX and sham-operated animals receive no treatment during this 2-week period and serve as unhandled controls. These groups are necessary to examine the persistence of observed effects of OBX on dendritic spine density (4 weeks postsurgery). Animals receiving postsurgery drug treatment are sacrificed 24 h after the last injection.

Animals are perfused transcardially with 4% formaldehyde (in 0.1 M sodium phosphate buffer, pH=7.4) under deep anesthesia with sodium pentobarbital (60 mg/kg) at the completion of experimental procedures. Following fixation, brains are removed and placed in 4% formaldehyde (freshly depolymerized from para-formaldehyde) overnight. Brains are then sectioned at 100 μm on a vibratome and prepared for Golgi impregnation using a protocol adapted from previously described methods (Izzo et al, 1987). In brief, tissue sections are postfixed in 1% OsO4 for 30 min and then washed in 0.1 M phosphate buffer (3×15 min). Sections are free-floated in 3.5% K2Cr2O7 solution for 90 min, mounted between two microscope slides in a “sandwich” assembly, and rapidly immersed in a 1% AgNO3 solution. The following day, sections are rinsed in ddH 2O, dehydrated in 70% and 100% ethanol, cleared with Histoclear™, and mounted on microscope slides with DPX.

Dendritic spines are counted on 1250× camera lucida images that include all spines observable in each focal plane occupied by the dendrite. Cells are analyzed only if they are fully impregnated (CAL primary apical dendrites extended into stratum lacunosum moleculare and basilar dendrites extended into stratum oriens; CA3: primary apical dendrites extended into stratum lacunosum moleculare and basilar dendrites extended into stratum oriens; dentate gyrus: secondary dendrites extended from primary dendrite within the molecular layer), intact, and occurring in regions of the section that are free of blood vessels, precipitate, and/or other imperfections. Dendritic spines are counted along the entire length of secondary oblique dendritic processes (50-100 μm) extending from the primary apical dendrite within stratum radiatum of area CA1 and CA3. In CA1 and CA3, secondary dendrites are defined as those branches projecting directly from the primary apical dendrite exclusive of tertiary daughter branches. In addition, spines are counted along the length of secondary dendrites of granule cells in the dentate gyrus to determine if effects are limited to CA1 and CA3. In dentate gyrus, secondary dendrites are analyzed in the glutamatergic entorhinal input zone in the outer two-thirds of the molecular layer. Approximately 20 dendritic segments (10 in each cerebral hemisphere; 50-100 μm in length) in each hippocampal subregion (CA1, CA3, and dentate gyrus) are examined for each experimental animal. Treatment conditions are coded throughout the entire process of cell identification, spine counting, dendritic length analysis, and subsequent data analysis. Analysis of variance and Tukey post-hoc pairwise comparisons are used to assess differences between experimental groups.

When significant changes in dendritic spine density are observed, camera lucida images and the Zeiss CLSM measurement program are used to quantify the number and length of secondary dendrites. This analysis is necessary as apparent changes in dendritic spine density can result from an increase or decrease in the length of dendrites and not the formation or loss of spines per se. Photomicrographs are obtained with a helium-neon 633 laser and Zeiss 410 confocal laser scanning microscope.

Example 297 Treatment of Epilepsy by Administration of a PAK Inhibitor Compound Disclosed Herein in an Animal Model

A rat tetanus toxin model of epilepsy is used to evaluate treatment of epilepsy with compound disclosed herein.

Wistar rat pups (Harlan Sprague Dawley, Indianapolis, Ind.), 10 d of age, are anesthetized with an intraperitoneal injection of ketamine and xylazine (33 and 1.5 mg/kg, respectively). When necessary, this is supplemented by inhalation of methoxyflurane (Metofane). Tetanus toxin solution to be injected is generated by dissolving 2.5 or 5 ng of tetanus toxin in 20 or 40 nl of sterile saline solution. Afterwards, the tetanus toxin solution is coinjected into the right hippocampus along with a solution of a compound disclosed herein.

To inject tetanus toxin and a compound disclosed herein, the pups are placed in an infant rat stereotaxic head holder, a midline incision is made, and a small hole is drilled in the skull. The stereotaxic coordinates for injection are: anteroposterior, −2.1 mm; mediolateral, 3.0 mm from the bregma; and dorsoventral, −2.95 mm from the dural surface. The toxin and a compound disclosed herein are slowly injected at 4 nl/min. After injection, the needle is left in place for 15 min to reduce reflux up the needle track. During injections, the body temperature of rat pups is maintained by a warmed (electrically regulated) metal plate. Littermates, stereotaxically injected with sterile saline, or untreated rats serve as controls.

The frequency of behavioral seizures is monitored for 1 hr/day for 10 consecutive days after tetanus toxin/the test compound injections. The types and duration of seizures are scored. Wild running seizures are most easily identified.

After seizure scoring on the 10th day animals are perfused transcardially and dendritic spines in the CA3 region are counted and analyzed as described above.

The t test for comparison of two independent means is used in comparing the number of seizures in treated vs. untreated rats and in comparing dendritic and axon arbors in experimental and control rats. When data are not normally distributed, a Mann-Whitney U test is used. Sigma Stat is used to perform all statistical tests. It is expected that treatment with a compound disclosed herein will reduce the frequency and severity of seizures.

Example 298 Treatment of Mild Cognitive Impairment by Administration of a PAK Inhibitor in an Animal Model

The ability of a compound of Formula I-XV to delay or halt the progression of symptoms of Mild Cognitive Impairment (i.e., their mouse analogs) is tested in a Tg2576 mouse model of Mild Cognitive Impairment (Young et al. (2009), Neurobiology of Aging, 30:1430-1443).

Thirty-two Tg2576 male mice (ages 3-4 months) and their wild-type littermates (n=8) are divided into a treatment groups (1 mg/kg oral gavage), placebo groups (0.1% DMSO in physiological saline solution) and wild-type and analyzed for behavioral differences in olfactory discrimination and odor recognition memory using a mouse odor span task apparatus (Young et al. (2007), Neuropharmacology 52:3634-645).

In each mouse odor span task test, a mouse is placed on an elevated wooden platform (61 cm×61 cm) using numbers as location identifiers. Numbers 1-24 are used, with 1, 7, 13, and 19 at each corner and the intervening five numbers evenly spaced between the corners locations. The following odors are used: allspice, Chinese five spice, cinnamon, nutmeg, coriander, fenugreek, ginger, paprika, thyme, parsley, dill, oregano, sage, mint, rosemary, onion powder, caraway seed, celery salt, cocoa, coffee powder (Maxwell House®), and English breakfast tea (Twinnings®). All scented mixtures are created by adding 3 g of a specific odor to 100 g of woodchip and 18 crushed food pellets (Noyes Precision Pellets, Lancaster, UK). These mixtures are placed in white porcelain bowls (5.5 cm in diameter, 3.5 cm high; Fisher Loughborough, UK) and are marked with a letter of the alphabet (A-v) identifying the odor.

After the mice are introduced to each odor, the odor span task tests are habituated to the testing protocol. Habituation is conducted as follows: Span 0: a bowl is baited and placed on the platform at the chosen location; with the introduction of the mouse (which always faces the experimenter's left; location 16) a timer is started. Digging in the bowl for the food pellet (reward) stops the timer and the mouse is required to remember the odor in that bowl. Following consumption of the reward, the mouse is removed to a clear Perspex cage located below the platform, a new bowl and location is selected, the bowl is baited and placed appropriately. The first bowl (no longer baited) is moved to a new location. Span 1: the mouse is placed back on the platform and the timer is restarted, with the mouse required to dig only in the novel bowl. After digging in either bowl the timer is stopped, and if a correct choice is made, the mouse is given time to consume the reward before being returned to the clear cage. The accuracy of this span is noted, for once the non-match rule is acquired this gave an indication of the ability of the mouse to perform a simple two-odor discrimination. Span 2: a third (baited) bowl is then placed on the platform in the designated location and the two previously sampled bowls are repositioned as required. If an incorrect response is made (digging in a previously sampled bowl), the three bowls are randomly relocated and the span is repeated until a correct response is made. The span number is then increased with every correct response until span 21 (22 bowls) is completed or the mouse has spent 10 min on the platform. Any incorrect response will lead to a repetition of that span with all bowls being randomly relocated.

The number of odors (bowls) a mouse remembers prior to erring is regarded as the mouse's span length for that session. The total number of spans completed is also recorded as are errors per session and % accuracy [(spans completed/spans completed+errors)×100]. Each subject's mean span latency (total correct latency/spans completed) is also calculated, with time to first sample (latency to complete span 0) being recorded to ensure that mice takes a comparable amount of time to engage in the task. A bowl is randomly selected every third span (spans 2, 5, 8 and 11) and replaced with an identical yet previously non-sampled odor filled bowl, which will unmask any scent marking strategy. In addition, between every session the table is wiped down with ethanol. The mice are continuously trained until a stable level of performance is reached, with performance then being assessed over 4 consecutive days.

The odor span task test is conducted at 4 months, 8 months and 12 months to evaluate the progression of Mild Cognitive Impairment in the Tg2576 mice. In this test, a significant increase in Span Length, a significant increase in % Accuracy, or significant decrease in errors per session over the course of the experimental period (e.g., results at 4 month vs. 8 months, results at 4 month vs. 8 months) in the test compound groups relative to the placebo group (and/or as compare to the wild-type group) is indicative of a successful treatment effect.

Statistical Analysis. Statistical analysis is performed by ANOVA or repeated ANOVA. Differences between groups are considered significant at p<0.05.

Example 299 Treatment of Mild Cognitive Impairment by Administration of a PAK Inhibitor in an Animal Model

The ability of compounds of Formula I-V to delay or halt the progression of behavioral symptoms and anatomical symptoms of Mild Cognitive Impairment (i.e., their mouse analogs) is tested in a Mo/Hu APP695swe mouse model of Alzheimer's disease (Knafo et al (2007), Cerebral Cortex Advance Access, Jul. 28, 2008).

Forty Mo/Hu APP695swe mice (ages 3 months) are divided into treatment groups (1 mg/kg oral gavage) and a placebo group (0.1% DMSO in physiological saline solution) and analyzed for memory differences in open field, prepulse inhibition, and hidden food behavioral tests, with an interval of about one week between each type of test. Each series of open field, prepulse inhibition, and hidden food behavioral tests are conducted at 3 months, 6 months, 9 months, and 12 months to evaluate the progression of cognitive impairment in the APP695swe mice.

In the open field test, each mouse is placed in a novel open field box (40 cm×40 cm; San Diego Instruments, San Diego, Calif.) for two hours. Horizontal and vertical locomotor activities in the periphery as well as the center area are automatically recorded by an infrared activity monitor (San Diego Instruments). Single breaks are reported as “counts.” In this behavioral test, a significant reduction in total activity in the test groups relative to the placebo group over the course of the testing period indicates a possible treatment effect.

In the hidden food test, mice are food-deprived for 24 h. After habituation to a new cage for 5 min, a food pellet is hidden under the cage bedding. The time it takes for the mouse to find the food pellet is measured until a maximum of 10 min is reached. In this behavioral test, a significant reduction in time to find the food pellet in the test groups relative to the placebo group over the course of the testing period is indicative of a successful treatment effect.

In the Morris Water Maze test, mice are placed in a pool with an exit platform. When released, the mouse swims around the pool in search of an exit while various parameters are recorded, including the time spent in each quadrant of the pool, the time taken to reach the platform (latency), and total distance traveled. The animal's ability to quickly find the platform, and on subsequent trials (with the platform in the same position) the ability to locate the platform more rapidly is recorded. Any significant showing of a reduced progression of the decline in performance in the test groups relative to the placebo group over the course of the testing period is indicative of a successful treatment effect.

The radial arm maze test, measures spatial learning and memory in mice. Mice are placed in an apparatus comprising eight equidistantly-spaced arms, each about 4 feet long, and all radiating from a small circular central platform. Food is placed at the end of each arm. The design ensures that, after checking for food at the end of each arm, the mouse is always forced to return to the central platform before making another choice. The ability of mice to remember locations on the arm is measured to determine memory and spatial learning. A significant showing of reduced progression in the decline of performance in the test groups relative to the placebo group over the course of the testing period is indicative of a successful treatment effect.

The T-maze is designed to test spatial working memory to assess hippocampal and forebrain function. In the “delayed non-match to place” or “delayed alternation” test, there are 2 runs per trial. On the first, or sample run, the mouse is placed in the start arm of the T-maze and allowed to enter a goal arm. The mouse is then removed from the maze for a specified delay period. After the delay, the mouse is returned for the choice run. The choice of arm used by the mouse is scored according to variety of criterion, including spontaneous alternation, cued reward, or to indicate a preference. Based on the criterion used in an experiment, the T-maze can be used to test learning and memory, preferences for stimuli or reward, or spontaneous alternation behavior. A significant showing of reduced progression in the decline of performance in the test groups relative to the placebo group over the course of the testing period is indicative of a successful treatment effect.

In the prepulse inhibition test, acoustic startle and prepulse inhibition responses are measured in a startle chamber (San Diego Instruments). Each mouse is individualed to six sets of seven trail types distributed pseudorandomly: pulse-alone trials, prepulse-pulse trials, and no-stimulus trials. The pulse used is 120 dB and the prepulse is 74 dB. A significant showing of reduced progression in the decline of the prepulse inhibition response in the test groups relative to the placebo group over the course of the testing period is indicative of a successful treatment effect.

In the forced swim test, each mouse is put in a large plastic cylinder, which is half-filled with room temperature water. The test duration is 6 min, during which the swim/immobility times are recorded. In this behavioral test, a significant showing of reduced progression in the decline of immobility in the test groups relative to the placebo group over the course of the testing period is indicative of a successful treatment effect.

In order to evaluate the ability of the test compounds to alter brain morphology, an MRI study is conducted on placebo-treated and test compound-treated groups of Mo/Hu APP695swe mice. In vivo MRI experiments are performed on an 11.7T Bruker Biospec small animal imaging system. A three-dimensional, fast-spin echo, diffusion weighted (DW) imaging sequence with twin navigation echoes is used to assess the ratio of lateral ventricle volume to total brain volume. A decrease in this ratio in the test compound-treated groups relative to the ratio observed in the placebo-group is indicative of a successful treatment effect.

Statistical Analysis. Statistical analysis is performed by ANOVA or repeated ANOVA. Differences between groups are considered significant at p<0.05.

Example 300 Treatment of Autism by Administration of a PAK Inhibitor in an Animal Model

The ability of a compound of Formula I-XV described herein (a PAK inhibitor) to alleviate, reduce the severity of, or inhibit the progression of symptoms of autism (i.e., their mouse analogs) is tested in a FMR1 KO mouse model.

Twenty-four FMR1 KO male mice (age 2 months) are divided into Group 1 (n=6) and Group 2 (n=6) treatment groups (1 mg/kg oral gavage of a compound of Formula I-V described herein), a placebo Group (Group 3) (n=6) (0.1% DMSO in physiological saline solution) and wild-type (Group 4) (n=6) and are analyzed for behavioral differences using the Open Field Test.

Open Field Test. The mice in Groups 1-4 are subjected to the open field test according to standard procedures. Each of the mice ran for 60 minutes in a VersaMax activity monitor chamber (Accuscan Instruments). Open field activity is detected by photobeam breaks and is analyzed by the VersaMax software. Stereotypy is recorded when the mouse breaks the same beam (or set of beams) repeatedly. Stereotypy count is the number of beam breaks that occur during this period of stereotypic activity.

FMR1 KO mice are known to exhibit three abnormal behaviors compared to wild-type mice (Peier et., 2000, Hum. Mol. Genet., 9:1145): (i) hyperactivity—they travel a longer distance and move for a longer period of time than wild-type; (ii) stereotypy—they exhibit a higher number of repetitive behaviors than wild-type; and (iii) hypo-anxiety—they stay in the center field for a longer period of time and in the corners of the field for shorter periods of time than wild-type.

It is expected that the FMR1 mice in treatment Group 1 and treatment Group 2 will perform comparable to the wild-type controls (Group 4) for: (i) hyperactivity; (ii) stereotypy; and (iii) hypo-anxiety as measured in the Open Field Test, whereas the FMR1 mice in Group 3 will exhibit abnormal behavior. This indicates that treatment of FMR1 KO mice with PAK inhibitors of a compound of Formula I-XV described herein restores activity, repetitive behavior, and anxiety to wild-type levels.

Statistical Analysis. Statistical analysis is performed by ANOVA or repeated ANOVA. Differences between groups are considered significant at p<0.05.

Example 301 Treatment of Autism by Administration of a PAK Inhibitor in an Animal Model

The ability of a compound of Formula I-XV described herein (a PAK inhibitor) to delay or halt the progression of behavioral symptoms of autism (i.e., their mouse analogs) is tested in a BTBR T1tfj mouse model of autism syndrome (McFarlane et al., Genes, brain, and behavior (2007)).

BTBR T1tfJ is an inbred mouse strain that shows robust behavioral phenotypes with analogies to all three of the diagnostic symptoms of autism, including well-replicated deficits in reciprocal social interactions and social approach, unusual patterns of ultrasonic vocalization, and high levels of repetitive self-grooming.

Twenty BTBR T1tfJ male mice (age 2 months) are divided into Group 1 (n=5) and Group 2 (n=5) treatment groups (1 mg/kg oral gavage of a compound of Formula I-V described herein), a placebo Group (Group 3) (n=5) (0.1% DMSO in physiological saline solution) and wild-type (Group 4) (n=5) and are analyzed for behavioral differences using the sociability test and self grooming test described below.

Sociability Test. Social approach behaviors are tested in an automated 3-chambered apparatus using methods similar to those previously described (Moy et al., 2004; Nadler et al., 2004; Crawley et al., 2007; McFarlane et al., 2007; Moy et al., 2007). Briefly, the apparatus is a rectangular, three-chambered box made from clear polycarbonate. Retractable doorways built in the two dividing walls allow access to the side chambers. Quantification of entries and duration in the chambers is automatically measured by photocells embedded in the doorways. The apparatus is cleaned with 70% ethanol and water between subjects.

Animals to be used as “strangers” are male 129Sv/ImJ and AJ mice, aged 8-14 weeks old (The Jackson Laboratory (Bar Harbor, Me.)). Strangers are habituated to the apparatus and to the wire cup enclosure before the start of experiments, for 10 min per day for three consecutive days. The subject mouse is allowed to acclimate to the apparatus for 20 min before the sociability test, 10 min in the central chamber with the doors closed and another 10 min in the entire empty arena with the doors open. The subject is then briefly confined to the center chamber while a novel object (inverted wire cup, Galaxy Cup) is introduced into one of the side chambers. A stranger mouse enclosed in an identical wire cup is placed in the other side chamber. An upright plastic drinking cup, held in place by a lead weight in the cup, is placed on the top of each inverted wire cup to prevent the subject from climbing onto the top of the wire cup. The location for the novel object and the stranger mouse alternates between the left and right chambers across subjects. After both stimuli are positioned, the doors are simultaneously re-opened and the subject is allowed access to all three chambers for 10 min. Measures to be taken include time spent in each chamber, time spent sniffing each cup, and number of entries. An observer uninformed of the genotypes scores time spent sniffing with a stopwatch.

Self-Grooming. The test is performed as previously described (McFarlane et al., 2007). Each subject is placed individually in a clean standard mouse cage and allowed to acclimate for 10 min. Following this habituation period, subjects are observed for another 10 min, during which time cumulative time spent in self-grooming is scored by an experimenter sitting approximately 2 meters from the test cage. A silenced stopwatch is used for scoring cumulative time spent grooming during the 10 min test session.

It is expected that the BTBR T1tfJ mice in treatment Group 1 and treatment Group 2 will perform comparable to the wild-type controls (Group 4) for: (i) sociability and (ii) self-grooming, whereas the BTBR T1tfJ mice in Group 3 will exhibit abnormal behavior. This indicates that treatment of BTBR T1tfJ mice with PAK inhibitors of a compound of Formula I-XV described herein restores low sociability and repetitive self-grooming behavior to wild-type levels.

Statistical Analysis. Statistical analysis is performed by ANOVA or repeated ANOVA. Differences between groups are considered significant at p<0.05.

Example 302 Treatment of Learning Deficits Associated with Neurofibromatosis Type 1 by Administration of a PAK Inhibitor in an Animal Model

Neurofibromatosis Type 1 (NF1) is one of the most common single-gene disorders that causes learning deficits in humans. Mice carrying a heterozygous null mutation of the Nfl gene (Nfl^(+/−)) show important features of the learning deficits associated with NFL

Generation of different genetically modified mice are described in Johnson, L. K-r. et al., Genes Dev. 11, 2468-81 (1997); Jacks, T. et al., Nature Genet. 7, 353-61 (1994); and Umanoff, H., Edelmann, W., Pellicer, A. & Kucherlapati, R., Proc. Natl. Acad. Sci. USA, 92, 1709-13 (1995).

Water Maze Experiment:

The protocol for the water maze experiment is described in Costa, R. M. et al., Nature Genet. 27, 399-405 (2001). Mice from the are given two trials per day (30-s intertrial intervals) with a probe trial (60 s) at the end of training day 7. In the probe trial, WT mice spent significantly more time in the training quadrant compared to Nfl^(+/−) animals. The PAK inhibitor test compound is dissolved in sterile saline solution and injected every day for several days (typical dosing regimen are 2 to 5 days of dosing). The Water Maze experiment is performed between 2 and 8 hours following the final dose.

Electrophysiology:

For field potentials, recordings are made from transverse hippocampal slices (400 μm thick) in a submerged recording chamber perfused (2 ml min⁻¹) with artificial cerebrospinal fluid (ACSF) containing (in mM): 120 NaCl, 3.5 KCl, 2.5 CaCl₂, 1.3 Mg₂SO₄, 1.25 NaH₂PO₄, 26 NaHCO₃, and 10 D-glucose at 30 deg. C. (saturated with 95% O2 and 5% CO₂). For LTP experiments, EPSPs are evoked alternatively in separate pathways (control and tetanized) in a CA1 Schaffer collateral/commissural afferents with 100-μs test pulses through two stimulating electrodes (about 300 mm from the Pt/Ir recording electrode. The stimulation strength in both stimulating electrodes is set to 60 μA. After a 10-min baseline period, LTP is induced in one pathway according to a HFS or TBS protocol. The amount of potentiation is calculated as a percentage of the baseline EPSP slope.

To access inhibition in Nfl^(+/−) mice, IPSPs from CA1 pyramidal neurons are measured using whole-cell (blind technique) bridge mode recordings (Axoclamp 2B, Axon Instruments). IPSPs are evoked through a stimulating electrode placed in the Schaffer collateral/commissural afferents from applying different stimulation strengths (from 10 to 100 μA in steps of 10 μA). The IPSP amplitude is measured with five IPSPs averaged for each neuron per stimulation strength. The intracellular solution contains (in mM): 135 potassium gluconate, 5 HEPES, 2 Mg²⁺-ATP, 5 MgCl₂, 0.3 GTP, 0.05 EGTA. To evoke IPSPs monosynaptically, AP5 and CNQX (10 μM) are present in the ACSF.

Statistical Analysis: Acquisition data from the water maze are analyzed by repeated-measures ANOVA. Percent time in training quadrant for the different genotypes are analyzed using single factor ANOVA; post-hoc comparisons between genotypes are carried out when appropriate. Planned comparisons using a paired t-test are used to analyze the proximity data. LTP is analyzed using single factor ANOVA on the average amount of LTP 30-40 min after induction. Inhibition and input-output curves are analyzed using ANOVA and post-hoc comparisons are performed when appropriate.

Example 303 Clinical Trial: Treatment of Schizophrenia with a PAK Inhibitor Compound Disclosed Herein

The following human clinical trial is performed to determine the safety and efficacy of a PAK inhibitor compound for the treatment of schizophrenia.

Sixty patients are recruited via referrals from community mental health teams, after the patients have been diagnosed with schizophrenia using the Structured Clinical Interview for DSM-IV (“SCID”; First et al., (1995), Structured Clinical Interview for DSM-IV Axis I Disorders, Patient Edition (SCID-P), version 2, New York State Psychiatric Institute, Biometrics Research, New York).

A screening visit is arranged and a full explanation of the study prior to screening is provided if the patient appeared suitable for and interested in taking part. For inclusion, all patients are required to meet the following criteria: (i) aged between 18 and 60 years, (ii) receiving stable treatment with an atypical (Risperidone, Olanzapine, Quetiapine) antipsychotic and have stable psychotic symptoms (i.e. no change in medication/dose of current medication over last 6 weeks and unlikely to require change in antipsychotic medication), (iii) negative urine screening for illicit drugs and negative pregnancy test for female patients, (iv) cooperative, able to ingest oral medication and willing to undertake repeated cognitive testing, (v) able to provide written informed consent, (vi) reading ability of not more than 40 errors on the National Adult Reading (Nelson et al, (1991)), and (vii) between 1 and 2 standard deviations (S.D.) below expected performance on the basis of age and education level on the California Verbal Learning Test (Delis et al., 1987). In addition, the following criteria are used to define unsuitable patients: (i) concurrent DSM-IV diagnosis, (ii) current treatment with benzodiazepines or antidepressants, (iii) history of neurodegenerative disorder in first degree relative (e.g. AD, Parkinson's disease, Huntington's disease, multiple sclerosis), (iv) history of DSM-IV substance dependence in the last year or substance abuse within last month, (v) lifetime history of trauma resulting in loss of consciousness for 1 h or longer, (vi) participation in another investigational drug trial within 6 weeks prior to study entry, (vii) recent (within last 3 months) history of suicidal or violent acts, and (viii) current diagnosis of uncontrollable seizure disorder, active peptic ulceration, severe and unstable cardiovascular disease or/and acute severe unstable asthma. The study procedures are approved by an institutional ethics review board. All patients in the study must provide written informed consent.

After screening has identified suitable patients that have provided informed consent, patients are placed on a single-blind placebo for 1 week. After 1 week on placebo (baseline), all patients complete a comprehensive cognitive test battery and undergo clinical assessments, and then are randomized into a double-blind protocol so that, half of the sample received a compound disclosed herein capsules and the remaining half received placebo for the next 24 weeks. Cognitive and clinical assessments are carried out again at 12 weeks and 24 weeks.

Patients assigned to the treatment group will receive 1.5 mg twice a day for the first 2 weeks, 3 mg twice a day over the next 2 weeks, 4.5 mg twice a day dose for the next 2 weeks and then 6 mg twice a day for the remaining period so at the time of 12 weeks cognitive assessments all patients are on the maximum dose. The placebo group will receive identical appearing capsules containing ascorbic acid (100 mg).

Symptoms are rated within 4 days of cognitive testing using the Positive and Negative Syndrome scale (PANSS) (Kay et al. (1987), Schizophr Res, 13:261-276) on all three occasions. Side effects are also assessed within 4 days of testing using the Abnormal Involuntary Movement Scale (AIMS) (Guy, (1976), ECCDEU Assessment Manual for Psychopharmacology (revised), DREW Publication No. (ADM) National Institutes of Mental Health, Rockville, Md., pages 76-338). Inter-rater reliability is carried out for PANSS at 6 monthly intervals by rating exemplar cases based on patient interviews on videotapes.

The cognitive battery includes measures of executive functioning, verbal skills, verbal and spatial working memory, attention and psychomotor speed. The battery is administered to all patients on all three occasions in the same fixed order (e.g., MATRICS cognitive battery, BACS score, and performance in Wisconsin Card Sort Test). Patients are allowed to take breaks as needed in order to obtain maximal performance at all times. Tests are administered and scored by trained psychologists who are blind to patients' group affiliations and are not involved in patients' treatment plan in any way.

Patients are told that the aim of the study is to investigate the cognitive effects of a compound disclosed herein. They are requested to abstain from alcohol for at least 24 h prior to their scheduled cognitive testing.

The patients in the treatment and placebo groups are compared on demographic, clinical, and cognitive variables obtained at baseline using independent sample I-tests.

The effects of the test compound on positive symptoms, negative symptoms, general psychopathology score, total PANSS scores, and the scores on the AIMS are analyzed (separately) by 2 (Treatment, placebo)×3 (Time: baseline, 12 weeks, 24 weeks) analysis of variance (ANOVA).

All cognitive variables are first examined for their distribution properties, i.e., to ensure normality. The cognitive effects of the test compound over time are then evaluated by Treatment×Time ANOVA, performed separately for each variable, with Time as a within-individuals factor and Treatment as a between-individuals factor, followed by post-hoc mean comparisons wherever appropriate. All cognitive effects are then re-evaluated using ANOVA performed separately on change scores computed for each variable (12 weeks data minus baseline data, 24 weeks data minus baseline data). Alpha level for testing significance of effects is p=0.05.

Example 304 Clinical Trial: Treatment of Epilepsy with a PAK1/PAK3 Inhibitor

This is a 24-week study of an oral PAK1/PAK3 inhibitor in symptomatic patients with a diagnosis of epilepsy. This is an open-label, single-arm study to evaluate the dosing, tolerability, effectiveness and safety of a PAK1/PAK3 inhibitor as initial therapy for epilepsy. A total of 30 subjects will enrolled in the study.

Study Type: Interventional

Primary Outcome Measures:

Comparison of the mean stabilized dose of a PAK1/PAK3 inhibitor during the last 28 days of treatment between patients reporting 1 to 3 seizures versus patients reporting more than 3 seizures, during the 3 months prior to study entry

Secondary Outcome Measures:

Influence of other patient characteristics on dose; Proportion of subjects remaining seizure-free; Time to stabilized dose; Reduction in seizure frequency.

Inclusion Criteria:

Subjects having new-onset epilepsy or epilepsy relapse characterized by partial-onset seizures or primary generalized tonic-clonic seizures; Having at least 1 seizure within the 3 months prior to entry; Subjects who are previously untreated for epilepsy, previously treated for epilepsy, or if currently taking epilepsy medication, must have been taking it for less than 6 weeks

Exclusion Criteria:

Subjects currently on any medication for epilepsy for greater than 6 weeks; Having active liver disease.

Experimental Design

Patients are divided into two groups, a placebo group and a PAK1/PAK3 inhibitor group. Patients are administered tablets starting at 50 milligrams per day and titrated to an individualized optimal dose, up to a maximum of 400 milligrams per day of the PAK1/PAK3 inhibitor by the end of week 6. Patients will take tablets by mouth twice a day (morning and evening) for 24 weeks. Changes to this schedule will be based on a risk-benefit assessment of the patient's clinical condition by the investigator, such as tolerability, or reaching a stable dose sufficient to control their seizures.

Patients are evaluated at weekly visits over a period of 6 weeks. Groups are compared using ANOVA. Single variable differences are analyzed using an independent samples t-test. A Pearson's coefficient is used to determine relationship between seizure frequency and medication dose.

Example 305 Clinical Trial: Treatment of Alzheimer's disease with a PAK Inhibitor

The following human clinical trial is performed to determine the safety and efficacy of the PAK inhibitor disclosed herein for the treatment of Alzheimer's disease. The study aims to provide preliminary estimates of effect of administration of a PAK inhibitor in delaying progression of disease over a study period of one year.

Sixty patients between the ages of 55 and 80 are recruited via referrals from hospitals, after the patients have been diagnosed with mid stage Alzheimer's disease using the Mini-Mental State Exam scores and a clinical interview.

A screening visit is arranged and a full explanation of the study prior to screening is provided if the patient appeared suitable for and interested in taking part. For inclusion, all patients are required to meet the following criteria: (i) diagnosis of Alzheimer's disease (ii) a study partner who can attend all study visits (iii) negative urine screening for illicit drugs (iv) cooperative, able to ingest oral medication and willing to undertake repeated cognitive testing, (v) able to provide written informed consent. Exclusion criteria include (i) significant neurological disease other than Alzheimer's disease (ii) significant depression or other psychiatric disorder (iii) unstable medical conditions. The study procedures are approved by an institutional ethics review board. All patients in the study must provide written informed consent.

After screening has identified suitable patients that have provided informed consent, patients are placed on a single-blind placebo for 1 week. After 1 week on placebo (baseline), all patients complete a comprehensive cognitive test battery and undergo clinical assessments, and then are randomized into a double-blind protocol so that, half of the sample received test compound capsules and the remaining half received placebo for the next 52 weeks. Cognitive and clinical assessments are carried out again at 12 weeks, 26 weeks and 52 weeks. Patients assigned to the test compound group will receive a dose twice a day for 12 weeks at increasing doses. Cognitive assessments for all patients are on the maximum dose. The placebo group will receive identical appearing capsules containing ascorbic acid (100 mg).

The cognitive battery includes measures of executive functioning, verbal skills, verbal and spatial working memory, attention and psychomotor speed. The battery is administered to all patients on all three occasions in the same fixed order (e.g., Mini-Mental State Examination (MMSE), MATRICS cognitive battery, BACS score, and Alzheimer's disease Assessment Scale-Cognitive Subscale (ADAS-Cog)). Patients are allowed to take breaks as needed in order to obtain maximal performance at all times. Tests are administered and scored by trained psychologists who are blind to patients' group affiliations and are not involved in patients' treatment plan in any way. Alzheimer's disease Cooperative Study-Activities of Daily Living (ADCS-ADL) is also recorded.

Patients are told that the aim of the study is to investigate the cognitive effects of the test compound. They are requested to abstain from alcohol for at least 24 h prior to their scheduled cognitive testing.

The patients in the test compound and placebo groups are compared on demographic, clinical, and cognitive variables obtained at baseline using independent sample I-tests.

The effects of the test compound on Neuropsychological Test Battery and Neuropsychiatric Inventory (NPI) are analyzed (separately) by 2 (Treatment: Test compound, placebo)×3 (Time: baseline, 12 weeks, 26 weeks, 52 weeks) analysis of variance (ANOVA).

All cognitive variables are first examined for their distribution properties, i.e., to ensure normality. The cognitive effects of test compound over time are then evaluated by Treatment×Time ANOVA, performed separately for each variable, with Time as a within-individuals factor and Treatment as a between-individuals factor, followed by post-hoc mean comparisons wherever appropriate. All cognitive effects are then re-evaluated using ANOVA performed separately on change scores computed for each variable (12 weeks data minus baseline data, 26 weeks, 52 weeks data minus baseline data). Alpha level for testing significance of effects is p=0.05.

Primary outcome measure is an improvement in (ADAS-Cog) scores. Secondary outcome measures are improvement in (MMSE) scores and (ADCS-ADL).

Example 306 Clinical Trial: Treatment of Mild Cognitive Impairment with a PAK Inhibitor

The following human clinical trial is performed to determine the safety and efficacy of the PAK inhibitor having the structure of Formula I-XV for the treatment of Mild Cognitive Impairment. The study aims to provide preliminary estimates of effect of administration of a PAK inhibitor in delaying progression of the disease over a study period of one year.

Sixty patients between the ages of 45 and 80 are recruited via referrals from hospitals, after the patients have been diagnosed with Mild Cognitive Impairment using the Mini-Mental State Exam scores (MMSE score of 21-24) and a clinical interview.

A screening visit is arranged and a full explanation of the study prior to screening is provided if the patient appeared suitable for and interested in taking part. For inclusion, all patients are required to meet the following criteria: (i) diagnosis of Mild Cognitive Impairment (ii) a study partner who can attend all study visits (iii) negative urine screening for illicit drugs (iv) cooperative, able to ingest oral medication and willing to undertake repeated cognitive testing, (v) able to provide written informed consent. Exclusion criteria include (i) significant neurological disease and/or dementia (including Alzheimer's disease) (ii) significant depression or other psychiatric disorder (iii) unstable medical conditions. The study procedures are approved by an institutional ethics review board. All patients in the study must provide written informed consent.

After screening has identified suitable patients that have provided informed consent, patients are placed on a single-blind placebo for 1 week. After 1 week on placebo (baseline), all patients complete a comprehensive cognitive test battery and undergo clinical assessments, and then are randomized into a double-blind protocol so that, half of the sample received test compound capsules and the remaining half received placebo for the next 52 weeks. Cognitive and clinical assessments are carried out again at 12 weeks, 26 weeks and 52 weeks.

Patients assigned to the test compound group will receive 1.5 mg twice a day for the first 2 weeks, 3 mg twice a day over the next 2 weeks, 4.5 mg twice a day dose for the next 2 weeks and then 6 mg twice a day for the remaining period so at the time of 12 weeks cognitive assessments all patients are on the maximum dose. The placebo group will receive identical appearing capsules containing ascorbic acid (100 mg).

The cognitive battery includes measures of executive functioning, verbal skills, verbal and spatial working memory, attention and psychomotor speed. The battery is administered to all patients on all three occasions in the same fixed order (e.g., Mini-Mental State Exam (MMSE), Wechsler Intelligence Scale, Wechsler Memory Scale, Dementia Rating Scale (DRS) or Auditory Verbal Learning Test (AVLT)). Patients are allowed to take breaks as needed in order to obtain maximal performance at all times. Tests are administered and scored by trained psychologists who are blind to patients' group affiliations and are not involved in patients' treatment plan in any way.

Patients are told that the aim of the study is to investigate the cognitive effects of a compound of Formula I-XV. They are requested to abstain from alcohol for at least 24 h prior to their scheduled cognitive testing.

The patients in the test compound group and placebo groups are compared on demographic, clinical, and cognitive variables obtained at baseline using independent sample I-tests.

The effects of test compound on Neuropsychological Test Battery and Neuropsychiatric Inventory (NPI) are analyzed (separately) by 2 (Treatment: test compound, placebo)×3 (Time: baseline, 12 weeks, 26 weeks, 52 weeks) analysis of variance (ANOVA).

All cognitive variables are first examined for their distribution properties, i.e., to ensure normality. The cognitive effects of the test compound(s) over time are then evaluated by Treatment×Time ANOVA, performed separately for each variable, with Time as a within-individuals factor and Treatment as a between-individuals factor, followed by post-hoc mean comparisons wherever appropriate. All cognitive effects are then re-evaluated using ANOVA performed separately on change scores computed for each variable (12 weeks data minus baseline data, 26 weeks, 52 weeks data minus baseline data). Alpha level for testing significance of effects is p=0.05.

Primary outcome measure is an improvement in MMSE scores. Secondary outcome measures are improvements in DRS scores and AVLT scores.

Example 307 Clinical Trial: Treatment of Amnestic Mild Cognitive Impairment with a Compound of Formula I-XV

This is a 40-week, randomized, double blind, parallel groups designed, study of an oral inhibitor having the structure of Formula I-XV in symptomatic patients with a diagnosis of amnestic Mild Cognitive Impairment. This pilot study aims to provide preliminary estimates of effect of an inhibitor having the structure of Formula I-XV on cognitive deficits and whether the effects differ between amnestic Mild Cognitive Impairment patients treated with an inhibitor, and amnestic Mild Cognitive Impairment patients treated with donepezil. A total of 30 subjects will enrolled in the study.

Study Type: Interventional

Study Design: Treatment, Randomized, Double Blind (Subject, Investigator), Active Control, Parallel Assignment, Efficacy Study

Primary Outcome Measures:

To provide preliminary estimates of dose of an inhibitor having the structure of Formula I-XV on cognitive deficits and difference between amnestic Mild Cognitive Impairment patients treated with the inhibitor, and amnestic Mild Cognitive Impairment patients treated with donepezil. Improvement in Mini-Mental State Exam (MMSE), Dementia Rating Scale (DRS) or Auditory Verbal Learning Test (AVLT) scores are the primary outcome measures of this study.

Secondary Outcome Measures:

To determine if the inhibitor having the structure of Formula I-XV has comparable or better efficacy for treating cognitive deficits of amnestic Mild Cognitive Impairment compared to efficacy of donepezil for treating cognitive deficits of amnestic Mild Cognitive Impairment.

Inclusion Criteria:

Subjects between ages 55-80, both males and females. Diagnosis of amnestic Mild Cognitive Impairment. Had a CT scan or MRI scan within the prior 12 months, which is compatible with a diagnosis of probable amnestic Mild Cognitive Impairment. Asymptomatic with regard to dementia. MMSE scores of 21-24.

Exclusion Criteria:

Significant neurological disease including Alzheimer's disease, cerebral tumor, Huntington's Disease, Parkinson's Disease, normal pressure hydrocephalus, or other diseases. Abnormal laboratory tests that might point to another etiology for dementia: serum B12, folate, thyroid functions, electrolytes, syphilis serology. Musculoskeletal diseases that could interfere with assessment. Use of any drug within 14 days prior to randomization unless the dose of the drug and the condition being treated have been stable for at least 30 days and are expected to remain stable during the study and neither the drug nor the condition being treated is expected to interfere with the study endpoints.

Experimental Design

Patients are divided into two groups, a donepezil group and a PAK1/PAK3 inhibitor group. Each patient receives two daily doses of donepezil or a PAK1/PAK3 inhibitor. Patients are monitored for a period of 40 weeks with experimental sessions every 4 weeks.

Subjects are seated in a chair for each experimental session that lasts about 3 h. Surface electromygraphy (EMG) is recorded from the right abductor pollicis brevis (APB) muscle with disposable disc electrodes placed in a tendon-belly arrangement over the bulk of the APB muscle and the first metacarpal-phalangeal joint. The EMG is monitored on a computer screen, the signal is amplified and stored in a laboratory computer for off-line analysis. Transcranial magnetic stimulation (TMS) is performed with a Magstim 200 stimulator placed at an optimal position on the APB muscle. Electric stimulation of the right median nerve is performed with a stimulation block using constant current square wave pulses with cathode positioned proximally. The stimulus intensity delivered is 300% of the sensory threshold.

Cortical excitability and cortical inhibition is measured prior to and after Paired Associative Stimulation (PAS). PAS consists of electric stimuli delivered to the right median nerve, paired with single pulse transcranial magnetic stimulation (TMS) over contralateral M1, with median nerve stimulation preceding TMS with interstimulus interval of 25 ms. Pairs of TMS and electrical stimuli are delivered at 0.1 hz over a 30 min period, reaching a total of 180 pairs. Cortical excitability is measured using motor evoked potentials (MEPs) size which is defined as intensity of stimulus sufficient to produce a mean MEP amplitude of 1 mV peak-to-peak response at baseline (stimulus intensity of SI_(1mV)). Cortical inhibition is measured using cortical silent period (CSP). The CSP duration is the time from MEP onset to return of voluntary EMG activity.

Patients are evaluated at weekly visits over a period of 40 weeks. Groups are compared using ANOVA. Single variable differences are analyzed using an independent samples t-test. A Pearson's coefficient is used to determine relationship between cognition and medication dose. Clinical Global Impressions (CGI) score, performance on Mini-Mental State Exam (MMSE), Dementia Rating Scale (DRS), Boston Naming Test, Stroop Color Word Test, Trail Making Test or Auditory Verbal Learning Test (AVLT) are scored at each visit. Clinician's Interview-Based Impression of Change are also recorded at each visit.

Example 308 Clinical Trial: Treatment of Autism with a PAK Inhibitor

The following human clinical trial is performed to determine the safety and efficacy of a PAK inhibitor compound of Formula I-XV described herein for the treatment of autistic spectrum disorders. The study aims to provide preliminary estimates of effect of administration of a PAK inhibitor (of Formula I-XV described herein) in alleviating, inhibiting the progression of, or reducing the severity of at least one behavioral symptom associated autistic spectrum disorders over a three month study period. Clinical observations of global function in language and/or behavior pattern are assessed.

Twenty-four patients, including 20 males and 4 females with an average age of 9 years and meeting DSM-IV criteria for ASD, are treated with a compound of Formula I-XV described herein for up to three months. Patients assigned to the Experimental group will receive 1.5 mg twice a day for the first 2 weeks, 3 mg twice a day over the next 2 weeks, 4.5 mg twice a day dose for the next 2 weeks and then 6 mg twice a day for the remaining period so at the time of the 12 weeks behavioral assessments, all patients are on the maximum dose.

The patients are evaluated using a global clinical improvement scale rating for improvement in language and behaviors based on parental observation and clinical appearance. Improvements are rated as follows: moderate to significant, mild to moderate, or no improvement.

After the twenty-four patients are treated for more up to three months with a compound of Formula I-XV described herein, parents report improvements in 20 of the 24 patients in one or more categories: attention, motor planning, language function (both receptively and expressively), and self-stimulatory behaviors.

Example 309 Clinical Trial to Evaluate the Safety of a Compound of Formula I-XV in Individuals with Neurofibromatosis Type I (NF1)

Purpose: Neurofibromatosis type I (NFI) is a genetic disorder that affects approximately 1 in 3500 individuals. Half of people with NF1 inherit the condition from a parent, and half have a new occurrence of the condition. The manifestation of NF1 is highly variable and multiple organ systems are typically affected. Some of the more common symptoms include benign neurofibromas, café au lait spots, Lisch nodules (tan spots on the iris of the eye). Some individuals with NF1 also exhibit more severe associated conditions, such as optic pathway tumors (gliomas) or bones bending or curving. Neurocognitive deficits and specific learning disabilities occur in approximately 30 to 50% of individuals with NF1 and are regarded by some observers and sufferers to be among the most troubling features of a disease. The most commonly reported findings are deficits in visuoperceptual ability, motor coordination, expressive and receptive language, and executive functioning, which requires intact short-term memory and attention. Patients with NF1 also show a slight depression in mean IQ scores compared to healthy adults without the disorder.

While cognitive deficits are now a widely-recognized feature of neurofibromatosis type I (NF1), the precise cause of these deficits still remain to be determined.

A randomized, double-blinded, placebo-controlled, trial of a compound of Formula I-XV in patients with NF1. Participants are randomly assigned to a compound of Formula I-XV or placebo and treated for approximately 14 weeks with baseline and follow-up assessments to evaluate safety and any effects on neurocognitive test performance.

Study Type: Interventional

Design: Placebo Control; Endpoint Classification: Safety and Efficacy study

Primary Outcome Measures: Non-verbal learning [Time Frame: 14 weeks]

Secondary Outcome Measures: attention [Time Frame: 14 weeks]; tolerability of medication [Time Frame: 14 weeks]

Estimated Enrollment: 50

Eligibility: 10 years to 45 years; genders eligible for study: both

Inclusion Criteria:

-   -   a. a diagnosis of NF1 by NIH criteria     -   b. between 10 and 45 years of age     -   c. no evidence of a comorbid neurological disorder (e.g.,         epilepsy, encephalitis)     -   d. not suffering from hypercholesterolemia based on self-report,         collateral information from physician, or initial medical workup         using National Cholesterol Education Program (NCEP, JAMA 2001),         guidelines accepted by the American College of Cardiology (ACC)         and the American Heart Association (AHA)     -   e. no mental retardation (i.e., IQ greater than 70)     -   f. no evidence of significant and habitual alcohol or drug abuse         or dependence     -   g. sufficient acculturation and fluency in the English language         to avoid invalidating research measures of thought, language,         and speech disorder, and verbal abilities

Exclusion Criteria:

-   -   h. comorbid neurological conditions     -   i. significant drug or alcohol abuse     -   j. non-fluency in English

Example 310 Clinical Trial for the Treatment of Neurofibromatosis Type 2 using a Compound Described Herein

Purpose:

The purpose of this trial is to assess the efficacy, safety, tolerability, biologic activity, and pharmacokinetics of a compound described herein in patients with neurofibromatosis Type 2.

Primary Outcome Measures:

Tumor response (complete and partial)

Secondary Outcome Measures:

Hearing response in patients (>10 dB)

Safety

May include volumetric assessment (MRI)

Eligibility:

Patients ≧18 years old, diagnosis of NF2, presence of vestibular schwannomas

Criteria

-   -   a. Inclusion Criteria:         -   1. Confirmed NF2 diagnosis, age ≧18 years         -   2. Vestibular schwannoma not amenable to surgery, or surgery             declined due to high risk for permanent complications         -   3. Progressive and significant hearing loss

Design

Patients dosed in 28 day cycles, cycles repeat every 28 days in the absence of disease progression or unacceptable toxicity.

Response assessed after cycles 1 and 2, every two cycles thereafter.

Eligible patients continue treatment until progression of disease or unacceptable toxicity.

Example 311 Growth Inhibition of a Compound of Formula I in Various Cancer Cell Lines

Methodology: 60 cell lines (CCRF-CEM, HL-60(TB), K-562, MOLT-4, RPMI-8226, SR, A549, EKVX, HOP-62, HOP-92, NCI-H226, NCI-H23, NCI-H322M, NCI-H460, NCI-H522, COLO 205, HCC-2998, HCT-116, HCT-15, HT29, KM12, SW-620, SF-268, SF-295, SF-539, SNB-19, SNB-75, U251, LOX IMV1, MALME-3M, M14, SK-MEL-2, SK-MEL-28, SK-MEL-5, UACC-257, UACC-62, IGR-OV1, OVCAR-3, OVCAR-4, OVCAR-5, OVCAR-8, SK− OV-3, 786-0, A498, ACHN, CAKI-1, RXF 393, SN12C, TK-10, UO-31, PC-3, DU-145, MCF₇, NCI/ADR-RES, MDA-MB-231, HS 578T, MDA-MB-435, MDA-MB-468, BT-549, and T-47D) are grown in RPMI-1640 medium with 10% FBS. Stock solutions of a test compound are prepared in DMSO. Concentrations of from about 0.001 μM to about 20 μM of each compound in RPM-1640 media are prepared. The test compound is added to wells containing 50 μL of cells and medium. A CellTiter-Glo (CTG) assay is carried out on the 0 hr plate to obtain a 0 hr count. Cells are exposed to the test compound for 72 hours. Following the exposure period, the plates are assayed using CTG. Luminescence is recorded on Synergy. A test compound described herein exhibited a GI₅₀ in various cell lines of less than about 1 μM.

Example 312 Treatment of Schwannomas by Administration of a PAK Inhibitor Compound Disclosed Herein in an Animal Model

The NF2 protein is absent in ˜100% of sporadic schwannomas. Thus, an NF2 deficient-schwanoma mouse model was generated and used to evaluate treatment of sporadic schwannomas with a PAK inhibitor compound disclosed herein.

Murine Nf2^(−/−) cells may be generated by various means. For example, NF2^(−/−) cells may be generated by administering an shRNA or siRNA targeting an Nf2 gene, thereby disrupting the Nf2 gene. In another example, NF2^(−/−) cells may be generated by using a Cre recombinase.

Nf2^(−/−) SC4 cells may be generated by using a Cre recombinase. Pure populations of embryonic SC4 cells were isolated from Nf 2^(loxP/loxP−) mice at about embryonic day 13.5. The cells were then infected in vitro with an adenovirus expressing Cre recombinase to allow excision of an exon in the Nf2 gene and thereby disruption of the Nf2 locus. The cells were injected in nude mice in the subcutaneous space in the flank, and the resulting tumor was harvested and dissociated. The resulting single-cell suspension was placed in culture to generate the first “line”. Nf 2^(−/−) SC4 cells were maintained in DMEM supplemented with 100 ng/mL recombinant human neuregulin-β1 (PeproTech) and 5 μmol/L forskolin (Invitrogen).

Nf2^(−/−) cells (e.g., Nf2^(−/−) SC4 control cells) were transduced by lentiviruses carrying pLuc-mCherry and sorted by FACS. Cells (2×10⁵ or 5×10⁴) were transplanted into the sciatic nerve sheath of NOD/SCID mice (6-8 weeks of age) by intraneural injection to generate schwannomas. Mice injected with Nf2−/− cells were treated with a vehicle control or a PAK inhibitor compound disclosed herein. Tumor progression was monitored weekly by bioluminescence imaging (BLI) according to the manufacturer's instructions on an IVIS-200 system. The mice were sacrificed and the tumors from the vehicle control treated and PAK inhibitor treated mice were measured. As shown in FIG. 5, the average tumor weight of the PAK inhibitor treated mice (e.g., PAK inhibitor compound disclosed in Example 84) was less than the average tumor weight of the vehicle control treated mice. This data clearly demonstrates the efficacy of a PAK inhibitor for the treatment of schwannomas.

Example 313 Growth Inhibition of Various NF2 Deficient Cells by Administration of a PAK Inhibitor Compound Disclosed Herein

NF2 deficient cell lines were cultured in the appropriate medium. Once enough cells have propagated, plates of all adherent lines were seeded at 5,000-10,000 cells/well (depending on cell line) in a total volume of 50 μL, and plates of all suspension lines at 10,000-20,000 cells/well (depending on cell line) in a total volume of 50 μL. Plates were placed in a humidified cell culture incubator overnight to allow adherent cells to attach.

10 mM stocks of each PAK inhibitor compound in DMSO were prepared. Dilutions were performed with media to provide 1% DMSO 2× stock solutions of each PAK inhibitor compound.

50 μL of all PAK inhibitor compound 2× stocks were added to appropriate wells already containing 50 μL of cells and medium to expose cells to the final concentrations of compounds required. 50 μL of media was added to media and cell control wells and 50 μL of mix to vehicle control wells. At the same time as drug exposure, a CTG (CellTiter-Glo Assay) assay was performed on the 0 hr plate to obtain a 0 hr count. Cells were exposed to PAK inhibitor compounds or a DMSO control for 72 hours. Following the 72 hour exposure period, all remaining plates were assayed using CTG.

At the end of the 72 hour exposure period, plates were removed for a CTG assay from 37° C., 5% CO2 incubator and place on the bench at room temperature for 30 mins. 100 μL CTG reagent was added to each well, mixed for 2 minutes, followed by a further 10 minute incubation at room temperature. Luminescence for each well was determined.

Growth inhibition percent was calculated using the following equation: (Growth %=(Sample Value−T0Ave)/(Max−T0Ave)*100).

Nf2^(−/−) SC4 cells were treated with a DMSO control or a PAK inhibitor compound (1 uM concentration for 72 hours) disclosed in Examples 33 and 84. As shown in FIG. 4, cells treated with the PAK inhibitor compounds disclosed in Examples 33 and 84 showed a reduction in cell number when compared to the DMSO control treated cells.

NF2^(−/−) Schwannoma cells were treated with a DMSO control or a PAK inhibitor compound (1 uM concentration) disclosed in Examples 101, 122, or 190. As shown in FIG. 6, cells treated with the PAK inhibitor compounds showed a reduction in cell number when compared to the DMSO control treated cells.

NF2^(−/−) mesothelioma cells (e.g., NCI-H226 cells) were treated with varying concentrations of a PAK inhibitor compound disclosed in Examples 33, 84, or 122. As shown in FIG. 7, as the concentration of the PAK inhibitor compound increased (0 μM-30 μM), the growth (%) of the cells reduced.

These data clearly demonstrates that a PAK inhibitor compound inhibits cell proliferation in NF2 deficient cells.

Example 314 Growth Inhibition of Various PAK1 Amplified Cells by Administration of a PAK Inhibitor Compound Disclosed Herein

The PAK1 amplified cell lines were cultured in the appropriate medium. Once enough cells have propagated, plates of all adherent lines were seeded at 5,000-10,000 cells/well (depending on cell line) in a total volume of 50 μL, and plates of all suspension lines at 10,000-20,000 cells/well (depending on cell line) in a total volume of 50 μL. Plates were placed in a humidified cell culture incubator overnight to allow adherent cells to attach.

PAK1 amplified cell lines were cultured in the appropriate medium. Once enough cells have propagated, plates of all adherent lines were seeded at 5,000-10,000 cells/well (depending on cell line) in a total volume of 50 μL, and plates of all suspension lines at 10,000-20,000 cells/well (depending on cell line) in a total volume of 50 pt. Plates were placed in a humidified cell culture incubator overnight to allow adherent cells to attach.

10 mM stocks of each PAK inhibitor compound in DMSO were prepared. Dilutions were performed with media to provide 1% DMSO 2× stock solutions of each PAK inhibitor compound.

50 μL of all PAK inhibitor compound 2× stocks were added to appropriate wells already containing 50 μL of cells and medium to expose cells to the final concentrations of compounds required. 50 μL of media was added to media and cell control wells and 50 μL of mix to vehicle control wells. At the same time as drug exposure, a CTG (CellTiter-Glo Assay) assay was performed on the 0 hr plate to obtain a 0 hr count. Cells were exposed to PAK inhibitor compounds or a DMSO control for 72 hours. Following the 72 hour exposure period, all remaining plates were assayed using CTG.

At the end of the 72 hour exposure period, plates were removed for a CTG assay from 37° C., 5% CO2 incubator and place on the bench at room temperature for 30 mins. 100 μL CTG reagent was added to each well, mixed for 2 minutes, followed by a further 10 minute incubation at room temperature. Luminescence for each well was determined.

Growth inhibition percent was calculated using the following equation: (Growth %=(Sample Value−T0Ave)/(Max−T0Ave)*100).

PAK1 amplified NSCLC cells (e.g., EBC-1 cells, NCI-H520 cells, SK-MES-1 cells) were contacted with varying concentrations of the PAK inhibitor compound (0 μM-30 μM) disclosed in Examples 33, 84, or 122. As shown in FIGS. 8 (EBC-1 cells), 9 (NCI-H520 cells), and 10 (SK-MES-1 cells), as the concentration of the PAK inhibitor compound increased, the growth (%) decreased.

These data clearly demonstrate that a PAK inhibitor disclosed herein inhibits cell proliferation in PAK1 amplified NSCLC cells.

Example 315 Generation of NF2^(−/−) Cancer Animal Models

Several tumors are characterized by a reduction or loss of NF2 gene expression or activity. Therefore, the generation of NF2−/− cancer animal models could be useful for analyzing and evaluating PAK inhibitor compounds for the treatment of tumors or cancers characterized by a reduction or loss of NF2 gene expression or activity.

NF2^(−/−) cells (e.g., Nf2^(−/−) SC4 control cells) were transduced by lentiviruses carrying pLuc-mCherry and sorted by FACS. Cells (2×10⁵ or 5×10⁴) were transplanted into the sciatic nerve sheath of NOD/SCID mice (6-8 weeks of age) by intraneural injection. Tumor progression was monitored weekly by bioluminescence imaging (BLI) according to the manufacturer's instructions on an IVIS-200 system.

Once sciatic nerve tumors produced appropriate bioluminescence intensity (approximately 7 days post-implant), tumors were treated with a PAK inhibitor compound as disclosed herein.

At various time points, treatment efficacy was determined. Treatment efficacy may be determined by a variety of ways which are well-known in the art. For example, determination of tumor size and/or weight were used to determine treatment efficacy. Methods to determine tumor size include, but are not limited to, whole-body imaging or use of calipers.

Example 316 Clinical Trial to Evaluate the Safety of a Compound Described Herein in Patients with Imatinib-Resistant Chronic Myelogenous Leukemia (CML)

Purpose:

The purpose of this trial is to assess the efficacy, safety, tolerability, biologic activity, and pharmacokinetics of a compound described herein in patients with one of the following conditions:

Imatinib failure only:imatinib-resistant or intolerant CML—Chronic Phase (CP)

Imatinib-resistant or intolerant CML—Accelerated Phase (AP)

Imatinib-resistant or intolerant CML—Blast Crisis (BC)

Primary Outcome Measures:

To determine the maximum tolerated dose (MTD) and dose-limiting toxicity (DLT) of a compound described herein as a single agent when administered as an oral once-daily and twice daily dose to adult patients with imatinib-resistant CML

To characterize the pharmacokinetic profile of a compound described herein in serum and, where samples are available, in tumor cells and normal hematopoietic cells

To evaluate the efficacy and safety of a compound described herein in patients with imatinib-resistant or intolerant CML-BC, imatinib-resistant or intolerant CML-AP and imatinib-resistant or intolerant CML-CP

Secondary Outcome Measures:

To assess changes during and after therapy in malignant cells taken from the bone marrow and/or blood

To evaluate the population pharmacokinetics of a compound described herein

To examine whether individual genetic variation in genes relating to drug metabolism, CML and the drug pathway confer differential response to a compound described herein

To identify gene expression patterns in tumor cells that are associated with treatment response to a compound escribed herein or that correlate with the severity or progression of CML

Eligibility:

All genders 18 years and older

Criteria

-   -   a. Inclusion Criteria:         -   i. Main inclusion criteria include:             -   1. Patients with CML in blast crisis, CML in accelerated                 phase defined as never in blast crisis phase, or CML in                 chronic phase defined as never been in blast crisis                 phase or accelerated phase who have: *developed                 progressive disease during therapy with at least 600 mg                 of imatinib per day, -OR- *patients with CML on imatinib                 therapy, at any dose, developing progressive disease and                 the presence of a genetic mutation likely to result in                 imatinib resistance -OR- *have developed an intolerance                 to imatinib             -   2. CML patients who have been treated with an                 investigational tyrosine kinase inhibitor who otherwise                 meet the definition of imatinib-resistance or                 intolerance are eligible             -   3. Written informed consent prior to any study                 procedures being performed     -   b. Exclusion Criteria:         -   i. Impaired cardiac function         -   ii. Patients with severe/chronic or uncontrolled medical             conditions (including but not limited to diabetes,             infections, GI impairment, CNS infiltration, liver and             kidney disease)         -   iii. Prior and concomitant use of certain medications             (including but not limited to warfarin, chemotherapy,             hematopoietic colony-stimulating growth factors, medications             that can affect electrocardiogram test results, other             investigational drugs)         -   iv. Women who are pregnant or breastfeeding         -   v. Patients with a history of another primary malignancy             that is currently clinically significant or currently             requires active intervention         -   vi. Patients unwilling to comply with the protocol         -   vii. Known diagnosis of human immunodeficiency virus (HIV)             infection

Design:

Patients are dosed in 28 day cycles, cycles repeat every 28 days in the absence of disease progression or unacceptable toxicity

Response assessed after cycles 1 and 2, every two cycles thereafter

Eligible patients continue treatment until progression of disease or unacceptable toxicity

Example 317 Clinical Study of a Compound Disclosed Herein and Tamoxifen in Patients that Did not Respond to Previous Tamoxifen Treatment

Purpose:

The purpose of this trial is to assess the efficacy, safety, tolerability, biologic activity, and pharmacokinetics of a compound described and tamoxifen in patients that did not respond to previous tamoxifen treatment

Primary Outcome Measures:

Tumor response (complete and partial)

Secondary Outcome Measures:

Time to progression; overall survival; safety

Changes in phosphorylation in tumor tissue of ER-Serl 18, ER-Ser305

Eligibility:

Postmenopausal women

Criteria

-   -   a. Inclusion Criteria:         -   1. Postmenopausal women with ER positive locally advanced or             metastatic breast cancer after documented recurrence or             progression on tamoxifen and PAK1 over-expression and/or             nuclear localization         -   2. Recurrence while on, or within 12 months of end of             treatment with tamoxifen         -   3. Progression while on tamoxifen for locally advanced or             metastatic breast cancer         -   4. PAK1 over-expression and/or nuclear localization

Example 318 Pharmaceutical Compositions Example 318a Parenteral Composition

To prepare a parenteral pharmaceutical composition suitable for administration by injection, 100 mg of a water-soluble salt of a compound of Formula I-XV is dissolved in DMSO and then mixed with 10 mL of 0.9% sterile saline. The mixture is incorporated into a dosage unit form suitable for administration by injection.

Example 318b Oral Composition

To prepare a pharmaceutical composition for oral delivery, 100 mg of a compound of Formula I-XV is mixed with 750 mg of starch. The mixture is incorporated into an oral dosage unit for, e.g., a hard gelatin capsule, which is suitable for oral administration.

Example 318c Sublingual (Hard Lozenge) Composition

To prepare a pharmaceutical composition for buccal delivery, such as a hard lozenge, mix 100 mg of a compound of Formula I-XV with 420 mg of powdered sugar mixed, with 1.6 mL of light corn syrup, 2.4 mL distilled water, and 0.42 mL mint extract. The mixture is gently blended and poured into a mold to form a lozenge suitable for buccal administration.

Example 318d Fast-Disintegrating Sublingual Tablet

A fast-disintegrating sublingual tablet is prepared by mixing 48.5% by weigh of a compound of Formula I-XV, 44.5% by weight of microcrystalline cellulose (KG-802), 5% by weight of low-substituted hydroxypropyl cellulose (50 μm), and 2% by weight of magnesium stearate. Tablets are prepared by direct compression (AAPS PharmSciTech. 2006; 7(2):E41). The total weight of the compressed tablets is maintained at 150 mg. The formulation is prepared by mixing the amount of compound of Formula I-XV with the total quantity of microcrystalline cellulose (MCC) and two-thirds of the quantity of low-substituted hydroxypropyl cellulose (L-HPC) by using a three dimensional manual mixer (Inversina®, Bioengineering AG, Switzerland) for 4.5 minutes. All of the magnesium stearate (MS) and the remaining one-third of the quantity of L-HPC are added 30 seconds before the end of mixing.

Example 318e Inhalation Composition

To prepare a pharmaceutical composition for inhalation delivery, 20 mg of a compound of Formula I-XV is mixed with 50 mg of anhydrous citric acid and 100 mL of 0.9% sodium chloride solution. The mixture is incorporated into an inhalation delivery unit, such as a nebulizer, which is suitable for inhalation administration.

Example 318f Rectal Gel Composition

To prepare a pharmaceutical composition for rectal delivery, 100 mg of a compound of Formula I-XV is mixed with 2.5 g of methylcellulose (1500 mPa), 100 mg of methylparapen, 5 g of glycerin and 100 mL of purified water. The resulting gel mixture is then incorporated into rectal delivery units, such as syringes, which are suitable for rectal administration.

Example 318 g Topical Gel Composition

To prepare a pharmaceutical topical gel composition, 100 mg of a compound of Formula I-XV is mixed with 1.75 g of hydroxypropyl cellulose, 10 mL of propylene glycol, 10 mL of isopropyl myristate and 100 mL of purified alcohol USP. The resulting gel mixture is then incorporated into containers, such as tubes, which are suitable for topical administration.

Example 318 h Ophthalmic Solution Composition

To prepare a pharmaceutical ophthalmic solution composition, 100 mg of a compound of Formula I-XV is mixed with 0.9 g of NaCl in 100 mL of purified water and filtered using a 0.2 micron filter. The resulting isotonic solution is then incorporated into ophthalmic delivery units, such as eye drop containers, which are suitable for ophthalmic administration.

Example 318i Nasal Spray Solution

To prepare a pharmaceutical nasal spray solution, 10 g of a compound of Formula I-XV is mixed with 30 mL of a 0.05M phosphate buffer solution (pH 4.4). The solution is placed in a nasal administrator designed to deliver 100 μl of spray for each application.

While some embodiments of the present disclosure have been shown and described herein, such embodiments are provided by way of example only. It is intended that the following claims define the scope of the present disclosure and that methods and structures within the scope of these claims and their equivalents be covered thereby. 

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and pharmaceutically acceptable salts thereof.
 130. The compound according to claim 129, selected from the group consisting of: Ex. Structure  40

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and pharmaceutically acceptable salts thereof.
 131. A pharmaceutical composition comprising a compound according to claim 129, or a pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable excipient, carrier, or binder.
 132. A method of treating a subject suffering from a CNS disorder or cancer, comprising administering to an individual in need of such treating a therapeutically effective amount of a compound of claim 129 or a pharmaceutically acceptable salt thereof.
 133. The method of claim 132, wherein the CNS disorder is schizophrenia, Alzheimer's disease, mild cognitive impairment, autism, an autism spectrum disorder, Fragile X syndrome, Retts syndrome, Asperger syndrome, Angelman syndrome, biopolar disorder, or depression.
 134. The method of claim 132, wherein cancer is selected from ovarian cancer, breast cancer, colorectal cancer, brain cancer, chronic myelogenous leukemia, renal cell carcinoma, gastric cancer, leukemia, lung cancer, melanoma, prostate cancer, T-cell lymphoma, heptocellular cancer, bladder cancer, kidney cancer, glioblastoma, mesothelioma, neuroma, meningioma, neuroblastoma, medulloblastoma, peripheral malignant nerve sheath tumor, ependymoma, chraniopharyngioma, astrocytoma, germinoma, glioma, mixed glioma, choroid plexus tumor, oligodendroglioma, peripheral neuroectodermal tumor, primitive neuroectodermal tumor (PNET), CNS lymphoma, pituitary adenoma, schwannoma, head and neck cancer, esophageal cancer, non-small cell lung cancer, small cell lung cancer, and mesothelioma.
 135. The method of claim 132, wherein cancer is a tumor associated with neurofibromatosis type 1 or type
 2. 136. The method of claim 135, wherein the tumor associated with neurofibromatosis type 1 or type 2 is a neurofibroma, optic glioma, malignant peripheral nerve sheath tumor, schwannoma, bilateral vestibular schwannoma, ependymoma, or meningioma. 